Secretion of recombinant proteins from E. coli – The Diggle Lab Sociomicrobiology

The microorganism Escherichia coli is usually used for recombinant protein manufacturing. Regardless of a number of advantageous traits like quick development and excessive protein yields, its incapability to simply secrete recombinant proteins into the extracellular medium stays a disadvantage for industrial manufacturing processes. To beat this limitation, a large number of approaches to reinforce the extracellular yield and the secretion effectivity of recombinant proteins have been developed in recent times.

Right here, a complete overview of secretion mechanisms for recombinant proteins from E. coli is given and divided into three fundamental sections. First, the construction of the E. coli cell envelope and the identified pure secretion programs are described. Second, the use and optimization of various one- or two-step secretion programs for recombinant protein manufacturing, in addition to additional permeabilization strategies are mentioned.

Lastly, the often-overlooked position of cell lysis in secretion research and its evaluation are addressed. Thus far, efficient approaches for rising the extracellular protein focus to greater than 10 g/L and virtually 100% secretion effectivity exist, nevertheless, the big vary of optimization strategies and their combos means that the potential for secretory protein manufacturing from E. coli has not but been totally realized.

Scalable Excessive-Efficiency Manufacturing of Recombinant Horseradish Peroxidase from E. coli Inclusion Our bodies

Horseradish peroxidase (HRP), an enzyme omnipresent in biotechnology, remains to be produced from furry root cultures, though this process is time-consuming and solely offers low yields. As well as, the plant-derived enzyme preparation consists of a variable combination of isoenzymes with excessive batch-to-batch variation stopping its use in therapeutic functions. On this research, we current a novel and scalable recombinant HRP manufacturing course of in Escherichia coli that yields a extremely pure, energetic and homogeneous single isoenzyme.

We efficiently developed a multi-step inclusion physique course of giving a last yield of 960 mg energetic HRP/L tradition medium with a purity of ≥99% decided by size-exclusion high-performance liquid chromatography (SEC-HPLC). The Reinheitszahl, in addition to the exercise with 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) and three,3′,5,5′-tetramethylbenzidine (TMB) as lowering substrates, are akin to commercially out there plant HRP. Thus, our preparation of recombinant, unglycosylated HRP from E. coli is a viable various to the enzyme from plant and extremely attention-grabbing for therapeutic functions.

Efficient Allitol Bioproduction from D-Fructose Catalyzed by Recombinant E. coli Complete Cells, and the Situation Optimization, Product Purification

Allitol is a sort of uncommon sugar alcohol with potential utility worth. An engineered pressure, which concurrently expressed D-psicose-3-epimerase (DPE), ribitol dehydrogenase (RDH), and formate dehydrogenase (FDH) three enzymes, was constructed by cloning above three genes into one plasmid and remodeled into the host E. coli pressure, and used because the whole-cell catalysts for biotransformation of allitol from the low-cost substrate of D-fructose. The entire cell allitol biotransformation circumstances have been optimized.

The medium, recombinant gene induction circumstances, and the substrate feeding fee for cultivation of the catalytic cells have been optimized. Then, the fed-batch tradition was made and scaled as much as 10 L fermentor. Lastly, 63.44 g/L allitol was obtained from 100 g/L D-fructose after Three h of biotransformation, and the allitol crystals of 99.9% purity have been obtained through the use of cooling recrystallization. The allitol manufacturing methodology developed on this analysis has excessive product purity, and is very environment friendly, simply scaled up, and appropriate for large-scale manufacturing of extremely purified allitol.

Scalable, two-stage, autoinduction of recombinant protein expression in E. coli using phosphate depletion

We report the scalable manufacturing of recombinant proteins in Escherichia coli, reliant on tightly managed autoinduction, triggered by phosphate depletion within the stationary section. The strategy, reliant on engineered strains and plasmids, allows improved protein expression throughout scales. Expression ranges utilizing this strategy have reached as excessive as 55% of the full mobile protein.

Secretion of recombinant proteins from E. coli

The preliminary use of the tactic in instrumented fed-batch fermentations allows cell densities of ∼30 gCDW/L and protein titers as much as 8.1 ± 0.7 g/L (∼270 mg/gCDW). The method has additionally been tailored to an optimized autoinduction media, enabling routine batch manufacturing at tradition volumes of 20 μl (384-well plates), 100 μl (96-well plates), 20 ml, and 100 ml. In batch cultures, cell densities routinely attain ∼5-7 gCDW/L, providing protein titers above 2 g/L. The methodology has been validated with a set of numerous heterologous proteins and is of basic use for the facile optimization of routine protein expression from excessive throughput screens to fed-batch fermentation.

A simplified methodology for producing laboratory grade recombinant TEV protease from E. coli.

The tobacco etch virus (TEV) protease has develop into a preferred selection for cleaving fusion proteins due to its excessive stringency in sequence recognition. Procedures for isolating recombinant protein from the cytoplasm of E. coli require rupturing of the cell wall by way of enzymatic therapy mixed with sonication or French press.

E. coli RuvC protein
01-011	Sceti	50 ug	EUR 574.8
Description: The E. coli RuvC protein is available in Europe and for worldwide shipping via Gentaur.

E. coli RuvC protein
01-012	Sceti	250 ug	EUR 1420.8
Description: The E. coli RuvC protein is available in Europe and for worldwide shipping via Gentaur.

RecA Protein E. coli
M1231-200	Biovision		EUR 242.4

Escherichia coli (E. coli) Antibody
abx415692-01mg	Abbexa	0.1 mg	EUR 760.8

Escherichia coli (E. coli) Antibody
abx415712-1ml	Abbexa	1 ml	EUR 727.2

Escherichia coli (E. coli) Antibody
abx411320-1ml	Abbexa	1 ml	EUR 610.8

E. coli antibody
10-E09C	Fitzgerald	100 ug	EUR 159.6
Description: Mouse monoclonal E. coli antibody

E. coli antibody
10-E10A	Fitzgerald	500 ug	EUR 591.6
Description: Mouse monoclonal E. coli antibody

E. coli antibody
10-E10B	Fitzgerald	500 ug	EUR 591.6
Description: Mouse monoclonal E. coli antibody

E. coli antibody
20C-CR1244GP	Fitzgerald	1 ml	EUR 561.6
Description: Goat polyclonal E. coli antibody

E. coli antibody
20-ER13	Fitzgerald	1 ml	EUR 426
Description: Rabbit polyclonal E. coli antibody

E. coli antibody
10C-CR1244M1	Fitzgerald	100 ug	EUR 489.6
Description: Mouse monoclonal E. coli antibody

E. coli Antibody
abx021669-1mg	Abbexa	1 mg	EUR 1554

E. coli Antibody
abx021670-1mg	Abbexa	1 mg	EUR 1045.2

E. coli Antibody
abx021671-02ml	Abbexa	0.2 ml	EUR 594

E. coli Antibody
abx021672-1mg	Abbexa	1 mg	EUR 1045.2

E. coli Antibody
abx021673-1mg	Abbexa	1 mg	EUR 1045.2

E. coli Antibody
abx022970-1ml	Abbexa	1 ml	EUR 878.4

E. coli Antibody
abx022971-1ml	Abbexa	1 ml	EUR 1161.6

E. coli Antibody
abx022975-1ml	Abbexa	1 ml	EUR 718.8

E. coli Antibody
abx022977-1ml	Abbexa	1 ml	EUR 811.2

E. coli GroL
DAG1704	Creative Diagnostics	100 ug	EUR 1098

E. coli GroS
DAG1705	Creative Diagnostics	100 ug	EUR 1098

E. coli DsbA
DAG1712	Creative Diagnostics	100 ug	EUR 1098

E. coli GrpE
DAG1714	Creative Diagnostics	100 ug	EUR 1232.4

Recombinant Trypanosoma Cruzi Shed-acute-phase-antigen Protein (aa 61-460) (E. coli) [His]
VAng-Wyb8480-100g	Creative Biolabs	100 µg	EUR 4002
Description: Trypanosoma Cruzi Shed-acute-phase-antigen protein, recombinant protein.

Recombinant Trypanosoma Cruzi Shed-acute-phase-antigen Protein (aa 61-460) (E. coli) [His]
VAng-Wyb8480-500g	Creative Biolabs	500 µg	EUR 9794.4
Description: Trypanosoma Cruzi Shed-acute-phase-antigen protein, recombinant protein.

Escherichia coli (E. coli) Antibody (Biotin)
abx415713-1ml	Abbexa	1 ml	EUR 861.6

Escherichia coli (E. coli) Antibody (Biotin)
abx411321-1ml	Abbexa	1 ml	EUR 710.4

Escherichia coli (E. coli) Antibody (FITC)
abx411322-1ml	Abbexa	1 ml	EUR 610.8

E. Coli O 157 Protein
abx061475-01ml	Abbexa	0.1 ml	EUR 661.2

E. Coli Recombinant TRXB
6331-100	Biovision		EUR 510

E. Coli Recombinant SlyD
6366-100	Biovision		EUR 548.4

E. coli antibody (FITC)
60-E13	Fitzgerald	1 ml	EUR 453.6
Description: Rabbit polyclonal E. coli antibody (FITC) conjugated

E. coli antibody (biotin)
60-E13B	Fitzgerald	1 ml	EUR 561.6
Description: Rabbit polyclonal E. coli antibody (biotin) conjugated

E. coli antibody (HRP)
60-E14	Fitzgerald	1 ml	EUR 489.6
Description: Rabbit polyclonal E. coli antibody (HRP) conjugated

E. coli O157 antibody
10-1490	Fitzgerald	100 ug	EUR 163.2
Description: Mouse monoclonal E. coli O157 antibody

E. coli O157 antibody
10-1521	Fitzgerald	100 ug	EUR 170.4
Description: Mouse monoclonal E. coli O157 antibody

E. coli O157 antibody
10-E12A	Fitzgerald	100 ug	EUR 462
Description: Mouse monoclonal E. coli O157 antibody

E. coli DnaJ Antibody
32022-05111	AssayPro	150 ug	EUR 313.2

E. coli pykF Antibody
32163-05111	AssayPro	150 ug	EUR 313.2

E. coli GOR Antibody
32540-05111	AssayPro	150 ug	EUR 313.2

E. coli CysH Antibody
30281-05111	AssayPro	150 ug	EUR 313.2

E. Coli HCP antibody
20R-EG001	Fitzgerald	1 ml	EUR 1004.4
Description: Goat polyclonal E. Coli HCP antibody

E. coli NDK Antibody
32662-05111	AssayPro	150 ug	EUR 313.2

E. coli PPA Antibody
32678-05111	AssayPro	150 ug	EUR 313.2

E. coli glk Antibody
32864-05111	AssayPro	150 ug	EUR 313.2

E. Coli ldhA Antibody
32898-05111	AssayPro	150 ug	EUR 313.2

E. coli deoC Antibody
32936-05111	AssayPro	150 ug	EUR 313.2

E. coli Skp Antibody
32971-05111	AssayPro	150 ug	EUR 313.2

E. coli nanA Antibody
33092-05111	AssayPro	150 ug	EUR 313.2

E. coli O157 antibody
10C-CR1295M3	Fitzgerald	100 ug	EUR 470.4
Description: Mouse monoclonal E. coli O157 antibody

E. coli LexA repressor
01-005	Sceti	50 ug	EUR 574.8
Description: The E. coli LexA repressor is available in Europe and for worldwide shipping via Gentaur.

E. coli LexA repressor
01-006	Sceti	250 ug	EUR 1420.8
Description: The E. coli LexA repressor is available in Europe and for worldwide shipping via Gentaur.

TesA, E. coli esterase
0306-1	AthenaES	100 Units	EUR 505.2

TesA, E. coli esterase
0306-5	AthenaES	500 Units	EUR 2046

E. coli GroEL Antibody
20012-05011	AssayPro	150 ug	EUR 260.4

E. coli O157 Antibody
abx021684-1mg	Abbexa	1 mg	EUR 1220.4

E. coli O157 Antibody
abx021685-1mg	Abbexa	1 mg	EUR 1195.2

E. coli O157 Antibody
abx021686-1mg	Abbexa	1 mg	EUR 1195.2

E. coli O157 Antibody
abx021687-1mg	Abbexa	1 mg	EUR 1195.2

E. coli O157 Antibody
abx021688-1mg	Abbexa	1 mg	EUR 1128

E. coli O157 Antibody
abx021689-1mg	Abbexa	1 mg	EUR 1195.2

E. coli Antibody (HRP)
abx022972-1ml	Abbexa	1 ml	EUR 718.8

E. coli Antibody (FITC)
abx022974-1ml	Abbexa	1 ml	EUR 777.6

E. coli Antibody (Biotin)
abx022976-1ml	Abbexa	1 ml	EUR 718.8

E. coli Antibody (HRP)
abx022978-1ml	Abbexa	1 ml	EUR 1053.6

E. coli Antibody (Biotin)
abx022979-1ml	Abbexa	1 ml	EUR 886.8

E. coli O157 Antibody
abx024017-1mg	Abbexa	1 mg	EUR 1212

E. coli O 157
abx291002-100mg	Abbexa	100 mg	EUR 744

Benzonase, E. Coli Recombinant
7680-25	Biovision		EUR 398.4

Benzonase, E. Coli Recombinant
7680-5	Biovision		EUR 164.4

E. coli FimC [His]
DAG1666	Creative Diagnostics	100 ug	EUR 1232.4

E. coli Eco [His]
DAG1667	Creative Diagnostics	50 ug	EUR 1232.4

E. coli DNA Ligase
M1217-100	Biovision		EUR 385.2

E. coli Exonuclease I
M1222-100	Biovision		EUR 301.2

E. coli Exonuclease III
M1223-100	Biovision		EUR 320.4

E. coli Recombinant IDNK
P1050-10	Biovision		EUR 196.8

E. coli Recombinant IDNK
P1050-50	Biovision		EUR 457.2

Recombinant E. coli, Glucokinase
P1054-10	Biovision		EUR 196.8

Recombinant E. coli, Glucokinase
P1054-50	Biovision		EUR 457.2

Enterotoxin STp (E. coli)
H-6248.0500	Bachem	0.5mg	EUR 835.2
Description: Sum Formula: C81H110N20O26S6; CAS# [115474-04-9] net

Enterotoxin STp (E. coli)
H-6248.1000	Bachem	1.0mg	EUR 1416
Description: Sum Formula: C81H110N20O26S6; CAS# [115474-04-9] net

E. coli Topoisomerase IV
TG2000EC-1	TopoGen	250 units	EUR 537.6

E. coli Topoisomerase IV
TG2000EC-3	TopoGen	500 units	EUR 819.6

E. coli Topoisomerase IV
TG2000EC-5	TopoGen	1000 units	EUR 1262.4

E. coli DNA Gyrase
TG2000G-1	TopoGen	100 units	EUR 388.8

E. coli DNA Gyrase
TG2000G-3	TopoGen	500 units	EUR 846

E. coli DNA Gyrase
TG2000G-5	TopoGen	1000 units	EUR 1396.8

E. coli DNA Gyrase
TG2000G-7	TopoGen	2000 units	EUR 2203.2

E. coli Protein GrpE (GrpE) Protein
20-abx261718	Abbexa	EUR 4236.00 EUR 393.60 EUR 276.00	1 mg 25 ug 5 ug

Human Escherichia coli (E. coli) ELISA Kit
abx052493-96tests	Abbexa	96 tests	EUR 801.6

Cow Escherichia coli (E. coli) ELISA Kit
abx055785-96tests	Abbexa	96 tests	EUR 801.6

CLIA kit for Human TPS (Tissue Polypeptide Specific Antigen)
E-CL-H1374	Elabscience Biotech	1 plate of 96 wells	EUR 700.8

Description: A sandwich CLIA kit for quantitative measurement of Human TPS (Tissue Polypeptide Specific Antigen) in samples from Serum, Plasma, Cell supernatant

ELISA kit for Human TPS (Tissue Polypeptide Specific Antigen)
E-EL-H2362	Elabscience Biotech	1 plate of 96 wells	EUR 640.8

Description: A sandwich ELISA kit for quantitative measurement of Human TPS (Tissue Polypeptide Specific Antigen) in samples from Serum, Plasma, Cell supernatant

E. Coli Proteins-Agarose affinity gel for removing E. coli antibodies
EC11-G	Alpha Diagnostics	1 ml	EUR 343.2

E. coli 0157:H7 cell protein
30-AE13	Fitzgerald	1 ml	EUR 340.8
Description: Heat inactivated E. coli O157:H7 cells

E. coli Syndecan 1 (SDC1) Protein
20-abx263542	Abbexa	EUR 994.80 EUR 493.20 EUR 644.40	10 ug 2 µg 5 ug

E. coli Progesterone Receptor (PGR) Protein
20-abx263552	Abbexa	EUR 994.80 EUR 493.20 EUR 644.40	10 ug 2 µg 5 ug

Recombinant Human RAGE Protein (E. coli)
VAng-2906Lsx-1mg	Creative Biolabs	1 mg	EUR 4316.4
Description: Recombinant Human RAGE (AGER) protein is expressed in E. coli. (Uniprot ID: Q15109)

Recombinant Human RAGE Protein (E. coli)
VAng-2906Lsx-200g	Creative Biolabs	200 µg	EUR 1594.8
Description: Recombinant Human RAGE (AGER) protein is expressed in E. coli. (Uniprot ID: Q15109)

Recombinant Human RAGE Protein (E. coli)
VAng-2906Lsx-500g	Creative Biolabs	500 µg	EUR 2749.2
Description: Recombinant Human RAGE (AGER) protein is expressed in E. coli. (Uniprot ID: Q15109)

Recombinant CMV Protein (E. coli) [GST]
VAng-Lsx0035-inquire	Creative Biolabs	inquire	Ask for price
Description: CMV [GST], recombinant protein from E. coli.

Recombinant Trypanosoma Cruiz Protein (E. coli)
VAng-Wyb8373-1mg	Creative Biolabs	1 mg	EUR 4431.6
Description: Trypanosoma Cruiz protein, recombinant protein.

Recombinant HIV-2 E gp 36 Protein (E. coli)
VAng-0534Lsx-inquire	Creative Biolabs	inquire	Ask for price
Description: HIV type 2 Envelope glycoprotein 36, recombinant protein from E. coli. MW 16 kDa, 1 mg/mL.

Deoxybrevianamide E
D083-1MG	TOKU-E	1 mg	EUR 333.6

Deoxybrevianamide E
D083-5MG	TOKU-E	5 mg	EUR 1076.4

Cytochalasin E
C197-1MG	TOKU-E	1 mg	EUR 157.2

Cytochalasin E
C197-5MG	TOKU-E	5 mg	EUR 462

Paraherquamide E
P070-0.5MG	TOKU-E	0.5 mg	EUR 480

Paraherquamide E
P070-2.5MG	TOKU-E	2.5 mg	EUR 1590

Phenelfamycin E
P078-1MG	TOKU-E	1 mg	EUR 381.6

Phenelfamycin E
P078-5MG	TOKU-E	5 mg	EUR 1248

Oligomycin E
O021-1MG	TOKU-E	1 mg	EUR 381.6

Oligomycin E
O021-5MG	TOKU-E	5 mg	EUR 1248

Roquefortine E
R035-1MG	TOKU-E	1 mg	EUR 268.8

Roquefortine E
R035-5MG	TOKU-E	5 mg	EUR 849.6

Recombinant Trypanosoma Brucei Variant-specific antigen Protein (aa 23-367) (E. coli) [His]
VAng-Wyb8311-100g	Creative Biolabs	100 µg	EUR 4002
Description: Trypanosoma Brucei brucei Variant-specific antigen protein, recombinant protein.

Recombinant Trypanosoma Brucei Variant-specific antigen Protein (aa 23-367) (E. coli) [His]
VAng-Wyb8311-500g	Creative Biolabs	500 µg	EUR 9794.4
Description: Trypanosoma Brucei brucei Variant-specific antigen protein, recombinant protein.

Recombinant Plasmodium Falciparum GTP-binding nuclear protein Ran Protein (aa 1-214) (E. coli)
VAng-Wyb6469-inquire	Creative Biolabs	inquire	Ask for price
Description: Plasmodium Falciparum GTP-binding nuclear protein Ran, recombinant protein.

E. coli Chaperone Protein Dnaj (DNAJ) Protein
20-abx260479	Abbexa	EUR 276.00 EUR 2798.40 EUR 393.60	10 ug 1 mg 50 ug

E. coli Chaperone Protein SKP (SKP) Protein
20-abx260554	Abbexa	EUR 3433.20 EUR 393.60 EUR 276.00	1 mg 25 ug 5 ug

E. coli Green Fluorescent Protein (GFP) Protein
20-abx260632	Abbexa	EUR 276.00 EUR 3650.40 EUR 393.60	10 ug 1 mg 50 ug

Purified Rec. E. Coli Thioredoxin (TrxA, E. coli) (fusion protein) for WB +ve control
THRX11-C	Alpha Diagnostics	100 ul	EUR 343.2

E. coli DNA Primase Assay Kit Plus (E. coli primase-helicase included)
EGA100KE	ProFoldin	100 assays	EUR 444.91
Description: This product includes all the kit components in E. coli DNA Primase Assay Kit (Catalog No. EGA100K ) plus 45 ul of 100 x E. coli primase-helicase complex. It is for 100 assays of E.coli DNA primase reactions in a 96-well plate format or 200 assays in 384-well assay format.

E. coli RNA polymerase Assay Kit Plus (E. coli RNA polymerase included)
RPA100KE	ProFoldin	100 assays	EUR 467.15
Description: This product includes all the reagents in E. coli RNA Polymerase Assay Kit (Catalog number RPA100K) plus the enzyme, 33 ul of 100 x E. coli RNAP. It is for 100 assays of E. coli RNA polymerase reactions in a 384-well assay format.

Escherichia coli Serotype O2 (E. coli O2) Antibody
abx411330-02mg	Abbexa	0.2 mg	EUR 678

Escherichia coli Serotype O15 (E. coli O15) Antibody
abx411331-02mg	Abbexa	0.2 mg	EUR 678

Escherichia coli Serotype O20 (E. coli O20) Antibody
abx411332-02mg	Abbexa	0.2 mg	EUR 678

E. coli O157 antibody (FITC)
61C-CR1295M1F	Fitzgerald	100 ug	EUR 489.6
Description: Mouse monoclonal E. coli O157 antibody (FITC)

E. coli O157 antibody (FITC)
61C-CR1295M4F	Fitzgerald	100 ug	EUR 561.6
Description: Mouse monoclonal E. coli O157 antibody (FITC)

E. Coli Recombinant Thioredoxin 1
6329-50	Biovision		EUR 483.6

E. coli O157:H7 antibody
70-XG13	Fitzgerald	1 mg	EUR 734.4
Description: Affinity purified Goat polyclonal E. coli O157:H7 antibody.

E. coli antibody (K99 Pilus)
10-E20A	Fitzgerald	500 ug	EUR 591.6
Description: Mouse monoclonal E. coli antibody (K99 Pilus)

Insulin, human recombinant (E. coli)
4772-25	Biovision		EUR 542.4

Insulin, human recombinant (E. coli)
4772-5	Biovision		EUR 248.4

E. coli Tryptophanase (Tnase) Antibody
33517-05111	AssayPro	150 ug	EUR 313.2

E. coli Glycerol Kinase Antibody
32699-05111	AssayPro	150 ug	EUR 313.2

Mouse antibody for E. coli
1063	Virostat	100 ug	EUR 438.46
Description: This is Mouse monoclonal FITC conjugated antibody against E coli O157 for WB, ELISA.

E. coli RecQ DNA helicase
01-003	Sceti	50 ug	EUR 574.8
Description: The E. coli RecQ DNA helicase is available in Europe and for worldwide shipping via Gentaur.

E. coli RecQ DNA helicase
01-004	Sceti	250? g	EUR 1858.8
Description: The E. coli RecQ DNA helicase is available in Europe and for worldwide shipping via Gentaur.

E. coli Enterotoxin B Antibody
abx021678-1mg	Abbexa	1 mg	EUR 718.8

E. coli Enterotoxin B Antibody
abx021679-1mg	Abbexa	1 mg	EUR 727.2

E. coli Enterotoxin B Antibody
abx021680-1mg	Abbexa	1 mg	EUR 718.8

E. coli O157 Antibody (FITC)
abx021683-1mg	Abbexa	1 mg	EUR 2798.4

E. coli O157:H7 Antibody
abx022980-1mg	Abbexa	1 mg	EUR 718.8

E. coli Enterotoxin LT Antibody
abx023834-1mg	Abbexa	1 mg	EUR 878.4

E. coli Enterotoxin LT Antibody
abx023835-1mg	Abbexa	1 mg	EUR 878.4

E. coli Enterotoxin LT Antibody
abx023836-1mg	Abbexa	1 mg	EUR 878.4

E. coli Enterotoxin LT Antibody
abx023837-1mg	Abbexa	1 mg	EUR 878.4

E. coli Enterotoxin STp Antibody
abx023838-1mg	Abbexa	1 mg	EUR 1053.6

E. coli Enterotoxin STp Antibody
abx023839-1mg	Abbexa	1 mg	EUR 1053.6

E. coli Enterotoxin STp Antibody
abx023840-1mg	Abbexa	1 mg	EUR 1053.6

rHu BMP-2 (E. coli)
AK8356-0002	Akron Biotech	2µg	Ask for price

rHu BMP-2 (E. coli)
AK8356-0010	Akron Biotech	10µg	Ask for price

rHu BMP-2 (E. coli)
AK8356-0100	Akron Biotech	100µg	Ask for price

rHu BMP-2 (E. coli)
AK8356-1000	Akron Biotech	1mg	Ask for price

E. coli O157 (9.88) Antibody
BNC040207-100	Biotium	100uL	EUR 238.8
Description: Primary antibody against E. coli O157 (9.88),CF405S conjugate, Concentration: 0.1mg/mL

E. coli O157 (9.88) Antibody
BNC040207-500	Biotium	500uL	EUR 652.8
Description: Primary antibody against E. coli O157 (9.88),CF405S conjugate, Concentration: 0.1mg/mL

E. coli O157 (9.88) Antibody
BNC050207-100	Biotium	100uL	EUR 238.8
Description: Primary antibody against E. coli O157 (9.88),CF405M conjugate, Concentration: 0.1mg/mL

E. coli O157 (9.88) Antibody
BNC050207-500	Biotium	500uL	EUR 652.8
Description: Primary antibody against E. coli O157 (9.88),CF405M conjugate, Concentration: 0.1mg/mL

E. coli β-gal peptide
DAG2441	Creative Diagnostics	0.05 mg	EUR 582

Native E. Coli O 158
DAGA-3025	Creative Diagnostics	0.1 ml	EUR 858

E. coli O157 (9.88) Antibody
BNCAP0207-100	Biotium	100uL	EUR 238.8
Description: Primary antibody against E. coli O157 (9.88),Alkaline Phosphatase conjugate, Concentration: 0.1mg/mL

E. coli O157 (9.88) Antibody
BNCAP0207-500	Biotium	500uL	EUR 652.8
Description: Primary antibody against E. coli O157 (9.88),Alkaline Phosphatase conjugate, Concentration: 0.1mg/mL

E. coli O157 (9.88) Antibody
BNCB0207-100	Biotium	100uL	EUR 238.8
Description: Primary antibody against E. coli O157 (9.88),Biotin conjugate, Concentration: 0.1mg/mL

E. coli O157 (9.88) Antibody
BNCB0207-500	Biotium	500uL	EUR 652.8
Description: Primary antibody against E. coli O157 (9.88),Biotin conjugate, Concentration: 0.1mg/mL

E. coli O157 (9.88) Antibody
BNCP0207-250	Biotium	250uL	EUR 459.6
Description: Primary antibody against E. coli O157 (9.88),PerCP conjugate, Concentration: 0.1mg/mL

E. coli O157 (9.88) Antibody
BNCR0207-250	Biotium	250uL	EUR 459.6
Description: Primary antibody against E. coli O157 (9.88),RPE conjugate, Concentration: 0.1mg/mL

E. coli O157 (9.88) Antibody
BNC940207-100	Biotium	100uL	EUR 238.8
Description: Primary antibody against E. coli O157 (9.88),CF594 conjugate, Concentration: 0.1mg/mL

E. coli O157 (9.88) Antibody
BNC940207-500	Biotium	500uL	EUR 652.8
Description: Primary antibody against E. coli O157 (9.88),CF594 conjugate, Concentration: 0.1mg/mL

E. coli O157 (9.88) Antibody
BNCA0207-250	Biotium	250uL	EUR 459.6
Description: Primary antibody against E. coli O157 (9.88),APC conjugate, Concentration: 0.1mg/mL

E. coli O157 (9.88) Antibody
BNC810207-100	Biotium	100uL	EUR 238.8
Description: Primary antibody against E. coli O157 (9.88),CF680R conjugate, Concentration: 0.1mg/mL

E. coli O157 (9.88) Antibody
BNC810207-500	Biotium	500uL	EUR 652.8
Description: Primary antibody against E. coli O157 (9.88),CF680R conjugate, Concentration: 0.1mg/mL

E. coli O157 (9.88) Antibody
BNUB0207-100	Biotium	100uL	EUR 250.8
Description: Primary antibody against E. coli O157 (9.88), Concentration: 0.2mg/mL

E. coli O157 (9.88) Antibody
BNUB0207-500	Biotium	500uL	EUR 549.6
Description: Primary antibody against E. coli O157 (9.88), Concentration: 0.2mg/mL

E. coli O157 (9.88) Antibody
BNCH0207-100	Biotium	100uL	EUR 238.8
Description: Primary antibody against E. coli O157 (9.88),Horseradish Peroxidase conjugate, Concentration: 0.1mg/mL

E. coli O157 (9.88) Antibody
BNCH0207-500	Biotium	500uL	EUR 652.8
Description: Primary antibody against E. coli O157 (9.88),Horseradish Peroxidase conjugate, Concentration: 0.1mg/mL

E. coli O157 (9.88) Antibody
BNC880207-100	Biotium	100uL	EUR 238.8
Description: Primary antibody against E. coli O157 (9.88),CF488A conjugate, Concentration: 0.1mg/mL

E. coli O157 (9.88) Antibody
BNC880207-500	Biotium	500uL	EUR 652.8
Description: Primary antibody against E. coli O157 (9.88),CF488A conjugate, Concentration: 0.1mg/mL

E. coli O157 (9.88) Antibody
BNC680207-100	Biotium	100uL	EUR 238.8
Description: Primary antibody against E. coli O157 (9.88),CF568 conjugate, Concentration: 0.1mg/mL

E. coli O157 (9.88) Antibody
BNC680207-500	Biotium	500uL	EUR 652.8
Description: Primary antibody against E. coli O157 (9.88),CF568 conjugate, Concentration: 0.1mg/mL

E. coli O157 (9.88) Antibody
BNC470207-100	Biotium	100uL	EUR 238.8
Description: Primary antibody against E. coli O157 (9.88),CF647 conjugate, Concentration: 0.1mg/mL

E. coli O157 (9.88) Antibody
BNC470207-500	Biotium	500uL	EUR 652.8
Description: Primary antibody against E. coli O157 (9.88),CF647 conjugate, Concentration: 0.1mg/mL

E. coli O157 (9.88) Antibody
BNC430207-100	Biotium	100uL	EUR 238.8
Description: Primary antibody against E. coli O157 (9.88),CF543 conjugate, Concentration: 0.1mg/mL

E. coli O157 (9.88) Antibody
BNC430207-500	Biotium	500uL	EUR 652.8
Description: Primary antibody against E. coli O157 (9.88),CF543 conjugate, Concentration: 0.1mg/mL

E. coli O157 (9.88) Antibody
BNC610207-100	Biotium	100uL	EUR 238.8
Description: Primary antibody against E. coli O157 (9.88),CF660R conjugate, Concentration: 0.1mg/mL

E. coli O157 (9.88) Antibody
BNC610207-500	Biotium	500uL	EUR 652.8
Description: Primary antibody against E. coli O157 (9.88),CF660R conjugate, Concentration: 0.1mg/mL

E. coli O157 (9.88) Antibody
BNC800207-100	Biotium	100uL	EUR 238.8
Description: Primary antibody against E. coli O157 (9.88),CF680 conjugate, Concentration: 0.1mg/mL

E. coli O157 (9.88) Antibody
BNC800207-500	Biotium	500uL	EUR 652.8
Description: Primary antibody against E. coli O157 (9.88),CF680 conjugate, Concentration: 0.1mg/mL

E. coli O157 (9.88) Antibody
BNC400207-100	Biotium	100uL	EUR 238.8
Description: Primary antibody against E. coli O157 (9.88),CF640R conjugate, Concentration: 0.1mg/mL

E. coli O157 (9.88) Antibody
BNC400207-500	Biotium	500uL	EUR 652.8
Description: Primary antibody against E. coli O157 (9.88),CF640R conjugate, Concentration: 0.1mg/mL

E. coli O157 (9.88) Antibody
BNC550207-100	Biotium	100uL	EUR 238.8
Description: Primary antibody against E. coli O157 (9.88),CF555 conjugate, Concentration: 0.1mg/mL

E. coli O157 (9.88) Antibody
BNC550207-500	Biotium	500uL	EUR 652.8
Description: Primary antibody against E. coli O157 (9.88),CF555 conjugate, Concentration: 0.1mg/mL

E. coli O157 (9.88) Antibody
BNC700207-100	Biotium	100uL	EUR 238.8
Description: Primary antibody against E. coli O157 (9.88),CF770 conjugate, Concentration: 0.1mg/mL

E. coli O157 (9.88) Antibody
BNC700207-500	Biotium	500uL	EUR 652.8
Description: Primary antibody against E. coli O157 (9.88),CF770 conjugate, Concentration: 0.1mg/mL

E. coli O157 (9.88) Antibody
BNUM0207-50	Biotium	50uL	EUR 474
Description: Primary antibody against E. coli O157 (9.88), 1mg/mL

Uricase from E. coli, Recombinant
DIA-415	Creative Enzymes	5KU	EUR 453.6

E. coli DNA Polymerase I
M1210-100	Biovision		EUR 307.2

E. coli Recombinant Carbonic anhydrase
P1049-10	Biovision		EUR 196.8

E. coli Recombinant Carbonic anhydrase
P1049-50	Biovision		EUR 457.2

Recombinant Diacylglycerol Kinase (E. coli)
MET-5035	Cell Biolabs	100 mg	EUR 331.2
Description: Purified recombinant prokaryotic DAG kinase expressed in E. coli

Recombinant Diacylglycerol Kinase (E. coli)
MET-5035-5	Cell Biolabs	5 x 100 mg	EUR 1104
Description: Purified recombinant prokaryotic DAG kinase expressed in E. coli

E. coli/Coliform Count Plate
FSA1010	Scientific Laboratory Supplies	PK500	EUR 670.32

E. coli/Coliform Count Plate
FSA1012	Scientific Laboratory Supplies	PK50	EUR 80.94

Small Nuclear Ribonucleoprotein Polypeptide E Protein
20-abx263278	Abbexa	EUR 276.00 EUR 1930.80 EUR 393.60	1 µg 50 ug 5 ug

Mouse Anti-E. coli and LPS IgG1 Monoclonal Antibody, Clone 1C6
7098	Chondrex	1 mg/ml x 0.1 ml	EUR 578.52
Description: Mouse Anti-E. coli and LPS IgG1 Monoclonal Antibody

Mouse Anti-E. coli and LPS IgG2a Monoclonal Antibody, Clone 1E1
7099	Chondrex	1 mg/ml x 0.1 ml	EUR 578.52
Description: Mouse Anti-E. coli and LPS IgG2a Monoclonal Antibody

Mouse Anti-E. coli and LPS IgG2b Monoclonal Antibody, Clone 2H10
7100	Chondrex	1 mg/ml x 0.1 ml	EUR 578.52
Description: Mouse Anti-E. coli and LPS IgG2b Monoclonal Antibody

Recombinant Trypanosoma Brucei Proliferating cell nuclear antigen Protein (aa 21-292) (E. coli) [His]
VAng-Wyb8157-100g	Creative Biolabs	100 µg	EUR 4002
Description: Trypanosoma Brucei gambiense Proliferating cell nuclear antigen protein, recombinant protein.

Recombinant Trypanosoma Brucei Proliferating cell nuclear antigen Protein (aa 21-292) (E. coli) [His]
VAng-Wyb8157-500g	Creative Biolabs	500 µg	EUR 9794.4
Description: Trypanosoma Brucei gambiense Proliferating cell nuclear antigen protein, recombinant protein.

Recombinant Trypanosoma Cruzi Proliferating cell nuclear antigen Protein (aa 24-289) (E. coli) [His]
VAng-Wyb8466-100g	Creative Biolabs	100 µg	EUR 4002
Description: Trypanosoma Cruzi Proliferating cell nuclear antigen protein, recombinant protein.

Recombinant Trypanosoma Cruzi Proliferating cell nuclear antigen Protein (aa 24-289) (E. coli) [His]
VAng-Wyb8466-500g	Creative Biolabs	500 µg	EUR 9794.4
Description: Trypanosoma Cruzi Proliferating cell nuclear antigen protein, recombinant protein.

Recombinant (E. coli) Human neuron-specific enolase (NSE/gamma enolase) protein control for western
NSE11-C	Alpha Diagnostics	100 ul	EUR 343.2

Recombinant (E. coli) Human neuron-specific enolase (NSE/gamma enolase) protein control for western
NSE16-R-10	Alpha Diagnostics	10 ug	EUR 416.4

Recombinant Trypanosoma Cruzi Insect stage-specific antigen Protein (aa 61-460) (E. coli) [His]
VAng-Wyb8440-100g	Creative Biolabs	100 µg	EUR 4002
Description: Trypanosoma Cruzi Insect stage-specific antigen protein, recombinant protein.

Recombinant Trypanosoma Cruzi Insect stage-specific antigen Protein (aa 61-460) (E. coli) [His]
VAng-Wyb8440-500g	Creative Biolabs	500 µg	EUR 9794.4
Description: Trypanosoma Cruzi Insect stage-specific antigen protein, recombinant protein.

E. coli DNA Topoisomerase IV Assay Kit Plus (E. coli Topo IV included)
DDC020KE	ProFoldin	20 assays	EUR 221.78
Description: This product includes all the components in DNA Topoisomerase IV Assay Kit (Catalog No. DDC020K)plus 12ul of 100 x E.coli topoisomerase IV (500 nM) for 20 E. coli topoisomerase IV DNA decatenation assays.

E. coli DNA Topoisomerase I Assay Kit Plus (E. coli Topo I included)
DRA020KE	ProFoldin	20 assays	EUR 221.78
Description: This product includes 160 ul of 10 x Buffer T1, 84 ul of 10 x supercoiled plasmid DNA, 10 ul of 100 x E. coli topoisomerase I, 8 ul of 1500 x Dye H19 and 500 ul of 10 x H19 dilution buffer. It is for 20 DNA relaxation assays in a 96-well plate format or 40 assays in a 384-well assay format.

E. coli DNA Topoisomerase II (Gyrase) Assay Kit Plus (E. coli gyrase included)
DSA020KE	ProFoldin	20 assays	EUR 221.78
Description: This product includes160 ul of 10 x Buffer T2, 84 ul of 10 x relaxed DNA, 6 ul of 1500 x H19 dye, 90 ul of 10 x ATP and 550 ul of 10 x H19 dilution buffer. It is for 20 assays in 96-well plate format. E. coli gyrase is not included.

E. coli NAD+-dependent DNA ligase assay kit Plus (E. coli ligase included)
NLA100KE	ProFoldin	100 assays	EUR 444.91
Description: This product includes 800 ul of 10 x buffer, 44 ul of 100x DNA substrate, 44 ul of 100 x NAD+, 21 ml of Reagent T, 10 ul of 500 x E. coli ligase and 220 ul of 10 x fluorescence dye for 100 assays.

E. coli Maltose Binding Protein (MBP) Antibody
35738-05111	AssayPro	150 ug	EUR 313.2

Right here we current an expedited methodology for producing laboratory-grade TEV protease in E. coli utilizing a freeze-thaw methodology, adopted by purification with immobilized metallic affinity chromatography. Protease is obtained by expression from the pDZ2087 plasmid in BL21 (DE3) cells. Proteolysis ensuing from this product, cleaves a maltose-binding protein fusion to completion at a fusion-to-protease molar ratio of 50:1.

Scalable Excessive-Efficiency Manufacturing of Recombinant Horseradish Peroxidase from E. coli Inclusion Our bodies

Efficient Allitol Bioproduction from D-Fructose Catalyzed by Recombinant E. coli Complete Cells, and the Situation Optimization, Product Purification

Scalable, two-stage, autoinduction of recombinant protein expression in E. coli using phosphate depletion

A simplified methodology for producing laboratory grade recombinant TEV protease from E. coli.

E. coli RuvC protein

E. coli RuvC protein

RecA Protein E. coli

Escherichia coli (E. coli) Antibody

Escherichia coli (E. coli) Antibody

Escherichia coli (E. coli) Antibody

E. coli antibody

E. coli antibody

E. coli antibody

E. coli antibody

E. coli antibody

E. coli antibody

E. coli Antibody

E. coli Antibody

E. coli Antibody

E. coli Antibody

E. coli Antibody

E. coli Antibody

E. coli Antibody

E. coli Antibody

E. coli Antibody

E. coli GroL

E. coli GroS

E. coli DsbA

E. coli GrpE

Recombinant Trypanosoma Cruzi Shed-acute-phase-antigen Protein (aa 61-460) (E. coli) [His]

Recombinant Trypanosoma Cruzi Shed-acute-phase-antigen Protein (aa 61-460) (E. coli) [His]

Escherichia coli (E. coli) Antibody (Biotin)

Escherichia coli (E. coli) Antibody (Biotin)

Escherichia coli (E. coli) Antibody (FITC)

E. Coli O 157 Protein

E. Coli Recombinant TRXB

E. Coli Recombinant SlyD

E. coli antibody (FITC)

E. coli antibody (biotin)

E. coli antibody (HRP)

E. coli O157 antibody

E. coli O157 antibody

E. coli O157 antibody

E. coli DnaJ Antibody

E. coli pykF Antibody

E. coli GOR Antibody

E. coli CysH Antibody

E. Coli HCP antibody

E. coli NDK Antibody

E. coli PPA Antibody

E. coli glk Antibody

E. Coli ldhA Antibody

E. coli deoC Antibody

E. coli Skp Antibody

E. coli nanA Antibody

E. coli O157 antibody

E. coli LexA repressor

E. coli LexA repressor

TesA, E. coli esterase

TesA, E. coli esterase

E. coli GroEL Antibody

E. coli O157 Antibody

E. coli O157 Antibody

E. coli O157 Antibody

E. coli O157 Antibody

E. coli O157 Antibody

E. coli O157 Antibody

E. coli Antibody (HRP)

E. coli Antibody (FITC)

E. coli Antibody (Biotin)

E. coli Antibody (HRP)

E. coli Antibody (Biotin)

E. coli O157 Antibody

E. coli O 157

Benzonase, E. Coli Recombinant

Benzonase, E. Coli Recombinant

E. coli FimC [His]

E. coli Eco [His]

E. coli DNA Ligase

E. coli Exonuclease I