The microorganism Escherichia coli is usually used for recombinant protein manufacturing. Regardless of a number of advantageous traits like quick development and excessive protein yields, its incapability to simply secrete recombinant proteins into the extracellular medium stays a disadvantage for industrial manufacturing processes. To beat this limitation, a large number of approaches to reinforce the extracellular yield and the secretion effectivity of recombinant proteins have been developed in recent times.
Right here, a complete overview of secretion mechanisms for recombinant proteins from E. coli is given and divided into three fundamental sections. First, the construction of the E. coli cell envelope and the identified pure secretion programs are described. Second, the use and optimization of various one- or two-step secretion programs for recombinant protein manufacturing, in addition to additional permeabilization strategies are mentioned.
Lastly, the often-overlooked position of cell lysis in secretion research and its evaluation are addressed. Thus far, efficient approaches for rising the extracellular protein focus to greater than 10 g/L and virtually 100% secretion effectivity exist, nevertheless, the big vary of optimization strategies and their combos means that the potential for secretory protein manufacturing from E. coli has not but been totally realized.
Scalable Excessive-Efficiency Manufacturing of Recombinant Horseradish Peroxidase from E. coli Inclusion Our bodies
Horseradish peroxidase (HRP), an enzyme omnipresent in biotechnology, remains to be produced from furry root cultures, though this process is time-consuming and solely offers low yields. As well as, the plant-derived enzyme preparation consists of a variable combination of isoenzymes with excessive batch-to-batch variation stopping its use in therapeutic functions. On this research, we current a novel and scalable recombinant HRP manufacturing course of in Escherichia coli that yields a extremely pure, energetic and homogeneous single isoenzyme.
We efficiently developed a multi-step inclusion physique course of giving a last yield of 960 mg energetic HRP/L tradition medium with a purity of ≥99% decided by size-exclusion high-performance liquid chromatography (SEC-HPLC). The Reinheitszahl, in addition to the exercise with 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) and three,3′,5,5′-tetramethylbenzidine (TMB) as lowering substrates, are akin to commercially out there plant HRP. Thus, our preparation of recombinant, unglycosylated HRP from E. coli is a viable various to the enzyme from plant and extremely attention-grabbing for therapeutic functions.
Efficient Allitol Bioproduction from D-Fructose Catalyzed by Recombinant E. coli Complete Cells, and the Situation Optimization, Product Purification
Allitol is a sort of uncommon sugar alcohol with potential utility worth. An engineered pressure, which concurrently expressed D-psicose-3-epimerase (DPE), ribitol dehydrogenase (RDH), and formate dehydrogenase (FDH) three enzymes, was constructed by cloning above three genes into one plasmid and remodeled into the host E. coli pressure, and used because the whole-cell catalysts for biotransformation of allitol from the low-cost substrate of D-fructose. The entire cell allitol biotransformation circumstances have been optimized.
The medium, recombinant gene induction circumstances, and the substrate feeding fee for cultivation of the catalytic cells have been optimized. Then, the fed-batch tradition was made and scaled as much as 10 L fermentor. Lastly, 63.44 g/L allitol was obtained from 100 g/L D-fructose after Three h of biotransformation, and the allitol crystals of 99.9% purity have been obtained through the use of cooling recrystallization. The allitol manufacturing methodology developed on this analysis has excessive product purity, and is very environment friendly, simply scaled up, and appropriate for large-scale manufacturing of extremely purified allitol.
Scalable, two-stage, autoinduction of recombinant protein expression in E. coli using phosphate depletion
We report the scalable manufacturing of recombinant proteins in Escherichia coli, reliant on tightly managed autoinduction, triggered by phosphate depletion within the stationary section. The strategy, reliant on engineered strains and plasmids, allows improved protein expression throughout scales. Expression ranges utilizing this strategy have reached as excessive as 55% of the full mobile protein.
The preliminary use of the tactic in instrumented fed-batch fermentations allows cell densities of ∼30 gCDW/L and protein titers as much as 8.1 ± 0.7 g/L (∼270 mg/gCDW). The method has additionally been tailored to an optimized autoinduction media, enabling routine batch manufacturing at tradition volumes of 20 μl (384-well plates), 100 μl (96-well plates), 20 ml, and 100 ml. In batch cultures, cell densities routinely attain ∼5-7 gCDW/L, providing protein titers above 2 g/L. The methodology has been validated with a set of numerous heterologous proteins and is of basic use for the facile optimization of routine protein expression from excessive throughput screens to fed-batch fermentation.
A simplified methodology for producing laboratory grade recombinant TEV protease from E. coli.
The tobacco etch virus (TEV) protease has develop into a preferred selection for cleaving fusion proteins due to its excessive stringency in sequence recognition. Procedures for isolating recombinant protein from the cytoplasm of E. coli require rupturing of the cell wall by way of enzymatic therapy mixed with sonication or French press.
 E. coli RuvC protein |
|
01-011 |
Sceti |
50 ug |
EUR 479 |
|
Description: The E. coli RuvC protein is available in Europe and for worldwide shipping via Gentaur. |
E. coli RuvC protein |
|
01-012 |
Sceti |
250 ug |
EUR 1184 |
|
Description: The E. coli RuvC protein is available in Europe and for worldwide shipping via Gentaur. |
 RecA Protein E. coli |
|
M1231-200 |
Biovision |
|
EUR 202 |
 Antibody) Escherichia coli (E. coli) Antibody |
|
abx411320-1ml |
Abbexa |
1 ml |
EUR 509 |
|
|
Escherichia coli (E. coli) Antibody |
|
abx415692-01mg |
Abbexa |
0.1 mg |
EUR 634 |
|
|
Escherichia coli (E. coli) Antibody |
|
abx415712-1ml |
Abbexa |
1 ml |
EUR 606 |
|
|
 E. coli Antibody |
|
abx021669-1mg |
Abbexa |
1 mg |
EUR 1295 |
|
|
E. coli Antibody |
|
abx021670-1mg |
Abbexa |
1 mg |
EUR 871 |
|
|
E. coli Antibody |
|
abx021671-02ml |
Abbexa |
0.2 ml |
EUR 495 |
|
|
E. coli Antibody |
|
abx021672-1mg |
Abbexa |
1 mg |
EUR 871 |
|
|
E. coli Antibody |
|
abx021673-1mg |
Abbexa |
1 mg |
EUR 871 |
|
|
E. coli Antibody |
|
abx022970-1ml |
Abbexa |
1 ml |
EUR 732 |
|
|
E. coli Antibody |
|
abx022971-1ml |
Abbexa |
1 ml |
EUR 968 |
|
|
E. coli Antibody |
|
abx022975-1ml |
Abbexa |
1 ml |
EUR 599 |
|
|
E. coli Antibody |
|
abx022977-1ml |
Abbexa |
1 ml |
EUR 676 |
|
|
 E. coli antibody |
|
20-ER13 |
Fitzgerald |
1 ml |
EUR 355 |
|
Description: Rabbit polyclonal E. coli antibody |
E. coli antibody |
|
20C-CR1244GP |
Fitzgerald |
1 ml |
EUR 468 |
|
Description: Goat polyclonal E. coli antibody |
E. coli antibody |
|
10-E09C |
Fitzgerald |
100 ug |
EUR 133 |
|
Description: Mouse monoclonal E. coli antibody |
E. coli antibody |
|
10-E10A |
Fitzgerald |
500 ug |
EUR 493 |
|
Description: Mouse monoclonal E. coli antibody |
E. coli antibody |
|
10-E10B |
Fitzgerald |
500 ug |
EUR 493 |
|
Description: Mouse monoclonal E. coli antibody |
E. coli antibody |
|
10C-CR1244M1 |
Fitzgerald |
100 ug |
EUR 408 |
|
Description: Mouse monoclonal E. coli antibody |
 Antibody (Biotin)) Escherichia coli (E. coli) Antibody (Biotin) |
|
abx411321-1ml |
Abbexa |
1 ml |
EUR 592 |
|
|
 Antibody (FITC)) Escherichia coli (E. coli) Antibody (FITC) |
|
abx411322-1ml |
Abbexa |
1 ml |
EUR 509 |
|
|
Escherichia coli (E. coli) Antibody (Biotin) |
|
abx415713-1ml |
Abbexa |
1 ml |
EUR 718 |
|
|
 E. Coli O 157 Protein |
|
abx061475-01ml |
Abbexa |
0.1 ml |
EUR 551 |
|
|
 (E. coli) [His]) Recombinant Trypanosoma Cruzi Shed-acute-phase-antigen Protein (aa 61-460) (E. coli) [His] |
|
VAng-Wyb8480-100g |
Creative Biolabs |
100 µg |
EUR 3335 |
|
Description: Trypanosoma Cruzi Shed-acute-phase-antigen protein, recombinant protein. |
Recombinant Trypanosoma Cruzi Shed-acute-phase-antigen Protein (aa 61-460) (E. coli) [His] |
|
VAng-Wyb8480-500g |
Creative Biolabs |
500 µg |
EUR 8162 |
|
Description: Trypanosoma Cruzi Shed-acute-phase-antigen protein, recombinant protein. |
 Protein) E. coli Protein GrpE (GrpE) Protein |
|
20-abx261718 |
Abbexa |
-
EUR 3530.00
-
EUR 328.00
-
EUR 230.00
|
|
|
|
 E. coli LexA repressor |
|
01-005 |
Sceti |
50 ug |
EUR 479 |
|
Description: The E. coli LexA repressor is available in Europe and for worldwide shipping via Gentaur. |
E. coli LexA repressor |
|
01-006 |
Sceti |
250 ug |
EUR 1184 |
|
Description: The E. coli LexA repressor is available in Europe and for worldwide shipping via Gentaur. |
 E. coli GroEL Antibody |
|
20012-05011 |
AssayPro |
150 ug |
EUR 217 |
 E. coli CysH Antibody |
|
30281-05111 |
AssayPro |
150 ug |
EUR 261 |
 E. coli DnaJ Antibody |
|
32022-05111 |
AssayPro |
150 ug |
EUR 261 |
 E. coli pykF Antibody |
|
32163-05111 |
AssayPro |
150 ug |
EUR 261 |
 E. coli GOR Antibody |
|
32540-05111 |
AssayPro |
150 ug |
EUR 261 |
 E. coli NDK Antibody |
|
32662-05111 |
AssayPro |
150 ug |
EUR 261 |
 E. coli PPA Antibody |
|
32678-05111 |
AssayPro |
150 ug |
EUR 261 |
 E. coli glk Antibody |
|
32864-05111 |
AssayPro |
150 ug |
EUR 261 |
 E. Coli ldhA Antibody |
|
32898-05111 |
AssayPro |
150 ug |
EUR 261 |
 E. coli deoC Antibody |
|
32936-05111 |
AssayPro |
150 ug |
EUR 261 |
 E. coli Skp Antibody |
|
32971-05111 |
AssayPro |
150 ug |
EUR 261 |
 E. coli nanA Antibody |
|
33092-05111 |
AssayPro |
150 ug |
EUR 261 |
 E. coli O157 Antibody |
|
abx024017-1mg |
Abbexa |
1 mg |
EUR 1010 |
|
|
E. coli O157 Antibody |
|
abx021684-1mg |
Abbexa |
1 mg |
EUR 1017 |
|
|
E. coli O157 Antibody |
|
abx021685-1mg |
Abbexa |
1 mg |
EUR 996 |
|
|
E. coli O157 Antibody |
|
abx021686-1mg |
Abbexa |
1 mg |
EUR 996 |
|
|
E. coli O157 Antibody |
|
abx021687-1mg |
Abbexa |
1 mg |
EUR 996 |
|
|
E. coli O157 Antibody |
|
abx021688-1mg |
Abbexa |
1 mg |
EUR 940 |
|
|
E. coli O157 Antibody |
|
abx021689-1mg |
Abbexa |
1 mg |
EUR 996 |
|
|
) E. coli Antibody (HRP) |
|
abx022972-1ml |
Abbexa |
1 ml |
EUR 599 |
|
|
) E. coli Antibody (FITC) |
|
abx022974-1ml |
Abbexa |
1 ml |
EUR 648 |
|
|
) E. coli Antibody (Biotin) |
|
abx022976-1ml |
Abbexa |
1 ml |
EUR 599 |
|
|
E. coli Antibody (HRP) |
|
abx022978-1ml |
Abbexa |
1 ml |
EUR 878 |
|
|
E. coli Antibody (Biotin) |
|
abx022979-1ml |
Abbexa |
1 ml |
EUR 739 |
|
|
 E. coli O 157 |
|
abx291002-100mg |
Abbexa |
100 mg |
EUR 620 |
|
|
 E. coli Topoisomerase IV |
|
TG2000EC-1 |
TopoGen |
250 units |
EUR 448 |
E. coli Topoisomerase IV |
|
TG2000EC-3 |
TopoGen |
500 units |
EUR 683 |
E. coli Topoisomerase IV |
|
TG2000EC-5 |
TopoGen |
1000 units |
EUR 1052 |
 E. coli DNA Gyrase |
|
TG2000G-1 |
TopoGen |
100 units |
EUR 324 |
E. coli DNA Gyrase |
|
TG2000G-3 |
TopoGen |
500 units |
EUR 705 |
E. coli DNA Gyrase |
|
TG2000G-5 |
TopoGen |
1000 units |
EUR 1164 |
E. coli DNA Gyrase |
|
TG2000G-7 |
TopoGen |
2000 units |
EUR 1836 |
) Enterotoxin STp (E. coli) |
|
H-6248.0500 |
Bachem |
0.5mg |
EUR 696 |
|
Description: Sum Formula: C81H110N20O26S6; CAS# [115474-04-9] net |
Enterotoxin STp (E. coli) |
|
H-6248.1000 |
Bachem |
1.0mg |
EUR 1180 |
|
Description: Sum Formula: C81H110N20O26S6; CAS# [115474-04-9] net |
) E. coli antibody (FITC) |
|
60-E13 |
Fitzgerald |
1 ml |
EUR 378 |
|
Description: Rabbit polyclonal E. coli antibody (FITC) conjugated |
) E. coli antibody (biotin) |
|
60-E13B |
Fitzgerald |
1 ml |
EUR 468 |
|
Description: Rabbit polyclonal E. coli antibody (biotin) conjugated |
) E. coli antibody (HRP) |
|
60-E14 |
Fitzgerald |
1 ml |
EUR 408 |
|
Description: Rabbit polyclonal E. coli antibody (HRP) conjugated |
 E. coli O157 antibody |
|
10-1490 |
Fitzgerald |
100 ug |
EUR 136 |
|
Description: Mouse monoclonal E. coli O157 antibody |
E. coli O157 antibody |
|
10-1521 |
Fitzgerald |
100 ug |
EUR 142 |
|
Description: Mouse monoclonal E. coli O157 antibody |
E. coli O157 antibody |
|
10-E12A |
Fitzgerald |
100 ug |
EUR 385 |
|
Description: Mouse monoclonal E. coli O157 antibody |
E. coli O157 antibody |
|
10C-CR1295M3 |
Fitzgerald |
100 ug |
EUR 392 |
|
Description: Mouse monoclonal E. coli O157 antibody |
 E. Coli HCP antibody |
|
20R-EG001 |
Fitzgerald |
1 ml |
EUR 837 |
|
Description: Goat polyclonal E. Coli HCP antibody |
 E. coli DNA Ligase |
|
M1217-100 |
Biovision |
|
EUR 321 |
 E. coli Exonuclease I |
|
M1222-100 |
Biovision |
|
EUR 251 |
 E. coli Exonuclease III |
|
M1223-100 |
Biovision |
|
EUR 267 |
 E. coli Recombinant IDNK |
|
P1050-10 |
Biovision |
|
EUR 164 |
E. coli Recombinant IDNK |
|
P1050-50 |
Biovision |
|
EUR 381 |
 Recombinant E. coli, Glucokinase |
|
P1054-10 |
Biovision |
|
EUR 164 |
Recombinant E. coli, Glucokinase |
|
P1054-50 |
Biovision |
|
EUR 381 |
 E. Coli Recombinant TRXB |
|
6331-100 |
Biovision |
|
EUR 425 |
 E. Coli Recombinant SlyD |
|
6366-100 |
Biovision |
|
EUR 457 |
 Benzonase, E. Coli Recombinant |
|
7680-25 |
Biovision |
|
EUR 332 |
Benzonase, E. Coli Recombinant |
|
7680-5 |
Biovision |
|
EUR 137 |
 TesA, E. coli esterase |
|
0306-1 |
AthenaES |
100 Units |
EUR 421 |
TesA, E. coli esterase |
|
0306-5 |
AthenaES |
500 Units |
EUR 1705 |
 ELISA Kit) Human Escherichia coli (E. coli) ELISA Kit |
|
abx052493-96tests |
Abbexa |
96 tests |
EUR 668 |
|
|
 ELISA Kit) Cow Escherichia coli (E. coli) ELISA Kit |
|
abx055785-96tests |
Abbexa |
96 tests |
EUR 668 |
|
|
 E. Coli Proteins-Agarose affinity gel for removing E. coli antibodies |
|
EC11-G |
Alpha Diagnostics |
1 ml |
EUR 286 |
) Recombinant Trypanosoma Cruiz Protein (E. coli) |
|
VAng-Wyb8373-1mg |
Creative Biolabs |
1 mg |
EUR 3693 |
|
Description: Trypanosoma Cruiz protein, recombinant protein. |
) Recombinant Human RAGE Protein (E. coli) |
|
VAng-2906Lsx-1mg |
Creative Biolabs |
1 mg |
EUR 3597 |
|
Description: Recombinant Human RAGE (AGER) protein is expressed in E. coli. (Uniprot ID: Q15109) |
Recombinant Human RAGE Protein (E. coli) |
|
VAng-2906Lsx-200g |
Creative Biolabs |
200 µg |
EUR 1329 |
|
Description: Recombinant Human RAGE (AGER) protein is expressed in E. coli. (Uniprot ID: Q15109) |
Recombinant Human RAGE Protein (E. coli) |
|
VAng-2906Lsx-500g |
Creative Biolabs |
500 µg |
EUR 2291 |
|
Description: Recombinant Human RAGE (AGER) protein is expressed in E. coli. (Uniprot ID: Q15109) |
 Protein) E. coli Syndecan 1 (SDC1) Protein |
|
20-abx263542 |
Abbexa |
-
EUR 829.00
-
EUR 411.00
-
EUR 537.00
|
|
|
|
 Protein) E. coli Progesterone Receptor (PGR) Protein |
|
20-abx263552 |
Abbexa |
-
EUR 829.00
-
EUR 411.00
-
EUR 537.00
|
|
|
|
 [GST]) Recombinant CMV Protein (E. coli) [GST] |
|
VAng-Lsx0035-inquire |
Creative Biolabs |
inquire |
Ask for price |
|
Description: CMV [GST], recombinant protein from E. coli. |
 E. coli 0157:H7 cell protein |
|
30-AE13 |
Fitzgerald |
1 ml |
EUR 284 |
|
Description: Heat inactivated E. coli O157:H7 cells |
) Recombinant HIV-2 E gp 36 Protein (E. coli) |
|
VAng-0534Lsx-inquire |
Creative Biolabs |
inquire |
Ask for price |
|
Description: HIV type 2 Envelope glycoprotein 36, recombinant protein from E. coli. MW 16 kDa, 1 mg/mL. |
 Protein) E. coli Chaperone Protein Dnaj (DNAJ) Protein |
|
20-abx260479 |
Abbexa |
-
EUR 230.00
-
EUR 2332.00
-
EUR 328.00
|
|
|
|
 Protein) E. coli Chaperone Protein SKP (SKP) Protein |
|
20-abx260554 |
Abbexa |
-
EUR 2861.00
-
EUR 328.00
-
EUR 230.00
|
|
|
|
 Protein) E. coli Green Fluorescent Protein (GFP) Protein |
|
20-abx260632 |
Abbexa |
-
EUR 230.00
-
EUR 3042.00
-
EUR 328.00
|
|
|
|
 (fusion protein) for WB +ve control) Purified Rec. E. Coli Thioredoxin (TrxA, E. coli) (fusion protein) for WB +ve control |
|
THRX11-C |
Alpha Diagnostics |
100 ul |
EUR 286 |
 Antibody) Escherichia coli Serotype O2 (E. coli O2) Antibody |
|
abx411330-02mg |
Abbexa |
0.2 mg |
EUR 565 |
|
|
 Antibody) Escherichia coli Serotype O15 (E. coli O15) Antibody |
|
abx411331-02mg |
Abbexa |
0.2 mg |
EUR 565 |
|
|
 Antibody) Escherichia coli Serotype O20 (E. coli O20) Antibody |
|
abx411332-02mg |
Abbexa |
0.2 mg |
EUR 565 |
|
|
 (E. coli) [His]) Recombinant Trypanosoma Brucei Variant-specific antigen Protein (aa 23-367) (E. coli) [His] |
|
VAng-Wyb8311-100g |
Creative Biolabs |
100 µg |
EUR 3335 |
|
Description: Trypanosoma Brucei brucei Variant-specific antigen protein, recombinant protein. |
Recombinant Trypanosoma Brucei Variant-specific antigen Protein (aa 23-367) (E. coli) [His] |
|
VAng-Wyb8311-500g |
Creative Biolabs |
500 µg |
EUR 8162 |
|
Description: Trypanosoma Brucei brucei Variant-specific antigen protein, recombinant protein. |
 (E. coli)) Recombinant Plasmodium Falciparum GTP-binding nuclear protein Ran Protein (aa 1-214) (E. coli) |
|
VAng-Wyb6469-inquire |
Creative Biolabs |
inquire |
Ask for price |
|
Description: Plasmodium Falciparum GTP-binding nuclear protein Ran, recombinant protein. |
) ELISA kit for Human TPS (Tissue Polypeptide Specific Antigen) |
|
E-EL-H2362 |
Elabscience Biotech |
1 plate of 96 wells |
EUR 534 |
|
|
|
Description: A sandwich ELISA kit for quantitative measurement of Human TPS (Tissue Polypeptide Specific Antigen) in samples from Serum, Plasma, Cell supernatant |
) CLIA kit for Human TPS (Tissue Polypeptide Specific Antigen) |
|
E-CL-H1374 |
Elabscience Biotech |
1 plate of 96 wells |
EUR 584 |
|
|
|
Description: A sandwich CLIA kit for quantitative measurement of Human TPS (Tissue Polypeptide Specific Antigen) in samples from Serum, Plasma, Cell supernatant |
) Recombinant Diacylglycerol Kinase (E. coli) |
|
MET-5035 |
Cell Biolabs |
100 mg |
EUR 276 |
|
Description: Purified recombinant prokaryotic DAG kinase expressed in E. coli |
Recombinant Diacylglycerol Kinase (E. coli) |
|
MET-5035-5 |
Cell Biolabs |
5 x 100 mg |
EUR 920 |
|
Description: Purified recombinant prokaryotic DAG kinase expressed in E. coli |
 E. coli RecQ DNA helicase |
|
01-003 |
Sceti |
50 ug |
EUR 479 |
|
Description: The E. coli RecQ DNA helicase is available in Europe and for worldwide shipping via Gentaur. |
E. coli RecQ DNA helicase |
|
01-004 |
Sceti |
250? g |
EUR 1549 |
|
Description: The E. coli RecQ DNA helicase is available in Europe and for worldwide shipping via Gentaur. |
 E. coli Glycerol Kinase Antibody |
|
32699-05111 |
AssayPro |
150 ug |
EUR 261 |
 Antibody) E. coli Tryptophanase (Tnase) Antibody |
|
33517-05111 |
AssayPro |
150 ug |
EUR 261 |
 Mouse antibody for E. coli |
|
1063 |
Virostat |
100 ug |
EUR 365.38 |
|
Description: This is Mouse monoclonal FITC conjugated antibody against E coli O157 for WB, ELISA. |
 Antibody) E. coli O157 (9.88) Antibody |
|
BNC040207-100 |
Biotium |
100uL |
EUR 199 |
|
Description: Primary antibody against E. coli O157 (9.88),CF405S conjugate, Concentration: 0.1mg/mL |
E. coli O157 (9.88) Antibody |
|
BNC040207-500 |
Biotium |
500uL |
EUR 544 |
|
Description: Primary antibody against E. coli O157 (9.88),CF405S conjugate, Concentration: 0.1mg/mL |
E. coli O157 (9.88) Antibody |
|
BNC470207-100 |
Biotium |
100uL |
EUR 199 |
|
Description: Primary antibody against E. coli O157 (9.88),CF647 conjugate, Concentration: 0.1mg/mL |
E. coli O157 (9.88) Antibody |
|
BNC470207-500 |
Biotium |
500uL |
EUR 544 |
|
Description: Primary antibody against E. coli O157 (9.88),CF647 conjugate, Concentration: 0.1mg/mL |
E. coli O157 (9.88) Antibody |
|
BNC550207-100 |
Biotium |
100uL |
EUR 199 |
|
Description: Primary antibody against E. coli O157 (9.88),CF555 conjugate, Concentration: 0.1mg/mL |
E. coli O157 (9.88) Antibody |
|
BNC550207-500 |
Biotium |
500uL |
EUR 544 |
|
Description: Primary antibody against E. coli O157 (9.88),CF555 conjugate, Concentration: 0.1mg/mL |
E. coli O157 (9.88) Antibody |
|
BNCB0207-100 |
Biotium |
100uL |
EUR 199 |
|
Description: Primary antibody against E. coli O157 (9.88),Biotin conjugate, Concentration: 0.1mg/mL |
E. coli O157 (9.88) Antibody |
|
BNCB0207-500 |
Biotium |
500uL |
EUR 544 |
|
Description: Primary antibody against E. coli O157 (9.88),Biotin conjugate, Concentration: 0.1mg/mL |
E. coli O157 (9.88) Antibody |
|
BNC810207-100 |
Biotium |
100uL |
EUR 199 |
|
Description: Primary antibody against E. coli O157 (9.88),CF680R conjugate, Concentration: 0.1mg/mL |
E. coli O157 (9.88) Antibody |
|
BNC810207-500 |
Biotium |
500uL |
EUR 544 |
|
Description: Primary antibody against E. coli O157 (9.88),CF680R conjugate, Concentration: 0.1mg/mL |
 E. coli Enterotoxin B Antibody |
|
abx021678-1mg |
Abbexa |
1 mg |
EUR 599 |
|
|
E. coli Enterotoxin B Antibody |
|
abx021679-1mg |
Abbexa |
1 mg |
EUR 606 |
|
|
E. coli Enterotoxin B Antibody |
|
abx021680-1mg |
Abbexa |
1 mg |
EUR 599 |
|
|
) E. coli O157 Antibody (FITC) |
|
abx021683-1mg |
Abbexa |
1 mg |
EUR 2332 |
|
|
 E. coli O157:H7 Antibody |
|
abx022980-1mg |
Abbexa |
1 mg |
EUR 599 |
|
|
 E. coli Enterotoxin LT Antibody |
|
abx023834-1mg |
Abbexa |
1 mg |
EUR 732 |
|
|
E. coli Enterotoxin LT Antibody |
|
abx023835-1mg |
Abbexa |
1 mg |
EUR 732 |
|
|
E. coli Enterotoxin LT Antibody |
|
abx023836-1mg |
Abbexa |
1 mg |
EUR 732 |
|
|
E. coli Enterotoxin LT Antibody |
|
abx023837-1mg |
Abbexa |
1 mg |
EUR 732 |
|
|
 E. coli Enterotoxin STp Antibody |
|
abx023838-1mg |
Abbexa |
1 mg |
EUR 878 |
|
|
E. coli Enterotoxin STp Antibody |
|
abx023839-1mg |
Abbexa |
1 mg |
EUR 878 |
|
|
E. coli Enterotoxin STp Antibody |
|
abx023840-1mg |
Abbexa |
1 mg |
EUR 878 |
|
|
E. coli O157 (9.88) Antibody |
|
BNC700207-100 |
Biotium |
100uL |
EUR 199 |
|
Description: Primary antibody against E. coli O157 (9.88),CF770 conjugate, Concentration: 0.1mg/mL |
E. coli O157 (9.88) Antibody |
|
BNC700207-500 |
Biotium |
500uL |
EUR 544 |
|
Description: Primary antibody against E. coli O157 (9.88),CF770 conjugate, Concentration: 0.1mg/mL |
E. coli O157 (9.88) Antibody |
|
BNC880207-100 |
Biotium |
100uL |
EUR 199 |
|
Description: Primary antibody against E. coli O157 (9.88),CF488A conjugate, Concentration: 0.1mg/mL |
E. coli O157 (9.88) Antibody |
|
BNC880207-500 |
Biotium |
500uL |
EUR 544 |
|
Description: Primary antibody against E. coli O157 (9.88),CF488A conjugate, Concentration: 0.1mg/mL |
E. coli O157 (9.88) Antibody |
|
BNC940207-100 |
Biotium |
100uL |
EUR 199 |
|
Description: Primary antibody against E. coli O157 (9.88),CF594 conjugate, Concentration: 0.1mg/mL |
E. coli O157 (9.88) Antibody |
|
BNC940207-500 |
Biotium |
500uL |
EUR 544 |
|
Description: Primary antibody against E. coli O157 (9.88),CF594 conjugate, Concentration: 0.1mg/mL |
E. coli O157 (9.88) Antibody |
|
BNC680207-100 |
Biotium |
100uL |
EUR 199 |
|
Description: Primary antibody against E. coli O157 (9.88),CF568 conjugate, Concentration: 0.1mg/mL |
E. coli O157 (9.88) Antibody |
|
BNC680207-500 |
Biotium |
500uL |
EUR 544 |
|
Description: Primary antibody against E. coli O157 (9.88),CF568 conjugate, Concentration: 0.1mg/mL |
E. coli O157 (9.88) Antibody |
|
BNCA0207-250 |
Biotium |
250uL |
EUR 383 |
|
Description: Primary antibody against E. coli O157 (9.88),APC conjugate, Concentration: 0.1mg/mL |
E. coli O157 (9.88) Antibody |
|
BNCAP0207-100 |
Biotium |
100uL |
EUR 199 |
|
Description: Primary antibody against E. coli O157 (9.88),Alkaline Phosphatase conjugate, Concentration: 0.1mg/mL |
E. coli O157 (9.88) Antibody |
|
BNCAP0207-500 |
Biotium |
500uL |
EUR 544 |
|
Description: Primary antibody against E. coli O157 (9.88),Alkaline Phosphatase conjugate, Concentration: 0.1mg/mL |
E. coli O157 (9.88) Antibody |
|
BNUM0207-50 |
Biotium |
50uL |
EUR 395 |
|
Description: Primary antibody against E. coli O157 (9.88), 1mg/mL |
E. coli O157 (9.88) Antibody |
|
BNCP0207-250 |
Biotium |
250uL |
EUR 383 |
|
Description: Primary antibody against E. coli O157 (9.88),PerCP conjugate, Concentration: 0.1mg/mL |
E. coli O157 (9.88) Antibody |
|
BNC050207-100 |
Biotium |
100uL |
EUR 199 |
|
Description: Primary antibody against E. coli O157 (9.88),CF405M conjugate, Concentration: 0.1mg/mL |
E. coli O157 (9.88) Antibody |
|
BNC050207-500 |
Biotium |
500uL |
EUR 544 |
|
Description: Primary antibody against E. coli O157 (9.88),CF405M conjugate, Concentration: 0.1mg/mL |
E. coli O157 (9.88) Antibody |
|
BNC400207-100 |
Biotium |
100uL |
EUR 199 |
|
Description: Primary antibody against E. coli O157 (9.88),CF640R conjugate, Concentration: 0.1mg/mL |
E. coli O157 (9.88) Antibody |
|
BNC400207-500 |
Biotium |
500uL |
EUR 544 |
|
Description: Primary antibody against E. coli O157 (9.88),CF640R conjugate, Concentration: 0.1mg/mL |
E. coli O157 (9.88) Antibody |
|
BNCR0207-250 |
Biotium |
250uL |
EUR 383 |
|
Description: Primary antibody against E. coli O157 (9.88),RPE conjugate, Concentration: 0.1mg/mL |
E. coli O157 (9.88) Antibody |
|
BNUB0207-100 |
Biotium |
100uL |
EUR 209 |
|
Description: Primary antibody against E. coli O157 (9.88), Concentration: 0.2mg/mL |
E. coli O157 (9.88) Antibody |
|
BNUB0207-500 |
Biotium |
500uL |
EUR 458 |
|
Description: Primary antibody against E. coli O157 (9.88), Concentration: 0.2mg/mL |
E. coli O157 (9.88) Antibody |
|
BNC430207-100 |
Biotium |
100uL |
EUR 199 |
|
Description: Primary antibody against E. coli O157 (9.88),CF543 conjugate, Concentration: 0.1mg/mL |
E. coli O157 (9.88) Antibody |
|
BNC430207-500 |
Biotium |
500uL |
EUR 544 |
|
Description: Primary antibody against E. coli O157 (9.88),CF543 conjugate, Concentration: 0.1mg/mL |
E. coli O157 (9.88) Antibody |
|
BNCH0207-100 |
Biotium |
100uL |
EUR 199 |
|
Description: Primary antibody against E. coli O157 (9.88),Horseradish Peroxidase conjugate, Concentration: 0.1mg/mL |
E. coli O157 (9.88) Antibody |
|
BNCH0207-500 |
Biotium |
500uL |
EUR 544 |
|
Description: Primary antibody against E. coli O157 (9.88),Horseradish Peroxidase conjugate, Concentration: 0.1mg/mL |
E. coli O157 (9.88) Antibody |
|
BNC610207-100 |
Biotium |
100uL |
EUR 199 |
|
Description: Primary antibody against E. coli O157 (9.88),CF660R conjugate, Concentration: 0.1mg/mL |
E. coli O157 (9.88) Antibody |
|
BNC610207-500 |
Biotium |
500uL |
EUR 544 |
|
Description: Primary antibody against E. coli O157 (9.88),CF660R conjugate, Concentration: 0.1mg/mL |
E. coli O157 (9.88) Antibody |
|
BNC800207-100 |
Biotium |
100uL |
EUR 199 |
|
Description: Primary antibody against E. coli O157 (9.88),CF680 conjugate, Concentration: 0.1mg/mL |
E. coli O157 (9.88) Antibody |
|
BNC800207-500 |
Biotium |
500uL |
EUR 544 |
|
Description: Primary antibody against E. coli O157 (9.88),CF680 conjugate, Concentration: 0.1mg/mL |
) rHu BMP-2 (E. coli) |
|
AK8356-0100 |
Akron Biotech |
100µg |
Ask for price |
) E. coli O157 antibody (FITC) |
|
61C-CR1295M1F |
Fitzgerald |
100 ug |
EUR 408 |
|
Description: Mouse monoclonal E. coli O157 antibody (FITC) |
E. coli O157 antibody (FITC) |
|
61C-CR1295M4F |
Fitzgerald |
100 ug |
EUR 468 |
|
Description: Mouse monoclonal E. coli O157 antibody (FITC) |
 E. coli O157:H7 antibody |
|
70-XG13 |
Fitzgerald |
1 mg |
EUR 612 |
|
Description: Affinity purified Goat polyclonal E. coli O157:H7 antibody. |
) E. coli antibody (K99 Pilus) |
|
10-E20A |
Fitzgerald |
500 ug |
EUR 493 |
|
Description: Mouse monoclonal E. coli antibody (K99 Pilus) |
 E. coli DNA Polymerase I |
|
M1210-100 |
Biovision |
|
EUR 256 |
 E. coli Recombinant Carbonic anhydrase |
|
P1049-10 |
Biovision |
|
EUR 164 |
E. coli Recombinant Carbonic anhydrase |
|
P1049-50 |
Biovision |
|
EUR 381 |
) Insulin, human recombinant (E. coli) |
|
4772-25 |
Biovision |
|
EUR 452 |
Insulin, human recombinant (E. coli) |
|
4772-5 |
Biovision |
|
EUR 207 |
 E. Coli Recombinant Thioredoxin 1 |
|
6329-50 |
Biovision |
|
EUR 403 |
 Oligomycin E |
|
O021-1MG |
TOKU-E |
1 mg |
EUR 318 |
Oligomycin E |
|
O021-5MG |
TOKU-E |
5 mg |
EUR 1040 |
 Paraherquamide E |
|
P070-0.5MG |
TOKU-E |
0.5 mg |
EUR 400 |
Paraherquamide E |
|
P070-2.5MG |
TOKU-E |
2.5 mg |
EUR 1325 |
 Phenelfamycin E |
|
P078-1MG |
TOKU-E |
1 mg |
EUR 318 |
Phenelfamycin E |
|
P078-5MG |
TOKU-E |
5 mg |
EUR 1040 |
 Roquefortine E |
|
R035-1MG |
TOKU-E |
1 mg |
EUR 224 |
Roquefortine E |
|
R035-5MG |
TOKU-E |
5 mg |
EUR 708 |
 Deoxybrevianamide E |
|
D083-1MG |
TOKU-E |
1 mg |
EUR 278 |
Deoxybrevianamide E |
|
D083-5MG |
TOKU-E |
5 mg |
EUR 897 |
 Cytochalasin E |
|
C197-1MG |
TOKU-E |
1 mg |
EUR 131 |
Cytochalasin E |
|
C197-5MG |
TOKU-E |
5 mg |
EUR 385 |
 Mouse Anti-E. coli and LPS IgG1 Monoclonal Antibody, Clone 1C6 |
|
7098 |
Chondrex |
1 mg/ml x 0.1 ml |
EUR 482.1 |
|
Description: Mouse Anti-E. coli and LPS IgG1 Monoclonal Antibody |
 Mouse Anti-E. coli and LPS IgG2a Monoclonal Antibody, Clone 1E1 |
|
7099 |
Chondrex |
1 mg/ml x 0.1 ml |
EUR 482.1 |
|
Description: Mouse Anti-E. coli and LPS IgG2a Monoclonal Antibody |
 Mouse Anti-E. coli and LPS IgG2b Monoclonal Antibody, Clone 2H10 |
|
7100 |
Chondrex |
1 mg/ml x 0.1 ml |
EUR 482.1 |
|
Description: Mouse Anti-E. coli and LPS IgG2b Monoclonal Antibody |
 GlpK Glycerol kinase E. Coli Recombinant Protein |
|
PROTP0A6F3 |
BosterBio |
Regular: 20ug |
EUR 317 |
|
Description: GlpK E. Coli Recombinant produced in E. coli is a single, non-glycosylated polypeptide chain containing 525 amino acids (1-502 a.a) and having a molecular mass of 58.6 kDa.;GlpK is fused to a 23 amino acid His-tag at N-terminus& purified by proprietary chromatographic techniques. |
) Recombinant Plasmodium Berghei CG10 Protein (E. coli) |
|
VAng-Wyb7595-inquire |
Creative Biolabs |
inquire |
Ask for price |
|
Description: Plasmodium Berghei Putative chloroquine resistance transporter protein, partial, recombinant protein. |
) Recombinant Plasmodium Berghei COI Protein (E. coli) |
|
VAng-Wyb7599-inquire |
Creative Biolabs |
inquire |
Ask for price |
|
Description: Plasmodium Berghei Cytochrome c oxidase subunit 1 protein, partial, recombinant protein. |
) Recombinant Plasmodium Berghei CPK4 Protein (E. coli) |
|
VAng-Wyb7603-inquire |
Creative Biolabs |
inquire |
Ask for price |
|
Description: Plasmodium Berghei (strain Anka) Calcium-dependent protein kinase 4 protein, partial, recombinant protein. |
) Recombinant Plasmodium Berghei CS Protein (E. coli) |
|
VAng-Wyb7615-inquire |
Creative Biolabs |
inquire |
Ask for price |
|
Description: Plasmodium Berghei (strain Anka) Circumsporozoite protein, partial, recombinant protein. |
) Recombinant Plasmodium Berghei PB000816.02.0 Protein (E. coli) |
|
VAng-Wyb7659-inquire |
Creative Biolabs |
inquire |
Ask for price |
|
Description: Plasmodium Berghei (strain Anka) tRNA-splicing ligase RtcB homolog protein, partial, recombinant protein. |
) Recombinant Plasmodium Chabaudi CG10 Protein (E. coli) |
|
VAng-Wyb7707-inquire |
Creative Biolabs |
inquire |
Ask for price |
|
Description: Plasmodium Chabaudi Putative chloroquine resistance transporter protein, partial, recombinant protein. |
) Recombinant Plasmodium Chabaudi COI Protein (E. coli) |
|
VAng-Wyb7711-inquire |
Creative Biolabs |
inquire |
Ask for price |
|
Description: Plasmodium Chabaudi Cytochrome c oxidase subunit 1 protein, partial, recombinant protein. |
) Recombinant Plasmodium Chabaudi PC000176.04.0 Protein (E. coli) |
|
VAng-Wyb7735-inquire |
Creative Biolabs |
inquire |
Ask for price |
|
Description: Plasmodium Chabaudi Ubiquinone biosynthesis protein COQ4 homolog, mitochondrial protein, recombinant protein. |
) Recombinant Plasmodium Chabaudi PC000321.02.0 Protein (E. coli) |
|
VAng-Wyb7739-inquire |
Creative Biolabs |
inquire |
Ask for price |
|
Description: Plasmodium Chabaudi Protein SEY1 homolog, partial, recombinant protein. |
) Recombinant Plasmodium Yoelii CPK1 Protein (E. coli) |
|
VAng-Wyb7894-inquire |
Creative Biolabs |
inquire |
Ask for price |
|
Description: Plasmodium Yoelii Calcium-dependent protein kinase 1 protein, partial, recombinant protein. |
) Recombinant Plasmodium Yoelii CPK3 Protein (E. coli) |
|
VAng-Wyb7898-inquire |
Creative Biolabs |
inquire |
Ask for price |
|
Description: Plasmodium Yoelii Calcium-dependent protein kinase 3 protein, partial, recombinant protein. |
) Recombinant Plasmodium Yoelii CPK4 Protein (E. coli) |
|
VAng-Wyb7902-inquire |
Creative Biolabs |
inquire |
Ask for price |
|
Description: Plasmodium Yoelii Calcium-dependent protein kinase 4 protein, partial, recombinant protein. |
) Recombinant Plasmodium Yoelii PY01232 Protein (E. coli) |
|
VAng-Wyb7946-inquire |
Creative Biolabs |
inquire |
Ask for price |
|
Description: Plasmodium Yoelii Myosin-A protein, partial, recombinant protein. |
) Recombinant Plasmodium Yoelii PY03426 Protein (E. coli) |
|
VAng-Wyb7978-inquire |
Creative Biolabs |
inquire |
Ask for price |
|
Description: Plasmodium Yoelii Translation factor GUF1 homolog, mitochondrial protein, partial, recombinant protein. |
) Recombinant Plasmodium Yoelii PY03776 Protein (E. coli) |
|
VAng-Wyb7982-inquire |
Creative Biolabs |
inquire |
Ask for price |
|
Description: Plasmodium Yoelii tRNA-splicing ligase RtcB homolog protein, partial, recombinant protein. |
) Recombinant Plasmodium Yoelii PY05061 Protein (E. coli) |
|
VAng-Wyb7986-inquire |
Creative Biolabs |
inquire |
Ask for price |
|
Description: Plasmodium Yoelii Putative chloroquine resistance transporter protein, partial, recombinant protein. |
Right here we current an expedited methodology for producing laboratory-grade TEV protease in E. coli utilizing a freeze-thaw methodology, adopted by purification with immobilized metallic affinity chromatography. Protease is obtained by expression from the pDZ2087 plasmid in BL21 (DE3) cells. Proteolysis ensuing from this product, cleaves a maltose-binding protein fusion to completion at a fusion-to-protease molar ratio of 50:1.
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