Screening and Production of Recombinant Human Proteins: Protein Production in E. coli

In Chapter 3 , we described the Structural Genomics Consortium (SGC) course of for producing a number of constructs of truncated variations of every protein utilizing LIC. On this chapter we offer a step-by-step process of our E. coli system for take a look at expressing intracellular (soluble) proteins in a 96-well format that allows us to determine which proteins or truncated variations are expressed in a soluble and secure kind appropriate for structural research. As well as, we element the method for scaling up cultures for large-scale protein purification.

This stage of manufacturing is required to acquire enough portions (i.e., milligram quantities) of protein for additional characterization and/or structural research (e.g., crystallization or cryo-EM experiments). Our normal course of is purification by immobilized steel affinity chromatography (IMAC) utilizing nickel resin adopted by dimension exclusion chromatography (SEC), with further procedures arising from the complexity of the protein itself.

Examine of the results of three.1 THz radiation on the expression of recombinant purple fluorescent protein (RFP) in E. coli

Lately, many research have been performed to research the non-thermal results of THz radiation on completely different organisms, however additional research are wanted to totally elucidate the results, particularly on the molecular stage. On this research, we explored the results of at 3.1 THz radiation on protein expression in Escherichia coli (E. coli) utilizing purple fluorescent protein as a reporter molecule.

After Eight hours of steady THz irradiation of micro organism on LB (Luria-Bertani) stable plates at a mean energy of 33 mW/cm² and 10 Hz pulse repetition frequency, we discovered that the plasmid copy quantity, protein expression and fluorescence depth of micro organism from the irradiated space have been 3.8-, 2.7-, and three.

Three occasions greater than in micro organism from the un-irradiated space, respectively. These findings recommend that plasmid replication modified considerably in micro organism uncovered to three.1 THz radiation, leading to elevated protein expression as evidenced by elevated fluorescence depth of the RFP reporter.

Expression of Recombinant Leptospiral Floor Lipoprotein-Lsa27 in E. coli and Its Evaluation for Serodiagnosis of Bovine Leptospirosis by Latex Agglutination Check

The expressed recombinant leptospiral floor adhesion lipoprotein (Lsa27) of pathogenic Leptospira in E. coli was evaluated for the detection of Leptospira antibodies in cattle sera by latex agglutination take a look at (LAT). The Lsa27 missing sign peptide coding gene sequences from L. interrogans serovar Pomona was amplified (~ 660 bp) by PCR and the amplicon was cloned into pETiteN-HisKan vector.

The expressed recombinant Lsa27 histidine-tagged fusion protein (rLsa27) was Ni-NTA affinity purified below denaturation adopted by renaturation strategies. The purified rLsa27 was characterised by SDS-PAGE and immunoblot, which confirmed the leptospiral protein with a MW of ~ 25 kDa. Additional, the ready sensitized latex beads coated with rLsa27 have been evaluated as a diagnostic antigen for detection of pathogenic Leptospira antibodies by utilizing identified microscopic agglutination take a look at (MAT) constructive (n = 74) and detrimental (n = 62) sera for Leptospira antibodies in LAT, which revealed the relative diagnostic sensitivity of 91.89% and specificity of 87.10% in opposition to the gold normal serological take a look at, MAT.

Moreover, on analysis of developed rLsa27 LAT utilizing serum samples from cattle related to the historical past of abortions and reproductive dysfunction (n = 309), the relative sensitivity of 96.15%, and specificity of 89.11% have been noticed. Due to this fact, this fast subject take a look at utilizing the rLsa27 is first of its form and it could possibly be used as a screening take a look at for the detection of Leptospira antibodies or it may be complemented by different diagnostics for the analysis /surveillance of bovine leptospirosis.

E. coli expression and immunological evaluation of expressed recombinant Newcastle illness virus hemagglutinin-neuraminidase protein in chickens

Yearly, the poultry trade experiences important financial losses as a consequence of epidemics of Newcastle illness virus (NDV). Growing new vaccines by figuring out and utilizing the immunogenic hemagglutinin-neuraminidase (HN) protein can defend the poultry trade. Within the current research, the full-length HN protein was expressed in Escherichia coli (E. coli) BL21 (DE3) cells, purified by way of affinity chromatography and detected by way of western blot evaluation utilizing His-specific antibodies.

The purified HN protein was additional evaluated in chickens to review the immune response in opposition to NDV. The profitable manufacturing of HN-specific IgY proved the exercise of the purified HN protein. IgY was current within the serum of immunized chickens. Nonetheless, the immune response was greater in chickens immunized with purified HN protein together with full and incomplete adjuvants than in chickens immunized with solely the HN protein. Key phrases: protein; Newcastle illness virus; poultry; infectious illnesses; vaccines.

Quick purification of recombinant monomeric amyloid-β from E. coli and amyloid-β-mCherry aggregates from mammalian cells

The Alzheimer’s illness associated peptide, Amyloid-beta (Aβ)1-40 and 1-42, has confirmed tough to be purified as a recombinant monomeric protein due its expression in E. coli resulting in the formation of insoluble inclusion our bodies and its tendency to rapidly kind insoluble aggregates.

An enormous array of strategies have been used to date, but many have pitfalls, resembling using tags for ease of Aβ isolation, the formation of Aβ multimers inside the timeframe of extraction or the necessity to reconstitute Aβ from a freeze dried state.

Tissue Factor Pathway Inhibitor 2 (TFPI2) Polyclonal Antibody (Bovine), Biotinylated
4-PAB940Bo01-Biotin	Cloud-Clone	EUR 400.80 EUR 3331.20 EUR 967.20 EUR 494.40 EUR 273.60	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Bovine Tissue Factor Pathway Inhibitor 2 (TFPI2). This antibody is labeled with Biotin.

Tissue Factor Pathway Inhibitor 2 (TFPI2) Antibody
20-abx103659	Abbexa	EUR 493.20 EUR 159.60 EUR 1378.80 EUR 678.00 EUR 376.80	100 ug 10 ug 1 mg 200 ug 50 ug

Tissue Factor Pathway Inhibitor 2 (TFPI2) Antibody
20-abx103660	Abbexa	EUR 510.00 EUR 159.60 EUR 1412.40 EUR 693.60 EUR 393.60	100 ug 10 ug 1 mg 200 ug 50 ug

Tissue Factor Pathway Inhibitor 2 (TFPI2) Antibody
20-abx103661	Abbexa	EUR 526.80 EUR 159.60 EUR 1496.40 EUR 710.40 EUR 393.60	100 ug 10 ug 1 mg 200 ug 50 ug

Tissue Factor Pathway Inhibitor 2 (TFPI2) Antibody
20-abx103662	Abbexa	EUR 560.40 EUR 159.60 EUR 1612.80 EUR 760.80 EUR 427.20	100 ug 10 ug 1 mg 200 ug 50 ug

Tissue Factor Pathway Inhibitor 2 (TFPI2) Antibody
20-abx174805	Abbexa	EUR 978.00 EUR 510.00	1 mg 200 ug

Tissue Factor Pathway Inhibitor 2 (TFPI2) Antibody
20-abx210903	Abbexa	EUR 493.20 EUR 360.00	100 ul 50 ul

Tissue Factor Pathway Inhibitor 2 (TFPI2) Antibody
20-abx322259	Abbexa	EUR 360.00 EUR 292.80	100 ul 50 ul

Tissue Factor Pathway Inhibitor 2 (TFPI2) Antibody
abx122715-100ug	Abbexa	100 ug	EUR 469.2

Tissue Factor Pathway Inhibitor 2 (TFPI2) Antibody
20-abx323594	Abbexa	EUR 376.80 EUR 292.80	100 ug 50 ug

Tissue Factor Pathway Inhibitor 2 (TFPI2) Antibody
20-abx320289	Abbexa	EUR 360.00 EUR 292.80	100 ul 50 ul

Tissue Factor Pathway Inhibitor 2 (TFPI2) Antibody
20-abx339482	Abbexa	EUR 493.20 EUR 360.00	100 ul 50 ul

Tissue Factor Pathway Inhibitor 2 (TFPI2) Antibody
20-abx006327	Abbexa	EUR 493.20 EUR 710.40 EUR 218.40 EUR 376.80	100 ul 200 ul 20 ul 50 ul

Recombinant Tissue Factor Pathway Inhibitor 2 (TFPI2)
4-RPB940Bo01	Cloud-Clone	EUR 670.50 EUR 303.60 EUR 2184.38 EUR 808.13 EUR 1496.26 EUR 524.40 EUR 5280.96	100 ug 10ug 1 mg 200 ug 500 ug 50ug 5 mg
Description: Recombinant Bovine Tissue Factor Pathway Inhibitor 2 expressed in: E.coli

Recombinant Tissue Factor Pathway Inhibitor 2 (TFPI2)
4-RPB940Hu01	Cloud-Clone	EUR 550.08 EUR 271.20 EUR 1732.80 EUR 657.60 EUR 1195.20 EUR 444.00 EUR 4152.00	100 ug 10ug 1 mg 200 ug 500 ug 50ug 5 mg
Description: Recombinant Human Tissue Factor Pathway Inhibitor 2 expressed in: E.coli

Recombinant Tissue Factor Pathway Inhibitor 2 (TFPI2)
4-RPB940Mu01	Cloud-Clone	EUR 603.84 EUR 285.60 EUR 1934.40 EUR 724.80 EUR 1329.60 EUR 480.00 EUR 4656.00	100 ug 10ug 1 mg 200 ug 500 ug 50ug 5 mg
Description: Recombinant Mouse Tissue Factor Pathway Inhibitor 2 expressed in: E.coli

Recombinant Tissue Factor Pathway Inhibitor 2 (TFPI2)
4-RPB940Ra01	Cloud-Clone	EUR 657.60 EUR 300.00 EUR 2136.00 EUR 792.00 EUR 1464.00 EUR 516.00 EUR 5160.00	100 ug 10ug 1 mg 200 ug 500 ug 50ug 5 mg
Description: Recombinant Rat Tissue Factor Pathway Inhibitor 2 expressed in: E.coli

Rat Tissue Factor Pathway Inhibitor 2 (TFPI2) Protein
20-abx069325	Abbexa	EUR 910.80 EUR 360.00 EUR 2865.60 EUR 1095.60 EUR 644.40	100 ug 10 ug 1 mg 200 ug 50 ug

Cow Tissue Factor Pathway Inhibitor 2 (TFPI2) Protein
20-abx069327	Abbexa	EUR 927.60 EUR 360.00 EUR 2932.80 EUR 1111.20 EUR 661.20	100 ug 10 ug 1 mg 200 ug 50 ug

Human Tissue Factor Pathway Inhibitor 2 (TFPI2) Protein
20-abx069324	Abbexa	EUR 777.60 EUR 326.40 EUR 2331.60 EUR 910.80 EUR 560.40	100 ug 10 ug 1 mg 200 ug 50 ug

Mouse Tissue Factor Pathway Inhibitor 2 (TFPI2) Protein
20-abx069326	Abbexa	EUR 844.80 EUR 343.20 EUR 2598.00 EUR 994.80 EUR 594.00	100 ug 10 ug 1 mg 200 ug 50 ug

Pig Tissue Factor Pathway Inhibitor 2 (TFPI2) ELISA Kit
abx362050-96tests	Abbexa	96 tests	EUR 990

Cow Tissue Factor Pathway Inhibitor 2 (TFPI2) ELISA Kit
abx518897-96tests	Abbexa	96 tests	EUR 1093.2

Human Tissue Factor Pathway Inhibitor 2 (TFPI2) CLIA Kit
abx196292-96tests	Abbexa	96 tests	EUR 990

Human Tissue Factor Pathway Inhibitor 2 (TFPI2) CLIA Kit
20-abx493277	Abbexa	EUR 9567.60 EUR 5095.20 EUR 1177.20	10 × 96 tests 5 × 96 tests 96 tests

Mouse Tissue Factor Pathway Inhibitor 2 (TFPI2) ELISA Kit
abx254458-96tests	Abbexa	96 tests	EUR 848.4

Human Tissue Factor Pathway Inhibitor 2 (TFPI2) ELISA Kit
abx251335-96tests	Abbexa	96 tests	EUR 848.4

Sheep Tissue Factor Pathway Inhibitor 2 (TFPI2) ELISA Kit
abx364686-96tests	Abbexa	96 tests	EUR 1111.2

Mouse Tissue Factor Pathway Inhibitor 2 (TFPI2) ELISA Kit
20-abx585190	Abbexa	EUR 8853.60 EUR 4719.60 EUR 1093.20	10 × 96 tests 5 × 96 tests 96 tests

Human Tissue Factor Pathway Inhibitor 2 (TFPI2) ELISA Kit
abx575919-96tests	Abbexa	96 tests	EUR 848.4

Mouse Tissue Factor Pathway Inhibitor 2 (TFPI2) ELISA Kit
abx518899-96tests	Abbexa	96 tests	EUR 848.4

Human Tissue Factor Pathway Inhibitor 2 (TFPI2) ELISA Kit
DLR-TFPI2-Hu-48T	DL Develop	48T	EUR 597.6
Description: A sandwich quantitative ELISA assay kit for detection of Human Tissue Factor Pathway Inhibitor 2 (TFPI2) in samples from plasma.

Human Tissue Factor Pathway Inhibitor 2 (TFPI2) ELISA Kit
DLR-TFPI2-Hu-96T	DL Develop	96T	EUR 776.4
Description: A sandwich quantitative ELISA assay kit for detection of Human Tissue Factor Pathway Inhibitor 2 (TFPI2) in samples from plasma.

Human Tissue Factor Pathway Inhibitor 2 (TFPI2) ELISA Kit
20-abx153263	Abbexa	EUR 8534.40 EUR 4550.40 EUR 1054.80	10 × 96 tests 5 × 96 tests 96 tests

Mouse Tissue factor pathway inhibitor 2, Tfpi2 ELISA KIT
ELI-06238m	Lifescience Market	96 Tests	EUR 1038

Human Tissue factor pathway inhibitor 2, TFPI2 ELISA KIT
ELI-06239h	Lifescience Market	96 Tests	EUR 988.8

Human Tissue Factor Pathway Inhibitor 2 (TFPI2) ELISA Kit
SEB940Hu-10x96wellstestplate	Cloud-Clone	10x96-wells test plate	EUR 5402.92
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Tissue Factor Pathway Inhibitor 2 (TFPI2) in Plasma.

Human Tissue Factor Pathway Inhibitor 2 (TFPI2) ELISA Kit
SEB940Hu-1x48wellstestplate	Cloud-Clone	1x48-wells test plate	EUR 550.13
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Tissue Factor Pathway Inhibitor 2 (TFPI2) in Plasma.

Human Tissue Factor Pathway Inhibitor 2 (TFPI2) ELISA Kit
SEB940Hu-1x96wellstestplate	Cloud-Clone	1x96-wells test plate	EUR 734.46
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Tissue Factor Pathway Inhibitor 2 (TFPI2) in Plasma.

Human Tissue Factor Pathway Inhibitor 2 (TFPI2) ELISA Kit
SEB940Hu-5x96wellstestplate	Cloud-Clone	5x96-wells test plate	EUR 2945.08
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Tissue Factor Pathway Inhibitor 2 (TFPI2) in Plasma.

Human Tissue Factor Pathway Inhibitor 2 (TFPI2) ELISA Kit
4-SEB940Hu	Cloud-Clone	EUR 5463.60 EUR 2886.00 EUR 735.60	10 plates of 96 wells 5 plates of 96 wells 1 plate of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Tissue Factor Pathway Inhibitor 2 (TFPI2) in samples from Plasma. with no significant corss-reactivity with analogues from other species.

Mouse Tissue Factor Pathway Inhibitor 2 (TFPI2) ELISA Kit
SEB940Mu-10x96wellstestplate	Cloud-Clone	10x96-wells test plate	EUR 5834.88
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Tissue Factor Pathway Inhibitor 2 (TFPI2) in plasma.

Mouse Tissue Factor Pathway Inhibitor 2 (TFPI2) ELISA Kit
SEB940Mu-1x48wellstestplate	Cloud-Clone	1x48-wells test plate	EUR 585.7
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Tissue Factor Pathway Inhibitor 2 (TFPI2) in plasma.

Mouse Tissue Factor Pathway Inhibitor 2 (TFPI2) ELISA Kit
SEB940Mu-1x96wellstestplate	Cloud-Clone	1x96-wells test plate	EUR 785.28
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Tissue Factor Pathway Inhibitor 2 (TFPI2) in plasma.

Mouse Tissue Factor Pathway Inhibitor 2 (TFPI2) ELISA Kit
SEB940Mu-5x96wellstestplate	Cloud-Clone	5x96-wells test plate	EUR 3173.76
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Tissue Factor Pathway Inhibitor 2 (TFPI2) in plasma.

Mouse Tissue Factor Pathway Inhibitor 2 (TFPI2) ELISA Kit
4-SEB940Mu	Cloud-Clone	EUR 5895.60 EUR 3114.00 EUR 786.00	10 plates of 96 wells 5 plates of 96 wells 1 plate of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Mouse Tissue Factor Pathway Inhibitor 2 (TFPI2) in samples from plasma with no significant corss-reactivity with analogues from other species.

Human Tissue Factor Pathway Inhibitor 2 (TFPI2) ELISA Kit
RDR-TFPI2-Hu-48Tests	Reddot Biotech	48 Tests	EUR 626.4

Human Tissue Factor Pathway Inhibitor 2 (TFPI2) ELISA Kit
RDR-TFPI2-Hu-96Tests	Reddot Biotech	96 Tests	EUR 868.8

Human Tissue Factor Pathway Inhibitor 2 (TFPI2) ELISA Kit
RD-TFPI2-Hu-48Tests	Reddot Biotech	48 Tests	EUR 600

Human Tissue Factor Pathway Inhibitor 2 (TFPI2) ELISA Kit
RD-TFPI2-Hu-96Tests	Reddot Biotech	96 Tests	EUR 830.4

Human Tissue Factor Pathway Inhibitor 2 (TFPI2) ELISA Kit
RDR-TFPI2-Hu-48T	Reddot Biotech	48T	EUR 453.92
Description: plasma.

Human Tissue Factor Pathway Inhibitor 2 (TFPI2) ELISA Kit
RDR-TFPI2-Hu-96T	Reddot Biotech	96T	EUR 648.38
Description: plasma.

Human Tissue Factor Pathway Inhibitor 2 (TFPI2) ELISA Kit
RD-TFPI2-Hu-48T	Reddot Biotech	48T	EUR 432.3
Description: plasma.

Human Tissue Factor Pathway Inhibitor 2 (TFPI2) ELISA Kit
RD-TFPI2-Hu-96T	Reddot Biotech	96T	EUR 617.5
Description: plasma.

Rabbit Tissue Factor Pathway Inhibitor 2 (TFPI2) ELISA Kit
abx363282-96tests	Abbexa	96 tests	EUR 990

Monkey Tissue Factor Pathway Inhibitor 2 (TFPI2) ELISA Kit
abx360338-96tests	Abbexa	96 tests	EUR 990

Chicken Tissue Factor Pathway Inhibitor 2 (TFPI2) ELISA Kit
abx356836-96tests	Abbexa	96 tests	EUR 990

Mouse TFPI2(Tissue Factor Pathway Inhibitor 2) ELISA Kit
EM1399	FN Test	96T	EUR 628.92
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Mus ;Sensitivity: 0.094 ng/ml

Human TFPI2(Tissue factor pathway inhibitor 2) ELISA Kit
EH2010	FN Test	96T	EUR 628.92
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 46.875pg/ml

Mouse Tfpi2/ Tissue factor pathway inhibitor 2 ELISA Kit
E1454Mo	Sunlong	1 Kit	EUR 685.2

Human TFPI2/ Tissue factor pathway inhibitor 2 ELISA Kit
E2853Hu	Sunlong	1 Kit	EUR 685.2

Guinea pig Tissue Factor Pathway Inhibitor 2 (TFPI2) ELISA Kit
abx357941-96tests	Abbexa	96 tests	EUR 990

Tissue Factor Pathway Inhibitor 2 (TFPI2) Polyclonal Antibody (Rat)
4-PAB940Ra01	Cloud-Clone	EUR 301.20 EUR 3106.80 EUR 771.60 EUR 380.40 EUR 259.20	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Rat Tissue Factor Pathway Inhibitor 2 (TFPI2)

CLIA kit for Human TFPI2 (Tissue Factor Pathway Inhibitor 2)
E-CL-H1375	Elabscience Biotech	1 plate of 96 wells	EUR 700.8
Description: A sandwich CLIA kit for quantitative measurement of Human TFPI2 (Tissue Factor Pathway Inhibitor 2) in samples from Serum, Plasma, Cell supernatant

Tissue Factor Pathway Inhibitor 2 (TFPI2) Polyclonal Antibody (Human)
4-PAB940Hu01	Cloud-Clone	EUR 286.80 EUR 2869.20 EUR 717.60 EUR 358.80 EUR 253.20	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Human Tissue Factor Pathway Inhibitor 2 (TFPI2)

Tissue Factor Pathway Inhibitor 2 (TFPI2) Polyclonal Antibody (Mouse)
4-PAB940Mu01	Cloud-Clone	EUR 291.60 EUR 2948.40 EUR 735.60 EUR 366.00 EUR 254.40	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Mouse Tissue Factor Pathway Inhibitor 2 (TFPI2)

ELISA kit for Mouse TFPI2 (Tissue Factor Pathway Inhibitor 2)
E-EL-M2641	Elabscience Biotech	1 plate of 96 wells	EUR 640.8
Description: A sandwich ELISA kit for quantitative measurement of Mouse TFPI2 (Tissue Factor Pathway Inhibitor 2) in samples from Serum, Plasma, Cell supernatant

ELISA kit for Human TFPI2 (Tissue Factor Pathway Inhibitor 2)
E-EL-H2366	Elabscience Biotech	1 plate of 96 wells	EUR 640.8
Description: A sandwich ELISA kit for quantitative measurement of Human TFPI2 (Tissue Factor Pathway Inhibitor 2) in samples from Serum, Plasma, Cell supernatant

ELISA kit for Human TFPI2 (Tissue Factor Pathway Inhibitor 2)
ELK2691	ELK Biotech	1 plate of 96 wells	EUR 518.4
Description: A sandwich ELISA kit for detection of Tissue Factor Pathway Inhibitor 2 from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids.

Tissue Factor Pathway Inhibitor 2 (TFPI2) Polyclonal Antibody (Rat), PE
4-PAB940Ra01-PE	Cloud-Clone	EUR 362.40 EUR 3271.20 EUR 925.20 EUR 456.00 EUR 237.60	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Rat Tissue Factor Pathway Inhibitor 2 (TFPI2). This antibody is labeled with PE.

TFPI2 Tissue Factor Pathway Inhibitor 2 Human Recombinant Protein
PROTP48307	BosterBio	Regular: 5ug	EUR 380.4
Description: TFPI2 Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 199 amino acids (23-213 a.a.) and having a molecular mass of 22.9kDa (Migrates at 18-40kDa on SDS-PAGE under reducing conditions).;TFPI2 is expressed with an 8 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.

Tissue Factor Pathway Inhibitor 2 (TFPI2) Polyclonal Antibody (Rat), APC
4-PAB940Ra01-APC	Cloud-Clone	EUR 422.40 EUR 4059.60 EUR 1126.80 EUR 540.00 EUR 267.60	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Rat Tissue Factor Pathway Inhibitor 2 (TFPI2). This antibody is labeled with APC.

Tissue Factor Pathway Inhibitor 2 (TFPI2) Polyclonal Antibody (Rat), Cy3
4-PAB940Ra01-Cy3	Cloud-Clone	EUR 513.60 EUR 5362.80 EUR 1453.20 EUR 670.80 EUR 306.00	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Rat Tissue Factor Pathway Inhibitor 2 (TFPI2). This antibody is labeled with Cy3.

Tissue Factor Pathway Inhibitor 2 (TFPI2) Polyclonal Antibody (Rat), HRP
4-PAB940Ra01-HRP	Cloud-Clone	EUR 386.40 EUR 3537.60 EUR 996.00 EUR 488.40 EUR 252.00	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Rat Tissue Factor Pathway Inhibitor 2 (TFPI2). This antibody is labeled with HRP.

Tissue Factor Pathway Inhibitor 2 (TFPI2) Polyclonal Antibody (Human), APC
4-PAB940Hu01-APC	Cloud-Clone	EUR 399.60 EUR 3735.60 EUR 1046.40 EUR 507.60 EUR 258.00	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Human Tissue Factor Pathway Inhibitor 2 (TFPI2). This antibody is labeled with APC.

Tissue Factor Pathway Inhibitor 2 (TFPI2) Polyclonal Antibody (Human), Cy3
4-PAB940Hu01-Cy3	Cloud-Clone	EUR 483.60 EUR 4930.80 EUR 1345.20 EUR 627.60 EUR 294.00	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Human Tissue Factor Pathway Inhibitor 2 (TFPI2). This antibody is labeled with Cy3.

Tissue Factor Pathway Inhibitor 2 (TFPI2) Polyclonal Antibody (Human), HRP
4-PAB940Hu01-HRP	Cloud-Clone	EUR 366.00 EUR 3256.80 EUR 926.40 EUR 459.60 EUR 243.60	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Human Tissue Factor Pathway Inhibitor 2 (TFPI2). This antibody is labeled with HRP.

Tissue Factor Pathway Inhibitor 2 (TFPI2) Polyclonal Antibody (Human), PE
4-PAB940Hu01-PE	Cloud-Clone	EUR 344.40 EUR 3012.00 EUR 860.40 EUR 430.80 EUR 230.40	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Human Tissue Factor Pathway Inhibitor 2 (TFPI2). This antibody is labeled with PE.

Tissue Factor Pathway Inhibitor 2 (TFPI2) Polyclonal Antibody (Mouse), APC
4-PAB940Mu01-APC	Cloud-Clone	EUR 408.00 EUR 3843.60 EUR 1072.80 EUR 518.40 EUR 260.40	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Mouse Tissue Factor Pathway Inhibitor 2 (TFPI2). This antibody is labeled with APC.

Tissue Factor Pathway Inhibitor 2 (TFPI2) Polyclonal Antibody (Mouse), Cy3
4-PAB940Mu01-Cy3	Cloud-Clone	EUR 493.20 EUR 5074.80 EUR 1381.20 EUR 642.00 EUR 297.60	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Mouse Tissue Factor Pathway Inhibitor 2 (TFPI2). This antibody is labeled with Cy3.

Tissue Factor Pathway Inhibitor 2 (TFPI2) Polyclonal Antibody (Mouse), HRP
4-PAB940Mu01-HRP	Cloud-Clone	EUR 373.20 EUR 3350.40 EUR 949.20 EUR 469.20 EUR 246.00	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Mouse Tissue Factor Pathway Inhibitor 2 (TFPI2). This antibody is labeled with HRP.

Tissue Factor Pathway Inhibitor 2 (TFPI2) Polyclonal Antibody (Mouse), PE
4-PAB940Mu01-PE	Cloud-Clone	EUR 350.40 EUR 3098.40 EUR 882.00 EUR 439.20 EUR 232.80	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Mouse Tissue Factor Pathway Inhibitor 2 (TFPI2). This antibody is labeled with PE.

Tissue Factor Pathway Inhibitor 2 (TFPI2) Polyclonal Antibody (Rat), FITC
4-PAB940Ra01-FITC	Cloud-Clone	EUR 362.40 EUR 3271.20 EUR 925.20 EUR 456.00 EUR 237.60	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Rat Tissue Factor Pathway Inhibitor 2 (TFPI2). This antibody is labeled with FITC.

TFPI2 ELISA Kit| Mouse Tissue Factor Pathway Inhibitor 2 ELISA K
EF013927	Lifescience Market	96 Tests	EUR 826.8

Tissue Factor Pathway Inhibitor 2 (TFPI2) Polyclonal Antibody (Human), FITC
4-PAB940Hu01-FITC	Cloud-Clone	EUR 344.40 EUR 3012.00 EUR 860.40 EUR 430.80 EUR 230.40	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Human Tissue Factor Pathway Inhibitor 2 (TFPI2). This antibody is labeled with FITC.

Tissue Factor Pathway Inhibitor 2 (TFPI2) Polyclonal Antibody (Mouse), FITC
4-PAB940Mu01-FITC	Cloud-Clone	EUR 350.40 EUR 3098.40 EUR 882.00 EUR 439.20 EUR 232.80	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Mouse Tissue Factor Pathway Inhibitor 2 (TFPI2). This antibody is labeled with FITC.

Tissue Factor Pathway Inhibitor 2 (TFPI2) Polyclonal Antibody (Rat), APC-Cy7
4-PAB940Ra01-APC-Cy7	Cloud-Clone	EUR 702.00 EUR 7975.20 EUR 2110.80 EUR 937.20 EUR 391.20	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Rat Tissue Factor Pathway Inhibitor 2 (TFPI2). This antibody is labeled with APC-Cy7.

Tissue Factor Pathway Inhibitor 2 (TFPI2) Polyclonal Antibody (Rat), Biotinylated
4-PAB940Ra01-Biotin	Cloud-Clone	EUR 379.20 EUR 3046.80 EUR 896.40 EUR 465.60 EUR 265.20	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Rat Tissue Factor Pathway Inhibitor 2 (TFPI2). This antibody is labeled with Biotin.

Tissue Factor Pathway Inhibitor 2 (TFPI2) Polyclonal Antibody (Human), APC-Cy7
4-PAB940Hu01-APC-Cy7	Cloud-Clone	EUR 656.40 EUR 7327.20 EUR 1948.80 EUR 872.40 EUR 372.00	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Human Tissue Factor Pathway Inhibitor 2 (TFPI2). This antibody is labeled with APC-Cy7.

Tissue Factor Pathway Inhibitor 2 (TFPI2) Polyclonal Antibody (Mouse), APC-Cy7
4-PAB940Mu01-APC-Cy7	Cloud-Clone	EUR 672.00 EUR 7543.20 EUR 2002.80 EUR 894.00 EUR 378.00	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Mouse Tissue Factor Pathway Inhibitor 2 (TFPI2). This antibody is labeled with APC-Cy7.

Tissue Factor Pathway Inhibitor 2 (TFPI2) Polyclonal Antibody (Human), Biotinylated
4-PAB940Hu01-Biotin	Cloud-Clone	EUR 363.60 EUR 2809.20 EUR 836.40 EUR 442.80 EUR 259.20	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Human Tissue Factor Pathway Inhibitor 2 (TFPI2). This antibody is labeled with Biotin.

Tissue Factor Pathway Inhibitor 2 (TFPI2) Polyclonal Antibody (Mouse), Biotinylated
4-PAB940Mu01-Biotin	Cloud-Clone	EUR 368.40 EUR 2888.40 EUR 856.80 EUR 450.00 EUR 260.40	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Mouse Tissue Factor Pathway Inhibitor 2 (TFPI2). This antibody is labeled with Biotin.

Human Tissue factor pathway inhibitor (TFPI)
1-CSB-EP023437HU	Cusabio	EUR 456.00 EUR 256.80 EUR 1570.80 EUR 672.00 EUR 1047.60 EUR 314.40	100ug 10ug 1MG 200ug 500ug 50ug
Description: Recombinant Human Tissue factor pathway inhibitor(TFPI),partial expressed in E.coli

Rabbit Tissue factor pathway inhibitor (TFPI)
1-CSB-BP023437RB	Cusabio	EUR 1008.00 EUR 398.40 EUR 2604.00 EUR 1362.00 EUR 1894.80 EUR 564.00	100ug 10ug 1MG 200ug 500ug 50ug
Description: Recombinant Rabbit Tissue factor pathway inhibitor(TFPI) expressed in Baculovirus

Rabbit Tissue factor pathway inhibitor (TFPI)
1-CSB-YP023437RB	Cusabio	EUR 814.80 EUR 402.00 EUR 2606.40 EUR 1261.20 EUR 1730.40 EUR 522.00	100ug 10ug 1MG 200ug 500ug 50ug
Description: Recombinant Rabbit Tissue factor pathway inhibitor(TFPI) expressed in Yeast

Rabbit Tissue factor pathway inhibitor (TFPI)
1-CSB-EP023437RB	Cusabio	EUR 733.20 EUR 370.80 EUR 2192.40 EUR 1126.80 EUR 1461.60 EUR 476.40	100ug 10ug 1MG 200ug 500ug 50ug
Description: Recombinant Rabbit Tissue factor pathway inhibitor(TFPI) expressed in E.coli

Rabbit Tissue factor pathway inhibitor (TFPI)
1-CSB-MP023437RB	Cusabio	EUR 466.80 EUR 1672.80 EUR 675.60 EUR 1230.00	100ug 1MG 200ug 500ug
Description: Recombinant Rabbit Tissue factor pathway inhibitor(TFPI) expressed in Mammalian cell

Human Tissue factor pathway inhibitor 2,TFPI-2 ELISA Kit
201-12-1142	SunredBio	96 tests	EUR 528
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids.

Human Tissue factor pathway inhibitor 2, TFPI-2 ELISA Kit
CSB-E12803h-24T	Cusabio	1 plate of 24 wells	EUR 198
Description: Quantitativesandwich ELISA kit for measuring Human Tissue factor pathway inhibitor 2, TFPI-2 in samples from serum, plasma, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Human Tissue factor pathway inhibitor 2, TFPI-2 ELISA Kit
1-CSB-E12803h	Cusabio	EUR 964.80 EUR 6118.80 EUR 3244.80	1 plate of 96 wells 10 plates of 96 wells each 5 plates of 96 wells each
Description: Quantitativesandwich ELISA kit for measuring Human Tissue factor pathway inhibitor 2, TFPI-2 in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Human Tissue factor pathway inhibitor 2(TFPI-2)ELISA Kit
GA-E1158HM-48T	GenAsia Biotech	48T	EUR 346.8

Human Tissue factor pathway inhibitor 2(TFPI-2)ELISA Kit
GA-E1158HM-96T	GenAsia Biotech	96T	EUR 559.2

Human Tissue factor pathway inhibitor 2(TFPI-2)ELISA Kit
QY-E00005	Qayee Biotechnology	96T	EUR 433.2

Human Tissue factor pathway inhibitor 2,TFPI-2 ELISA Kit
YLA1734HU-48T	Shanghai YL Biotech	48T	EUR 435

Human Tissue factor pathway inhibitor 2,TFPI-2 ELISA Kit
YLA1734HU-96T	Shanghai YL Biotech	96T	EUR 562.5

Tissue Factor Pathway Inhibitor (TFPI) Protein
20-abx261170	Abbexa	EUR 4101.60 EUR 393.60 EUR 276.00	1 mg 20 ug 5 ug

Tissue Factor Pathway Inhibitor (TFPI) Antibody
abx238634-100ug	Abbexa	100 ug	EUR 661.2

Tissue Factor Pathway Inhibitor (TFPI) Antibody
20-abx103325	Abbexa	EUR 376.80 EUR 159.60 EUR 994.80 EUR 526.80 EUR 326.40	100 ug 10 ug 1 mg 200 ug 50 ug

Tissue Factor Pathway Inhibitor (TFPI) Antibody
20-abx103326	Abbexa	EUR 376.80 EUR 159.60 EUR 994.80 EUR 526.80 EUR 326.40	100 ug 10 ug 1 mg 200 ug 50 ug

Tissue Factor Pathway Inhibitor (TFPI) Antibody
20-abx103328	Abbexa	EUR 510.00 EUR 159.60 EUR 1412.40 EUR 693.60 EUR 393.60	100 ug 10 ug 1 mg 200 ug 50 ug

Tissue Factor Pathway Inhibitor (TFPI) Antibody
20-abx103329	Abbexa	EUR 510.00 EUR 159.60 EUR 1412.40 EUR 693.60 EUR 393.60	100 ug 10 ug 1 mg 200 ug 50 ug

Tissue Factor Pathway Inhibitor (TFPI) Antibody
20-abx104947	Abbexa	EUR 376.80 EUR 159.60 EUR 994.80 EUR 526.80 EUR 326.40	100 ug 10 ug 1 mg 200 ug 50 ug

Tissue Factor Pathway Inhibitor (TFPI) Antibody
20-abx174804	Abbexa	EUR 1011.60 EUR 526.80	1 mg 200 ug

Tissue Factor Pathway Inhibitor (TFPI) Antibody
20-abx116110	Abbexa	EUR 878.40 EUR 477.60	150 ul 50 ul

Tissue Factor Pathway Inhibitor (TFPI) Antibody
20-abx129517	Abbexa	EUR 493.20 EUR 159.60 EUR 1362.00 EUR 661.20 EUR 376.80	100 ug 10 ug 1 mg 200 ug 50 ug

Tissue Factor Pathway Inhibitor (TFPI) Antibody
20-abx132084	Abbexa	EUR 360.00 EUR 844.80 EUR 427.20 EUR 184.80 EUR 292.80	100 ug 1 mg 200 ug 20 ug 50 ug

Tissue Factor Pathway Inhibitor (TFPI) Antibody
20-abx132085	Abbexa	EUR 393.60 EUR 1011.60 EUR 526.80 EUR 184.80 EUR 309.60	100 ug 1 mg 200 ug 20 ug 50 ug

Tissue Factor Pathway Inhibitor (TFPI) Antibody
20-abx132086	Abbexa	EUR 360.00 EUR 844.80 EUR 427.20 EUR 184.80 EUR 292.80	100 ug 1 mg 200 ug 20 ug 50 ug

Tissue Factor Pathway Inhibitor (TFPI) Antibody
20-abx323593	Abbexa	EUR 376.80 EUR 292.80	100 ug 50 ug

Tissue Factor Pathway Inhibitor (TFPI) Antibody
abx433382-200ul	Abbexa	200 ul	EUR 460.8

Tissue Factor Pathway Inhibitor (TFPI) Antibody
20-abx001368	Abbexa	EUR 493.20 EUR 710.40 EUR 218.40 EUR 376.80	100 ul 200 ul 20 ul 50 ul

Right here, we current a fast protocol to provide extremely pure and monomeric recombinant Aβ utilizing a one-step ion change purification methodology and to label the peptide utilizing a maleimide dye. The washing, solublisation and purification steps take solely three hours. We additionally current a protocol for the isolation of Aβ-mCherry from mammalian cells.