In Chapter 3 , we described the Structural Genomics Consortium (SGC) course of for producing a number of constructs of truncated variations of every protein utilizing LIC. On this chapter we offer a step-by-step process of our E. coli system for take a look at expressing intracellular (soluble) proteins in a 96-well format that allows us to determine which proteins or truncated variations are expressed in a soluble and secure kind appropriate for structural research. As well as, we element the method for scaling up cultures for large-scale protein purification.
This stage of manufacturing is required to acquire enough portions (i.e., milligram quantities) of protein for additional characterization and/or structural research (e.g., crystallization or cryo-EM experiments). Our normal course of is purification by immobilized steel affinity chromatography (IMAC) utilizing nickel resin adopted by dimension exclusion chromatography (SEC), with further procedures arising from the complexity of the protein itself.
Examine of the results of three.1 THz radiation on the expression of recombinant purple fluorescent protein (RFP) in E. coli
Lately, many research have been performed to research the non-thermal results of THz radiation on completely different organisms, however additional research are wanted to totally elucidate the results, particularly on the molecular stage. On this research, we explored the results of at 3.1 THz radiation on protein expression in Escherichia coli (E. coli) utilizing purple fluorescent protein as a reporter molecule.
After Eight hours of steady THz irradiation of micro organism on LB (Luria-Bertani) stable plates at a mean energy of 33 mW/cm2 and 10 Hz pulse repetition frequency, we discovered that the plasmid copy quantity, protein expression and fluorescence depth of micro organism from the irradiated space have been 3.8-, 2.7-, and three.
Three occasions greater than in micro organism from the un-irradiated space, respectively. These findings recommend that plasmid replication modified considerably in micro organism uncovered to three.1 THz radiation, leading to elevated protein expression as evidenced by elevated fluorescence depth of the RFP reporter.
Expression of Recombinant Leptospiral Floor Lipoprotein-Lsa27 in E. coli and Its Evaluation for Serodiagnosis of Bovine Leptospirosis by Latex Agglutination Check
The expressed recombinant leptospiral floor adhesion lipoprotein (Lsa27) of pathogenic Leptospira in E. coli was evaluated for the detection of Leptospira antibodies in cattle sera by latex agglutination take a look at (LAT). The Lsa27 missing sign peptide coding gene sequences from L. interrogans serovar Pomona was amplified (~ 660 bp) by PCR and the amplicon was cloned into pETiteN-HisKan vector.
The expressed recombinant Lsa27 histidine-tagged fusion protein (rLsa27) was Ni-NTA affinity purified below denaturation adopted by renaturation strategies. The purified rLsa27 was characterised by SDS-PAGE and immunoblot, which confirmed the leptospiral protein with a MW of ~ 25 kDa. Additional, the ready sensitized latex beads coated with rLsa27 have been evaluated as a diagnostic antigen for detection of pathogenic Leptospira antibodies by utilizing identified microscopic agglutination take a look at (MAT) constructive (n = 74) and detrimental (n = 62) sera for Leptospira antibodies in LAT, which revealed the relative diagnostic sensitivity of 91.89% and specificity of 87.10% in opposition to the gold normal serological take a look at, MAT.
Moreover, on analysis of developed rLsa27 LAT utilizing serum samples from cattle related to the historical past of abortions and reproductive dysfunction (n = 309), the relative sensitivity of 96.15%, and specificity of 89.11% have been noticed. Due to this fact, this fast subject take a look at utilizing the rLsa27 is first of its form and it could possibly be used as a screening take a look at for the detection of Leptospira antibodies or it may be complemented by different diagnostics for the analysis /surveillance of bovine leptospirosis.

E. coli expression and immunological evaluation of expressed recombinant Newcastle illness virus hemagglutinin-neuraminidase protein in chickens
Yearly, the poultry trade experiences important financial losses as a consequence of epidemics of Newcastle illness virus (NDV). Growing new vaccines by figuring out and utilizing the immunogenic hemagglutinin-neuraminidase (HN) protein can defend the poultry trade. Within the current research, the full-length HN protein was expressed in Escherichia coli (E. coli) BL21 (DE3) cells, purified by way of affinity chromatography and detected by way of western blot evaluation utilizing His-specific antibodies.
The purified HN protein was additional evaluated in chickens to review the immune response in opposition to NDV. The profitable manufacturing of HN-specific IgY proved the exercise of the purified HN protein. IgY was current within the serum of immunized chickens. Nonetheless, the immune response was greater in chickens immunized with purified HN protein together with full and incomplete adjuvants than in chickens immunized with solely the HN protein. Key phrases: protein; Newcastle illness virus; poultry; infectious illnesses; vaccines.
Quick purification of recombinant monomeric amyloid-β from E. coli and amyloid-β-mCherry aggregates from mammalian cells
The Alzheimer’s illness associated peptide, Amyloid-beta (Aβ)1-40 and 1-42, has confirmed tough to be purified as a recombinant monomeric protein due its expression in E. coli resulting in the formation of insoluble inclusion our bodies and its tendency to rapidly kind insoluble aggregates.
An enormous array of strategies have been used to date, but many have pitfalls, resembling using tags for ease of Aβ isolation, the formation of Aβ multimers inside the timeframe of extraction or the necessity to reconstitute Aβ from a freeze dried state.
Recombinant Tissue Factor Pathway Inhibitor 2 (TFPI2) |
4-RPB940Bo01 |
Cloud-Clone |
-
EUR 558.75
-
EUR 253.00
-
EUR 1820.32
-
EUR 673.44
-
EUR 1246.88
-
EUR 437.00
-
EUR 4400.80
|
-
100 ug
-
10ug
-
1 mg
-
200 ug
-
500 ug
-
50ug
-
5 mg
|
|
Description: Recombinant Bovine Tissue Factor Pathway Inhibitor 2 expressed in: E.coli |
Recombinant Tissue Factor Pathway Inhibitor 2 (TFPI2) |
4-RPB940Hu01 |
Cloud-Clone |
-
EUR 458.40
-
EUR 226.00
-
EUR 1444.00
-
EUR 548.00
-
EUR 996.00
-
EUR 370.00
-
EUR 3460.00
|
-
100 ug
-
10ug
-
1 mg
-
200 ug
-
500 ug
-
50ug
-
5 mg
|
|
Description: Recombinant Human Tissue Factor Pathway Inhibitor 2 expressed in: E.coli |
Recombinant Tissue Factor Pathway Inhibitor 2 (TFPI2) |
4-RPB940Mu01 |
Cloud-Clone |
-
EUR 503.20
-
EUR 238.00
-
EUR 1612.00
-
EUR 604.00
-
EUR 1108.00
-
EUR 400.00
-
EUR 3880.00
|
-
100 ug
-
10ug
-
1 mg
-
200 ug
-
500 ug
-
50ug
-
5 mg
|
|
Description: Recombinant Mouse Tissue Factor Pathway Inhibitor 2 expressed in: E.coli |
Recombinant Tissue Factor Pathway Inhibitor 2 (TFPI2) |
4-RPB940Ra01 |
Cloud-Clone |
-
EUR 548.00
-
EUR 250.00
-
EUR 1780.00
-
EUR 660.00
-
EUR 1220.00
-
EUR 430.00
-
EUR 4300.00
|
-
100 ug
-
10ug
-
1 mg
-
200 ug
-
500 ug
-
50ug
-
5 mg
|
|
Description: Recombinant Rat Tissue Factor Pathway Inhibitor 2 expressed in: E.coli |
Tissue Factor Pathway Inhibitor 2 (TFPI2) Antibody |
abx122715-100ug |
Abbexa |
100 ug |
EUR 391 |
|
Tissue Factor Pathway Inhibitor 2 (TFPI2) Antibody |
20-abx320289 |
Abbexa |
|
|
|
Tissue Factor Pathway Inhibitor 2 (TFPI2) Antibody |
20-abx322259 |
Abbexa |
|
|
|
Tissue Factor Pathway Inhibitor 2 (TFPI2) Antibody |
20-abx323594 |
Abbexa |
|
|
|
Tissue Factor Pathway Inhibitor 2 (TFPI2) Antibody |
20-abx339482 |
Abbexa |
|
|
|
Tissue Factor Pathway Inhibitor 2 (TFPI2) Antibody |
20-abx210903 |
Abbexa |
|
|
|
Tissue Factor Pathway Inhibitor 2 (TFPI2) Antibody |
20-abx174805 |
Abbexa |
|
|
|
Tissue Factor Pathway Inhibitor 2 (TFPI2) Antibody |
20-abx103659 |
Abbexa |
-
EUR 411.00
-
EUR 133.00
-
EUR 1149.00
-
EUR 565.00
-
EUR 314.00
|
-
100 ug
-
10 ug
-
1 mg
-
200 ug
-
50 ug
|
|
Tissue Factor Pathway Inhibitor 2 (TFPI2) Antibody |
20-abx103660 |
Abbexa |
-
EUR 425.00
-
EUR 133.00
-
EUR 1177.00
-
EUR 578.00
-
EUR 328.00
|
-
100 ug
-
10 ug
-
1 mg
-
200 ug
-
50 ug
|
|
Tissue Factor Pathway Inhibitor 2 (TFPI2) Antibody |
20-abx103661 |
Abbexa |
-
EUR 439.00
-
EUR 133.00
-
EUR 1247.00
-
EUR 592.00
-
EUR 328.00
|
-
100 ug
-
10 ug
-
1 mg
-
200 ug
-
50 ug
|
|
Tissue Factor Pathway Inhibitor 2 (TFPI2) Antibody |
20-abx103662 |
Abbexa |
-
EUR 467.00
-
EUR 133.00
-
EUR 1344.00
-
EUR 634.00
-
EUR 356.00
|
-
100 ug
-
10 ug
-
1 mg
-
200 ug
-
50 ug
|
|
Tissue Factor Pathway Inhibitor 2 (TFPI2) Antibody |
20-abx006327 |
Abbexa |
-
EUR 411.00
-
EUR 592.00
-
EUR 182.00
-
EUR 314.00
|
-
100 ul
-
200 ul
-
20 ul
-
50 ul
|
|
Tissue Factor Pathway Inhibitor 2 (TFPI2) Polyclonal Antibody (Bovine), APC |
4-PAB940Bo01-APC |
Cloud-Clone |
-
EUR 376.00
-
EUR 3707.00
-
EUR 1020.00
-
EUR 483.00
-
EUR 232.00
|
-
100ul
-
10ml
-
1ml
-
200ul
-
20ul
|
|
Description: A Rabbit polyclonal antibody against Bovine Tissue Factor Pathway Inhibitor 2 (TFPI2). This antibody is labeled with APC. |
Tissue Factor Pathway Inhibitor 2 (TFPI2) Polyclonal Antibody (Bovine), Biotinylated |
4-PAB940Bo01-Biotin |
Cloud-Clone |
-
EUR 334.00
-
EUR 2776.00
-
EUR 806.00
-
EUR 412.00
-
EUR 228.00
|
-
100ul
-
10ml
-
1ml
-
200ul
-
20ul
|
|
Description: A Rabbit polyclonal antibody against Bovine Tissue Factor Pathway Inhibitor 2 (TFPI2). This antibody is labeled with Biotin. |
Tissue Factor Pathway Inhibitor 2 (TFPI2) Polyclonal Antibody (Bovine), Cy3 |
4-PAB940Bo01-Cy3 |
Cloud-Clone |
-
EUR 459.00
-
EUR 4901.00
-
EUR 1319.00
-
EUR 602.00
-
EUR 267.00
|
-
100ul
-
10ml
-
1ml
-
200ul
-
20ul
|
|
Description: A Rabbit polyclonal antibody against Bovine Tissue Factor Pathway Inhibitor 2 (TFPI2). This antibody is labeled with Cy3. |
Tissue Factor Pathway Inhibitor 2 (TFPI2) Polyclonal Antibody (Bovine), FITC |
4-PAB940Bo01-FITC |
Cloud-Clone |
-
EUR 320.00
-
EUR 2985.00
-
EUR 836.00
-
EUR 406.00
-
EUR 205.00
|
-
100ul
-
10ml
-
1ml
-
200ul
-
20ul
|
|
Description: A Rabbit polyclonal antibody against Bovine Tissue Factor Pathway Inhibitor 2 (TFPI2). This antibody is labeled with FITC. |
Tissue Factor Pathway Inhibitor 2 (TFPI2) Polyclonal Antibody (Bovine), HRP |
4-PAB940Bo01-HRP |
Cloud-Clone |
-
EUR 342.00
-
EUR 3229.00
-
EUR 901.00
-
EUR 435.00
-
EUR 217.00
|
-
100ul
-
10ml
-
1ml
-
200ul
-
20ul
|
|
Description: A Rabbit polyclonal antibody against Bovine Tissue Factor Pathway Inhibitor 2 (TFPI2). This antibody is labeled with HRP. |
Tissue Factor Pathway Inhibitor 2 (TFPI2) Polyclonal Antibody (Bovine), PE |
4-PAB940Bo01-PE |
Cloud-Clone |
-
EUR 320.00
-
EUR 2985.00
-
EUR 836.00
-
EUR 406.00
-
EUR 205.00
|
-
100ul
-
10ml
-
1ml
-
200ul
-
20ul
|
|
Description: A Rabbit polyclonal antibody against Bovine Tissue Factor Pathway Inhibitor 2 (TFPI2). This antibody is labeled with PE. |
ELISA kit for Human TFPI2 (Tissue Factor Pathway Inhibitor 2) |
E-EL-H2366 |
Elabscience Biotech |
1 plate of 96 wells |
EUR 534 |
|
Description: A sandwich ELISA kit for quantitative measurement of Human TFPI2 (Tissue Factor Pathway Inhibitor 2) in samples from Serum, Plasma, Cell supernatant |
ELISA kit for Mouse TFPI2 (Tissue Factor Pathway Inhibitor 2) |
E-EL-M2641 |
Elabscience Biotech |
1 plate of 96 wells |
EUR 534 |
|
Description: A sandwich ELISA kit for quantitative measurement of Mouse TFPI2 (Tissue Factor Pathway Inhibitor 2) in samples from Serum, Plasma, Cell supernatant |
CLIA kit for Human TFPI2 (Tissue Factor Pathway Inhibitor 2) |
E-CL-H1375 |
Elabscience Biotech |
1 plate of 96 wells |
EUR 584 |
|
Description: A sandwich CLIA kit for quantitative measurement of Human TFPI2 (Tissue Factor Pathway Inhibitor 2) in samples from Serum, Plasma, Cell supernatant |
Human Tissue Factor Pathway Inhibitor 2 (TFPI2) Protein |
20-abx069324 |
Abbexa |
-
EUR 648.00
-
EUR 272.00
-
EUR 1943.00
-
EUR 759.00
-
EUR 467.00
|
-
100 ug
-
10 ug
-
1 mg
-
200 ug
-
50 ug
|
|
Rat Tissue Factor Pathway Inhibitor 2 (TFPI2) Protein |
20-abx069325 |
Abbexa |
-
EUR 759.00
-
EUR 300.00
-
EUR 2388.00
-
EUR 913.00
-
EUR 537.00
|
-
100 ug
-
10 ug
-
1 mg
-
200 ug
-
50 ug
|
|
Mouse Tissue Factor Pathway Inhibitor 2 (TFPI2) Protein |
20-abx069326 |
Abbexa |
-
EUR 704.00
-
EUR 286.00
-
EUR 2165.00
-
EUR 829.00
-
EUR 495.00
|
-
100 ug
-
10 ug
-
1 mg
-
200 ug
-
50 ug
|
|
Cow Tissue Factor Pathway Inhibitor 2 (TFPI2) Protein |
20-abx069327 |
Abbexa |
-
EUR 773.00
-
EUR 300.00
-
EUR 2444.00
-
EUR 926.00
-
EUR 551.00
|
-
100 ug
-
10 ug
-
1 mg
-
200 ug
-
50 ug
|
|
Tissue Factor Pathway Inhibitor 2 (TFPI2) Polyclonal Antibody (Bovine), APC-Cy7 |
4-PAB940Bo01-APC-Cy7 |
Cloud-Clone |
-
EUR 632.00
-
EUR 7294.00
-
EUR 1921.00
-
EUR 846.00
-
EUR 344.00
|
-
100ul
-
10ml
-
1ml
-
200ul
-
20ul
|
|
Description: A Rabbit polyclonal antibody against Bovine Tissue Factor Pathway Inhibitor 2 (TFPI2). This antibody is labeled with APC-Cy7. |
Mouse Tissue factor pathway inhibitor 2, Tfpi2 ELISA KIT |
ELI-06238m |
Lifescience Market |
96 Tests |
EUR 865 |
Human Tissue factor pathway inhibitor 2, TFPI2 ELISA KIT |
ELI-06239h |
Lifescience Market |
96 Tests |
EUR 824 |
Tissue Factor Pathway Inhibitor 2 (TFPI2) Polyclonal Antibody (Human) |
4-PAB940Hu01 |
Cloud-Clone |
-
EUR 239.00
-
EUR 2391.00
-
EUR 598.00
-
EUR 299.00
-
EUR 211.00
|
-
100ul
-
10ml
-
1ml
-
200ul
-
20ul
|
|
Description: A Rabbit polyclonal antibody against Human Tissue Factor Pathway Inhibitor 2 (TFPI2) |
Tissue Factor Pathway Inhibitor 2 (TFPI2) Polyclonal Antibody (Mouse) |
4-PAB940Mu01 |
Cloud-Clone |
-
EUR 243.00
-
EUR 2457.00
-
EUR 613.00
-
EUR 305.00
-
EUR 212.00
|
-
100ul
-
10ml
-
1ml
-
200ul
-
20ul
|
|
Description: A Rabbit polyclonal antibody against Mouse Tissue Factor Pathway Inhibitor 2 (TFPI2) |
Tissue Factor Pathway Inhibitor 2 (TFPI2) Polyclonal Antibody (Rat) |
4-PAB940Ra01 |
Cloud-Clone |
-
EUR 251.00
-
EUR 2589.00
-
EUR 643.00
-
EUR 317.00
-
EUR 216.00
|
-
100ul
-
10ml
-
1ml
-
200ul
-
20ul
|
|
Description: A Rabbit polyclonal antibody against Rat Tissue Factor Pathway Inhibitor 2 (TFPI2) |
Human Tissue Factor Pathway Inhibitor 2 (TFPI2) ELISA Kit |
SEB940Hu-10x96wellstestplate |
Cloud-Clone |
10x96-wells test plate |
EUR 4502.43 |
|
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Tissue Factor Pathway Inhibitor 2 (TFPI2) in Plasma. |
Human Tissue Factor Pathway Inhibitor 2 (TFPI2) ELISA Kit |
SEB940Hu-1x48wellstestplate |
Cloud-Clone |
1x48-wells test plate |
EUR 458.44 |
|
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Tissue Factor Pathway Inhibitor 2 (TFPI2) in Plasma. |
Human Tissue Factor Pathway Inhibitor 2 (TFPI2) ELISA Kit |
SEB940Hu-1x96wellstestplate |
Cloud-Clone |
1x96-wells test plate |
EUR 612.05 |
|
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Tissue Factor Pathway Inhibitor 2 (TFPI2) in Plasma. |
Human Tissue Factor Pathway Inhibitor 2 (TFPI2) ELISA Kit |
SEB940Hu-5x96wellstestplate |
Cloud-Clone |
5x96-wells test plate |
EUR 2454.23 |
|
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Tissue Factor Pathway Inhibitor 2 (TFPI2) in Plasma. |
Human Tissue Factor Pathway Inhibitor 2 (TFPI2) ELISA Kit |
4-SEB940Hu |
Cloud-Clone |
-
EUR 4553.00
-
EUR 2405.00
-
EUR 613.00
|
-
10 plates of 96 wells
-
5 plates of 96 wells
-
1 plate of 96 wells
|
|
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Tissue Factor Pathway Inhibitor 2 (TFPI2) in samples from Plasma. with no significant corss-reactivity with analogues from other species. |
Mouse Tissue Factor Pathway Inhibitor 2 (TFPI2) ELISA Kit |
SEB940Mu-10x96wellstestplate |
Cloud-Clone |
10x96-wells test plate |
EUR 4862.4 |
|
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Tissue Factor Pathway Inhibitor 2 (TFPI2) in plasma. |
Mouse Tissue Factor Pathway Inhibitor 2 (TFPI2) ELISA Kit |
SEB940Mu-1x48wellstestplate |
Cloud-Clone |
1x48-wells test plate |
EUR 488.08 |
|
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Tissue Factor Pathway Inhibitor 2 (TFPI2) in plasma. |
Mouse Tissue Factor Pathway Inhibitor 2 (TFPI2) ELISA Kit |
SEB940Mu-1x96wellstestplate |
Cloud-Clone |
1x96-wells test plate |
EUR 654.4 |
|
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Tissue Factor Pathway Inhibitor 2 (TFPI2) in plasma. |
Mouse Tissue Factor Pathway Inhibitor 2 (TFPI2) ELISA Kit |
SEB940Mu-5x96wellstestplate |
Cloud-Clone |
5x96-wells test plate |
EUR 2644.8 |
|
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Tissue Factor Pathway Inhibitor 2 (TFPI2) in plasma. |
Mouse Tissue Factor Pathway Inhibitor 2 (TFPI2) ELISA Kit |
4-SEB940Mu |
Cloud-Clone |
-
EUR 4913.00
-
EUR 2595.00
-
EUR 655.00
|
-
10 plates of 96 wells
-
5 plates of 96 wells
-
1 plate of 96 wells
|
|
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Mouse Tissue Factor Pathway Inhibitor 2 (TFPI2) in samples from plasma with no significant corss-reactivity with analogues from other species. |
Human Tissue Factor Pathway Inhibitor 2 (TFPI2) ELISA Kit |
abx575919-96tests |
Abbexa |
96 tests |
EUR 707 |
|
Human Tissue Factor Pathway Inhibitor 2 (TFPI2) CLIA Kit |
20-abx493277 |
Abbexa |
-
EUR 7973.00
-
EUR 4246.00
-
EUR 981.00
|
-
10 × 96 tests
-
5 × 96 tests
-
96 tests
|
|
Cow Tissue Factor Pathway Inhibitor 2 (TFPI2) ELISA Kit |
abx518897-96tests |
Abbexa |
96 tests |
EUR 911 |
|
Mouse Tissue Factor Pathway Inhibitor 2 (TFPI2) ELISA Kit |
abx518899-96tests |
Abbexa |
96 tests |
EUR 707 |
|
Sheep Tissue Factor Pathway Inhibitor 2 (TFPI2) ELISA Kit |
abx364686-96tests |
Abbexa |
96 tests |
EUR 926 |
|
Monkey Tissue Factor Pathway Inhibitor 2 (TFPI2) ELISA Kit |
abx360338-96tests |
Abbexa |
96 tests |
EUR 825 |
|
Pig Tissue Factor Pathway Inhibitor 2 (TFPI2) ELISA Kit |
abx362050-96tests |
Abbexa |
96 tests |
EUR 825 |
|
Rabbit Tissue Factor Pathway Inhibitor 2 (TFPI2) ELISA Kit |
abx363282-96tests |
Abbexa |
96 tests |
EUR 825 |
|
Chicken Tissue Factor Pathway Inhibitor 2 (TFPI2) ELISA Kit |
abx356836-96tests |
Abbexa |
96 tests |
EUR 825 |
|
Mouse Tissue Factor Pathway Inhibitor 2 (TFPI2) ELISA Kit |
20-abx585190 |
Abbexa |
-
EUR 7378.00
-
EUR 3933.00
-
EUR 911.00
|
-
10 × 96 tests
-
5 × 96 tests
-
96 tests
|
|
Human Tissue Factor Pathway Inhibitor 2 (TFPI2) CLIA Kit |
abx196292-96tests |
Abbexa |
96 tests |
EUR 825 |
|
Human Tissue Factor Pathway Inhibitor 2 (TFPI2) ELISA Kit |
abx251335-96tests |
Abbexa |
96 tests |
EUR 707 |
|
Mouse Tissue Factor Pathway Inhibitor 2 (TFPI2) ELISA Kit |
abx254458-96tests |
Abbexa |
96 tests |
EUR 707 |
|
Human Tissue Factor Pathway Inhibitor 2 (TFPI2) ELISA Kit |
20-abx153263 |
Abbexa |
-
EUR 7112.00
-
EUR 3792.00
-
EUR 879.00
|
-
10 × 96 tests
-
5 × 96 tests
-
96 tests
|
|
TFPI2 Tissue Factor Pathway Inhibitor 2 Human Recombinant Protein |
PROTP48307 |
BosterBio |
Regular: 5ug |
EUR 317 |
Description: TFPI2 Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 199 amino acids (23-213 a.a.) and having a molecular mass of 22.9kDa (Migrates at 18-40kDa on SDS-PAGE under reducing conditions).;TFPI2 is expressed with an 8 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques. |
Human TFPI2/ Tissue factor pathway inhibitor 2 ELISA Kit |
E2853Hu |
Sunlong |
1 Kit |
EUR 571 |
Mouse Tfpi2/ Tissue factor pathway inhibitor 2 ELISA Kit |
E1454Mo |
Sunlong |
1 Kit |
EUR 571 |
Mouse TFPI2(Tissue Factor Pathway Inhibitor 2) ELISA Kit |
EM1399 |
FN Test |
96T |
EUR 524.1 |
|
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Mus ;Sensitivity: 0.094 ng/ml |
Human TFPI2(Tissue factor pathway inhibitor 2) ELISA Kit |
EH2010 |
FN Test |
96T |
EUR 524.1 |
|
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 46.875pg/ml |
Tissue Factor Pathway Inhibitor 2 (TFPI2) Polyclonal Antibody (Human), APC |
4-PAB940Hu01-APC |
Cloud-Clone |
-
EUR 333.00
-
EUR 3113.00
-
EUR 872.00
-
EUR 423.00
-
EUR 215.00
|
-
100ul
-
10ml
-
1ml
-
200ul
-
20ul
|
|
Description: A Rabbit polyclonal antibody against Human Tissue Factor Pathway Inhibitor 2 (TFPI2). This antibody is labeled with APC. |
Tissue Factor Pathway Inhibitor 2 (TFPI2) Polyclonal Antibody (Human), Biotinylated |
4-PAB940Hu01-Biotin |
Cloud-Clone |
-
EUR 303.00
-
EUR 2341.00
-
EUR 697.00
-
EUR 369.00
-
EUR 216.00
|
-
100ul
-
10ml
-
1ml
-
200ul
-
20ul
|
|
Description: A Rabbit polyclonal antibody against Human Tissue Factor Pathway Inhibitor 2 (TFPI2). This antibody is labeled with Biotin. |
Tissue Factor Pathway Inhibitor 2 (TFPI2) Polyclonal Antibody (Human), Cy3 |
4-PAB940Hu01-Cy3 |
Cloud-Clone |
-
EUR 403.00
-
EUR 4109.00
-
EUR 1121.00
-
EUR 523.00
-
EUR 245.00
|
-
100ul
-
10ml
-
1ml
-
200ul
-
20ul
|
|
Description: A Rabbit polyclonal antibody against Human Tissue Factor Pathway Inhibitor 2 (TFPI2). This antibody is labeled with Cy3. |
Tissue Factor Pathway Inhibitor 2 (TFPI2) Polyclonal Antibody (Human), FITC |
4-PAB940Hu01-FITC |
Cloud-Clone |
-
EUR 287.00
-
EUR 2510.00
-
EUR 717.00
-
EUR 359.00
-
EUR 192.00
|
-
100ul
-
10ml
-
1ml
-
200ul
-
20ul
|
|
Description: A Rabbit polyclonal antibody against Human Tissue Factor Pathway Inhibitor 2 (TFPI2). This antibody is labeled with FITC. |
Tissue Factor Pathway Inhibitor 2 (TFPI2) Polyclonal Antibody (Human), HRP |
4-PAB940Hu01-HRP |
Cloud-Clone |
-
EUR 305.00
-
EUR 2714.00
-
EUR 772.00
-
EUR 383.00
-
EUR 203.00
|
-
100ul
-
10ml
-
1ml
-
200ul
-
20ul
|
|
Description: A Rabbit polyclonal antibody against Human Tissue Factor Pathway Inhibitor 2 (TFPI2). This antibody is labeled with HRP. |
Tissue Factor Pathway Inhibitor 2 (TFPI2) Polyclonal Antibody (Human), PE |
4-PAB940Hu01-PE |
Cloud-Clone |
-
EUR 287.00
-
EUR 2510.00
-
EUR 717.00
-
EUR 359.00
-
EUR 192.00
|
-
100ul
-
10ml
-
1ml
-
200ul
-
20ul
|
|
Description: A Rabbit polyclonal antibody against Human Tissue Factor Pathway Inhibitor 2 (TFPI2). This antibody is labeled with PE. |
Tissue Factor Pathway Inhibitor 2 (TFPI2) Polyclonal Antibody (Mouse), APC |
4-PAB940Mu01-APC |
Cloud-Clone |
-
EUR 340.00
-
EUR 3203.00
-
EUR 894.00
-
EUR 432.00
-
EUR 217.00
|
-
100ul
-
10ml
-
1ml
-
200ul
-
20ul
|
|
Description: A Rabbit polyclonal antibody against Mouse Tissue Factor Pathway Inhibitor 2 (TFPI2). This antibody is labeled with APC. |
Tissue Factor Pathway Inhibitor 2 (TFPI2) Polyclonal Antibody (Mouse), Biotinylated |
4-PAB940Mu01-Biotin |
Cloud-Clone |
-
EUR 307.00
-
EUR 2407.00
-
EUR 714.00
-
EUR 375.00
-
EUR 217.00
|
-
100ul
-
10ml
-
1ml
-
200ul
-
20ul
|
|
Description: A Rabbit polyclonal antibody against Mouse Tissue Factor Pathway Inhibitor 2 (TFPI2). This antibody is labeled with Biotin. |
Tissue Factor Pathway Inhibitor 2 (TFPI2) Polyclonal Antibody (Mouse), Cy3 |
4-PAB940Mu01-Cy3 |
Cloud-Clone |
-
EUR 411.00
-
EUR 4229.00
-
EUR 1151.00
-
EUR 535.00
-
EUR 248.00
|
-
100ul
-
10ml
-
1ml
-
200ul
-
20ul
|
|
Description: A Rabbit polyclonal antibody against Mouse Tissue Factor Pathway Inhibitor 2 (TFPI2). This antibody is labeled with Cy3. |
Tissue Factor Pathway Inhibitor 2 (TFPI2) Polyclonal Antibody (Mouse), FITC |
4-PAB940Mu01-FITC |
Cloud-Clone |
-
EUR 292.00
-
EUR 2582.00
-
EUR 735.00
-
EUR 366.00
-
EUR 194.00
|
-
100ul
-
10ml
-
1ml
-
200ul
-
20ul
|
|
Description: A Rabbit polyclonal antibody against Mouse Tissue Factor Pathway Inhibitor 2 (TFPI2). This antibody is labeled with FITC. |
Tissue Factor Pathway Inhibitor 2 (TFPI2) Polyclonal Antibody (Mouse), HRP |
4-PAB940Mu01-HRP |
Cloud-Clone |
-
EUR 311.00
-
EUR 2792.00
-
EUR 791.00
-
EUR 391.00
-
EUR 205.00
|
-
100ul
-
10ml
-
1ml
-
200ul
-
20ul
|
|
Description: A Rabbit polyclonal antibody against Mouse Tissue Factor Pathway Inhibitor 2 (TFPI2). This antibody is labeled with HRP. |
Tissue Factor Pathway Inhibitor 2 (TFPI2) Polyclonal Antibody (Mouse), PE |
4-PAB940Mu01-PE |
Cloud-Clone |
-
EUR 292.00
-
EUR 2582.00
-
EUR 735.00
-
EUR 366.00
-
EUR 194.00
|
-
100ul
-
10ml
-
1ml
-
200ul
-
20ul
|
|
Description: A Rabbit polyclonal antibody against Mouse Tissue Factor Pathway Inhibitor 2 (TFPI2). This antibody is labeled with PE. |
Tissue Factor Pathway Inhibitor 2 (TFPI2) Polyclonal Antibody (Rat), APC |
4-PAB940Ra01-APC |
Cloud-Clone |
-
EUR 352.00
-
EUR 3383.00
-
EUR 939.00
-
EUR 450.00
-
EUR 223.00
|
-
100ul
-
10ml
-
1ml
-
200ul
-
20ul
|
|
Description: A Rabbit polyclonal antibody against Rat Tissue Factor Pathway Inhibitor 2 (TFPI2). This antibody is labeled with APC. |
Tissue Factor Pathway Inhibitor 2 (TFPI2) Polyclonal Antibody (Rat), Biotinylated |
4-PAB940Ra01-Biotin |
Cloud-Clone |
-
EUR 316.00
-
EUR 2539.00
-
EUR 747.00
-
EUR 388.00
-
EUR 221.00
|
-
100ul
-
10ml
-
1ml
-
200ul
-
20ul
|
|
Description: A Rabbit polyclonal antibody against Rat Tissue Factor Pathway Inhibitor 2 (TFPI2). This antibody is labeled with Biotin. |
Tissue Factor Pathway Inhibitor 2 (TFPI2) Polyclonal Antibody (Rat), Cy3 |
4-PAB940Ra01-Cy3 |
Cloud-Clone |
-
EUR 428.00
-
EUR 4469.00
-
EUR 1211.00
-
EUR 559.00
-
EUR 255.00
|
-
100ul
-
10ml
-
1ml
-
200ul
-
20ul
|
|
Description: A Rabbit polyclonal antibody against Rat Tissue Factor Pathway Inhibitor 2 (TFPI2). This antibody is labeled with Cy3. |
Tissue Factor Pathway Inhibitor 2 (TFPI2) Polyclonal Antibody (Rat), FITC |
4-PAB940Ra01-FITC |
Cloud-Clone |
-
EUR 302.00
-
EUR 2726.00
-
EUR 771.00
-
EUR 380.00
-
EUR 198.00
|
-
100ul
-
10ml
-
1ml
-
200ul
-
20ul
|
|
Description: A Rabbit polyclonal antibody against Rat Tissue Factor Pathway Inhibitor 2 (TFPI2). This antibody is labeled with FITC. |
Tissue Factor Pathway Inhibitor 2 (TFPI2) Polyclonal Antibody (Rat), HRP |
4-PAB940Ra01-HRP |
Cloud-Clone |
-
EUR 322.00
-
EUR 2948.00
-
EUR 830.00
-
EUR 407.00
-
EUR 210.00
|
-
100ul
-
10ml
-
1ml
-
200ul
-
20ul
|
|
Description: A Rabbit polyclonal antibody against Rat Tissue Factor Pathway Inhibitor 2 (TFPI2). This antibody is labeled with HRP. |
Tissue Factor Pathway Inhibitor 2 (TFPI2) Polyclonal Antibody (Rat), PE |
4-PAB940Ra01-PE |
Cloud-Clone |
-
EUR 302.00
-
EUR 2726.00
-
EUR 771.00
-
EUR 380.00
-
EUR 198.00
|
-
100ul
-
10ml
-
1ml
-
200ul
-
20ul
|
|
Description: A Rabbit polyclonal antibody against Rat Tissue Factor Pathway Inhibitor 2 (TFPI2). This antibody is labeled with PE. |
Guinea pig Tissue Factor Pathway Inhibitor 2 (TFPI2) ELISA Kit |
abx357941-96tests |
Abbexa |
96 tests |
EUR 825 |
|
ELISA kit for Human TFPI2 (Tissue Factor Pathway Inhibitor 2) |
ELK2691 |
ELK Biotech |
1 plate of 96 wells |
EUR 432 |
|
Description: A sandwich ELISA kit for detection of Tissue Factor Pathway Inhibitor 2 from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids. |
TFPI2 ELISA Kit| Mouse Tissue Factor Pathway Inhibitor 2 ELISA K |
EF013927 |
Lifescience Market |
96 Tests |
EUR 689 |
Tissue Factor Pathway Inhibitor 2 (TFPI2) Polyclonal Antibody (Human), APC-Cy7 |
4-PAB940Hu01-APC-Cy7 |
Cloud-Clone |
-
EUR 547.00
-
EUR 6106.00
-
EUR 1624.00
-
EUR 727.00
-
EUR 310.00
|
-
100ul
-
10ml
-
1ml
-
200ul
-
20ul
|
|
Description: A Rabbit polyclonal antibody against Human Tissue Factor Pathway Inhibitor 2 (TFPI2). This antibody is labeled with APC-Cy7. |
Tissue Factor Pathway Inhibitor 2 (TFPI2) Polyclonal Antibody (Mouse), APC-Cy7 |
4-PAB940Mu01-APC-Cy7 |
Cloud-Clone |
-
EUR 560.00
-
EUR 6286.00
-
EUR 1669.00
-
EUR 745.00
-
EUR 315.00
|
-
100ul
-
10ml
-
1ml
-
200ul
-
20ul
|
|
Description: A Rabbit polyclonal antibody against Mouse Tissue Factor Pathway Inhibitor 2 (TFPI2). This antibody is labeled with APC-Cy7. |
Tissue Factor Pathway Inhibitor 2 (TFPI2) Polyclonal Antibody (Rat), APC-Cy7 |
4-PAB940Ra01-APC-Cy7 |
Cloud-Clone |
-
EUR 585.00
-
EUR 6646.00
-
EUR 1759.00
-
EUR 781.00
-
EUR 326.00
|
-
100ul
-
10ml
-
1ml
-
200ul
-
20ul
|
|
Description: A Rabbit polyclonal antibody against Rat Tissue Factor Pathway Inhibitor 2 (TFPI2). This antibody is labeled with APC-Cy7. |
Tissue Factor Pathway Inhibitor 2 Protein |
20-abx263563 |
Abbexa |
-
EUR 230.00
-
EUR 1609.00
-
EUR 328.00
|
|
|
Recombinant Human Tissue Factor Pathway Inhibitor 2 |
7-06643 |
CHI Scientific |
2µg |
Ask for price |
Recombinant Human Tissue Factor Pathway Inhibitor 2 |
7-06644 |
CHI Scientific |
5µg |
Ask for price |
Recombinant Human Tissue Factor Pathway Inhibitor 2 |
7-06645 |
CHI Scientific |
10µg |
Ask for price |
Recombinant Tissue Factor Pathway Inhibitor (TFPI) |
4-RPA394Hu01 |
Cloud-Clone |
-
EUR 476.32
-
EUR 230.00
-
EUR 1511.20
-
EUR 570.40
-
EUR 1040.80
-
EUR 382.00
-
EUR 3628.00
|
-
100 ug
-
10ug
-
1 mg
-
200 ug
-
500 ug
-
50ug
-
5 mg
|
|
Description: Recombinant Human Tissue Factor Pathway Inhibitor expressed in: E.coli |
Recombinant Tissue Factor Pathway Inhibitor (TFPI) |
4-RPA394Hu02 |
Cloud-Clone |
-
EUR 476.32
-
EUR 230.00
-
EUR 1511.20
-
EUR 570.40
-
EUR 1040.80
-
EUR 382.00
-
EUR 3628.00
|
-
100 ug
-
10ug
-
1 mg
-
200 ug
-
500 ug
-
50ug
-
5 mg
|
|
Description: Recombinant Human Tissue Factor Pathway Inhibitor expressed in: E.coli |
Recombinant Tissue Factor Pathway Inhibitor (TFPI) |
4-RPA394Hu03 |
Cloud-Clone |
-
EUR 476.32
-
EUR 230.00
-
EUR 1511.20
-
EUR 570.40
-
EUR 1040.80
-
EUR 382.00
-
EUR 3628.00
|
-
100 ug
-
10ug
-
1 mg
-
200 ug
-
500 ug
-
50ug
-
5 mg
|
|
Description: Recombinant Human Tissue Factor Pathway Inhibitor expressed in: E.coli |
Recombinant Tissue Factor Pathway Inhibitor (TFPI) |
4-RPA394Mu01 |
Cloud-Clone |
-
EUR 413.60
-
EUR 214.00
-
EUR 1276.00
-
EUR 492.00
-
EUR 884.00
-
EUR 340.00
-
EUR 3040.00
|
-
100 ug
-
10ug
-
1 mg
-
200 ug
-
500 ug
-
50ug
-
5 mg
|
|
Description: Recombinant Mouse Tissue Factor Pathway Inhibitor expressed in: E.coli |
Recombinant Tissue Factor Pathway Inhibitor (TFPI) |
4-RPA394Ra01 |
Cloud-Clone |
-
EUR 350.88
-
EUR 197.00
-
EUR 1040.80
-
EUR 413.60
-
EUR 727.20
-
EUR 298.00
-
EUR 2452.00
|
-
100 ug
-
10ug
-
1 mg
-
200 ug
-
500 ug
-
50ug
-
5 mg
|
|
Description: Recombinant Rat Tissue Factor Pathway Inhibitor expressed in: E.coli |
Recombinant Tissue Factor Pathway Inhibitor (TFPI) |
4-RPA394Rb01 |
Cloud-Clone |
-
EUR 512.16
-
EUR 240.00
-
EUR 1645.60
-
EUR 615.20
-
EUR 1130.40
-
EUR 406.00
-
EUR 3964.00
|
-
100 ug
-
10ug
-
1 mg
-
200 ug
-
500 ug
-
50ug
-
5 mg
|
|
Description: Recombinant Rabbit Tissue Factor Pathway Inhibitor expressed in: E.coli |
Rabbit Tissue factor pathway inhibitor (TFPI) |
1-CSB-YP023437RB |
Cusabio |
-
EUR 679.00
-
EUR 335.00
-
EUR 2172.00
-
EUR 1051.00
-
EUR 1442.00
-
EUR 435.00
|
-
100ug
-
10ug
-
1MG
-
200ug
-
500ug
-
50ug
|
|
Description: Recombinant Rabbit Tissue factor pathway inhibitor(TFPI) expressed in Yeast |
Rabbit Tissue factor pathway inhibitor (TFPI) |
1-CSB-BP023437RB |
Cusabio |
-
EUR 840.00
-
EUR 332.00
-
EUR 2170.00
-
EUR 1135.00
-
EUR 1579.00
-
EUR 470.00
|
-
100ug
-
10ug
-
1MG
-
200ug
-
500ug
-
50ug
|
|
Description: Recombinant Rabbit Tissue factor pathway inhibitor(TFPI) expressed in Baculovirus |
Rabbit Tissue factor pathway inhibitor (TFPI) |
1-CSB-MP023437RB |
Cusabio |
-
EUR 389.00
-
EUR 1394.00
-
EUR 563.00
-
EUR 1025.00
|
|
|
Description: Recombinant Rabbit Tissue factor pathway inhibitor(TFPI) expressed in Mammalian cell |
Human Tissue factor pathway inhibitor (TFPI) |
1-CSB-EP023437HU |
Cusabio |
-
EUR 380.00
-
EUR 214.00
-
EUR 1309.00
-
EUR 560.00
-
EUR 873.00
-
EUR 262.00
|
-
100ug
-
10ug
-
1MG
-
200ug
-
500ug
-
50ug
|
|
Description: Recombinant Human Tissue factor pathway inhibitor(TFPI),partial expressed in E.coli |
Rabbit Tissue factor pathway inhibitor (TFPI) |
1-CSB-EP023437RB |
Cusabio |
-
EUR 611.00
-
EUR 309.00
-
EUR 1827.00
-
EUR 939.00
-
EUR 1218.00
-
EUR 397.00
|
-
100ug
-
10ug
-
1MG
-
200ug
-
500ug
-
50ug
|
|
Description: Recombinant Rabbit Tissue factor pathway inhibitor(TFPI) expressed in E.coli |
Tissue Factor Pathway Inhibitor (TFPI) Antibody |
20-abx129517 |
Abbexa |
-
EUR 411.00
-
EUR 133.00
-
EUR 1135.00
-
EUR 551.00
-
EUR 314.00
|
-
100 ug
-
10 ug
-
1 mg
-
200 ug
-
50 ug
|
|
Tissue Factor Pathway Inhibitor (TFPI) Antibody |
20-abx116110 |
Abbexa |
|
|
|
Tissue Factor Pathway Inhibitor (TFPI) Antibody |
20-abx323593 |
Abbexa |
|
|
|
Tissue Factor Pathway Inhibitor (TFPI) Antibody |
abx433382-200ul |
Abbexa |
200 ul |
EUR 384 |
|
Tissue Factor Pathway Inhibitor (TFPI) Protein |
20-abx261170 |
Abbexa |
-
EUR 3418.00
-
EUR 328.00
-
EUR 230.00
|
|
|
Tissue Factor Pathway Inhibitor (TFPI) Antibody |
abx238634-100ug |
Abbexa |
100 ug |
EUR 551 |
|
Tissue Factor Pathway Inhibitor (TFPI) Antibody |
20-abx174804 |
Abbexa |
|
|
|
Tissue Factor Pathway Inhibitor (TFPI) Antibody |
20-abx001368 |
Abbexa |
-
EUR 411.00
-
EUR 592.00
-
EUR 182.00
-
EUR 314.00
|
-
100 ul
-
200 ul
-
20 ul
-
50 ul
|
|
Tissue Factor Pathway Inhibitor (TFPI) Antibody |
20-abx103325 |
Abbexa |
-
EUR 314.00
-
EUR 133.00
-
EUR 829.00
-
EUR 439.00
-
EUR 272.00
|
-
100 ug
-
10 ug
-
1 mg
-
200 ug
-
50 ug
|
|
Tissue Factor Pathway Inhibitor (TFPI) Antibody |
20-abx103326 |
Abbexa |
-
EUR 314.00
-
EUR 133.00
-
EUR 829.00
-
EUR 439.00
-
EUR 272.00
|
-
100 ug
-
10 ug
-
1 mg
-
200 ug
-
50 ug
|
|
Tissue Factor Pathway Inhibitor (TFPI) Antibody |
20-abx103328 |
Abbexa |
-
EUR 425.00
-
EUR 133.00
-
EUR 1177.00
-
EUR 578.00
-
EUR 328.00
|
-
100 ug
-
10 ug
-
1 mg
-
200 ug
-
50 ug
|
|
Tissue Factor Pathway Inhibitor (TFPI) Antibody |
20-abx103329 |
Abbexa |
-
EUR 425.00
-
EUR 133.00
-
EUR 1177.00
-
EUR 578.00
-
EUR 328.00
|
-
100 ug
-
10 ug
-
1 mg
-
200 ug
-
50 ug
|
|
Tissue Factor Pathway Inhibitor (TFPI) Antibody |
20-abx104947 |
Abbexa |
-
EUR 314.00
-
EUR 133.00
-
EUR 829.00
-
EUR 439.00
-
EUR 272.00
|
-
100 ug
-
10 ug
-
1 mg
-
200 ug
-
50 ug
|
|
Tissue Factor Pathway Inhibitor (TFPI) Antibody |
20-abx132084 |
Abbexa |
-
EUR 300.00
-
EUR 704.00
-
EUR 356.00
-
EUR 154.00
-
EUR 244.00
|
-
100 ug
-
1 mg
-
200 ug
-
20 ug
-
50 ug
|
|
Tissue Factor Pathway Inhibitor (TFPI) Antibody |
20-abx132085 |
Abbexa |
-
EUR 328.00
-
EUR 843.00
-
EUR 439.00
-
EUR 154.00
-
EUR 258.00
|
-
100 ug
-
1 mg
-
200 ug
-
20 ug
-
50 ug
|
|
Tissue Factor Pathway Inhibitor (TFPI) Antibody |
20-abx132086 |
Abbexa |
-
EUR 300.00
-
EUR 704.00
-
EUR 356.00
-
EUR 154.00
-
EUR 244.00
|
-
100 ug
-
1 mg
-
200 ug
-
20 ug
-
50 ug
|
|
ELISA kit for Mouse TFPI (Tissue Factor Pathway Inhibitor) |
E-EL-M1162 |
Elabscience Biotech |
1 plate of 96 wells |
EUR 534 |
|
Description: A sandwich ELISA kit for quantitative measurement of Mouse TFPI (Tissue Factor Pathway Inhibitor) in samples from Serum, Plasma, Cell supernatant |
Right here, we current a fast protocol to provide extremely pure and monomeric recombinant Aβ utilizing a one-step ion change purification methodology and to label the peptide utilizing a maleimide dye. The washing, solublisation and purification steps take solely three hours. We additionally current a protocol for the isolation of Aβ-mCherry from mammalian cells.
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