Screening and Production of Recombinant Human Proteins: Protein Production in E. coli

In Chapter 3 , we described the Structural Genomics Consortium (SGC) course of for producing a number of constructs of truncated variations of every protein utilizing LIC. On this chapter we offer a step-by-step process of our E. coli system for take a look at expressing intracellular (soluble) proteins in a 96-well format that allows us to determine which proteins or truncated variations are expressed in a soluble and secure kind appropriate for structural research. As well as, we element the method for scaling up cultures for large-scale protein purification.

This stage of manufacturing is required to acquire enough portions (i.e., milligram quantities) of protein for additional characterization and/or structural research (e.g., crystallization or cryo-EM experiments). Our normal course of is purification by immobilized steel affinity chromatography (IMAC) utilizing nickel resin adopted by dimension exclusion chromatography (SEC), with further procedures arising from the complexity of the protein itself.

Examine of the results of three.1 THz radiation on the expression of recombinant purple fluorescent protein (RFP) in E. coli

Lately, many research have been performed to research the non-thermal results of THz radiation on completely different organisms, however additional research are wanted to totally elucidate the results, particularly on the molecular stage. On this research, we explored the results of at 3.1 THz radiation on protein expression in Escherichia coli (E. coli) utilizing purple fluorescent protein as a reporter molecule.

After Eight hours of steady THz irradiation of micro organism on LB (Luria-Bertani) stable plates at a mean energy of 33 mW/cm² and 10 Hz pulse repetition frequency, we discovered that the plasmid copy quantity, protein expression and fluorescence depth of micro organism from the irradiated space have been 3.8-, 2.7-, and three.

Three occasions greater than in micro organism from the un-irradiated space, respectively. These findings recommend that plasmid replication modified considerably in micro organism uncovered to three.1 THz radiation, leading to elevated protein expression as evidenced by elevated fluorescence depth of the RFP reporter.

Expression of Recombinant Leptospiral Floor Lipoprotein-Lsa27 in E. coli and Its Evaluation for Serodiagnosis of Bovine Leptospirosis by Latex Agglutination Check

The expressed recombinant leptospiral floor adhesion lipoprotein (Lsa27) of pathogenic Leptospira in E. coli was evaluated for the detection of Leptospira antibodies in cattle sera by latex agglutination take a look at (LAT). The Lsa27 missing sign peptide coding gene sequences from L. interrogans serovar Pomona was amplified (~ 660 bp) by PCR and the amplicon was cloned into pETiteN-HisKan vector.

The expressed recombinant Lsa27 histidine-tagged fusion protein (rLsa27) was Ni-NTA affinity purified below denaturation adopted by renaturation strategies. The purified rLsa27 was characterised by SDS-PAGE and immunoblot, which confirmed the leptospiral protein with a MW of ~ 25 kDa. Additional, the ready sensitized latex beads coated with rLsa27 have been evaluated as a diagnostic antigen for detection of pathogenic Leptospira antibodies by utilizing identified microscopic agglutination take a look at (MAT) constructive (n = 74) and detrimental (n = 62) sera for Leptospira antibodies in LAT, which revealed the relative diagnostic sensitivity of 91.89% and specificity of 87.10% in opposition to the gold normal serological take a look at, MAT.

Moreover, on analysis of developed rLsa27 LAT utilizing serum samples from cattle related to the historical past of abortions and reproductive dysfunction (n = 309), the relative sensitivity of 96.15%, and specificity of 89.11% have been noticed. Due to this fact, this fast subject take a look at utilizing the rLsa27 is first of its form and it could possibly be used as a screening take a look at for the detection of Leptospira antibodies or it may be complemented by different diagnostics for the analysis /surveillance of bovine leptospirosis.

E. coli expression and immunological evaluation of expressed recombinant Newcastle illness virus hemagglutinin-neuraminidase protein in chickens

Yearly, the poultry trade experiences important financial losses as a consequence of epidemics of Newcastle illness virus (NDV). Growing new vaccines by figuring out and utilizing the immunogenic hemagglutinin-neuraminidase (HN) protein can defend the poultry trade. Within the current research, the full-length HN protein was expressed in Escherichia coli (E. coli) BL21 (DE3) cells, purified by way of affinity chromatography and detected by way of western blot evaluation utilizing His-specific antibodies.

The purified HN protein was additional evaluated in chickens to review the immune response in opposition to NDV. The profitable manufacturing of HN-specific IgY proved the exercise of the purified HN protein. IgY was current within the serum of immunized chickens. Nonetheless, the immune response was greater in chickens immunized with purified HN protein together with full and incomplete adjuvants than in chickens immunized with solely the HN protein. Key phrases: protein; Newcastle illness virus; poultry; infectious illnesses; vaccines.

Quick purification of recombinant monomeric amyloid-β from E. coli and amyloid-β-mCherry aggregates from mammalian cells

The Alzheimer’s illness associated peptide, Amyloid-beta (Aβ)1-40 and 1-42, has confirmed tough to be purified as a recombinant monomeric protein due its expression in E. coli resulting in the formation of insoluble inclusion our bodies and its tendency to rapidly kind insoluble aggregates.

An enormous array of strategies have been used to date, but many have pitfalls, resembling using tags for ease of Aβ isolation, the formation of Aβ multimers inside the timeframe of extraction or the necessity to reconstitute Aβ from a freeze dried state.

Recombinant Tissue Factor Pathway Inhibitor 2 (TFPI2)
4-RPB940Bo01	Cloud-Clone	EUR 558.75 EUR 253.00 EUR 1820.32 EUR 673.44 EUR 1246.88 EUR 437.00 EUR 4400.80	100 ug 10ug 1 mg 200 ug 500 ug 50ug 5 mg
Description: Recombinant Bovine Tissue Factor Pathway Inhibitor 2 expressed in: E.coli

Recombinant Tissue Factor Pathway Inhibitor 2 (TFPI2)
4-RPB940Hu01	Cloud-Clone	EUR 458.40 EUR 226.00 EUR 1444.00 EUR 548.00 EUR 996.00 EUR 370.00 EUR 3460.00	100 ug 10ug 1 mg 200 ug 500 ug 50ug 5 mg
Description: Recombinant Human Tissue Factor Pathway Inhibitor 2 expressed in: E.coli

Recombinant Tissue Factor Pathway Inhibitor 2 (TFPI2)
4-RPB940Mu01	Cloud-Clone	EUR 503.20 EUR 238.00 EUR 1612.00 EUR 604.00 EUR 1108.00 EUR 400.00 EUR 3880.00	100 ug 10ug 1 mg 200 ug 500 ug 50ug 5 mg
Description: Recombinant Mouse Tissue Factor Pathway Inhibitor 2 expressed in: E.coli

Recombinant Tissue Factor Pathway Inhibitor 2 (TFPI2)
4-RPB940Ra01	Cloud-Clone	EUR 548.00 EUR 250.00 EUR 1780.00 EUR 660.00 EUR 1220.00 EUR 430.00 EUR 4300.00	100 ug 10ug 1 mg 200 ug 500 ug 50ug 5 mg
Description: Recombinant Rat Tissue Factor Pathway Inhibitor 2 expressed in: E.coli

Tissue Factor Pathway Inhibitor 2 (TFPI2) Antibody
abx122715-100ug	Abbexa	100 ug	EUR 391

Tissue Factor Pathway Inhibitor 2 (TFPI2) Antibody
20-abx320289	Abbexa	EUR 300.00 EUR 244.00	100 ul 50 ul

Tissue Factor Pathway Inhibitor 2 (TFPI2) Antibody
20-abx322259	Abbexa	EUR 300.00 EUR 244.00	100 ul 50 ul

Tissue Factor Pathway Inhibitor 2 (TFPI2) Antibody
20-abx323594	Abbexa	EUR 314.00 EUR 244.00	100 ug 50 ug

Tissue Factor Pathway Inhibitor 2 (TFPI2) Antibody
20-abx339482	Abbexa	EUR 411.00 EUR 300.00	100 ul 50 ul

Tissue Factor Pathway Inhibitor 2 (TFPI2) Antibody
20-abx103659	Abbexa	EUR 411.00 EUR 133.00 EUR 1149.00 EUR 565.00 EUR 314.00	100 ug 10 ug 1 mg 200 ug 50 ug

Tissue Factor Pathway Inhibitor 2 (TFPI2) Antibody
20-abx103660	Abbexa	EUR 425.00 EUR 133.00 EUR 1177.00 EUR 578.00 EUR 328.00	100 ug 10 ug 1 mg 200 ug 50 ug

Tissue Factor Pathway Inhibitor 2 (TFPI2) Antibody
20-abx103661	Abbexa	EUR 439.00 EUR 133.00 EUR 1247.00 EUR 592.00 EUR 328.00	100 ug 10 ug 1 mg 200 ug 50 ug

Tissue Factor Pathway Inhibitor 2 (TFPI2) Antibody
20-abx103662	Abbexa	EUR 467.00 EUR 133.00 EUR 1344.00 EUR 634.00 EUR 356.00	100 ug 10 ug 1 mg 200 ug 50 ug

Tissue Factor Pathway Inhibitor 2 (TFPI2) Antibody
20-abx006327	Abbexa	EUR 411.00 EUR 592.00 EUR 182.00 EUR 314.00	100 ul 200 ul 20 ul 50 ul

Tissue Factor Pathway Inhibitor 2 (TFPI2) Antibody
20-abx210903	Abbexa	EUR 411.00 EUR 300.00	100 ul 50 ul

Tissue Factor Pathway Inhibitor 2 (TFPI2) Antibody
20-abx174805	Abbexa	EUR 815.00 EUR 425.00	1 mg 200 ug

ELISA kit for Human TFPI2 (Tissue Factor Pathway Inhibitor 2)
E-EL-H2366	Elabscience Biotech	1 plate of 96 wells	EUR 534
Description: A sandwich ELISA kit for quantitative measurement of Human TFPI2 (Tissue Factor Pathway Inhibitor 2) in samples from Serum, Plasma, Cell supernatant

ELISA kit for Mouse TFPI2 (Tissue Factor Pathway Inhibitor 2)
E-EL-M2641	Elabscience Biotech	1 plate of 96 wells	EUR 534
Description: A sandwich ELISA kit for quantitative measurement of Mouse TFPI2 (Tissue Factor Pathway Inhibitor 2) in samples from Serum, Plasma, Cell supernatant

CLIA kit for Human TFPI2 (Tissue Factor Pathway Inhibitor 2)
E-CL-H1375	Elabscience Biotech	1 plate of 96 wells	EUR 584
Description: A sandwich CLIA kit for quantitative measurement of Human TFPI2 (Tissue Factor Pathway Inhibitor 2) in samples from Serum, Plasma, Cell supernatant

Tissue Factor Pathway Inhibitor 2 (TFPI2) Polyclonal Antibody (Bovine), APC
4-PAB940Bo01-APC	Cloud-Clone	EUR 376.00 EUR 3707.00 EUR 1020.00 EUR 483.00 EUR 232.00	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Bovine Tissue Factor Pathway Inhibitor 2 (TFPI2). This antibody is labeled with APC.

Tissue Factor Pathway Inhibitor 2 (TFPI2) Polyclonal Antibody (Bovine), Biotinylated
4-PAB940Bo01-Biotin	Cloud-Clone	EUR 334.00 EUR 2776.00 EUR 806.00 EUR 412.00 EUR 228.00	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Bovine Tissue Factor Pathway Inhibitor 2 (TFPI2). This antibody is labeled with Biotin.

Tissue Factor Pathway Inhibitor 2 (TFPI2) Polyclonal Antibody (Bovine), Cy3
4-PAB940Bo01-Cy3	Cloud-Clone	EUR 459.00 EUR 4901.00 EUR 1319.00 EUR 602.00 EUR 267.00	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Bovine Tissue Factor Pathway Inhibitor 2 (TFPI2). This antibody is labeled with Cy3.

Tissue Factor Pathway Inhibitor 2 (TFPI2) Polyclonal Antibody (Bovine), FITC
4-PAB940Bo01-FITC	Cloud-Clone	EUR 320.00 EUR 2985.00 EUR 836.00 EUR 406.00 EUR 205.00	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Bovine Tissue Factor Pathway Inhibitor 2 (TFPI2). This antibody is labeled with FITC.

Tissue Factor Pathway Inhibitor 2 (TFPI2) Polyclonal Antibody (Bovine), HRP
4-PAB940Bo01-HRP	Cloud-Clone	EUR 342.00 EUR 3229.00 EUR 901.00 EUR 435.00 EUR 217.00	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Bovine Tissue Factor Pathway Inhibitor 2 (TFPI2). This antibody is labeled with HRP.

Tissue Factor Pathway Inhibitor 2 (TFPI2) Polyclonal Antibody (Bovine), PE
4-PAB940Bo01-PE	Cloud-Clone	EUR 320.00 EUR 2985.00 EUR 836.00 EUR 406.00 EUR 205.00	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Bovine Tissue Factor Pathway Inhibitor 2 (TFPI2). This antibody is labeled with PE.

Human Tissue Factor Pathway Inhibitor 2 (TFPI2) Protein
20-abx069324	Abbexa	EUR 648.00 EUR 272.00 EUR 1943.00 EUR 759.00 EUR 467.00	100 ug 10 ug 1 mg 200 ug 50 ug

Rat Tissue Factor Pathway Inhibitor 2 (TFPI2) Protein
20-abx069325	Abbexa	EUR 759.00 EUR 300.00 EUR 2388.00 EUR 913.00 EUR 537.00	100 ug 10 ug 1 mg 200 ug 50 ug

Mouse Tissue Factor Pathway Inhibitor 2 (TFPI2) Protein
20-abx069326	Abbexa	EUR 704.00 EUR 286.00 EUR 2165.00 EUR 829.00 EUR 495.00	100 ug 10 ug 1 mg 200 ug 50 ug

Cow Tissue Factor Pathway Inhibitor 2 (TFPI2) Protein
20-abx069327	Abbexa	EUR 773.00 EUR 300.00 EUR 2444.00 EUR 926.00 EUR 551.00	100 ug 10 ug 1 mg 200 ug 50 ug

Tissue Factor Pathway Inhibitor 2 (TFPI2) Polyclonal Antibody (Bovine), APC-Cy7
4-PAB940Bo01-APC-Cy7	Cloud-Clone	EUR 632.00 EUR 7294.00 EUR 1921.00 EUR 846.00 EUR 344.00	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Bovine Tissue Factor Pathway Inhibitor 2 (TFPI2). This antibody is labeled with APC-Cy7.

Tissue Factor Pathway Inhibitor 2 (TFPI2) Polyclonal Antibody (Human)
4-PAB940Hu01	Cloud-Clone	EUR 239.00 EUR 2391.00 EUR 598.00 EUR 299.00 EUR 211.00	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Human Tissue Factor Pathway Inhibitor 2 (TFPI2)

Tissue Factor Pathway Inhibitor 2 (TFPI2) Polyclonal Antibody (Mouse)
4-PAB940Mu01	Cloud-Clone	EUR 243.00 EUR 2457.00 EUR 613.00 EUR 305.00 EUR 212.00	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Mouse Tissue Factor Pathway Inhibitor 2 (TFPI2)

Tissue Factor Pathway Inhibitor 2 (TFPI2) Polyclonal Antibody (Rat)
4-PAB940Ra01	Cloud-Clone	EUR 251.00 EUR 2589.00 EUR 643.00 EUR 317.00 EUR 216.00	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Rat Tissue Factor Pathway Inhibitor 2 (TFPI2)

Human Tissue Factor Pathway Inhibitor 2 (TFPI2) ELISA Kit
SEB940Hu-10x96wellstestplate	Cloud-Clone	10x96-wells test plate	EUR 4502.43
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Tissue Factor Pathway Inhibitor 2 (TFPI2) in Plasma.

Human Tissue Factor Pathway Inhibitor 2 (TFPI2) ELISA Kit
SEB940Hu-1x48wellstestplate	Cloud-Clone	1x48-wells test plate	EUR 458.44
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Tissue Factor Pathway Inhibitor 2 (TFPI2) in Plasma.

Human Tissue Factor Pathway Inhibitor 2 (TFPI2) ELISA Kit
SEB940Hu-1x96wellstestplate	Cloud-Clone	1x96-wells test plate	EUR 612.05
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Tissue Factor Pathway Inhibitor 2 (TFPI2) in Plasma.

Human Tissue Factor Pathway Inhibitor 2 (TFPI2) ELISA Kit
SEB940Hu-5x96wellstestplate	Cloud-Clone	5x96-wells test plate	EUR 2454.23
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Tissue Factor Pathway Inhibitor 2 (TFPI2) in Plasma.

Human Tissue Factor Pathway Inhibitor 2 (TFPI2) ELISA Kit
4-SEB940Hu	Cloud-Clone	EUR 4553.00 EUR 2405.00 EUR 613.00	10 plates of 96 wells 5 plates of 96 wells 1 plate of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Tissue Factor Pathway Inhibitor 2 (TFPI2) in samples from Plasma. with no significant corss-reactivity with analogues from other species.

Mouse Tissue Factor Pathway Inhibitor 2 (TFPI2) ELISA Kit
SEB940Mu-10x96wellstestplate	Cloud-Clone	10x96-wells test plate	EUR 4862.4
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Tissue Factor Pathway Inhibitor 2 (TFPI2) in plasma.

Mouse Tissue Factor Pathway Inhibitor 2 (TFPI2) ELISA Kit
SEB940Mu-1x48wellstestplate	Cloud-Clone	1x48-wells test plate	EUR 488.08
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Tissue Factor Pathway Inhibitor 2 (TFPI2) in plasma.

Mouse Tissue Factor Pathway Inhibitor 2 (TFPI2) ELISA Kit
SEB940Mu-1x96wellstestplate	Cloud-Clone	1x96-wells test plate	EUR 654.4
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Tissue Factor Pathway Inhibitor 2 (TFPI2) in plasma.

Mouse Tissue Factor Pathway Inhibitor 2 (TFPI2) ELISA Kit
SEB940Mu-5x96wellstestplate	Cloud-Clone	5x96-wells test plate	EUR 2644.8
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Tissue Factor Pathway Inhibitor 2 (TFPI2) in plasma.

Mouse Tissue Factor Pathway Inhibitor 2 (TFPI2) ELISA Kit
4-SEB940Mu	Cloud-Clone	EUR 4913.00 EUR 2595.00 EUR 655.00	10 plates of 96 wells 5 plates of 96 wells 1 plate of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Mouse Tissue Factor Pathway Inhibitor 2 (TFPI2) in samples from plasma with no significant corss-reactivity with analogues from other species.

TFPI2 Tissue Factor Pathway Inhibitor 2 Human Recombinant Protein
PROTP48307	BosterBio	Regular: 5ug	EUR 317
Description: TFPI2 Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 199 amino acids (23-213 a.a.) and having a molecular mass of 22.9kDa (Migrates at 18-40kDa on SDS-PAGE under reducing conditions).;TFPI2 is expressed with an 8 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.

Human TFPI2/ Tissue factor pathway inhibitor 2 ELISA Kit
E2853Hu	Sunlong	1 Kit	EUR 571

Mouse Tfpi2/ Tissue factor pathway inhibitor 2 ELISA Kit
E1454Mo	Sunlong	1 Kit	EUR 571

Human Tissue Factor Pathway Inhibitor 2 (TFPI2) ELISA Kit
abx575919-96tests	Abbexa	96 tests	EUR 707

Human Tissue Factor Pathway Inhibitor 2 (TFPI2) CLIA Kit
20-abx493277	Abbexa	EUR 7973.00 EUR 4246.00 EUR 981.00	10 × 96 tests 5 × 96 tests 96 tests

Cow Tissue Factor Pathway Inhibitor 2 (TFPI2) ELISA Kit
abx518897-96tests	Abbexa	96 tests	EUR 911

Mouse Tissue Factor Pathway Inhibitor 2 (TFPI2) ELISA Kit
abx518899-96tests	Abbexa	96 tests	EUR 707

Sheep Tissue Factor Pathway Inhibitor 2 (TFPI2) ELISA Kit
abx364686-96tests	Abbexa	96 tests	EUR 926

Monkey Tissue Factor Pathway Inhibitor 2 (TFPI2) ELISA Kit
abx360338-96tests	Abbexa	96 tests	EUR 825

Pig Tissue Factor Pathway Inhibitor 2 (TFPI2) ELISA Kit
abx362050-96tests	Abbexa	96 tests	EUR 825

Rabbit Tissue Factor Pathway Inhibitor 2 (TFPI2) ELISA Kit
abx363282-96tests	Abbexa	96 tests	EUR 825

Chicken Tissue Factor Pathway Inhibitor 2 (TFPI2) ELISA Kit
abx356836-96tests	Abbexa	96 tests	EUR 825

Mouse Tissue Factor Pathway Inhibitor 2 (TFPI2) ELISA Kit
20-abx585190	Abbexa	EUR 7378.00 EUR 3933.00 EUR 911.00	10 × 96 tests 5 × 96 tests 96 tests

Human Tissue Factor Pathway Inhibitor 2 (TFPI2) ELISA Kit
20-abx153263	Abbexa	EUR 7112.00 EUR 3792.00 EUR 879.00	10 × 96 tests 5 × 96 tests 96 tests

Human Tissue Factor Pathway Inhibitor 2 (TFPI2) CLIA Kit
abx196292-96tests	Abbexa	96 tests	EUR 825

Human Tissue Factor Pathway Inhibitor 2 (TFPI2) ELISA Kit
abx251335-96tests	Abbexa	96 tests	EUR 707

Mouse Tissue Factor Pathway Inhibitor 2 (TFPI2) ELISA Kit
abx254458-96tests	Abbexa	96 tests	EUR 707

Human TFPI2(Tissue factor pathway inhibitor 2) ELISA Kit
EH2010	FN Test	96T	EUR 524.1
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 46.875pg/ml

Mouse TFPI2(Tissue Factor Pathway Inhibitor 2) ELISA Kit
EM1399	FN Test	96T	EUR 524.1
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Mus ;Sensitivity: 0.094 ng/ml

Mouse Tissue factor pathway inhibitor 2, Tfpi2 ELISA KIT
ELI-06238m	Lifescience Market	96 Tests	EUR 865

Human Tissue factor pathway inhibitor 2, TFPI2 ELISA KIT
ELI-06239h	Lifescience Market	96 Tests	EUR 824

Tissue Factor Pathway Inhibitor 2 (TFPI2) Polyclonal Antibody (Human), APC
4-PAB940Hu01-APC	Cloud-Clone	EUR 333.00 EUR 3113.00 EUR 872.00 EUR 423.00 EUR 215.00	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Human Tissue Factor Pathway Inhibitor 2 (TFPI2). This antibody is labeled with APC.

Tissue Factor Pathway Inhibitor 2 (TFPI2) Polyclonal Antibody (Human), Biotinylated
4-PAB940Hu01-Biotin	Cloud-Clone	EUR 303.00 EUR 2341.00 EUR 697.00 EUR 369.00 EUR 216.00	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Human Tissue Factor Pathway Inhibitor 2 (TFPI2). This antibody is labeled with Biotin.

Tissue Factor Pathway Inhibitor 2 (TFPI2) Polyclonal Antibody (Human), Cy3
4-PAB940Hu01-Cy3	Cloud-Clone	EUR 403.00 EUR 4109.00 EUR 1121.00 EUR 523.00 EUR 245.00	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Human Tissue Factor Pathway Inhibitor 2 (TFPI2). This antibody is labeled with Cy3.

Tissue Factor Pathway Inhibitor 2 (TFPI2) Polyclonal Antibody (Human), FITC
4-PAB940Hu01-FITC	Cloud-Clone	EUR 287.00 EUR 2510.00 EUR 717.00 EUR 359.00 EUR 192.00	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Human Tissue Factor Pathway Inhibitor 2 (TFPI2). This antibody is labeled with FITC.

Tissue Factor Pathway Inhibitor 2 (TFPI2) Polyclonal Antibody (Human), HRP
4-PAB940Hu01-HRP	Cloud-Clone	EUR 305.00 EUR 2714.00 EUR 772.00 EUR 383.00 EUR 203.00	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Human Tissue Factor Pathway Inhibitor 2 (TFPI2). This antibody is labeled with HRP.

Tissue Factor Pathway Inhibitor 2 (TFPI2) Polyclonal Antibody (Human), PE
4-PAB940Hu01-PE	Cloud-Clone	EUR 287.00 EUR 2510.00 EUR 717.00 EUR 359.00 EUR 192.00	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Human Tissue Factor Pathway Inhibitor 2 (TFPI2). This antibody is labeled with PE.

Tissue Factor Pathway Inhibitor 2 (TFPI2) Polyclonal Antibody (Mouse), APC
4-PAB940Mu01-APC	Cloud-Clone	EUR 340.00 EUR 3203.00 EUR 894.00 EUR 432.00 EUR 217.00	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Mouse Tissue Factor Pathway Inhibitor 2 (TFPI2). This antibody is labeled with APC.

Tissue Factor Pathway Inhibitor 2 (TFPI2) Polyclonal Antibody (Mouse), Biotinylated
4-PAB940Mu01-Biotin	Cloud-Clone	EUR 307.00 EUR 2407.00 EUR 714.00 EUR 375.00 EUR 217.00	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Mouse Tissue Factor Pathway Inhibitor 2 (TFPI2). This antibody is labeled with Biotin.

Tissue Factor Pathway Inhibitor 2 (TFPI2) Polyclonal Antibody (Mouse), Cy3
4-PAB940Mu01-Cy3	Cloud-Clone	EUR 411.00 EUR 4229.00 EUR 1151.00 EUR 535.00 EUR 248.00	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Mouse Tissue Factor Pathway Inhibitor 2 (TFPI2). This antibody is labeled with Cy3.

Tissue Factor Pathway Inhibitor 2 (TFPI2) Polyclonal Antibody (Mouse), FITC
4-PAB940Mu01-FITC	Cloud-Clone	EUR 292.00 EUR 2582.00 EUR 735.00 EUR 366.00 EUR 194.00	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Mouse Tissue Factor Pathway Inhibitor 2 (TFPI2). This antibody is labeled with FITC.

Tissue Factor Pathway Inhibitor 2 (TFPI2) Polyclonal Antibody (Mouse), HRP
4-PAB940Mu01-HRP	Cloud-Clone	EUR 311.00 EUR 2792.00 EUR 791.00 EUR 391.00 EUR 205.00	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Mouse Tissue Factor Pathway Inhibitor 2 (TFPI2). This antibody is labeled with HRP.

Tissue Factor Pathway Inhibitor 2 (TFPI2) Polyclonal Antibody (Mouse), PE
4-PAB940Mu01-PE	Cloud-Clone	EUR 292.00 EUR 2582.00 EUR 735.00 EUR 366.00 EUR 194.00	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Mouse Tissue Factor Pathway Inhibitor 2 (TFPI2). This antibody is labeled with PE.

Tissue Factor Pathway Inhibitor 2 (TFPI2) Polyclonal Antibody (Rat), APC
4-PAB940Ra01-APC	Cloud-Clone	EUR 352.00 EUR 3383.00 EUR 939.00 EUR 450.00 EUR 223.00	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Rat Tissue Factor Pathway Inhibitor 2 (TFPI2). This antibody is labeled with APC.

Tissue Factor Pathway Inhibitor 2 (TFPI2) Polyclonal Antibody (Rat), Biotinylated
4-PAB940Ra01-Biotin	Cloud-Clone	EUR 316.00 EUR 2539.00 EUR 747.00 EUR 388.00 EUR 221.00	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Rat Tissue Factor Pathway Inhibitor 2 (TFPI2). This antibody is labeled with Biotin.

Tissue Factor Pathway Inhibitor 2 (TFPI2) Polyclonal Antibody (Rat), Cy3
4-PAB940Ra01-Cy3	Cloud-Clone	EUR 428.00 EUR 4469.00 EUR 1211.00 EUR 559.00 EUR 255.00	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Rat Tissue Factor Pathway Inhibitor 2 (TFPI2). This antibody is labeled with Cy3.

Tissue Factor Pathway Inhibitor 2 (TFPI2) Polyclonal Antibody (Rat), FITC
4-PAB940Ra01-FITC	Cloud-Clone	EUR 302.00 EUR 2726.00 EUR 771.00 EUR 380.00 EUR 198.00	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Rat Tissue Factor Pathway Inhibitor 2 (TFPI2). This antibody is labeled with FITC.

Tissue Factor Pathway Inhibitor 2 (TFPI2) Polyclonal Antibody (Rat), HRP
4-PAB940Ra01-HRP	Cloud-Clone	EUR 322.00 EUR 2948.00 EUR 830.00 EUR 407.00 EUR 210.00	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Rat Tissue Factor Pathway Inhibitor 2 (TFPI2). This antibody is labeled with HRP.

Tissue Factor Pathway Inhibitor 2 (TFPI2) Polyclonal Antibody (Rat), PE
4-PAB940Ra01-PE	Cloud-Clone	EUR 302.00 EUR 2726.00 EUR 771.00 EUR 380.00 EUR 198.00	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Rat Tissue Factor Pathway Inhibitor 2 (TFPI2). This antibody is labeled with PE.

ELISA kit for Human TFPI2 (Tissue Factor Pathway Inhibitor 2)
ELK2691	ELK Biotech	1 plate of 96 wells	EUR 432
Description: A sandwich ELISA kit for detection of Tissue Factor Pathway Inhibitor 2 from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids.

Guinea pig Tissue Factor Pathway Inhibitor 2 (TFPI2) ELISA Kit
abx357941-96tests	Abbexa	96 tests	EUR 825

Tissue Factor Pathway Inhibitor 2 (TFPI2) Polyclonal Antibody (Human), APC-Cy7
4-PAB940Hu01-APC-Cy7	Cloud-Clone	EUR 547.00 EUR 6106.00 EUR 1624.00 EUR 727.00 EUR 310.00	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Human Tissue Factor Pathway Inhibitor 2 (TFPI2). This antibody is labeled with APC-Cy7.

Tissue Factor Pathway Inhibitor 2 (TFPI2) Polyclonal Antibody (Mouse), APC-Cy7
4-PAB940Mu01-APC-Cy7	Cloud-Clone	EUR 560.00 EUR 6286.00 EUR 1669.00 EUR 745.00 EUR 315.00	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Mouse Tissue Factor Pathway Inhibitor 2 (TFPI2). This antibody is labeled with APC-Cy7.

Tissue Factor Pathway Inhibitor 2 (TFPI2) Polyclonal Antibody (Rat), APC-Cy7
4-PAB940Ra01-APC-Cy7	Cloud-Clone	EUR 585.00 EUR 6646.00 EUR 1759.00 EUR 781.00 EUR 326.00	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Rat Tissue Factor Pathway Inhibitor 2 (TFPI2). This antibody is labeled with APC-Cy7.

TFPI2 ELISA Kit| Mouse Tissue Factor Pathway Inhibitor 2 ELISA K
EF013927	Lifescience Market	96 Tests	EUR 689

Tissue Factor Pathway Inhibitor 2 Protein
20-abx263563	Abbexa	EUR 230.00 EUR 1609.00 EUR 328.00	1 µg 50 ug 5 ug

Recombinant Human Tissue Factor Pathway Inhibitor 2
7-06643	CHI Scientific	2µg	Ask for price

Recombinant Human Tissue Factor Pathway Inhibitor 2
7-06644	CHI Scientific	5µg	Ask for price

Recombinant Human Tissue Factor Pathway Inhibitor 2
7-06645	CHI Scientific	10µg	Ask for price

ELISA kit for Mouse TFPI (Tissue Factor Pathway Inhibitor)
E-EL-M1162	Elabscience Biotech	1 plate of 96 wells	EUR 534
Description: A sandwich ELISA kit for quantitative measurement of Mouse TFPI (Tissue Factor Pathway Inhibitor) in samples from Serum, Plasma, Cell supernatant

ELISA kit for Monkey TFPI (Tissue Factor Pathway Inhibitor)
E-EL-MK0964	Elabscience Biotech	1 plate of 96 wells	EUR 584
Description: A sandwich ELISA kit for quantitative measurement of Monkey TFPI (Tissue Factor Pathway Inhibitor) in samples from Serum, Plasma, Cell supernatant

CLIA kit for Mouse TFPI (Tissue Factor Pathway Inhibitor)
E-CL-M0650	Elabscience Biotech	1 plate of 96 wells	EUR 584
Description: A sandwich CLIA kit for quantitative measurement of Mouse TFPI (Tissue Factor Pathway Inhibitor) in samples from Serum, Plasma, Cell supernatant

CLIA kit for Rat TFPI (Tissue Factor Pathway Inhibitor)
E-CL-R0651	Elabscience Biotech	1 plate of 96 wells	EUR 584
Description: A sandwich CLIA kit for quantitative measurement of Rat TFPI (Tissue Factor Pathway Inhibitor) in samples from Serum, Plasma, Cell supernatant

ELISA kit for Human TFPI (Tissue Factor Pathway Inhibitor)
E-EL-H0165	Elabscience Biotech	1 plate of 96 wells	EUR 534
Description: A sandwich ELISA kit for quantitative measurement of Human TFPI (Tissue Factor Pathway Inhibitor) in samples from Serum, Plasma, Cell supernatant

ELISA kit for Rat TFPI (Tissue Factor Pathway Inhibitor)
E-EL-R0983	Elabscience Biotech	1 plate of 96 wells	EUR 534
Description: A sandwich ELISA kit for quantitative measurement of Rat TFPI (Tissue Factor Pathway Inhibitor) in samples from Serum, Plasma, Cell supernatant

CLIA kit for Human TFPI (Tissue Factor Pathway Inhibitor)
E-CL-H0152	Elabscience Biotech	1 plate of 96 wells	EUR 584
Description: A sandwich CLIA kit for quantitative measurement of Human TFPI (Tissue Factor Pathway Inhibitor) in samples from Serum, Plasma, Cell supernatant

Recombinant Tissue Factor Pathway Inhibitor (TFPI)
4-RPA394Hu01	Cloud-Clone	EUR 476.32 EUR 230.00 EUR 1511.20 EUR 570.40 EUR 1040.80 EUR 382.00 EUR 3628.00	100 ug 10ug 1 mg 200 ug 500 ug 50ug 5 mg
Description: Recombinant Human Tissue Factor Pathway Inhibitor expressed in: E.coli

Recombinant Tissue Factor Pathway Inhibitor (TFPI)
4-RPA394Hu02	Cloud-Clone	EUR 476.32 EUR 230.00 EUR 1511.20 EUR 570.40 EUR 1040.80 EUR 382.00 EUR 3628.00	100 ug 10ug 1 mg 200 ug 500 ug 50ug 5 mg
Description: Recombinant Human Tissue Factor Pathway Inhibitor expressed in: E.coli

Recombinant Tissue Factor Pathway Inhibitor (TFPI)
4-RPA394Hu03	Cloud-Clone	EUR 476.32 EUR 230.00 EUR 1511.20 EUR 570.40 EUR 1040.80 EUR 382.00 EUR 3628.00	100 ug 10ug 1 mg 200 ug 500 ug 50ug 5 mg
Description: Recombinant Human Tissue Factor Pathway Inhibitor expressed in: E.coli

Recombinant Tissue Factor Pathway Inhibitor (TFPI)
4-RPA394Mu01	Cloud-Clone	EUR 413.60 EUR 214.00 EUR 1276.00 EUR 492.00 EUR 884.00 EUR 340.00 EUR 3040.00	100 ug 10ug 1 mg 200 ug 500 ug 50ug 5 mg
Description: Recombinant Mouse Tissue Factor Pathway Inhibitor expressed in: E.coli

Recombinant Tissue Factor Pathway Inhibitor (TFPI)
4-RPA394Ra01	Cloud-Clone	EUR 350.88 EUR 197.00 EUR 1040.80 EUR 413.60 EUR 727.20 EUR 298.00 EUR 2452.00	100 ug 10ug 1 mg 200 ug 500 ug 50ug 5 mg
Description: Recombinant Rat Tissue Factor Pathway Inhibitor expressed in: E.coli

Recombinant Tissue Factor Pathway Inhibitor (TFPI)
4-RPA394Rb01	Cloud-Clone	EUR 512.16 EUR 240.00 EUR 1645.60 EUR 615.20 EUR 1130.40 EUR 406.00 EUR 3964.00	100 ug 10ug 1 mg 200 ug 500 ug 50ug 5 mg
Description: Recombinant Rabbit Tissue Factor Pathway Inhibitor expressed in: E.coli

Rabbit Tissue factor pathway inhibitor (TFPI)
1-CSB-YP023437RB	Cusabio	EUR 679.00 EUR 335.00 EUR 2172.00 EUR 1051.00 EUR 1442.00 EUR 435.00	100ug 10ug 1MG 200ug 500ug 50ug
Description: Recombinant Rabbit Tissue factor pathway inhibitor(TFPI) expressed in Yeast

Rabbit Tissue factor pathway inhibitor (TFPI)
1-CSB-BP023437RB	Cusabio	EUR 840.00 EUR 332.00 EUR 2170.00 EUR 1135.00 EUR 1579.00 EUR 470.00	100ug 10ug 1MG 200ug 500ug 50ug
Description: Recombinant Rabbit Tissue factor pathway inhibitor(TFPI) expressed in Baculovirus

Rabbit Tissue factor pathway inhibitor (TFPI)
1-CSB-MP023437RB	Cusabio	EUR 389.00 EUR 1394.00 EUR 563.00 EUR 1025.00	100ug 1MG 200ug 500ug
Description: Recombinant Rabbit Tissue factor pathway inhibitor(TFPI) expressed in Mammalian cell

Human Tissue factor pathway inhibitor (TFPI)
1-CSB-EP023437HU	Cusabio	EUR 380.00 EUR 214.00 EUR 1309.00 EUR 560.00 EUR 873.00 EUR 262.00	100ug 10ug 1MG 200ug 500ug 50ug
Description: Recombinant Human Tissue factor pathway inhibitor(TFPI),partial expressed in E.coli

Rabbit Tissue factor pathway inhibitor (TFPI)
1-CSB-EP023437RB	Cusabio	EUR 611.00 EUR 309.00 EUR 1827.00 EUR 939.00 EUR 1218.00 EUR 397.00	100ug 10ug 1MG 200ug 500ug 50ug
Description: Recombinant Rabbit Tissue factor pathway inhibitor(TFPI) expressed in E.coli

Tissue Factor Pathway Inhibitor (TFPI) Antibody
20-abx129517	Abbexa	EUR 411.00 EUR 133.00 EUR 1135.00 EUR 551.00 EUR 314.00	100 ug 10 ug 1 mg 200 ug 50 ug

Tissue Factor Pathway Inhibitor (TFPI) Antibody
20-abx116110	Abbexa	EUR 732.00 EUR 398.00	150 ul 50 ul

Tissue Factor Pathway Inhibitor (TFPI) Antibody
20-abx323593	Abbexa	EUR 314.00 EUR 244.00	100 ug 50 ug

Tissue Factor Pathway Inhibitor (TFPI) Antibody
20-abx001368	Abbexa	EUR 411.00 EUR 592.00 EUR 182.00 EUR 314.00	100 ul 200 ul 20 ul 50 ul

Tissue Factor Pathway Inhibitor (TFPI) Antibody
20-abx103325	Abbexa	EUR 314.00 EUR 133.00 EUR 829.00 EUR 439.00 EUR 272.00	100 ug 10 ug 1 mg 200 ug 50 ug

Tissue Factor Pathway Inhibitor (TFPI) Antibody
20-abx103326	Abbexa	EUR 314.00 EUR 133.00 EUR 829.00 EUR 439.00 EUR 272.00	100 ug 10 ug 1 mg 200 ug 50 ug

Tissue Factor Pathway Inhibitor (TFPI) Antibody
20-abx103328	Abbexa	EUR 425.00 EUR 133.00 EUR 1177.00 EUR 578.00 EUR 328.00	100 ug 10 ug 1 mg 200 ug 50 ug

Tissue Factor Pathway Inhibitor (TFPI) Antibody
20-abx103329	Abbexa	EUR 425.00 EUR 133.00 EUR 1177.00 EUR 578.00 EUR 328.00	100 ug 10 ug 1 mg 200 ug 50 ug

Tissue Factor Pathway Inhibitor (TFPI) Antibody
20-abx104947	Abbexa	EUR 314.00 EUR 133.00 EUR 829.00 EUR 439.00 EUR 272.00	100 ug 10 ug 1 mg 200 ug 50 ug

Tissue Factor Pathway Inhibitor (TFPI) Antibody
20-abx132084	Abbexa	EUR 300.00 EUR 704.00 EUR 356.00 EUR 154.00 EUR 244.00	100 ug 1 mg 200 ug 20 ug 50 ug

Tissue Factor Pathway Inhibitor (TFPI) Antibody
20-abx132085	Abbexa	EUR 328.00 EUR 843.00 EUR 439.00 EUR 154.00 EUR 258.00	100 ug 1 mg 200 ug 20 ug 50 ug

Right here, we current a fast protocol to provide extremely pure and monomeric recombinant Aβ utilizing a one-step ion change purification methodology and to label the peptide utilizing a maleimide dye. The washing, solublisation and purification steps take solely three hours. We additionally current a protocol for the isolation of Aβ-mCherry from mammalian cells.