Screening and Production of Recombinant Human Proteins: Protein Production in E. coli

In Chapter 3 , we described the Structural Genomics Consortium (SGC) course of for producing a number of constructs of truncated variations of every protein utilizing LIC. On this chapter we offer a step-by-step process of our E. coli system for take a look at expressing intracellular (soluble) proteins in a 96-well format that allows us to determine which proteins or truncated variations are expressed in a soluble and secure kind appropriate for structural research. As well as, we element the method for scaling up cultures for large-scale protein purification.

This stage of manufacturing is required to acquire enough portions (i.e., milligram quantities) of protein for additional characterization and/or structural research (e.g., crystallization or cryo-EM experiments). Our normal course of is purification by immobilized steel affinity chromatography (IMAC) utilizing nickel resin adopted by dimension exclusion chromatography (SEC), with further procedures arising from the complexity of the protein itself.

Examine of the results of three.1 THz radiation on the expression of recombinant purple fluorescent protein (RFP) in E. coli

Lately, many research have been performed to research the non-thermal results of THz radiation on completely different organisms, however additional research are wanted to totally elucidate the results, particularly on the molecular stage. On this research, we explored the results of at 3.1 THz radiation on protein expression in Escherichia coli (E. coli) utilizing purple fluorescent protein as a reporter molecule.

After Eight hours of steady THz irradiation of micro organism on LB (Luria-Bertani) stable plates at a mean energy of 33 mW/cm² and 10 Hz pulse repetition frequency, we discovered that the plasmid copy quantity, protein expression and fluorescence depth of micro organism from the irradiated space have been 3.8-, 2.7-, and three.

Three occasions greater than in micro organism from the un-irradiated space, respectively. These findings recommend that plasmid replication modified considerably in micro organism uncovered to three.1 THz radiation, leading to elevated protein expression as evidenced by elevated fluorescence depth of the RFP reporter.

Expression of Recombinant Leptospiral Floor Lipoprotein-Lsa27 in E. coli and Its Evaluation for Serodiagnosis of Bovine Leptospirosis by Latex Agglutination Check

The expressed recombinant leptospiral floor adhesion lipoprotein (Lsa27) of pathogenic Leptospira in E. coli was evaluated for the detection of Leptospira antibodies in cattle sera by latex agglutination take a look at (LAT). The Lsa27 missing sign peptide coding gene sequences from L. interrogans serovar Pomona was amplified (~ 660 bp) by PCR and the amplicon was cloned into pETiteN-HisKan vector.

The expressed recombinant Lsa27 histidine-tagged fusion protein (rLsa27) was Ni-NTA affinity purified below denaturation adopted by renaturation strategies. The purified rLsa27 was characterised by SDS-PAGE and immunoblot, which confirmed the leptospiral protein with a MW of ~ 25 kDa. Additional, the ready sensitized latex beads coated with rLsa27 have been evaluated as a diagnostic antigen for detection of pathogenic Leptospira antibodies by utilizing identified microscopic agglutination take a look at (MAT) constructive (n = 74) and detrimental (n = 62) sera for Leptospira antibodies in LAT, which revealed the relative diagnostic sensitivity of 91.89% and specificity of 87.10% in opposition to the gold normal serological take a look at, MAT.

Moreover, on analysis of developed rLsa27 LAT utilizing serum samples from cattle related to the historical past of abortions and reproductive dysfunction (n = 309), the relative sensitivity of 96.15%, and specificity of 89.11% have been noticed. Due to this fact, this fast subject take a look at utilizing the rLsa27 is first of its form and it could possibly be used as a screening take a look at for the detection of Leptospira antibodies or it may be complemented by different diagnostics for the analysis /surveillance of bovine leptospirosis.

E. coli expression and immunological evaluation of expressed recombinant Newcastle illness virus hemagglutinin-neuraminidase protein in chickens

Yearly, the poultry trade experiences important financial losses as a consequence of epidemics of Newcastle illness virus (NDV). Growing new vaccines by figuring out and utilizing the immunogenic hemagglutinin-neuraminidase (HN) protein can defend the poultry trade. Within the current research, the full-length HN protein was expressed in Escherichia coli (E. coli) BL21 (DE3) cells, purified by way of affinity chromatography and detected by way of western blot evaluation utilizing His-specific antibodies.

The purified HN protein was additional evaluated in chickens to review the immune response in opposition to NDV. The profitable manufacturing of HN-specific IgY proved the exercise of the purified HN protein. IgY was current within the serum of immunized chickens. Nonetheless, the immune response was greater in chickens immunized with purified HN protein together with full and incomplete adjuvants than in chickens immunized with solely the HN protein. Key phrases: protein; Newcastle illness virus; poultry; infectious illnesses; vaccines.

Quick purification of recombinant monomeric amyloid-β from E. coli and amyloid-β-mCherry aggregates from mammalian cells

The Alzheimer’s illness associated peptide, Amyloid-beta (Aβ)1-40 and 1-42, has confirmed tough to be purified as a recombinant monomeric protein due its expression in E. coli resulting in the formation of insoluble inclusion our bodies and its tendency to rapidly kind insoluble aggregates.

An enormous array of strategies have been used to date, but many have pitfalls, resembling using tags for ease of Aβ isolation, the formation of Aβ multimers inside the timeframe of extraction or the necessity to reconstitute Aβ from a freeze dried state.

Tissue Factor Pathway Inhibitor 2 (TFPI2) Polyclonal Antibody (Bovine)
4-PAB940Bo01	Cloud-Clone	Ask for price Ask for price Ask for price Ask for price Ask for price	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Bovine Tissue Factor Pathway Inhibitor 2 (TFPI2)

Tissue Factor Pathway Inhibitor 2 (TFPI2) Polyclonal Antibody (Bovine), PE
4-PAB940Bo01-PE	Cloud-Clone	Ask for price Ask for price Ask for price Ask for price Ask for price	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Bovine Tissue Factor Pathway Inhibitor 2 (TFPI2). This antibody is labeled with PE.

Tissue Factor Pathway Inhibitor 2 (TFPI2) Polyclonal Antibody (Bovine), APC
4-PAB940Bo01-APC	Cloud-Clone	Ask for price Ask for price Ask for price Ask for price Ask for price	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Bovine Tissue Factor Pathway Inhibitor 2 (TFPI2). This antibody is labeled with APC.

Tissue Factor Pathway Inhibitor 2 (TFPI2) Polyclonal Antibody (Bovine), Cy3
4-PAB940Bo01-Cy3	Cloud-Clone	Ask for price Ask for price Ask for price Ask for price Ask for price	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Bovine Tissue Factor Pathway Inhibitor 2 (TFPI2). This antibody is labeled with Cy3.

Tissue Factor Pathway Inhibitor 2 (TFPI2) Polyclonal Antibody (Bovine), FITC
4-PAB940Bo01-FITC	Cloud-Clone	Ask for price Ask for price Ask for price Ask for price Ask for price	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Bovine Tissue Factor Pathway Inhibitor 2 (TFPI2). This antibody is labeled with FITC.

Tissue Factor Pathway Inhibitor 2 (TFPI2) Polyclonal Antibody (Bovine), HRP
4-PAB940Bo01-HRP	Cloud-Clone	Ask for price Ask for price Ask for price Ask for price Ask for price	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Bovine Tissue Factor Pathway Inhibitor 2 (TFPI2). This antibody is labeled with HRP.

Tissue Factor Pathway Inhibitor 2 (TFPI2)
MBS6508351-01mg	MyBiosource	0.1mg	EUR 845

Tissue Factor Pathway Inhibitor 2 (TFPI2)
MBS6508351-5x01mg	MyBiosource	5x0.1mg	EUR 3660

Tissue Factor Pathway Inhibitor 2 (TFPI2)
MBS6508352-01mg	MyBiosource	0.1mg	EUR 875

Tissue Factor Pathway Inhibitor 2 (TFPI2)
MBS6508352-5x01mg	MyBiosource	5x0.1mg	EUR 3785

Tissue Factor Pathway Inhibitor 2 (TFPI2) Polyclonal Antibody (Bovine), Biotinylated
4-PAB940Bo01-Biotin	Cloud-Clone	Ask for price Ask for price Ask for price Ask for price Ask for price	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Bovine Tissue Factor Pathway Inhibitor 2 (TFPI2). This antibody is labeled with Biotin.

Tissue Factor Pathway Inhibitor 2 (TFPI2) Polyclonal Antibody (Bovine), APC-Cy7
4-PAB940Bo01-APC-Cy7	Cloud-Clone	Ask for price Ask for price Ask for price Ask for price Ask for price	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Bovine Tissue Factor Pathway Inhibitor 2 (TFPI2). This antibody is labeled with APC-Cy7.

Bovine Tissue Factor Pathway Inhibitor 2 (TFPI2) Antibody Pair Kit (with Standard)
MBS2101085-10x96Tests	MyBiosource	10x96Tests	EUR 1750

Bovine Tissue Factor Pathway Inhibitor 2 (TFPI2) Antibody Pair Kit (with Standard)
MBS2101085-10x96TestsMBS2090685AbPairsSupportPack110x96Tests	MyBiosource	10x96Tests+MBS2090685(AbPairsSupportPack1,10x96Tests)	EUR 1875

Bovine Tissue Factor Pathway Inhibitor 2 (TFPI2) Antibody Pair Kit (with Standard)
MBS2101085-5x96Tests	MyBiosource	5x96Tests	EUR 1100

Bovine Tissue Factor Pathway Inhibitor 2 (TFPI2) Antibody Pair Kit (with Standard)
MBS2101085-5x96TestsMBS2090685AbPairsSupportPack15x96Tests	MyBiosource	5x96Tests+MBS2090685(AbPairsSupportPack1,5x96Tests)	EUR 1165

Active Tissue Factor Pathway Inhibitor 2 (TFPI2)
APB940Hu01	Cloud-Clone	10ug	EUR 330

Active Tissue Factor Pathway Inhibitor 2 (TFPI2)
APB940Mu01	Cloud-Clone	10ug	EUR 324

Active Tissue Factor Pathway Inhibitor 2 (TFPI2)
RPU56357-100ug	Biomatik Corporation	100ug	EUR 1282.6

Active Tissue Factor Pathway Inhibitor 2 (TFPI2)
RPU56357-1mg	Biomatik Corporation	1mg	EUR 5054.4

Active Tissue Factor Pathway Inhibitor 2 (TFPI2)
RPU56357-50ug	Biomatik Corporation	50ug	EUR 777.6

Active Tissue Factor Pathway Inhibitor 2 (TFPI2)
RPU56360-100ug	Biomatik Corporation	100ug	EUR 1016.4

Active Tissue Factor Pathway Inhibitor 2 (TFPI2)
RPU56360-1mg	Biomatik Corporation	1mg	EUR 4521.4

Active Tissue Factor Pathway Inhibitor 2 (TFPI2)
RPU56360-50ug	Biomatik Corporation	50ug	EUR 817.3

Active Tissue Factor Pathway Inhibitor 2 (TFPI2)
MBS2122339-001mg	MyBiosource	0.01mg	EUR 250

Active Tissue Factor Pathway Inhibitor 2 (TFPI2)
MBS2122339-005mg	MyBiosource	0.05mg	EUR 530

Active Tissue Factor Pathway Inhibitor 2 (TFPI2)
MBS2122339-01mg	MyBiosource	0.1mg	EUR 815

Active Tissue Factor Pathway Inhibitor 2 (TFPI2)
MBS2122339-02mg	MyBiosource	0.2mg	EUR 1010

Active Tissue Factor Pathway Inhibitor 2 (TFPI2)
MBS2122339-05mg	MyBiosource	0.5mg	EUR 1960

Active Tissue Factor Pathway Inhibitor 2 (TFPI2)
MBS2122340-001mg	MyBiosource	0.01mg	EUR 245

Active Tissue Factor Pathway Inhibitor 2 (TFPI2)
MBS2122340-005mg	MyBiosource	0.05mg	EUR 520

Active Tissue Factor Pathway Inhibitor 2 (TFPI2)
MBS2122340-01mg	MyBiosource	0.1mg	EUR 800

Active Tissue Factor Pathway Inhibitor 2 (TFPI2)
MBS2122340-02mg	MyBiosource	0.2mg	EUR 990

Active Tissue Factor Pathway Inhibitor 2 (TFPI2)
MBS2122340-05mg	MyBiosource	0.5mg	EUR 1925

TFPI2 (untagged)-Human tissue factor pathway inhibitor 2 (TFPI2)
SC321050	Origene Technologies GmbH	10 µg	Ask for price

TFPI2 (untagged)-Human tissue factor pathway inhibitor 2 (TFPI2)
SC116018	Origene Technologies GmbH	10 µg	Ask for price

Tissue Factor Pathway Inhibitor 2 (TFPI2) Antibody
20-abx103659	Abbexa	Ask for price Ask for price Ask for price Ask for price Ask for price	100 ug 10 ug 1 mg 200 ug 50 ug

Tissue Factor Pathway Inhibitor 2 (TFPI2) Antibody
20-abx103660	Abbexa	Ask for price Ask for price Ask for price Ask for price Ask for price	100 ug 10 ug 1 mg 200 ug 50 ug

Tissue Factor Pathway Inhibitor 2 (TFPI2) Antibody
20-abx103661	Abbexa	Ask for price Ask for price Ask for price Ask for price Ask for price	100 ug 10 ug 1 mg 200 ug 50 ug

Tissue Factor Pathway Inhibitor 2 (TFPI2) Antibody
20-abx103662	Abbexa	Ask for price Ask for price Ask for price Ask for price Ask for price	100 ug 10 ug 1 mg 200 ug 50 ug

Tissue Factor Pathway Inhibitor 2 (TFPI2) Antibody
abx122715-100ug	Abbexa	100 ug	EUR 469.2

Tissue Factor Pathway Inhibitor 2 (TFPI2) Antibody
20-abx006327	Abbexa	Ask for price Ask for price Ask for price Ask for price	100 ul 200 ul 20 ul 50 ul

Tissue Factor Pathway Inhibitor 2 (TFPI2) Antibody
20-abx210903	Abbexa	Ask for price Ask for price	100 ul 50 ul

Tissue Factor Pathway Inhibitor 2 (TFPI2) Antibody
20-abx174805	Abbexa	Ask for price Ask for price	1 mg 200 ug

Tissue Factor Pathway Inhibitor 2 (TFPI2) Antibody
20-abx322259	Abbexa	Ask for price Ask for price	100 ul 50 ul

Tissue Factor Pathway Inhibitor 2 (TFPI2) Antibody
20-abx323594	Abbexa	Ask for price Ask for price	100 ug 50 ug

Tissue Factor Pathway Inhibitor 2 (TFPI2) Antibody
20-abx320289	Abbexa	Ask for price Ask for price	100 ul 50 ul

Tissue Factor Pathway Inhibitor 2 (TFPI2) Antibody
20-abx339482	Abbexa	Ask for price Ask for price	100 ul 50 ul

Tissue Factor Pathway Inhibitor 2 (TFPI2) Antibody
abx210903-100l	Abbexa	100 µl	EUR 350

Tissue Factor Pathway Inhibitor 2 (TFPI2) Antibody
abx210903-50l	Abbexa	50 µl	EUR 250

Tissue Factor Pathway Inhibitor 2 (TFPI2) Antibody
abx174805-100g	Abbexa	100 g	EUR 750

Tissue Factor Pathway Inhibitor 2 (TFPI2) Antibody
abx174805-500g	Abbexa	500 g	Ask for price

Tissue Factor Pathway Inhibitor 2 (TFPI2) Antibody
abx122715-100l	Abbexa	100 µl	Ask for price

Tissue Factor Pathway Inhibitor 2 (TFPI2) Antibody
abx122715-20l	Abbexa	20 µl	EUR 337.5

Tissue Factor Pathway Inhibitor 2 (TFPI2) Antibody
abx122715-50l	Abbexa	50 µl	Ask for price

Tissue Factor Pathway Inhibitor 2 (TFPI2) Antibody
abx339482-100g	Abbexa	100 µg	Ask for price

Tissue Factor Pathway Inhibitor 2 (TFPI2) Antibody
abx339482-20g	Abbexa	20 µg	EUR 250

Tissue Factor Pathway Inhibitor 2 (TFPI2) Antibody
abx339482-50g	Abbexa	50 µg	EUR 350

Tissue Factor Pathway Inhibitor 2 (TFPI2) Antibody
abx323594-100g	Abbexa	100 µg	EUR 250

Tissue Factor Pathway Inhibitor 2 (TFPI2) Antibody
abx323594-50g	Abbexa	50 µg	EUR 187.5

Tissue Factor Pathway Inhibitor 2 (TFPI2) Antibody
abx320289-100l	Abbexa	100 µl	EUR 350

Tissue Factor Pathway Inhibitor 2 (TFPI2) Antibody
abx320289-50l	Abbexa	50 µl	EUR 237.5

Tissue Factor Pathway Inhibitor 2 (TFPI2) Antibody
abx344660-100l	Abbexa	100 µl	EUR 350

Tissue Factor Pathway Inhibitor 2 (TFPI2) Antibody
abx344660-50l	Abbexa	50 µl	EUR 250

Tissue Factor Pathway Inhibitor 2 (TFPI2) Antibody
abx375235-96tests	Abbexa	96 tests	EUR 337.5

Tissue Factor Pathway Inhibitor 2 (TFPI2) Antibody
abx322259-100g	Abbexa	100 µg	EUR 350

Tissue Factor Pathway Inhibitor 2 (TFPI2) Antibody
abx322259-50g	Abbexa	50 µg	EUR 237.5

Tissue Factor Pathway Inhibitor 2 (TFPI2) Antibody
abx006327-100g	Abbexa	100 µg	EUR 275

Tissue Factor Pathway Inhibitor 2 (TFPI2) Antibody
abx006327-10g	Abbexa	10 µg	EUR 175

Tissue Factor Pathway Inhibitor 2 (TFPI2) Antibody
abx006327-200g	Abbexa	200 µg	EUR 400

Tissue Factor Pathway Inhibitor 2 (TFPI2) Antibody
abx103659-100g	Abbexa	100 µg	EUR 712.5

Tissue Factor Pathway Inhibitor 2 (TFPI2) Antibody
abx103659-20g	Abbexa	20 µg	EUR 262.5

Tissue Factor Pathway Inhibitor 2 (TFPI2) Antibody
abx103659-50g	Abbexa	50 µg	EUR 325

Tissue Factor Pathway Inhibitor 2 (TFPI2) Antibody
abx103660-100g	Abbexa	100 µg	EUR 725

Tissue Factor Pathway Inhibitor 2 (TFPI2) Antibody
abx103660-20g	Abbexa	20 µg	EUR 262.5

Tissue Factor Pathway Inhibitor 2 (TFPI2) Antibody
abx103660-50g	Abbexa	50 µg	EUR 337.5

Tissue Factor Pathway Inhibitor 2 (TFPI2) Antibody
abx103661-100g	Abbexa	100 µg	EUR 775

Tissue Factor Pathway Inhibitor 2 (TFPI2) Antibody
abx103661-20g	Abbexa	20 µg	EUR 275

Tissue Factor Pathway Inhibitor 2 (TFPI2) Antibody
abx103661-50g	Abbexa	50 µg	EUR 350

Tissue Factor Pathway Inhibitor 2 (TFPI2) Antibody
abx103662-100g	Abbexa	100 µg	EUR 850

Tissue Factor Pathway Inhibitor 2 (TFPI2) Antibody
abx103662-20g	Abbexa	20 µg	EUR 287.5

Tissue Factor Pathway Inhibitor 2 (TFPI2) Antibody
abx103662-50g	Abbexa	50 µg	EUR 375

Recombinant Tissue Factor Pathway Inhibitor 2 (TFPI2)
RPB940Bo01	Cloud-Clone	10ug	EUR 205

Recombinant Tissue Factor Pathway Inhibitor 2 (TFPI2)
4-RPB940Bo01	Cloud-Clone	Ask for price Ask for price Ask for price Ask for price Ask for price Ask for price Ask for price	100 ug 10ug 1 mg 200 ug 500 ug 50ug 5 mg
Description: Recombinant Bovine Tissue Factor Pathway Inhibitor 2 expressed in: E.coli

Recombinant Tissue Factor Pathway Inhibitor 2 (TFPI2)
RPB940Hu01	Cloud-Clone	10ug	EUR 160

Recombinant Tissue Factor Pathway Inhibitor 2 (TFPI2)
4-RPB940Hu01	Cloud-Clone	Ask for price Ask for price Ask for price Ask for price Ask for price Ask for price Ask for price	100 ug 10ug 1 mg 200 ug 500 ug 50ug 5 mg
Description: Recombinant Human Tissue Factor Pathway Inhibitor 2 expressed in: E.coli

Recombinant Tissue Factor Pathway Inhibitor 2 (TFPI2)
RPB940Mu01	Cloud-Clone	10ug	EUR 180

Recombinant Tissue Factor Pathway Inhibitor 2 (TFPI2)
4-RPB940Mu01	Cloud-Clone	Ask for price Ask for price Ask for price Ask for price Ask for price Ask for price Ask for price	100 ug 10ug 1 mg 200 ug 500 ug 50ug 5 mg
Description: Recombinant Mouse Tissue Factor Pathway Inhibitor 2 expressed in: E.coli

Recombinant Tissue Factor Pathway Inhibitor 2 (TFPI2)
RPB940Ra01	Cloud-Clone	10ug	EUR 200

Recombinant Tissue Factor Pathway Inhibitor 2 (TFPI2)
4-RPB940Ra01	Cloud-Clone	Ask for price Ask for price Ask for price Ask for price Ask for price Ask for price Ask for price	100 ug 10ug 1 mg 200 ug 500 ug 50ug 5 mg
Description: Recombinant Rat Tissue Factor Pathway Inhibitor 2 expressed in: E.coli

Recombinant Tissue Factor Pathway Inhibitor 2 (TFPI2)
RPU44034-100ug	Biomatik Corporation	100ug	EUR 492.8

Recombinant Tissue Factor Pathway Inhibitor 2 (TFPI2)
RPU44034-1mg	Biomatik Corporation	1mg	EUR 2184

Recombinant Tissue Factor Pathway Inhibitor 2 (TFPI2)
RPU44034-50ug	Biomatik Corporation	50ug	EUR 396

Recombinant Tissue Factor Pathway Inhibitor 2 (TFPI2)
RPU44035-100ug	Biomatik Corporation	100ug	EUR 616

Recombinant Tissue Factor Pathway Inhibitor 2 (TFPI2)
RPU44035-1mg	Biomatik Corporation	1mg	EUR 2730

Recombinant Tissue Factor Pathway Inhibitor 2 (TFPI2)
RPU44035-50ug	Biomatik Corporation	50ug	EUR 495

Recombinant Tissue Factor Pathway Inhibitor 2 (TFPI2)
RPU44036-100ug	Biomatik Corporation	100ug	EUR 554.4

Recombinant Tissue Factor Pathway Inhibitor 2 (TFPI2)
RPU44036-1mg	Biomatik Corporation	1mg	EUR 2457

Recombinant Tissue Factor Pathway Inhibitor 2 (TFPI2)
RPU44036-50ug	Biomatik Corporation	50ug	EUR 445.5

Recombinant Tissue Factor Pathway Inhibitor 2 (TFPI2)
RPU44037-100ug	Biomatik Corporation	100ug	EUR 630.3

Recombinant Tissue Factor Pathway Inhibitor 2 (TFPI2)
RPU44037-1mg	Biomatik Corporation	1mg	EUR 2795

Recombinant Tissue Factor Pathway Inhibitor 2 (TFPI2)
RPU44037-50ug	Biomatik Corporation	50ug	EUR 507.1

Recombinant Tissue Factor Pathway Inhibitor 2 (TFPI2)
MBS2012122-001mg	MyBiosource	0.01mg	EUR 175

Recombinant Tissue Factor Pathway Inhibitor 2 (TFPI2)
MBS2012122-005mg	MyBiosource	0.05mg	EUR 310

Recombinant Tissue Factor Pathway Inhibitor 2 (TFPI2)
MBS2012122-01mg	MyBiosource	0.1mg	EUR 470

Recombinant Tissue Factor Pathway Inhibitor 2 (TFPI2)
MBS2012122-02mg	MyBiosource	0.2mg	EUR 575

Recombinant Tissue Factor Pathway Inhibitor 2 (TFPI2)
MBS2012122-05mg	MyBiosource	0.5mg	EUR 1090

Recombinant Tissue Factor Pathway Inhibitor 2 (TFPI2)
MBS2012179-001mg	MyBiosource	0.01mg	EUR 185

Recombinant Tissue Factor Pathway Inhibitor 2 (TFPI2)
MBS2012179-005mg	MyBiosource	0.05mg	EUR 340

Recombinant Tissue Factor Pathway Inhibitor 2 (TFPI2)
MBS2012179-01mg	MyBiosource	0.1mg	EUR 515

Recombinant Tissue Factor Pathway Inhibitor 2 (TFPI2)
MBS2012179-02mg	MyBiosource	0.2mg	EUR 630

Recombinant Tissue Factor Pathway Inhibitor 2 (TFPI2)
MBS2012179-05mg	MyBiosource	0.5mg	EUR 1205

Recombinant Tissue Factor Pathway Inhibitor 2 (TFPI2)
MBS2018355-001mg	MyBiosource	0.01mg	EUR 165

Recombinant Tissue Factor Pathway Inhibitor 2 (TFPI2)
MBS2018355-005mg	MyBiosource	0.05mg	EUR 285

Recombinant Tissue Factor Pathway Inhibitor 2 (TFPI2)
MBS2018355-01mg	MyBiosource	0.1mg	EUR 425

Recombinant Tissue Factor Pathway Inhibitor 2 (TFPI2)
MBS2018355-02mg	MyBiosource	0.2mg	EUR 515

Recombinant Tissue Factor Pathway Inhibitor 2 (TFPI2)
MBS2018355-05mg	MyBiosource	0.5mg	EUR 980

Recombinant Tissue Factor Pathway Inhibitor 2 (TFPI2)
MBS2010649-001mg	MyBiosource	0.01mg	EUR 190

Recombinant Tissue Factor Pathway Inhibitor 2 (TFPI2)
MBS2010649-005mg	MyBiosource	0.05mg	EUR 350

Recombinant Tissue Factor Pathway Inhibitor 2 (TFPI2)
MBS2010649-01mg	MyBiosource	0.1mg	EUR 525

Recombinant Tissue Factor Pathway Inhibitor 2 (TFPI2)
MBS2010649-02mg	MyBiosource	0.2mg	EUR 645

Right here, we current a fast protocol to provide extremely pure and monomeric recombinant Aβ utilizing a one-step ion change purification methodology and to label the peptide utilizing a maleimide dye. The washing, solublisation and purification steps take solely three hours. We additionally current a protocol for the isolation of Aβ-mCherry from mammalian cells.