Recombinant production of active microbial transglutaminase in E. coli by using self-cleavable zymogen with mutated propeptide

Microbial transglutaminase from Streptomyces mobaraensis (MTG) has been extensively utilized in meals business and in addition in analysis and medical functions, since it may possibly site-specifically modify proteins by the cross-linking response of glutamine residue and the first amino group. The recombinant expression system of MTG in E. coli supplies higher accessibility for the researchers and thus can promote additional utilization of MTG. Herein, we report manufacturing of lively and soluble MTG in E. coli through the use of a chimeric protein of tobacco etch virus (TEV) protease and MTG zymogen.

A chimera of TEV protease and MTG zymogen with native propeptide resulted in lively MTG contaminated with cleaved propeptide because of the robust interplay between the propeptide and catalytic area of MTG. Introduction of mutations of K9R and Y11A to the propeptide facilitated dissociation of the cleaved propeptide from the catalytic area of MTG and lively MTG with none contamination of the propeptide was obtained. The particular exercise of the lively MTG was 22.7±2.6 U/mg. The profitable expression and purification of lively MTG through the use of the chimera protein of TEV protease and MTG zymogen with mutations within the propeptide can advance using MTG and the researches utilizing MTG mediated cross-linking reactions.

Technology of novel trimeric fragments of human SP-A and SP-D after recombinant soluble expression in E. coli

Surfactant therapy for neonatal respiratory misery syndrome has dramatically improved survival of preterm infants. Nonetheless, this has resulted in a markedly elevated incidence of sequelae corresponding to neonatal persistent inflammatory lung illness. The present surfactant preparations in medical use lack the pure lung defence proteins surfactant proteins (SP)-A and D. These are identified to have anti-inflammatory and anti-infective properties important for sustaining wholesome non-inflamed lungs.

Supplementation of at the moment accessible animal derived surfactant therapeutics with these anti-inflammatory proteins within the first few days of life may stop the event of inflammatory lung illness in untimely infants. Nonetheless, present methods for manufacturing of recombinant variations of SP-A and SP-D require a posh solubilisation and refolding protocol limiting expression at scale for drug improvement. Utilizing a novel solubility tag, we describe the expression and purification of recombinant fragments of human (rfh) SP-A and SP-D utilizing Escherichia coli with out the necessity for refolding.

We obtained a imply (± SD) of 23.3 (± 5.4) mg and 86 mg (± 3.5) per litre yield of rfhSP-A and rfhSP-D, respectively. rfhSP-D was trimeric and 68% sure to a ManNAc-affinity column, giving a closing yield of 57.5 mg/litre of extremely pure protein, considerably increased than the three.Three mg/litre obtained via the usual refolding protocol. Additional optimisation of this novel lab primarily based methodology may doubtlessly make rfhSP-A and rfhSP-D manufacturing extra commercially possible to allow improvement of novel therapeutics for the therapy of lung an infection and irritation.

Expression, solubilization, refolding and closing purification of recombinant proteins as expressed within the type of “classical inclusion our bodies” in E. coli

Escherichia coli has been most generally used for manufacturing of the recombinant proteins. Over-expression of the recombinant proteins is the mainspring of the inclusion our bodies formation. The refolding of those proteins into bioactive types is cumbersome and partly time-consuming. Within the current examine, we reviewed and mentioned most points concerning the restoration of "classical inclusion our bodies" by specializing in our earlier experiences.

Performing correct strategies of expression, solubilization, refolding and closing purification of those proteins, would make it attainable to get well increased quantities of pro-teins into the native type with applicable conformation. Usually, offering gentle circumstances and correct refolding buffers, would result in get well greater than 40% of inclusion our bodies into bioactive and native conformation.

Efficient Course of Growth for Excessive-level Manufacturing, Purification, Formulation, and Characterization of Recombinant Mecasermin in E. coli

Over-production recombinant Mecasermin was achieved by investigation impact of three components, temperature, inducer quantity and tradition media in three ranges in accordance with Taguchi statistical design in E. coli in a bench-scale bioreactor. In optimum circumstances (induction temperature 28°C, TB+G medium, with 0.1 mM IPTG as inducer) 0.84 g/L Mecasermin with expression ranges of 38% of whole protein and 4.13 g/L closing dry cell biomass was produced, that is likely one of the highest values recombinant protein has been reported within the batch system.

The cell disruption was executed by lysozyme pretreatment with sonication to the environment friendly purification of Mecasermin. The remoted and washed inclusion our bodies had been solubilized in Gdn-HCl at pH 5.Four and folded with glutathione and purified with gel filtration. The purified rhIGF-1 (Mecasermin) was formulated with arginine.

Human Connective Tissue Growth Factor (CTGF) ELISA Kit
DLR-CTGF-Hu-48T	DL Develop	48T	EUR 388
Should the Human Connective Tissue Growth Factor (CTGF) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Human Connective Tissue Growth Factor (CTGF) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Human Connective Tissue Growth Factor (CTGF) ELISA Kit
DLR-CTGF-Hu-96T	DL Develop	96T	EUR 496
Should the Human Connective Tissue Growth Factor (CTGF) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Human Connective Tissue Growth Factor (CTGF) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Mouse Connective Tissue Growth Factor (CTGF) ELISA Kit
DLR-CTGF-Mu-48T	DL Develop	48T	EUR 435
Should the Mouse Connective Tissue Growth Factor (CTGF) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Connective Tissue Growth Factor (CTGF) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Mouse Connective Tissue Growth Factor (CTGF) ELISA Kit
DLR-CTGF-Mu-96T	DL Develop	96T	EUR 561
Should the Mouse Connective Tissue Growth Factor (CTGF) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Connective Tissue Growth Factor (CTGF) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Porcine Connective Tissue Growth Factor (CTGF) ELISA Kit
DLR-CTGF-p-48T	DL Develop	48T	EUR 493
Should the Porcine Connective Tissue Growth Factor (CTGF) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Porcine Connective Tissue Growth Factor (CTGF) in samples from serum, plasma, tissue homogenates or other biological fluids.

Porcine Connective Tissue Growth Factor (CTGF) ELISA Kit
DLR-CTGF-p-96T	DL Develop	96T	EUR 641
Should the Porcine Connective Tissue Growth Factor (CTGF) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Porcine Connective Tissue Growth Factor (CTGF) in samples from serum, plasma, tissue homogenates or other biological fluids.

Rat Connective Tissue Growth Factor (CTGF) ELISA Kit
DLR-CTGF-Ra-48T	DL Develop	48T	EUR 454
Should the Rat Connective Tissue Growth Factor (CTGF) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Rat Connective Tissue Growth Factor (CTGF) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Rat Connective Tissue Growth Factor (CTGF) ELISA Kit
DLR-CTGF-Ra-96T	DL Develop	96T	EUR 587
Should the Rat Connective Tissue Growth Factor (CTGF) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Rat Connective Tissue Growth Factor (CTGF) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Rabbit Connective Tissue Growth Factor (CTGF) ELISA Kit
DLR-CTGF-Rb-48T	DL Develop	48T	EUR 454
Should the Rabbit Connective Tissue Growth Factor (CTGF) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Rabbit Connective Tissue Growth Factor (CTGF) in samples from serum, plasma or other biological fluids.

Rabbit Connective Tissue Growth Factor (CTGF) ELISA Kit
DLR-CTGF-Rb-96T	DL Develop	96T	EUR 587
Should the Rabbit Connective Tissue Growth Factor (CTGF) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Rabbit Connective Tissue Growth Factor (CTGF) in samples from serum, plasma or other biological fluids.

Bovine Connective Tissue Growth Factor (CTGF) ELISA Kit
RDR-CTGF-b-48Tests	Reddot Biotech	48 Tests	EUR 516

Bovine Connective Tissue Growth Factor (CTGF) ELISA Kit
RDR-CTGF-b-96Tests	Reddot Biotech	96 Tests	EUR 716

Chicken Connective Tissue Growth Factor (CTGF) ELISA Kit
RDR-CTGF-Ch-48Tests	Reddot Biotech	48 Tests	EUR 516

Chicken Connective Tissue Growth Factor (CTGF) ELISA Kit
RDR-CTGF-Ch-96Tests	Reddot Biotech	96 Tests	EUR 716

Human Connective Tissue Growth Factor (CTGF) ELISA Kit
RDR-CTGF-Hu-48Tests	Reddot Biotech	48 Tests	EUR 391

Human Connective Tissue Growth Factor (CTGF) ELISA Kit
RDR-CTGF-Hu-96Tests	Reddot Biotech	96 Tests	EUR 538

Mouse Connective Tissue Growth Factor (CTGF) ELISA Kit
RDR-CTGF-Mu-48Tests	Reddot Biotech	48 Tests	EUR 447

Mouse Connective Tissue Growth Factor (CTGF) ELISA Kit
RDR-CTGF-Mu-96Tests	Reddot Biotech	96 Tests	EUR 618

Porcine Connective Tissue Growth Factor (CTGF) ELISA Kit
RDR-CTGF-p-48Tests	Reddot Biotech	48 Tests	EUR 516

Porcine Connective Tissue Growth Factor (CTGF) ELISA Kit
RDR-CTGF-p-96Tests	Reddot Biotech	96 Tests	EUR 716

Rat Connective Tissue Growth Factor (CTGF) ELISA Kit
RDR-CTGF-Ra-48Tests	Reddot Biotech	48 Tests	EUR 470

Rat Connective Tissue Growth Factor (CTGF) ELISA Kit
RDR-CTGF-Ra-96Tests	Reddot Biotech	96 Tests	EUR 651

Rabbit Connective Tissue Growth Factor (CTGF) ELISA Kit
RDR-CTGF-Rb-48Tests	Reddot Biotech	48 Tests	EUR 470

Rabbit Connective Tissue Growth Factor (CTGF) ELISA Kit
RDR-CTGF-Rb-96Tests	Reddot Biotech	96 Tests	EUR 651

Bovine Connective Tissue Growth Factor (CTGF) ELISA Kit
RD-CTGF-b-48Tests	Reddot Biotech	48 Tests	EUR 494

Bovine Connective Tissue Growth Factor (CTGF) ELISA Kit
RD-CTGF-b-96Tests	Reddot Biotech	96 Tests	EUR 684

Human Connective Tissue Growth Factor (CTGF) ELISA Kit
RD-CTGF-Hu-48Tests	Reddot Biotech	48 Tests	EUR 375

Human Connective Tissue Growth Factor (CTGF) ELISA Kit
RD-CTGF-Hu-96Tests	Reddot Biotech	96 Tests	EUR 515

Mouse Connective Tissue Growth Factor (CTGF) ELISA Kit
RD-CTGF-Mu-48Tests	Reddot Biotech	48 Tests	EUR 429

Mouse Connective Tissue Growth Factor (CTGF) ELISA Kit
RD-CTGF-Mu-96Tests	Reddot Biotech	96 Tests	EUR 591

Porcine Connective Tissue Growth Factor (CTGF) ELISA Kit
RD-CTGF-p-48Tests	Reddot Biotech	48 Tests	EUR 494

Porcine Connective Tissue Growth Factor (CTGF) ELISA Kit
RD-CTGF-p-96Tests	Reddot Biotech	96 Tests	EUR 684

Rat Connective Tissue Growth Factor (CTGF) ELISA Kit
RD-CTGF-Ra-48Tests	Reddot Biotech	48 Tests	EUR 450

Rat Connective Tissue Growth Factor (CTGF) ELISA Kit
RD-CTGF-Ra-96Tests	Reddot Biotech	96 Tests	EUR 622

Rabbit Connective Tissue Growth Factor (CTGF) ELISA Kit
RD-CTGF-Rb-48Tests	Reddot Biotech	48 Tests	EUR 450

Rabbit Connective Tissue Growth Factor (CTGF) ELISA Kit
RD-CTGF-Rb-96Tests	Reddot Biotech	96 Tests	EUR 622

Pig Connective Tissue Growth Factor (CTGF) Protein
20-abx066093	Abbexa	EUR 732.00 EUR 286.00 EUR 2305.00 EUR 885.00 EUR 523.00	100 ug 10 ug 1 mg 200 ug 50 ug
Shipped within 5-7 working days.

Human Connective tissue growth factor (CTGF)
1-CSB-EP006147HU	Cusabio	EUR 505.00 EUR 265.00 EUR 1827.00 EUR 766.00 EUR 1218.00 EUR 335.00	100ug 10ug 1MG 200ug 500ug 50ug
MW: 36.3 kDa Buffer composition: Tris-based buffer with 50% glycerol.
Description: Recombinant Human Connective tissue growth factor(CTGF) expressed in E.coli

Human Connective tissue growth factor (CTGF)
1-CSB-EP006147HU1	Cusabio	EUR 380.00 EUR 214.00 EUR 1309.00 EUR 560.00 EUR 873.00 EUR 262.00	100ug 10ug 1MG 200ug 500ug 50ug
MW: 15.1 kDa Buffer composition: Tris-based buffer with 50% glycerol.
Description: Recombinant Human Connective tissue growth factor(CTGF),partial expressed in E.coli

Mouse Connective tissue growth factor (Ctgf)
1-CSB-EP006147MO	Cusabio	EUR 505.00 EUR 265.00 EUR 1827.00 EUR 766.00 EUR 1218.00 EUR 335.00	100ug 10ug 1MG 200ug 500ug 50ug
MW: 53.5 kDa Buffer composition: Tris-based buffer with 50% glycerol.
Description: Recombinant Mouse Connective tissue growth factor (Ctgf),partial expressed in E.coli

Mouse Connective tissue growth factor (Ctgf)
1-CSB-EP006147MOe0	Cusabio	EUR 505.00 EUR 265.00 EUR 1827.00 EUR 766.00 EUR 1218.00 EUR 335.00	100ug 10ug 1MG 200ug 500ug 50ug
MW: 62 kDa Buffer composition: Tris-based buffer with 50% glycerol.
Description: Recombinant Mouse Connective tissue growth factor(Ctgf),partial expressed in E.coli

Active Connective Tissue Growth Factor (CTGF)
4-APA010Ra01	Cloud-Clone	EUR 664.48 EUR 281.00 EUR 2216.80 EUR 805.60 EUR 1511.20 EUR 508.00 EUR 5392.00	100 ug 10ug 1 mg 200 ug 500 ug 50ug 5 mg
Uniprot ID: Q9R1E9 Buffer composition: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300. Form: Freeze-dried powder Predicted Molecular Mass (KD): 36.7kDa Isoelectric Point: 8.2
Description: Recombinant Rat Connective Tissue Growth Factor expressed in: Available from E.coli, Yeast, Baculovirus and Mammalian cells

Connective Tissue Growth Factor (CTGF) Antibody
abx025473-100ul	Abbexa	100 ul	EUR 523
Shipped within 5-10 working days.

Connective Tissue Growth Factor (CTGF) Antibody
abx027387-400ul	Abbexa	400 ul	EUR 523
Shipped within 5-10 working days.

Connective Tissue Growth Factor (CTGF) Antibody
abx027387-80l	Abbexa	80 µl	EUR 286
Shipped within 5-10 working days.

Connective tissue growth factor (CTGF) Antibody
20-abx214776	Abbexa	EUR 411.00 EUR 300.00	100 ul 50 ul
Shipped within 5-10 working days.

Connective Tissue Growth Factor (CTGF) Antibody
20-abx175995	Abbexa	EUR 1135.00 EUR 551.00	1 mg 200 ug
Please enquire.

Connective Tissue Growth Factor (CTGF) Antibody
20-abx175996	Abbexa	EUR 1177.00 EUR 578.00	1 mg 200 ug
Please enquire.

Connective Tissue Growth Factor (CTGF) Antibody
20-abx175997	Abbexa	EUR 1316.00 EUR 634.00	1 mg 200 ug
Please enquire.

Connective Tissue Growth Factor (CTGF) Antibody
20-abx103225	Abbexa	EUR 356.00 EUR 133.00 EUR 996.00 EUR 495.00 EUR 300.00	100 ug 10 ug 1 mg 200 ug 50 ug
Shipped within 5-7 working days.

Connective Tissue Growth Factor (CTGF) Antibody
20-abx103226	Abbexa	EUR 356.00 EUR 133.00 EUR 996.00 EUR 495.00 EUR 300.00	100 ug 10 ug 1 mg 200 ug 50 ug
Shipped within 5-12 working days.

Connective Tissue Growth Factor (CTGF) Antibody
20-abx103227	Abbexa	EUR 300.00 EUR 718.00 EUR 384.00 EUR 154.00 EUR 244.00	100 ug 1 mg 200 ug 20 ug 50 ug
Shipped within 5-7 working days.

Connective Tissue Growth Factor (CTGF) Antibody
20-abx103228	Abbexa	EUR 411.00 EUR 133.00 EUR 1135.00 EUR 551.00 EUR 314.00	100 ug 10 ug 1 mg 200 ug 50 ug
Shipped within 5-7 working days.

Connective Tissue Growth Factor (CTGF) Antibody
20-abx103229	Abbexa	EUR 425.00 EUR 133.00 EUR 1177.00 EUR 578.00 EUR 328.00	100 ug 10 ug 1 mg 200 ug 50 ug
Shipped within 5-12 working days.

Connective Tissue Growth Factor (CTGF) Antibody
20-abx103230	Abbexa	EUR 453.00 EUR 133.00 EUR 1288.00 EUR 620.00 EUR 342.00	100 ug 10 ug 1 mg 200 ug 50 ug
Shipped within 5-12 working days.

Connective Tissue Growth Factor (CTGF) Antibody
20-abx103231	Abbexa	EUR 467.00 EUR 133.00 EUR 1372.00 EUR 648.00 EUR 356.00	100 ug 10 ug 1 mg 200 ug 50 ug
Shipped within 5-7 working days.

Connective Tissue Growth Factor (CTGF) Antibody
20-abx104836	Abbexa	EUR 453.00 EUR 133.00 EUR 1288.00 EUR 620.00 EUR 342.00	100 ug 10 ug 1 mg 200 ug 50 ug
Shipped within 5-7 working days.

Connective Tissue Growth Factor (CTGF) Antibody
20-abx123700	Abbexa	EUR 411.00 EUR 592.00	100 ul 200 ul
Shipped within 5-10 working days.

Connective Tissue Growth Factor (CTGF) Antibody
20-abx125726	Abbexa	EUR 411.00 EUR 592.00 EUR 182.00 EUR 314.00	100 ul 200 ul 20 ul 50 ul
Shipped within 5-10 working days.

Connective Tissue Growth Factor (CTGF) Antibody
20-abx125727	Abbexa	EUR 411.00 EUR 592.00 EUR 182.00 EUR 314.00	100 ul 200 ul 20 ul 50 ul
Shipped within 5-10 working days.

Connective Tissue Growth Factor (CTGF) Antibody
20-abx125728	Abbexa	EUR 411.00 EUR 592.00 EUR 314.00	100 ul 200 ul 50 ul
Shipped within 5-10 working days.

Connective Tissue Growth Factor (CTGF) Antibody
abx037800-100ug	Abbexa	100 ug	EUR 391
Shipped within 5-10 working days.

Connective Tissue Growth Factor (CTGF) Antibody
20-abx131768	Abbexa	EUR 328.00 EUR 843.00 EUR 439.00 EUR 154.00 EUR 258.00	100 ug 1 mg 200 ug 20 ug 50 ug
Shipped within 5-7 working days.

Connective Tissue Growth Factor (CTGF) Antibody
20-abx132193	Abbexa	EUR 425.00 EUR 133.00 EUR 1177.00 EUR 578.00 EUR 328.00	100 ug 10 ug 1 mg 200 ug 50 ug
Shipped within 5-15 working days.

Connective Tissue Growth Factor (CTGF) Antibody
20-abx132350	Abbexa	EUR 328.00 EUR 815.00 EUR 425.00 EUR 154.00 EUR 258.00	100 ug 1 mg 200 ug 20 ug 50 ug
Shipped within 5-7 working days.

Connective Tissue Growth Factor (CTGF) Antibody
20-abx132360	Abbexa	EUR 328.00 EUR 829.00 EUR 425.00 EUR 154.00 EUR 258.00	100 ug 1 mg 200 ug 20 ug 50 ug
Shipped within 5-7 working days.

Connective Tissue Growth Factor (CTGF) Antibody
20-abx109670	Abbexa	EUR 411.00 EUR 1845.00 EUR 599.00 EUR 182.00 EUR 300.00	100 ug 1 mg 200 ug 20 ug 50 ug
Shipped within 5-10 working days.

Connective Tissue Growth Factor (CTGF) Antibody
20-abx171909	Abbexa	EUR 843.00 EUR 439.00	1 mg 200 ug
Please enquire.

Connective Tissue Growth Factor (CTGF) Antibody
20-abx171910	Abbexa	EUR 913.00 EUR 467.00	1 mg 200 ug
Please enquire.

Connective Tissue Growth Factor (CTGF) Antibody
abx033675-400ul	Abbexa	400 ul	EUR 523
Shipped within 5-10 working days.

Connective Tissue Growth Factor (CTGF) Antibody
abx033675-80l	Abbexa	80 µl	EUR 286
Shipped within 5-10 working days.

Connective Tissue Growth Factor (CTGF) Antibody
abx033676-400ul	Abbexa	400 ul	EUR 523
Shipped within 5-10 working days.

Connective Tissue Growth Factor (CTGF) Antibody
abx033676-80l	Abbexa	80 µl	EUR 286
Shipped within 5-10 working days.

Connective Tissue Growth Factor (Ctgf) Antibody
20-abx319302	Abbexa	EUR 411.00 EUR 1845.00 EUR 599.00 EUR 182.00 EUR 300.00	100 ug 1 mg 200 ug 20 ug 50 ug
Shipped within 5-10 working days.

Connective Tissue Growth Factor (CTGF) Antibody
abx433502-200ul	Abbexa	200 ul	EUR 453
Shipped within 1-3 working days.

Connective Tissue Growth Factor (CTGF) Antibody
20-abx225128	Abbexa	EUR 370.00 EUR 606.00 EUR 314.00	100 ul 200 ul 50 ul
Shipped within 5-10 working days.

Connective Tissue Growth Factor (CTGF) Antibody
abx232053-100ug	Abbexa	100 ug	EUR 509
Shipped within 5-12 working days.

Connective Tissue Growth Factor (CTGF) Antibody
abx232054-100ug	Abbexa	100 ug	EUR 509
Shipped within 5-12 working days.

Recombinant Connective Tissue Growth Factor (CTGF)
4-RPA010Bo01	Cloud-Clone	EUR 530.08 EUR 245.00 EUR 1712.80 EUR 637.60 EUR 1175.20 EUR 418.00 EUR 4132.00	100 ug 10ug 1 mg 200 ug 500 ug 50ug 5 mg
Uniprot ID: O18739 Buffer composition: PBS, pH 7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. Form: Freeze-dried powder Predicted Molecular Mass (KD): 29.3kDa Isoelectric Point: 8.6
Description: Recombinant Bovine Connective Tissue Growth Factor expressed in: E.coli

Recombinant Connective Tissue Growth Factor (CTGF)
4-RPA010Hu01	Cloud-Clone	EUR 476.32 EUR 230.00 EUR 1511.20 EUR 570.40 EUR 1040.80 EUR 382.00 EUR 3628.00	100 ug 10ug 1 mg 200 ug 500 ug 50ug 5 mg
Uniprot ID: P29279 Buffer composition: PBS, pH 7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. Form: Freeze-dried powder Predicted Molecular Mass (KD): 13.2kDa Isoelectric Point: Inquire
Description: Recombinant Human Connective Tissue Growth Factor expressed in: E.coli

Recombinant Connective Tissue Growth Factor (CTGF)
4-RPA010Hu02	Cloud-Clone	EUR 476.32 EUR 230.00 EUR 1511.20 EUR 570.40 EUR 1040.80 EUR 382.00 EUR 3628.00	100 ug 10ug 1 mg 200 ug 500 ug 50ug 5 mg
Uniprot ID: P29279 Buffer composition: PBS, pH 7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. Form: Freeze-dried powder Predicted Molecular Mass (KD): 14.3kDa Isoelectric Point: 6.6
Description: Recombinant Human Connective Tissue Growth Factor expressed in: E.coli

Recombinant Connective Tissue Growth Factor (CTGF)
4-RPA010Mu01	Cloud-Clone	EUR 503.20 EUR 238.00 EUR 1612.00 EUR 604.00 EUR 1108.00 EUR 400.00 EUR 3880.00	100 ug 10ug 1 mg 200 ug 500 ug 50ug 5 mg
Uniprot ID: P29268 Buffer composition: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300. Form: Freeze-dried powder Predicted Molecular Mass (KD): 12.9kDa Isoelectric Point: 8.9
Description: Recombinant Mouse Connective Tissue Growth Factor expressed in: E.coli

Recombinant Connective Tissue Growth Factor (CTGF)
4-RPA010Ov01	Cloud-Clone	EUR 556.96 EUR 252.00 EUR 1813.60 EUR 671.20 EUR 1242.40 EUR 436.00 EUR 4384.00	100 ug 10ug 1 mg 200 ug 500 ug 50ug 5 mg
Uniprot ID: C7EDS5 Buffer composition: PBS, pH 7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. Form: Freeze-dried powder Predicted Molecular Mass (KD): 27.0kDa Isoelectric Point: Inquire
Description: Recombinant Sheep Connective Tissue Growth Factor expressed in: E.coli

Recombinant Connective Tissue Growth Factor (CTGF)
4-RPA010Po01	Cloud-Clone	EUR 530.08 EUR 245.00 EUR 1712.80 EUR 637.60 EUR 1175.20 EUR 418.00 EUR 4132.00	100 ug 10ug 1 mg 200 ug 500 ug 50ug 5 mg
Uniprot ID: O19113 Buffer composition: PBS, pH 7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. Form: Freeze-dried powder Predicted Molecular Mass (KD): 27.0kDa Isoelectric Point: Inquire
Description: Recombinant Pig Connective Tissue Growth Factor expressed in: E.coli

Recombinant Connective Tissue Growth Factor (CTGF)
4-RPA010Ra01	Cloud-Clone	EUR 413.60 EUR 214.00 EUR 1276.00 EUR 492.00 EUR 884.00 EUR 340.00 EUR 3040.00	100 ug 10ug 1 mg 200 ug 500 ug 50ug 5 mg
Uniprot ID: Q9R1E9 Buffer composition: PBS, pH 7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. Form: Freeze-dried powder Predicted Molecular Mass (KD): 36.7kDa Isoelectric Point: 8.2
Description: Recombinant Rat Connective Tissue Growth Factor expressed in: E.coli

Pig Connective Tissue Growth Factor (CTGF) CLIA Kit
20-abx190822	Abbexa	EUR 9242.00 EUR 4920.00 EUR 1130.00	10 × 96 tests 5 × 96 tests 96 tests
Shipped within 5-7 working days.

Pig Connective Tissue Growth Factor (CTGF) ELISA Kit
20-abx154906	Abbexa	EUR 7645.00 EUR 4074.00 EUR 942.00	10 × 96 tests 5 × 96 tests 96 tests
Shipped within 5-7 working days.

Pig Connective Tissue Growth Factor (CTGF) ELISA Kit
abx573322-96tests	Abbexa	96 tests	EUR 825
Shipped within 5-12 working days.

Connective Tissue Growth Factor (CTGF) Polyclonal Antibody (Pig)
4-PAA010Po01	Cloud-Clone	EUR 259.00 EUR 2694.00 EUR 667.00 EUR 326.00 EUR 218.00	100ul 10ml 1ml 200ul 20ul
Sequence of the immunogen: CTGF (Ala101~Pro330) Buffer composition: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
Description: A Rabbit polyclonal antibody against Pig Connective Tissue Growth Factor (CTGF)

Pig Connective Tissue Growth Factor (CTGF)CLIA Kit
SCA010Po-10x96wellstestplate	Cloud-Clone	10x96-wells test plate	EUR 6747.36
The Intra-assay Precision is determined when 3 samples with low, middle and high level of Pig Connective Tissue Growth Factor (CTGF) were tested on 3 different plates, 8 replicates in each plate CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: Show more
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Pig Connective Tissue Growth Factor (CTGF) in serum, plasma and other biological fluids.

Pig Connective Tissue Growth Factor (CTGF)CLIA Kit
SCA010Po-1x48wellstestplate	Cloud-Clone	1x48-wells test plate	EUR 643.31
The Intra-assay Precision is determined when 3 samples with low, middle and high level of Pig Connective Tissue Growth Factor (CTGF) were tested on 3 different plates, 8 replicates in each plate CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: Show more
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Pig Connective Tissue Growth Factor (CTGF) in serum, plasma and other biological fluids.

Pig Connective Tissue Growth Factor (CTGF)CLIA Kit
SCA010Po-1x96wellstestplate	Cloud-Clone	1x96-wells test plate	EUR 876.16
The Intra-assay Precision is determined when 3 samples with low, middle and high level of Pig Connective Tissue Growth Factor (CTGF) were tested on 3 different plates, 8 replicates in each plate CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: Show more
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Pig Connective Tissue Growth Factor (CTGF) in serum, plasma and other biological fluids.

Pig Connective Tissue Growth Factor (CTGF)CLIA Kit
SCA010Po-5x96wellstestplate	Cloud-Clone	5x96-wells test plate	EUR 3642.72
The Intra-assay Precision is determined when 3 samples with low, middle and high level of Pig Connective Tissue Growth Factor (CTGF) were tested on 3 different plates, 8 replicates in each plate CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: Show more
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Pig Connective Tissue Growth Factor (CTGF) in serum, plasma and other biological fluids.

Pig Connective Tissue Growth Factor (CTGF) CLIA Kit
4-SCA010Po	Cloud-Clone	EUR 6798.00 EUR 3643.00 EUR 877.00	10 plates of 96 wells 5 plates of 96 wells 1 plate of 96 wells
Known also as Connective Tissue Growth Factor Clia kit. Alternative names of the recognized antigen: CCN2 HCS24 IGFBP8 NOV2 Insulin-Like Growth Factor-Binding Protein 8 Hypertrophic Chondrocyte-Specific Protein 24 CCN Family Member 2
Description: Double-antibody Sandwich chemiluminescent immunoassay for detection of Pig Connective Tissue Growth Factor (CTGF)Serum, plasma and other biological fluids

Pig Connective Tissue Growth Factor (CTGF) ELISA Kit
SEA010Po-10x96wellstestplate	Cloud-Clone	10x96-wells test plate	EUR 5098.02
The Intra-assay Precision is determined when 3 samples with low, middle and high level of Pig Connective Tissue Growth Factor (CTGF) were tested on 3 different plates, 8 replicates in each plate CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: Show more
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Pig Connective Tissue Growth Factor (CTGF) in serum, plasma, tissue homogenates and other biological fluids.

Pig Connective Tissue Growth Factor (CTGF) ELISA Kit
SEA010Po-1x48wellstestplate	Cloud-Clone	1x48-wells test plate	EUR 507.48
The Intra-assay Precision is determined when 3 samples with low, middle and high level of Pig Connective Tissue Growth Factor (CTGF) were tested on 3 different plates, 8 replicates in each plate CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: Show more
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Pig Connective Tissue Growth Factor (CTGF) in serum, plasma, tissue homogenates and other biological fluids.

Pig Connective Tissue Growth Factor (CTGF) ELISA Kit
SEA010Po-1x96wellstestplate	Cloud-Clone	1x96-wells test plate	EUR 682.12
The Intra-assay Precision is determined when 3 samples with low, middle and high level of Pig Connective Tissue Growth Factor (CTGF) were tested on 3 different plates, 8 replicates in each plate CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: Show more
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Pig Connective Tissue Growth Factor (CTGF) in serum, plasma, tissue homogenates and other biological fluids.

Pig Connective Tissue Growth Factor (CTGF) ELISA Kit
SEA010Po-5x96wellstestplate	Cloud-Clone	5x96-wells test plate	EUR 2769.54
The Intra-assay Precision is determined when 3 samples with low, middle and high level of Pig Connective Tissue Growth Factor (CTGF) were tested on 3 different plates, 8 replicates in each plate CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: Show more
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Pig Connective Tissue Growth Factor (CTGF) in serum, plasma, tissue homogenates and other biological fluids.

Pig Connective Tissue Growth Factor (CTGF) ELISA Kit
4-SEA010Po	Cloud-Clone	EUR 5149.00 EUR 2720.00 EUR 683.00	10 plates of 96 wells 5 plates of 96 wells 1 plate of 96 wells
Known also as Connective Tissue Growth Factor elisa. Alternative names of the recognized antigen: CCN2 HCS24 IGFBP8 NOV2 Insulin-Like Growth Factor-Binding Protein 8 Hypertrophic Chondrocyte-Specific Protein 24 CCN Family Member 2
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Pig Connective Tissue Growth Factor (CTGF) in samples from Serum, plasma, tissue homogenates and other biological fluids. with no significant corss-reactivity with analogues from other species.

CLIA kit for Human CTGF (Connective Tissue Growth Factor)
E-CL-H0579	Elabscience Biotech	1 plate of 96 wells	EUR 584
Gentaur's CTGF CLIA kit utilizes the Sandwich- CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human CTGF . Standards or samples are added to the micro CLIA plate wells and combined with the Show more
Description: A sandwich CLIA kit for quantitative measurement of Human CTGF (Connective Tissue Growth Factor) in samples from Serum, Plasma, Cell supernatant

CLIA kit for Mouse CTGF (Connective Tissue Growth Factor)
E-CL-M0220	Elabscience Biotech	1 plate of 96 wells	EUR 584
Gentaur's CTGF CLIA kit utilizes the Sandwich- CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse CTGF . Standards or samples are added to the micro CLIA plate wells and combined with the Show more
Description: A sandwich CLIA kit for quantitative measurement of Mouse CTGF (Connective Tissue Growth Factor) in samples from Serum, Plasma, Cell supernatant

CLIA kit for Rat CTGF (Connective Tissue Growth Factor)
E-CL-R0173	Elabscience Biotech	1 plate of 96 wells	EUR 584
Gentaur's CTGF CLIA kit utilizes the Sandwich- CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat CTGF . Standards or samples are added to the micro CLIA plate wells and combined with the sp Show more
Description: A sandwich CLIA kit for quantitative measurement of Rat CTGF (Connective Tissue Growth Factor) in samples from Serum, Plasma, Cell supernatant

ELISA kit for Human CTGF (Connective Tissue Growth Factor)
E-EL-H0828	Elabscience Biotech	1 plate of 96 wells	EUR 534
Gentaur's CTGF ELISA kit utilizes the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CTGF. Standards or samples are added to the micro ELISA plate wells and combined with th Show more
Description: A sandwich ELISA kit for quantitative measurement of Human CTGF (Connective Tissue Growth Factor) in samples from Serum, Plasma, Cell supernatant

ELISA kit for Mouse CTGF (Connective Tissue Growth Factor)
E-EL-M0340	Elabscience Biotech	1 plate of 96 wells	EUR 534
Gentaur's CTGF ELISA kit utilizes the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse CTGF. Standards or samples are added to the micro ELISA plate wells and combined with th Show more
Description: A sandwich ELISA kit for quantitative measurement of Mouse CTGF (Connective Tissue Growth Factor) in samples from Serum, Plasma, Cell supernatant

ELISA kit for Rat CTGF (Connective Tissue Growth Factor)
E-EL-R0259	Elabscience Biotech	1 plate of 96 wells	EUR 534
Gentaur's CTGF ELISA kit utilizes the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat CTGF. Standards or samples are added to the micro ELISA plate wells and combined with the Show more
Description: A sandwich ELISA kit for quantitative measurement of Rat CTGF (Connective Tissue Growth Factor) in samples from Serum, Plasma, Cell supernatant

Guinea pig Connective Tissue Growth Factor (CTGF) ELISA Kit
abx358103-96tests	Abbexa	96 tests	EUR 825
Shipped within 5-12 working days.

ELISA kit for Pig CTGF (Connective Tissue Growth Factor)
ELK5960	ELK Biotech	1 plate of 96 wells	EUR 526
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Connective Tissue Growth Factor (CTGF). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specifi Show more
Description: A sandwich ELISA kit for detection of Connective Tissue Growth Factor from Pig in samples from blood, serum, plasma, cell culture fluid and other biological fluids.

Connective Tissue Growth Factor (CTGF) Polyclonal Antibody (Pig), APC
4-PAA010Po01-APC	Cloud-Clone	EUR 363.00 EUR 3527.00 EUR 975.00 EUR 465.00 EUR 226.00	100ul 10ml 1ml 200ul 20ul
Sequence of the immunogen: CTGF (Ala101~Pro330) Buffer composition: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
Description: A Rabbit polyclonal antibody against Pig Connective Tissue Growth Factor (CTGF). This antibody is labeled with APC.

Connective Tissue Growth Factor (CTGF) Polyclonal Antibody (Pig), Biotinylated
4-PAA010Po01-Biotin	Cloud-Clone	EUR 324.00 EUR 2644.00 EUR 773.00 EUR 399.00 EUR 224.00	100ul 10ml 1ml 200ul 20ul
Sequence of the immunogen: CTGF (Ala101~Pro330) Buffer composition: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
Description: A Rabbit polyclonal antibody against Pig Connective Tissue Growth Factor (CTGF). This antibody is labeled with Biotin.

Connective Tissue Growth Factor (CTGF) Polyclonal Antibody (Pig), Cy3
4-PAA010Po01-Cy3	Cloud-Clone	EUR 443.00 EUR 4661.00 EUR 1259.00 EUR 578.00 EUR 260.00	100ul 10ml 1ml 200ul 20ul
Sequence of the immunogen: CTGF (Ala101~Pro330) Buffer composition: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
Description: A Rabbit polyclonal antibody against Pig Connective Tissue Growth Factor (CTGF). This antibody is labeled with Cy3.

Connective Tissue Growth Factor (CTGF) Polyclonal Antibody (Pig), FITC
4-PAA010Po01-FITC	Cloud-Clone	EUR 310.00 EUR 2841.00 EUR 800.00 EUR 392.00 EUR 201.00	100ul 10ml 1ml 200ul 20ul
Sequence of the immunogen: CTGF (Ala101~Pro330) Buffer composition: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
Description: A Rabbit polyclonal antibody against Pig Connective Tissue Growth Factor (CTGF). This antibody is labeled with FITC.

Connective Tissue Growth Factor (CTGF) Polyclonal Antibody (Pig), HRP
4-PAA010Po01-HRP	Cloud-Clone	EUR 331.00 EUR 3073.00 EUR 862.00 EUR 419.00 EUR 213.00	100ul 10ml 1ml 200ul 20ul
Sequence of the immunogen: CTGF (Ala101~Pro330) Buffer composition: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
Description: A Rabbit polyclonal antibody against Pig Connective Tissue Growth Factor (CTGF). This antibody is labeled with HRP.

Connective Tissue Growth Factor (CTGF) Polyclonal Antibody (Pig), PE
4-PAA010Po01-PE	Cloud-Clone	EUR 310.00 EUR 2841.00 EUR 800.00 EUR 392.00 EUR 201.00	100ul 10ml 1ml 200ul 20ul
Sequence of the immunogen: CTGF (Ala101~Pro330) Buffer composition: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
Description: A Rabbit polyclonal antibody against Pig Connective Tissue Growth Factor (CTGF). This antibody is labeled with PE.

Human Connective Tissue Growth Factor (CTGF) Antibody
35148-05111	AssayPro	150 ug	EUR 261

Connective tissue growth factor (CTGF) Antibody (Biotin)
20-abx105285	Abbexa	EUR 411.00 EUR 1845.00 EUR 599.00 EUR 182.00 EUR 300.00	100 ug 1 mg 200 ug 20 ug 50 ug
Shipped within 5-10 working days.

Connective tissue growth factor (CTGF) Antibody (FITC)
20-abx106704	Abbexa	EUR 411.00 EUR 1845.00 EUR 599.00 EUR 182.00 EUR 300.00	100 ug 1 mg 200 ug 20 ug 50 ug
Shipped within 5-10 working days.

Connective tissue growth factor (CTGF) Antibody (HRP)
20-abx108121	Abbexa	EUR 411.00 EUR 1845.00 EUR 599.00 EUR 182.00 EUR 300.00	100 ug 1 mg 200 ug 20 ug 50 ug
Shipped within 5-10 working days.

Connective Tissue Growth Factor (CTGF) Antibody Pair
abx117594-1pair5x96wellplates	Abbexa	1 pair (5x96 well plates)	EUR 1010
Shipped within 5-10 working days.

Cow Connective Tissue Growth Factor (CTGF) Protein
20-abx168009	Abbexa	EUR 732.00 EUR 286.00 EUR 2305.00 EUR 885.00 EUR 523.00	100 ug 10 ug 1 mg 200 ug 50 ug
Shipped within 5-7 working days.

Human Connective Tissue Growth Factor (CTGF) Protein
20-abx066091	Abbexa	EUR 662.00 EUR 272.00 EUR 2040.00 EUR 787.00 EUR 481.00	100 ug 10 ug 1 mg 200 ug 50 ug
Shipped within 5-12 working days.

Mouse Connective Tissue Growth Factor (CTGF) Protein
20-abx066092	Abbexa	EUR 704.00 EUR 286.00 EUR 2165.00 EUR 829.00 EUR 495.00	100 ug 10 ug 1 mg 200 ug 50 ug
Shipped within 5-7 working days.

Rat Connective Tissue Growth Factor (CTGF) Protein
20-abx066094	Abbexa	EUR 578.00 EUR 258.00 EUR 1720.00 EUR 690.00 EUR 425.00	100 ug 10 ug 1 mg 200 ug 50 ug
Shipped within 5-7 working days.

Mecasermin protein saved stability at 4°C for as much as 2 years. The quantitative and qualitative management indicated that Mecasermin is expressed appropriately (with out the preliminary methionine by Mass spectrometry), pure (with out endotoxin and different protein impurities), appropriate folding (FTIR, RF-HPLC), monomer type (SEC-HPLC) and lively (bioactivity check). Additionally, the purification outcomes revealed that expression in low temperature outcomes the environment friendly purification of the over-produced Mecasermin with a excessive amount and high quality.