Recombinant production of active microbial transglutaminase in E. coli by using self-cleavable zymogen with mutated propeptide

Microbial transglutaminase from Streptomyces mobaraensis (MTG) has been extensively utilized in meals business and in addition in analysis and medical functions, since it may possibly site-specifically modify proteins by the cross-linking response of glutamine residue and the first amino group. The recombinant expression system of MTG in E. coli supplies higher accessibility for the researchers and thus can promote additional utilization of MTG. Herein, we report manufacturing of lively and soluble MTG in E. coli through the use of a chimeric protein of tobacco etch virus (TEV) protease and MTG zymogen.

A chimera of TEV protease and MTG zymogen with native propeptide resulted in lively MTG contaminated with cleaved propeptide because of the robust interplay between the propeptide and catalytic area of MTG. Introduction of mutations of K9R and Y11A to the propeptide facilitated dissociation of the cleaved propeptide from the catalytic area of MTG and lively MTG with none contamination of the propeptide was obtained. The particular exercise of the lively MTG was 22.7±2.6 U/mg. The profitable expression and purification of lively MTG through the use of the chimera protein of TEV protease and MTG zymogen with mutations within the propeptide can advance using MTG and the researches utilizing MTG mediated cross-linking reactions.

Technology of novel trimeric fragments of human SP-A and SP-D after recombinant soluble expression in E. coli

Surfactant therapy for neonatal respiratory misery syndrome has dramatically improved survival of preterm infants. Nonetheless, this has resulted in a markedly elevated incidence of sequelae corresponding to neonatal persistent inflammatory lung illness. The present surfactant preparations in medical use lack the pure lung defence proteins surfactant proteins (SP)-A and D. These are identified to have anti-inflammatory and anti-infective properties important for sustaining wholesome non-inflamed lungs.

Supplementation of at the moment accessible animal derived surfactant therapeutics with these anti-inflammatory proteins within the first few days of life may stop the event of inflammatory lung illness in untimely infants. Nonetheless, present methods for manufacturing of recombinant variations of SP-A and SP-D require a posh solubilisation and refolding protocol limiting expression at scale for drug improvement. Utilizing a novel solubility tag, we describe the expression and purification of recombinant fragments of human (rfh) SP-A and SP-D utilizing Escherichia coli with out the necessity for refolding.

We obtained a imply (± SD) of 23.3 (± 5.4) mg and 86 mg (± 3.5) per litre yield of rfhSP-A and rfhSP-D, respectively. rfhSP-D was trimeric and 68% sure to a ManNAc-affinity column, giving a closing yield of 57.5 mg/litre of extremely pure protein, considerably increased than the three.Three mg/litre obtained via the usual refolding protocol. Additional optimisation of this novel lab primarily based methodology may doubtlessly make rfhSP-A and rfhSP-D manufacturing extra commercially possible to allow improvement of novel therapeutics for the therapy of lung an infection and irritation.

Expression, solubilization, refolding and closing purification of recombinant proteins as expressed within the type of “classical inclusion our bodies” in E. coli

Escherichia coli has been most generally used for manufacturing of the recombinant proteins. Over-expression of the recombinant proteins is the mainspring of the inclusion our bodies formation. The refolding of those proteins into bioactive types is cumbersome and partly time-consuming. Within the current examine, we reviewed and mentioned most points concerning the restoration of "classical inclusion our bodies" by specializing in our earlier experiences.

Performing correct strategies of expression, solubilization, refolding and closing purification of those proteins, would make it attainable to get well increased quantities of pro-teins into the native type with applicable conformation. Usually, offering gentle circumstances and correct refolding buffers, would result in get well greater than 40% of inclusion our bodies into bioactive and native conformation.

Efficient Course of Growth for Excessive-level Manufacturing, Purification, Formulation, and Characterization of Recombinant Mecasermin in E. coli

Over-production recombinant Mecasermin was achieved by investigation impact of three components, temperature, inducer quantity and tradition media in three ranges in accordance with Taguchi statistical design in E. coli in a bench-scale bioreactor. In optimum circumstances (induction temperature 28°C, TB+G medium, with 0.1 mM IPTG as inducer) 0.84 g/L Mecasermin with expression ranges of 38% of whole protein and 4.13 g/L closing dry cell biomass was produced, that is likely one of the highest values recombinant protein has been reported within the batch system.

The cell disruption was executed by lysozyme pretreatment with sonication to the environment friendly purification of Mecasermin. The remoted and washed inclusion our bodies had been solubilized in Gdn-HCl at pH 5.Four and folded with glutathione and purified with gel filtration. The purified rhIGF-1 (Mecasermin) was formulated with arginine.

Pig Connective Tissue Growth Factor (CTGF) CLIA Kit
EKN50637-96T	Biomatik Corporation	96T	EUR 711.2

Pig Connective Tissue Growth Factor (CTGF) CLIA Kit
EKU09526-48T	Biomatik Corporation	48T	EUR 799.82

Pig Connective Tissue Growth Factor (CTGF) CLIA Kit
EKU09526-5x96T	Biomatik Corporation	5x96T	EUR 5427.35

Pig Connective Tissue Growth Factor (CTGF) CLIA Kit
EKU09526-96T	Biomatik Corporation	96T	EUR 1142.6

Pig Connective Tissue Growth Factor (CTGF)CLIA Kit
SCA010Po-10x96wellstestplate	Cloud-Clone	10x96-wells test plate	EUR 8096.83
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Pig Connective Tissue Growth Factor (CTGF) in serum, plasma and other biological fluids.

Pig Connective Tissue Growth Factor (CTGF)CLIA Kit
SCA010Po-1x48wellstestplate	Cloud-Clone	1x48-wells test plate	EUR 771.97
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Pig Connective Tissue Growth Factor (CTGF) in serum, plasma and other biological fluids.

Pig Connective Tissue Growth Factor (CTGF)CLIA Kit
SCA010Po-1x96wellstestplate	Cloud-Clone	1x96-wells test plate	EUR 1051.39
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Pig Connective Tissue Growth Factor (CTGF) in serum, plasma and other biological fluids.

Pig Connective Tissue Growth Factor (CTGF)CLIA Kit
SCA010Po-5x96wellstestplate	Cloud-Clone	5x96-wells test plate	EUR 4371.26
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Pig Connective Tissue Growth Factor (CTGF) in serum, plasma and other biological fluids.

Pig Connective Tissue Growth Factor (CTGF) CLIA Kit
4-SCA010Po	Cloud-Clone	Ask for price Ask for price Ask for price	1 plate of 96 wells 5 plates of 96 wells 10 plates of 96 wells
Description: Double-antibody Sandwich chemiluminescent immunoassay for detection of Pig Connective Tissue Growth Factor (CTGF)Serum, plasma and other biological fluids

Pig Connective Tissue Growth Factor (CTGF) ELISA Kit
abx573322-96tests	Abbexa	96 tests	EUR 990

Pig Connective Tissue Growth Factor (CTGF) ELISA Kit
20-abx154906	Abbexa	Ask for price Ask for price Ask for price	96 tests 5 × 96 tests 10 × 96 tests

Pig Connective Tissue Growth Factor (CTGF) ELISA Kit
EKU03438-48T	Biomatik Corporation	48T	EUR 600.25

Pig Connective Tissue Growth Factor (CTGF) ELISA Kit
EKU03438-5x96T	Biomatik Corporation	5x96T	EUR 4073.13

Pig Connective Tissue Growth Factor (CTGF) ELISA Kit
EKU03438-96T	Biomatik Corporation	96T	EUR 857.5

Pig Connective Tissue Growth Factor (CTGF) ELISA Kit
EKN44549-48T	Biomatik Corporation	48T	EUR 409.22

Pig Connective Tissue Growth Factor (CTGF) ELISA Kit
EKN44549-5x96T	Biomatik Corporation	5x96T	EUR 2776.85

Pig Connective Tissue Growth Factor (CTGF) ELISA Kit
EKN44549-96T	Biomatik Corporation	96T	EUR 584.6

Pig Connective Tissue Growth Factor (CTGF) ELISA Kit
MBS2700731-10x96StripWells	MyBiosource	10x96-Strip-Wells	EUR 3370

Pig Connective Tissue Growth Factor (CTGF) ELISA Kit
MBS2700731-24StripWells	MyBiosource	24-Strip-Wells	EUR 260

Pig Connective Tissue Growth Factor (CTGF) ELISA Kit
MBS2700731-48StripWells	MyBiosource	48-Strip-Wells	EUR 350

Pig Connective Tissue Growth Factor (CTGF) ELISA Kit
MBS2700731-5x96StripWells	MyBiosource	5x96-Strip-Wells	EUR 1750

Pig Connective Tissue Growth Factor (CTGF) ELISA Kit
MBS2700731-96StripWells	MyBiosource	96-Strip-Wells	EUR 445

Pig Connective tissue growth factor (CTGF) ELISA Kit
MBS282142-10x96StripWells	MyBiosource	10x96-Strip-Wells	EUR 6840

Pig Connective tissue growth factor (CTGF) ELISA Kit
MBS282142-48StripWells	MyBiosource	48-Strip-Wells	EUR 480

Pig Connective tissue growth factor (CTGF) ELISA Kit
MBS282142-5x96StripWells	MyBiosource	5x96-Strip-Wells	EUR 3610

Pig Connective tissue growth factor (CTGF) ELISA Kit
MBS282142-96StripWells	MyBiosource	96-Strip-Wells	EUR 805

Pig Connective Tissue Growth Factor (CTGF) ELISA Kit
SEA010Po-10x96wellstestplate	Cloud-Clone	10x96-wells test plate	EUR 6117.62
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Pig Connective Tissue Growth Factor (CTGF) in serum, plasma, tissue homogenates and other biological fluids.

Pig Connective Tissue Growth Factor (CTGF) ELISA Kit
SEA010Po-1x48wellstestplate	Cloud-Clone	1x48-wells test plate	EUR 608.98
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Pig Connective Tissue Growth Factor (CTGF) in serum, plasma, tissue homogenates and other biological fluids.

Pig Connective Tissue Growth Factor (CTGF) ELISA Kit
SEA010Po-1x96wellstestplate	Cloud-Clone	1x96-wells test plate	EUR 818.54
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Pig Connective Tissue Growth Factor (CTGF) in serum, plasma, tissue homogenates and other biological fluids.

Pig Connective Tissue Growth Factor (CTGF) ELISA Kit
SEA010Po-5x96wellstestplate	Cloud-Clone	5x96-wells test plate	EUR 3323.45
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Pig Connective Tissue Growth Factor (CTGF) in serum, plasma, tissue homogenates and other biological fluids.

Pig Connective Tissue Growth Factor (CTGF) ELISA Kit
4-SEA010Po	Cloud-Clone	Ask for price Ask for price Ask for price	1 plate of 96 wells 5 plates of 96 wells 10 plates of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Pig Connective Tissue Growth Factor (CTGF) in samples from Serum, plasma, tissue homogenates and other biological fluids. with no significant corss-reactivity with analogues from other species.

Pig Connective tissue growth factor (CTGF) ELISA Kit
RK09315	Abclonal	96T	EUR 322.37

Connective Tissue Growth Factor (CTGF)
GWB-B2A544	GenWay Biotech	0.02 mg	Ask for price

Pig CTGF/Connective tissue growth factor ELISA Kit
EK19168	SAB	96Т	EUR 650

Pig CTGF (Connective Tissue Growth Factor) ELISA Kit
MBS8804937-10x96StripWells	MyBiosource	10x96-Strip-Wells	EUR 3130

Pig CTGF (Connective Tissue Growth Factor) ELISA Kit
MBS8804937-48StripWells	MyBiosource	48-Strip-Wells	EUR 415

Pig CTGF (Connective Tissue Growth Factor) ELISA Kit
MBS8804937-5x96StripWells	MyBiosource	5x96-Strip-Wells	EUR 1710

Pig CTGF (Connective Tissue Growth Factor) ELISA Kit
MBS8804937-96StripWells	MyBiosource	96-Strip-Wells	EUR 545

Guinea pig Connective Tissue Growth Factor (CTGF) ELISA Kit
abx358103-96tests	Abbexa	96 tests	EUR 990

Guinea pig Connective Tissue Growth Factor (CTGF) ELISA Kit
EIA05533Gu	Enlibio	each	EUR 520

Guinea pig Connective Tissue Growth Factor (CTGF) ELISA Kit
MBS260840-10x96StripWells	MyBiosource	10x96-Strip-Wells	EUR 3545

Guinea pig Connective Tissue Growth Factor (CTGF) ELISA Kit
MBS260840-48StripWells	MyBiosource	48-Strip-Wells	EUR 310

Guinea pig Connective Tissue Growth Factor (CTGF) ELISA Kit
MBS260840-5x96StripWells	MyBiosource	5x96-Strip-Wells	EUR 1940

Guinea pig Connective Tissue Growth Factor (CTGF) ELISA Kit
MBS260840-96StripWells	MyBiosource	96-Strip-Wells	EUR 475

Guinea pig Connective Tissue Growth Factor (CTGF)ELISA Kit
NSL1253Gp	Sunlong	96T	EUR 528
Description: Sandwich ELISA

Human Connective tissue growth factor (CTGF)
1-CSB-EP006147HU	Cusabio	Ask for price Ask for price Ask for price Ask for price Ask for price Ask for price	10ug 50ug 100ug 200ug 500ug 1MG
Description: Recombinant Human Connective tissue growth factor(CTGF) expressed in E.coli

Human Connective tissue growth factor (CTGF)
1-CSB-EP006147HU1	Cusabio	Ask for price Ask for price Ask for price Ask for price Ask for price Ask for price	10ug 50ug 100ug 200ug 500ug 1MG
Description: Recombinant Human Connective tissue growth factor(CTGF),partial expressed in E.coli

Mouse Connective tissue growth factor (Ctgf)
1-CSB-EP006147MO	Cusabio	Ask for price Ask for price Ask for price Ask for price Ask for price Ask for price	10ug 50ug 100ug 200ug 500ug 1MG
Description: Recombinant Mouse Connective tissue growth factor (Ctgf),partial expressed in E.coli

Mouse Connective tissue growth factor (Ctgf)
1-CSB-EP006147MOe0	Cusabio	Ask for price Ask for price Ask for price Ask for price Ask for price Ask for price	10ug 50ug 100ug 200ug 500ug 1MG
Description: Recombinant Mouse Connective tissue growth factor(Ctgf),partial expressed in E.coli

Connective Tissue Growth Factor (CTGF) ELISA
MBS495568-5x96Tests	MyBiosource	5x96Tests	EUR 5095

Connective Tissue Growth Factor (CTGF) ELISA
MBS495568-96Tests	MyBiosource	96Tests	EUR 1100

Active Connective Tissue Growth Factor (CTGF)
APA010Ra01	Cloud-Clone	10ug	EUR 182

Active Connective Tissue Growth Factor (CTGF)
4-APA010Ra01	Cloud-Clone	Ask for price Ask for price Ask for price Ask for price Ask for price Ask for price Ask for price	10ug 50ug 100 ug 200 ug 500 ug 1 mg 5 mg
Description: Recombinant Rat Connective Tissue Growth Factor expressed in: Available from E.coli, Yeast, Baculovirus and Mammalian cells

Active Connective Tissue Growth Factor (CTGF)
MBS2105046-001mg	MyBiosource	0.01mg	EUR 175

Active Connective Tissue Growth Factor (CTGF)
MBS2105046-005mg	MyBiosource	0.05mg	EUR 315

Active Connective Tissue Growth Factor (CTGF)
MBS2105046-01mg	MyBiosource	0.1mg	EUR 475

Active Connective Tissue Growth Factor (CTGF)
MBS2105046-02mg	MyBiosource	0.2mg	EUR 580

Active Connective Tissue Growth Factor (CTGF)
MBS2105046-05mg	MyBiosource	0.5mg	EUR 1100

Active Connective Tissue Growth Factor (CTGF)
RPU55435-100ug	Biomatik Corporation	100ug	EUR 562.1

Active Connective Tissue Growth Factor (CTGF)
RPU55435-1mg	Biomatik Corporation	1mg	EUR 2489.5

Active Connective Tissue Growth Factor (CTGF)
RPU55435-50ug	Biomatik Corporation	50ug	EUR 451

Connective Tissue Growth Factor (CTGF) Polyclonal Antibody (Pig)
4-PAA010Po01	Cloud-Clone	Ask for price Ask for price Ask for price Ask for price Ask for price	20ul 100ul 200ul 1ml 10ml
Description: A Rabbit polyclonal antibody against Pig Connective Tissue Growth Factor (CTGF)

ELISA kit for Pig CTGF (Connective Tissue Growth Factor)
ELK5960	ELK Biotech	1 plate of 96 wells	EUR 631.2
Description: A sandwich ELISA kit for detection of Connective Tissue Growth Factor from Pig in samples from blood, serum, plasma, cell culture fluid and other biological fluids.

Connective Tissue Growth Factor (CTGF) Antibody
20-abx131768	Abbexa	Ask for price Ask for price Ask for price Ask for price Ask for price	20 ug 50 ug 100 ug 200 ug 1 mg

Connective Tissue Growth Factor (CTGF) Antibody
20-abx132193	Abbexa	Ask for price Ask for price Ask for price Ask for price Ask for price	10 ug 50 ug 100 ug 200 ug 1 mg

Connective Tissue Growth Factor (CTGF) Antibody
20-abx132350	Abbexa	Ask for price Ask for price Ask for price Ask for price Ask for price	20 ug 50 ug 100 ug 200 ug 1 mg

Connective Tissue Growth Factor (CTGF) Antibody
20-abx132360	Abbexa	Ask for price Ask for price Ask for price Ask for price Ask for price	20 ug 50 ug 100 ug 200 ug 1 mg

Connective Tissue Growth Factor (CTGF) Antibody
20-abx104836	Abbexa	Ask for price Ask for price Ask for price Ask for price Ask for price	10 ug 50 ug 100 ug 200 ug 1 mg

Connective Tissue Growth Factor (CTGF) Antibody
20-abx109670	Abbexa	Ask for price Ask for price Ask for price Ask for price Ask for price	20 ug 50 ug 100 ug 200 ug 1 mg

Connective Tissue Growth Factor (CTGF) Antibody
20-abx123700	Abbexa	Ask for price Ask for price	100 ul 200 ul

Connective Tissue Growth Factor (CTGF) Antibody
20-abx125726	Abbexa	Ask for price Ask for price Ask for price Ask for price	20 ul 50 ul 100 ul 200 ul

Connective Tissue Growth Factor (CTGF) Antibody
20-abx125727	Abbexa	Ask for price Ask for price Ask for price Ask for price	20 ul 50 ul 100 ul 200 ul

Connective Tissue Growth Factor (CTGF) Antibody
20-abx125728	Abbexa	Ask for price Ask for price Ask for price	50 ul 100 ul 200 ul

Connective Tissue Growth Factor (CTGF) Antibody
20-abx175995	Abbexa	Ask for price Ask for price	200 ug 1 mg

Connective Tissue Growth Factor (CTGF) Antibody
20-abx175996	Abbexa	Ask for price Ask for price	200 ug 1 mg

Connective Tissue Growth Factor (CTGF) Antibody
20-abx175997	Abbexa	Ask for price Ask for price	200 ug 1 mg

Connective Tissue Growth Factor (CTGF) Antibody
20-abx171909	Abbexa	Ask for price Ask for price	200 ug 1 mg

Connective Tissue Growth Factor (CTGF) Antibody
20-abx171910	Abbexa	Ask for price Ask for price	200 ug 1 mg

Connective Tissue Growth Factor (CTGF) Antibody
20-abx103225	Abbexa	Ask for price Ask for price Ask for price Ask for price Ask for price	10 ug 50 ug 100 ug 200 ug 1 mg

Connective Tissue Growth Factor (CTGF) Antibody
20-abx103226	Abbexa	Ask for price Ask for price Ask for price Ask for price Ask for price	10 ug 50 ug 100 ug 200 ug 1 mg

Connective Tissue Growth Factor (CTGF) Antibody
20-abx103227	Abbexa	Ask for price Ask for price Ask for price Ask for price Ask for price	20 ug 50 ug 100 ug 200 ug 1 mg

Connective Tissue Growth Factor (CTGF) Antibody
20-abx103228	Abbexa	Ask for price Ask for price Ask for price Ask for price Ask for price	10 ug 50 ug 100 ug 200 ug 1 mg

Connective Tissue Growth Factor (CTGF) Antibody
20-abx103229	Abbexa	Ask for price Ask for price Ask for price Ask for price Ask for price	10 ug 50 ug 100 ug 200 ug 1 mg

Connective Tissue Growth Factor (CTGF) Antibody
20-abx103230	Abbexa	Ask for price Ask for price Ask for price Ask for price Ask for price	10 ug 50 ug 100 ug 200 ug 1 mg

Connective Tissue Growth Factor (CTGF) Antibody
20-abx103231	Abbexa	Ask for price Ask for price Ask for price Ask for price Ask for price	10 ug 50 ug 100 ug 200 ug 1 mg

Connective Tissue Growth Factor (CTGF) Antibody
abx027387-400ul	Abbexa	400 ul	EUR 627.6

Connective Tissue Growth Factor (Ctgf) Antibody
20-abx319302	Abbexa	Ask for price Ask for price Ask for price Ask for price Ask for price	20 ug 50 ug 100 ug 200 ug 1 mg

Connective tissue growth factor (CTGF) Antibody
20-abx214776	Abbexa	Ask for price Ask for price	50 ul 100 ul

Connective Tissue Growth Factor (CTGF) Antibody
20-abx225128	Abbexa	Ask for price Ask for price Ask for price	50 ul 100 ul 200 ul

Connective Tissue Growth Factor (CTGF) Antibody
abx232053-100ug	Abbexa	100 ug	EUR 610.8

Connective Tissue Growth Factor (CTGF) Antibody
abx232054-100ug	Abbexa	100 ug	EUR 610.8

Connective Tissue Growth Factor (CTGF) Antibody
abx037800-100ug	Abbexa	100 ug	EUR 469.2

Connective Tissue Growth Factor (CTGF) Antibody
abx433502-200ul	Abbexa	200 ul	EUR 543.6

Connective Tissue Growth Factor (CTGF) Antibody
abx033675-400ul	Abbexa	400 ul	EUR 627.6

Connective Tissue Growth Factor (CTGF) Antibody
abx033675-80l	Abbexa	80 µl	EUR 343.2

Connective Tissue Growth Factor (CTGF) Antibody
abx033676-400ul	Abbexa	400 ul	EUR 627.6

Connective Tissue Growth Factor (CTGF) Antibody
abx033676-80l	Abbexa	80 µl	EUR 343.2

Connective Tissue Growth Factor (CTGF) Antibody
abx025473-100ul	Abbexa	100 ul	EUR 627.6

CTGF (untagged)-Human connective tissue growth factor (CTGF)
SC118973	Origene Technologies GmbH	10 µg	Ask for price

Recombinant Connective Tissue Growth Factor (CTGF)
MBS2125400-001mg	MyBiosource	0.01mg	EUR 170

Recombinant Connective Tissue Growth Factor (CTGF)
MBS2125400-005mg	MyBiosource	0.05mg	EUR 300

Recombinant Connective Tissue Growth Factor (CTGF)
MBS2125400-01mg	MyBiosource	0.1mg	EUR 450

Recombinant Connective Tissue Growth Factor (CTGF)
MBS2125400-02mg	MyBiosource	0.2mg	EUR 550

Recombinant Connective Tissue Growth Factor (CTGF)
MBS2125400-05mg	MyBiosource	0.5mg	EUR 1050

Recombinant Connective Tissue Growth Factor (CTGF)
MBS2031787-001mg	MyBiosource	0.01mg	EUR 180

Recombinant Connective Tissue Growth Factor (CTGF)
MBS2031787-005mg	MyBiosource	0.05mg	EUR 325

Recombinant Connective Tissue Growth Factor (CTGF)
MBS2031787-01mg	MyBiosource	0.1mg	EUR 495

Recombinant Connective Tissue Growth Factor (CTGF)
MBS2031787-02mg	MyBiosource	0.2mg	EUR 610

Recombinant Connective Tissue Growth Factor (CTGF)
MBS2031787-05mg	MyBiosource	0.5mg	EUR 1160

Recombinant Connective Tissue Growth Factor (CTGF)
MBS2009064-001mg	MyBiosource	0.01mg	EUR 170

Recombinant Connective Tissue Growth Factor (CTGF)
MBS2009064-005mg	MyBiosource	0.05mg	EUR 295

Recombinant Connective Tissue Growth Factor (CTGF)
MBS2009064-01mg	MyBiosource	0.1mg	EUR 445

Recombinant Connective Tissue Growth Factor (CTGF)
MBS2009064-02mg	MyBiosource	0.2mg	EUR 540

Recombinant Connective Tissue Growth Factor (CTGF)
MBS2009064-05mg	MyBiosource	0.5mg	EUR 1025

Recombinant Connective Tissue Growth Factor (CTGF)
MBS2009146-001mg	MyBiosource	0.01mg	EUR 190

Recombinant Connective Tissue Growth Factor (CTGF)
MBS2009146-005mg	MyBiosource	0.05mg	EUR 345

Recombinant Connective Tissue Growth Factor (CTGF)
MBS2009146-01mg	MyBiosource	0.1mg	EUR 525

Recombinant Connective Tissue Growth Factor (CTGF)
MBS2009146-02mg	MyBiosource	0.2mg	EUR 640

Recombinant Connective Tissue Growth Factor (CTGF)
MBS2009146-05mg	MyBiosource	0.5mg	EUR 1230

Recombinant Connective Tissue Growth Factor (CTGF)
MBS2009579-001mg	MyBiosource	0.01mg	EUR 155

Recombinant Connective Tissue Growth Factor (CTGF)
MBS2009579-005mg	MyBiosource	0.05mg	EUR 260

Recombinant Connective Tissue Growth Factor (CTGF)
MBS2009579-01mg	MyBiosource	0.1mg	EUR 375

Mecasermin protein saved stability at 4°C for as much as 2 years. The quantitative and qualitative management indicated that Mecasermin is expressed appropriately (with out the preliminary methionine by Mass spectrometry), pure (with out endotoxin and different protein impurities), appropriate folding (FTIR, RF-HPLC), monomer type (SEC-HPLC) and lively (bioactivity check). Additionally, the purification outcomes revealed that expression in low temperature outcomes the environment friendly purification of the over-produced Mecasermin with a excessive amount and high quality.