Microbial transglutaminase from Streptomyces mobaraensis (MTG) has been extensively utilized in meals business and in addition in analysis and medical functions, since it may possibly site-specifically modify proteins by the cross-linking response of glutamine residue and the first amino group. The recombinant expression system of MTG in E. coli supplies higher accessibility for the researchers and thus can promote additional utilization of MTG. Herein, we report manufacturing of lively and soluble MTG in E. coli through the use of a chimeric protein of tobacco etch virus (TEV) protease and MTG zymogen.
A chimera of TEV protease and MTG zymogen with native propeptide resulted in lively MTG contaminated with cleaved propeptide because of the robust interplay between the propeptide and catalytic area of MTG. Introduction of mutations of K9R and Y11A to the propeptide facilitated dissociation of the cleaved propeptide from the catalytic area of MTG and lively MTG with none contamination of the propeptide was obtained. The particular exercise of the lively MTG was 22.7±2.6 U/mg. The profitable expression and purification of lively MTG through the use of the chimera protein of TEV protease and MTG zymogen with mutations within the propeptide can advance using MTG and the researches utilizing MTG mediated cross-linking reactions.
Technology of novel trimeric fragments of human SP-A and SP-D after recombinant soluble expression in E. coli
Surfactant therapy for neonatal respiratory misery syndrome has dramatically improved survival of preterm infants. Nonetheless, this has resulted in a markedly elevated incidence of sequelae corresponding to neonatal persistent inflammatory lung illness. The present surfactant preparations in medical use lack the pure lung defence proteins surfactant proteins (SP)-A and D. These are identified to have anti-inflammatory and anti-infective properties important for sustaining wholesome non-inflamed lungs.
Supplementation of at the moment accessible animal derived surfactant therapeutics with these anti-inflammatory proteins within the first few days of life may stop the event of inflammatory lung illness in untimely infants. Nonetheless, present methods for manufacturing of recombinant variations of SP-A and SP-D require a posh solubilisation and refolding protocol limiting expression at scale for drug improvement. Utilizing a novel solubility tag, we describe the expression and purification of recombinant fragments of human (rfh) SP-A and SP-D utilizing Escherichia coli with out the necessity for refolding.
We obtained a imply (± SD) of 23.3 (± 5.4) mg and 86 mg (± 3.5) per litre yield of rfhSP-A and rfhSP-D, respectively. rfhSP-D was trimeric and 68% sure to a ManNAc-affinity column, giving a closing yield of 57.5 mg/litre of extremely pure protein, considerably increased than the three.Three mg/litre obtained via the usual refolding protocol. Additional optimisation of this novel lab primarily based methodology may doubtlessly make rfhSP-A and rfhSP-D manufacturing extra commercially possible to allow improvement of novel therapeutics for the therapy of lung an infection and irritation.

Expression, solubilization, refolding and closing purification of recombinant proteins as expressed within the type of “classical inclusion our bodies” in E. coli
Escherichia coli has been most generally used for manufacturing of the recombinant proteins. Over-expression of the recombinant proteins is the mainspring of the inclusion our bodies formation. The refolding of those proteins into bioactive types is cumbersome and partly time-consuming. Within the current examine, we reviewed and mentioned most points concerning the restoration of "classical inclusion our bodies" by specializing in our earlier experiences.
Performing correct strategies of expression, solubilization, refolding and closing purification of those proteins, would make it attainable to get well increased quantities of pro-teins into the native type with applicable conformation. Usually, offering gentle circumstances and correct refolding buffers, would result in get well greater than 40% of inclusion our bodies into bioactive and native conformation.
Efficient Course of Growth for Excessive-level Manufacturing, Purification, Formulation, and Characterization of Recombinant Mecasermin in E. coli
Over-production recombinant Mecasermin was achieved by investigation impact of three components, temperature, inducer quantity and tradition media in three ranges in accordance with Taguchi statistical design in E. coli in a bench-scale bioreactor. In optimum circumstances (induction temperature 28°C, TB+G medium, with 0.1 mM IPTG as inducer) 0.84 g/L Mecasermin with expression ranges of 38% of whole protein and 4.13 g/L closing dry cell biomass was produced, that is likely one of the highest values recombinant protein has been reported within the batch system.
The cell disruption was executed by lysozyme pretreatment with sonication to the environment friendly purification of Mecasermin. The remoted and washed inclusion our bodies had been solubilized in Gdn-HCl at pH 5.Four and folded with glutathione and purified with gel filtration. The purified rhIGF-1 (Mecasermin) was formulated with arginine.
Porcine Connective Tissue Growth Factor (CTGF) ELISA Kit |
DLR-CTGF-p-48T |
DL Develop |
48T |
EUR 591.6 |
|
Description: A sandwich quantitative ELISA assay kit for detection of Porcine Connective Tissue Growth Factor (CTGF) in samples from serum, plasma, tissue homogenates or other biological fluids. |
Porcine Connective Tissue Growth Factor (CTGF) ELISA Kit |
DLR-CTGF-p-96T |
DL Develop |
96T |
EUR 769.2 |
|
Description: A sandwich quantitative ELISA assay kit for detection of Porcine Connective Tissue Growth Factor (CTGF) in samples from serum, plasma, tissue homogenates or other biological fluids. |
Rat Connective Tissue Growth Factor (CTGF) ELISA Kit |
DLR-CTGF-Ra-48T |
DL Develop |
48T |
EUR 544.8 |
|
Description: A sandwich quantitative ELISA assay kit for detection of Rat Connective Tissue Growth Factor (CTGF) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
Rat Connective Tissue Growth Factor (CTGF) ELISA Kit |
DLR-CTGF-Ra-96T |
DL Develop |
96T |
EUR 704.4 |
|
Description: A sandwich quantitative ELISA assay kit for detection of Rat Connective Tissue Growth Factor (CTGF) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
Rabbit Connective Tissue Growth Factor (CTGF) ELISA Kit |
DLR-CTGF-Rb-48T |
DL Develop |
48T |
EUR 544.8 |
|
Description: A sandwich quantitative ELISA assay kit for detection of Rabbit Connective Tissue Growth Factor (CTGF) in samples from serum, plasma or other biological fluids. |
Rabbit Connective Tissue Growth Factor (CTGF) ELISA Kit |
DLR-CTGF-Rb-96T |
DL Develop |
96T |
EUR 704.4 |
|
Description: A sandwich quantitative ELISA assay kit for detection of Rabbit Connective Tissue Growth Factor (CTGF) in samples from serum, plasma or other biological fluids. |
Bovine Connective Tissue Growth Factor (CTGF) ELISA Kit |
RD-CTGF-b-48Tests |
Reddot Biotech |
48 Tests |
EUR 592.8 |
Bovine Connective Tissue Growth Factor (CTGF) ELISA Kit |
RD-CTGF-b-96Tests |
Reddot Biotech |
96 Tests |
EUR 820.8 |
Human Connective Tissue Growth Factor (CTGF) ELISA Kit |
RD-CTGF-Hu-48Tests |
Reddot Biotech |
48 Tests |
EUR 450 |
Human Connective Tissue Growth Factor (CTGF) ELISA Kit |
RD-CTGF-Hu-96Tests |
Reddot Biotech |
96 Tests |
EUR 618 |
Mouse Connective Tissue Growth Factor (CTGF) ELISA Kit |
RD-CTGF-Mu-48Tests |
Reddot Biotech |
48 Tests |
EUR 514.8 |
Mouse Connective Tissue Growth Factor (CTGF) ELISA Kit |
RD-CTGF-Mu-96Tests |
Reddot Biotech |
96 Tests |
EUR 709.2 |
Porcine Connective Tissue Growth Factor (CTGF) ELISA Kit |
RD-CTGF-p-48Tests |
Reddot Biotech |
48 Tests |
EUR 592.8 |
Porcine Connective Tissue Growth Factor (CTGF) ELISA Kit |
RD-CTGF-p-96Tests |
Reddot Biotech |
96 Tests |
EUR 820.8 |
Rat Connective Tissue Growth Factor (CTGF) ELISA Kit |
RD-CTGF-Ra-48Tests |
Reddot Biotech |
48 Tests |
EUR 540 |
Rat Connective Tissue Growth Factor (CTGF) ELISA Kit |
RD-CTGF-Ra-96Tests |
Reddot Biotech |
96 Tests |
EUR 746.4 |
Rabbit Connective Tissue Growth Factor (CTGF) ELISA Kit |
RD-CTGF-Rb-48Tests |
Reddot Biotech |
48 Tests |
EUR 540 |
Rabbit Connective Tissue Growth Factor (CTGF) ELISA Kit |
RD-CTGF-Rb-96Tests |
Reddot Biotech |
96 Tests |
EUR 746.4 |
Bovine Connective Tissue Growth Factor (CTGF) ELISA Kit |
RDR-CTGF-b-48Tests |
Reddot Biotech |
48 Tests |
EUR 619.2 |
Bovine Connective Tissue Growth Factor (CTGF) ELISA Kit |
RDR-CTGF-b-96Tests |
Reddot Biotech |
96 Tests |
EUR 859.2 |
Chicken Connective Tissue Growth Factor (CTGF) ELISA Kit |
RDR-CTGF-Ch-48Tests |
Reddot Biotech |
48 Tests |
EUR 619.2 |
Chicken Connective Tissue Growth Factor (CTGF) ELISA Kit |
RDR-CTGF-Ch-96Tests |
Reddot Biotech |
96 Tests |
EUR 859.2 |
Human Connective Tissue Growth Factor (CTGF) ELISA Kit |
RDR-CTGF-Hu-48Tests |
Reddot Biotech |
48 Tests |
EUR 469.2 |
Human Connective Tissue Growth Factor (CTGF) ELISA Kit |
RDR-CTGF-Hu-96Tests |
Reddot Biotech |
96 Tests |
EUR 645.6 |
Mouse Connective Tissue Growth Factor (CTGF) ELISA Kit |
RDR-CTGF-Mu-48Tests |
Reddot Biotech |
48 Tests |
EUR 536.4 |
Mouse Connective Tissue Growth Factor (CTGF) ELISA Kit |
RDR-CTGF-Mu-96Tests |
Reddot Biotech |
96 Tests |
EUR 741.6 |
Porcine Connective Tissue Growth Factor (CTGF) ELISA Kit |
RDR-CTGF-p-48Tests |
Reddot Biotech |
48 Tests |
EUR 619.2 |
Porcine Connective Tissue Growth Factor (CTGF) ELISA Kit |
RDR-CTGF-p-96Tests |
Reddot Biotech |
96 Tests |
EUR 859.2 |
Rat Connective Tissue Growth Factor (CTGF) ELISA Kit |
RDR-CTGF-Ra-48Tests |
Reddot Biotech |
48 Tests |
EUR 564 |
Rat Connective Tissue Growth Factor (CTGF) ELISA Kit |
RDR-CTGF-Ra-96Tests |
Reddot Biotech |
96 Tests |
EUR 781.2 |
Rabbit Connective Tissue Growth Factor (CTGF) ELISA Kit |
RDR-CTGF-Rb-48Tests |
Reddot Biotech |
48 Tests |
EUR 564 |
Rabbit Connective Tissue Growth Factor (CTGF) ELISA Kit |
RDR-CTGF-Rb-96Tests |
Reddot Biotech |
96 Tests |
EUR 781.2 |
Pig Connective Tissue Growth Factor (CTGF) Protein |
20-abx066093 |
Abbexa |
-
EUR 878.40
-
EUR 343.20
-
EUR 2766.00
-
EUR 1062.00
-
EUR 627.60
|
- 100 ug
- 10 ug
- 1 mg
- 200 ug
- 50 ug
|
|
Connective Tissue Growth Factor (CTGF) Antibody |
20-abx171909 |
Abbexa |
|
|
|
Connective Tissue Growth Factor (CTGF) Antibody |
20-abx171910 |
Abbexa |
|
|
|
Connective Tissue Growth Factor (CTGF) Antibody |
20-abx132193 |
Abbexa |
-
EUR 510.00
-
EUR 159.60
-
EUR 1412.40
-
EUR 693.60
-
EUR 393.60
|
- 100 ug
- 10 ug
- 1 mg
- 200 ug
- 50 ug
|
|
Connective Tissue Growth Factor (CTGF) Antibody |
20-abx132350 |
Abbexa |
-
EUR 393.60
-
EUR 978.00
-
EUR 510.00
-
EUR 184.80
-
EUR 309.60
|
- 100 ug
- 1 mg
- 200 ug
- 20 ug
- 50 ug
|
|
Connective Tissue Growth Factor (CTGF) Antibody |
20-abx132360 |
Abbexa |
-
EUR 393.60
-
EUR 994.80
-
EUR 510.00
-
EUR 184.80
-
EUR 309.60
|
- 100 ug
- 1 mg
- 200 ug
- 20 ug
- 50 ug
|
|
Connective Tissue Growth Factor (CTGF) Antibody |
20-abx104836 |
Abbexa |
-
EUR 543.60
-
EUR 159.60
-
EUR 1545.60
-
EUR 744.00
-
EUR 410.40
|
- 100 ug
- 10 ug
- 1 mg
- 200 ug
- 50 ug
|
|
Connective Tissue Growth Factor (CTGF) Antibody |
20-abx103225 |
Abbexa |
-
EUR 427.20
-
EUR 159.60
-
EUR 1195.20
-
EUR 594.00
-
EUR 360.00
|
- 100 ug
- 10 ug
- 1 mg
- 200 ug
- 50 ug
|
|
Connective Tissue Growth Factor (CTGF) Antibody |
20-abx103226 |
Abbexa |
-
EUR 427.20
-
EUR 159.60
-
EUR 1195.20
-
EUR 594.00
-
EUR 360.00
|
- 100 ug
- 10 ug
- 1 mg
- 200 ug
- 50 ug
|
|
Connective Tissue Growth Factor (CTGF) Antibody |
20-abx103227 |
Abbexa |
-
EUR 360.00
-
EUR 861.60
-
EUR 460.80
-
EUR 184.80
-
EUR 292.80
|
- 100 ug
- 1 mg
- 200 ug
- 20 ug
- 50 ug
|
|
Connective Tissue Growth Factor (CTGF) Antibody |
20-abx103228 |
Abbexa |
-
EUR 493.20
-
EUR 159.60
-
EUR 1362.00
-
EUR 661.20
-
EUR 376.80
|
- 100 ug
- 10 ug
- 1 mg
- 200 ug
- 50 ug
|
|
Connective Tissue Growth Factor (CTGF) Antibody |
20-abx103229 |
Abbexa |
-
EUR 510.00
-
EUR 159.60
-
EUR 1412.40
-
EUR 693.60
-
EUR 393.60
|
- 100 ug
- 10 ug
- 1 mg
- 200 ug
- 50 ug
|
|
Connective Tissue Growth Factor (CTGF) Antibody |
20-abx103230 |
Abbexa |
-
EUR 543.60
-
EUR 159.60
-
EUR 1545.60
-
EUR 744.00
-
EUR 410.40
|
- 100 ug
- 10 ug
- 1 mg
- 200 ug
- 50 ug
|
|
Connective Tissue Growth Factor (CTGF) Antibody |
20-abx103231 |
Abbexa |
-
EUR 560.40
-
EUR 159.60
-
EUR 1646.40
-
EUR 777.60
-
EUR 427.20
|
- 100 ug
- 10 ug
- 1 mg
- 200 ug
- 50 ug
|
|
Connective Tissue Growth Factor (CTGF) Antibody |
abx037800-100ug |
Abbexa |
100 ug |
EUR 469.2 |
|
Connective Tissue Growth Factor (CTGF) Antibody |
20-abx123700 |
Abbexa |
|
|
|
Connective Tissue Growth Factor (CTGF) Antibody |
20-abx109670 |
Abbexa |
-
EUR 493.20
-
EUR 2214.00
-
EUR 718.80
-
EUR 218.40
-
EUR 360.00
|
- 100 ug
- 1 mg
- 200 ug
- 20 ug
- 50 ug
|
|
Connective Tissue Growth Factor (CTGF) Antibody |
20-abx131768 |
Abbexa |
-
EUR 393.60
-
EUR 1011.60
-
EUR 526.80
-
EUR 184.80
-
EUR 309.60
|
- 100 ug
- 1 mg
- 200 ug
- 20 ug
- 50 ug
|
|
Connective Tissue Growth Factor (CTGF) Antibody |
20-abx125726 |
Abbexa |
-
EUR 493.20
-
EUR 710.40
-
EUR 218.40
-
EUR 376.80
|
- 100 ul
- 200 ul
- 20 ul
- 50 ul
|
|
Connective Tissue Growth Factor (CTGF) Antibody |
20-abx125727 |
Abbexa |
-
EUR 493.20
-
EUR 710.40
-
EUR 218.40
-
EUR 376.80
|
- 100 ul
- 200 ul
- 20 ul
- 50 ul
|
|
Connective Tissue Growth Factor (CTGF) Antibody |
20-abx125728 |
Abbexa |
-
EUR 493.20
-
EUR 710.40
-
EUR 376.80
|
|
|
Connective Tissue Growth Factor (CTGF) Antibody |
abx033675-400ul |
Abbexa |
400 ul |
EUR 627.6 |
|
Connective Tissue Growth Factor (CTGF) Antibody |
abx033675-80l |
Abbexa |
80 µl |
EUR 343.2 |
|
Connective Tissue Growth Factor (CTGF) Antibody |
abx033676-400ul |
Abbexa |
400 ul |
EUR 627.6 |
|
Connective Tissue Growth Factor (CTGF) Antibody |
abx033676-80l |
Abbexa |
80 µl |
EUR 343.2 |
|
Connective Tissue Growth Factor (CTGF) Antibody |
abx027387-400ul |
Abbexa |
400 ul |
EUR 627.6 |
|
Connective Tissue Growth Factor (CTGF) Antibody |
abx027387-80l |
Abbexa |
80 µl |
EUR 343.2 |
|
Connective Tissue Growth Factor (CTGF) Antibody |
abx025473-100ul |
Abbexa |
100 ul |
EUR 627.6 |
|
Connective Tissue Growth Factor (CTGF) Antibody |
20-abx225128 |
Abbexa |
-
EUR 444.00
-
EUR 727.20
-
EUR 376.80
|
|
|
Connective Tissue Growth Factor (Ctgf) Antibody |
20-abx319302 |
Abbexa |
-
EUR 493.20
-
EUR 2214.00
-
EUR 718.80
-
EUR 218.40
-
EUR 360.00
|
- 100 ug
- 1 mg
- 200 ug
- 20 ug
- 50 ug
|
|
Connective Tissue Growth Factor (CTGF) Antibody |
abx433502-200ul |
Abbexa |
200 ul |
EUR 543.6 |
|
Connective Tissue Growth Factor (CTGF) Antibody |
abx232053-100ug |
Abbexa |
100 ug |
EUR 610.8 |
|
Connective Tissue Growth Factor (CTGF) Antibody |
abx232054-100ug |
Abbexa |
100 ug |
EUR 610.8 |
|
Connective Tissue Growth Factor (CTGF) Antibody |
20-abx175995 |
Abbexa |
|
|
|
Connective Tissue Growth Factor (CTGF) Antibody |
20-abx175996 |
Abbexa |
|
|
|
Connective Tissue Growth Factor (CTGF) Antibody |
20-abx175997 |
Abbexa |
|
|
|
Connective tissue growth factor (CTGF) Antibody |
20-abx214776 |
Abbexa |
|
|
|
Active Connective Tissue Growth Factor (CTGF) |
4-APA010Ra01 |
Cloud-Clone |
-
EUR 797.38
-
EUR 337.20
-
EUR 2660.16
-
EUR 966.72
-
EUR 1813.44
-
EUR 609.60
-
EUR 6470.40
|
- 100 ug
- 10ug
- 1 mg
- 200 ug
- 500 ug
- 50ug
- 5 mg
|
|
Description: Recombinant Rat Connective Tissue Growth Factor expressed in: Available from E.coli, Yeast, Baculovirus and Mammalian cells |
Human Connective tissue growth factor (CTGF) |
1-CSB-EP006147HU |
Cusabio |
-
EUR 606.00
-
EUR 318.00
-
EUR 2192.40
-
EUR 919.20
-
EUR 1461.60
-
EUR 402.00
|
- 100ug
- 10ug
- 1MG
- 200ug
- 500ug
- 50ug
|
|
Description: Recombinant Human Connective tissue growth factor(CTGF) expressed in E.coli |
Human Connective tissue growth factor (CTGF) |
1-CSB-EP006147HU1 |
Cusabio |
-
EUR 456.00
-
EUR 256.80
-
EUR 1570.80
-
EUR 672.00
-
EUR 1047.60
-
EUR 314.40
|
- 100ug
- 10ug
- 1MG
- 200ug
- 500ug
- 50ug
|
|
Description: Recombinant Human Connective tissue growth factor(CTGF),partial expressed in E.coli |
Mouse Connective tissue growth factor (Ctgf) |
1-CSB-EP006147MO |
Cusabio |
-
EUR 606.00
-
EUR 318.00
-
EUR 2192.40
-
EUR 919.20
-
EUR 1461.60
-
EUR 402.00
|
- 100ug
- 10ug
- 1MG
- 200ug
- 500ug
- 50ug
|
|
Description: Recombinant Mouse Connective tissue growth factor (Ctgf),partial expressed in E.coli |
Mouse Connective tissue growth factor (Ctgf) |
1-CSB-EP006147MOe0 |
Cusabio |
-
EUR 606.00
-
EUR 318.00
-
EUR 2192.40
-
EUR 919.20
-
EUR 1461.60
-
EUR 402.00
|
- 100ug
- 10ug
- 1MG
- 200ug
- 500ug
- 50ug
|
|
Description: Recombinant Mouse Connective tissue growth factor(Ctgf),partial expressed in E.coli |
Recombinant Connective Tissue Growth Factor (CTGF) |
4-RPA010Bo01 |
Cloud-Clone |
-
EUR 636.10
-
EUR 294.00
-
EUR 2055.36
-
EUR 765.12
-
EUR 1410.24
-
EUR 501.60
-
EUR 4958.40
|
- 100 ug
- 10ug
- 1 mg
- 200 ug
- 500 ug
- 50ug
- 5 mg
|
|
Description: Recombinant Bovine Connective Tissue Growth Factor expressed in: E.coli |
Recombinant Connective Tissue Growth Factor (CTGF) |
4-RPA010Hu01 |
Cloud-Clone |
-
EUR 571.58
-
EUR 276.00
-
EUR 1813.44
-
EUR 684.48
-
EUR 1248.96
-
EUR 458.40
-
EUR 4353.60
|
- 100 ug
- 10ug
- 1 mg
- 200 ug
- 500 ug
- 50ug
- 5 mg
|
|
Description: Recombinant Human Connective Tissue Growth Factor expressed in: E.coli |
Recombinant Connective Tissue Growth Factor (CTGF) |
4-RPA010Hu02 |
Cloud-Clone |
-
EUR 571.58
-
EUR 276.00
-
EUR 1813.44
-
EUR 684.48
-
EUR 1248.96
-
EUR 458.40
-
EUR 4353.60
|
- 100 ug
- 10ug
- 1 mg
- 200 ug
- 500 ug
- 50ug
- 5 mg
|
|
Description: Recombinant Human Connective Tissue Growth Factor expressed in: E.coli |
Recombinant Connective Tissue Growth Factor (CTGF) |
4-RPA010Mu01 |
Cloud-Clone |
-
EUR 603.84
-
EUR 285.60
-
EUR 1934.40
-
EUR 724.80
-
EUR 1329.60
-
EUR 480.00
-
EUR 4656.00
|
- 100 ug
- 10ug
- 1 mg
- 200 ug
- 500 ug
- 50ug
- 5 mg
|
|
Description: Recombinant Mouse Connective Tissue Growth Factor expressed in: E.coli |
Recombinant Connective Tissue Growth Factor (CTGF) |
4-RPA010Ov01 |
Cloud-Clone |
-
EUR 668.35
-
EUR 302.40
-
EUR 2176.32
-
EUR 805.44
-
EUR 1490.88
-
EUR 523.20
-
EUR 5260.80
|
- 100 ug
- 10ug
- 1 mg
- 200 ug
- 500 ug
- 50ug
- 5 mg
|
|
Description: Recombinant Sheep Connective Tissue Growth Factor expressed in: E.coli |
Recombinant Connective Tissue Growth Factor (CTGF) |
4-RPA010Po01 |
Cloud-Clone |
-
EUR 636.10
-
EUR 294.00
-
EUR 2055.36
-
EUR 765.12
-
EUR 1410.24
-
EUR 501.60
-
EUR 4958.40
|
- 100 ug
- 10ug
- 1 mg
- 200 ug
- 500 ug
- 50ug
- 5 mg
|
|
Description: Recombinant Pig Connective Tissue Growth Factor expressed in: E.coli |
Recombinant Connective Tissue Growth Factor (CTGF) |
4-RPA010Ra01 |
Cloud-Clone |
-
EUR 496.32
-
EUR 256.80
-
EUR 1531.20
-
EUR 590.40
-
EUR 1060.80
-
EUR 408.00
-
EUR 3648.00
|
- 100 ug
- 10ug
- 1 mg
- 200 ug
- 500 ug
- 50ug
- 5 mg
|
|
Description: Recombinant Rat Connective Tissue Growth Factor expressed in: E.coli |
Pig Connective Tissue Growth Factor (CTGF) ELISA Kit |
20-abx154906 |
Abbexa |
-
EUR 9174.00
-
EUR 4888.80
-
EUR 1130.40
|
- 10 × 96 tests
- 5 × 96 tests
- 96 tests
|
|
Pig Connective Tissue Growth Factor (CTGF) ELISA Kit |
abx573322-96tests |
Abbexa |
96 tests |
EUR 990 |
|
Pig Connective Tissue Growth Factor (CTGF) CLIA Kit |
20-abx190822 |
Abbexa |
-
EUR 11090.40
-
EUR 5904.00
-
EUR 1356.00
|
- 10 × 96 tests
- 5 × 96 tests
- 96 tests
|
|
Connective Tissue Growth Factor (CTGF) Polyclonal Antibody (Pig) |
4-PAA010Po01 |
Cloud-Clone |
-
EUR 310.80
-
EUR 3232.80
-
EUR 800.40
-
EUR 391.20
-
EUR 261.60
|
- 100ul
- 10ml
- 1ml
- 200ul
- 20ul
|
|
Description: A Rabbit polyclonal antibody against Pig Connective Tissue Growth Factor (CTGF) |
Pig Connective Tissue Growth Factor (CTGF) ELISA Kit |
SEA010Po-10x96wellstestplate |
Cloud-Clone |
10x96-wells test plate |
EUR 6117.62 |
|
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Pig Connective Tissue Growth Factor (CTGF) in serum, plasma, tissue homogenates and other biological fluids. |
Pig Connective Tissue Growth Factor (CTGF) ELISA Kit |
SEA010Po-1x48wellstestplate |
Cloud-Clone |
1x48-wells test plate |
EUR 608.98 |
|
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Pig Connective Tissue Growth Factor (CTGF) in serum, plasma, tissue homogenates and other biological fluids. |
Pig Connective Tissue Growth Factor (CTGF) ELISA Kit |
SEA010Po-1x96wellstestplate |
Cloud-Clone |
1x96-wells test plate |
EUR 818.54 |
|
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Pig Connective Tissue Growth Factor (CTGF) in serum, plasma, tissue homogenates and other biological fluids. |
Pig Connective Tissue Growth Factor (CTGF) ELISA Kit |
SEA010Po-5x96wellstestplate |
Cloud-Clone |
5x96-wells test plate |
EUR 3323.45 |
|
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Pig Connective Tissue Growth Factor (CTGF) in serum, plasma, tissue homogenates and other biological fluids. |
Pig Connective Tissue Growth Factor (CTGF) ELISA Kit |
4-SEA010Po |
Cloud-Clone |
-
EUR 6178.80
-
EUR 3264.00
-
EUR 819.60
|
- 10 plates of 96 wells
- 5 plates of 96 wells
- 1 plate of 96 wells
|
|
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Pig Connective Tissue Growth Factor (CTGF) in samples from Serum, plasma, tissue homogenates and other biological fluids. with no significant corss-reactivity with analogues from other species. |
Pig Connective Tissue Growth Factor (CTGF)CLIA Kit |
SCA010Po-10x96wellstestplate |
Cloud-Clone |
10x96-wells test plate |
EUR 8096.83 |
|
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Pig Connective Tissue Growth Factor (CTGF) in serum, plasma and other biological fluids. |
Pig Connective Tissue Growth Factor (CTGF)CLIA Kit |
SCA010Po-1x48wellstestplate |
Cloud-Clone |
1x48-wells test plate |
EUR 771.97 |
|
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Pig Connective Tissue Growth Factor (CTGF) in serum, plasma and other biological fluids. |
Pig Connective Tissue Growth Factor (CTGF)CLIA Kit |
SCA010Po-1x96wellstestplate |
Cloud-Clone |
1x96-wells test plate |
EUR 1051.39 |
|
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Pig Connective Tissue Growth Factor (CTGF) in serum, plasma and other biological fluids. |
Pig Connective Tissue Growth Factor (CTGF)CLIA Kit |
SCA010Po-5x96wellstestplate |
Cloud-Clone |
5x96-wells test plate |
EUR 4371.26 |
|
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Pig Connective Tissue Growth Factor (CTGF) in serum, plasma and other biological fluids. |
Pig Connective Tissue Growth Factor (CTGF) CLIA Kit |
4-SCA010Po |
Cloud-Clone |
-
EUR 8157.60
-
EUR 4371.60
-
EUR 1052.40
|
- 10 plates of 96 wells
- 5 plates of 96 wells
- 1 plate of 96 wells
|
|
Description: Double-antibody Sandwich chemiluminescent immunoassay for detection of Pig Connective Tissue Growth Factor (CTGF)Serum, plasma and other biological fluids |
CLIA kit for Human CTGF (Connective Tissue Growth Factor) |
E-CL-H0579 |
Elabscience Biotech |
1 plate of 96 wells |
EUR 700.8 |
|
Description: A sandwich CLIA kit for quantitative measurement of Human CTGF (Connective Tissue Growth Factor) in samples from Serum, Plasma, Cell supernatant |
CLIA kit for Mouse CTGF (Connective Tissue Growth Factor) |
E-CL-M0220 |
Elabscience Biotech |
1 plate of 96 wells |
EUR 700.8 |
|
Description: A sandwich CLIA kit for quantitative measurement of Mouse CTGF (Connective Tissue Growth Factor) in samples from Serum, Plasma, Cell supernatant |
CLIA kit for Rat CTGF (Connective Tissue Growth Factor) |
E-CL-R0173 |
Elabscience Biotech |
1 plate of 96 wells |
EUR 700.8 |
|
Description: A sandwich CLIA kit for quantitative measurement of Rat CTGF (Connective Tissue Growth Factor) in samples from Serum, Plasma, Cell supernatant |
ELISA kit for Human CTGF (Connective Tissue Growth Factor) |
E-EL-H0828 |
Elabscience Biotech |
1 plate of 96 wells |
EUR 640.8 |
|
Description: A sandwich ELISA kit for quantitative measurement of Human CTGF (Connective Tissue Growth Factor) in samples from Serum, Plasma, Cell supernatant |
ELISA kit for Mouse CTGF (Connective Tissue Growth Factor) |
E-EL-M0340 |
Elabscience Biotech |
1 plate of 96 wells |
EUR 640.8 |
|
Description: A sandwich ELISA kit for quantitative measurement of Mouse CTGF (Connective Tissue Growth Factor) in samples from Serum, Plasma, Cell supernatant |
ELISA kit for Rat CTGF (Connective Tissue Growth Factor) |
E-EL-R0259 |
Elabscience Biotech |
1 plate of 96 wells |
EUR 640.8 |
|
Description: A sandwich ELISA kit for quantitative measurement of Rat CTGF (Connective Tissue Growth Factor) in samples from Serum, Plasma, Cell supernatant |
Guinea pig Connective Tissue Growth Factor (CTGF) ELISA Kit |
abx358103-96tests |
Abbexa |
96 tests |
EUR 990 |
|
ELISA kit for Pig CTGF (Connective Tissue Growth Factor) |
ELK5960 |
ELK Biotech |
1 plate of 96 wells |
EUR 631.2 |
|
Description: A sandwich ELISA kit for detection of Connective Tissue Growth Factor from Pig in samples from blood, serum, plasma, cell culture fluid and other biological fluids. |
Connective Tissue Growth Factor (CTGF) Polyclonal Antibody (Pig), APC |
4-PAA010Po01-APC |
Cloud-Clone |
-
EUR 435.60
-
EUR 4232.40
-
EUR 1170.00
-
EUR 558.00
-
EUR 271.20
|
- 100ul
- 10ml
- 1ml
- 200ul
- 20ul
|
|
Description: A Rabbit polyclonal antibody against Pig Connective Tissue Growth Factor (CTGF). This antibody is labeled with APC. |
Connective Tissue Growth Factor (CTGF) Polyclonal Antibody (Pig), Biotinylated |
4-PAA010Po01-Biotin |
Cloud-Clone |
-
EUR 388.80
-
EUR 3172.80
-
EUR 927.60
-
EUR 478.80
-
EUR 268.80
|
- 100ul
- 10ml
- 1ml
- 200ul
- 20ul
|
|
Description: A Rabbit polyclonal antibody against Pig Connective Tissue Growth Factor (CTGF). This antibody is labeled with Biotin. |
Connective Tissue Growth Factor (CTGF) Polyclonal Antibody (Pig), Cy3 |
4-PAA010Po01-Cy3 |
Cloud-Clone |
-
EUR 531.60
-
EUR 5593.20
-
EUR 1510.80
-
EUR 693.60
-
EUR 312.00
|
- 100ul
- 10ml
- 1ml
- 200ul
- 20ul
|
|
Description: A Rabbit polyclonal antibody against Pig Connective Tissue Growth Factor (CTGF). This antibody is labeled with Cy3. |
Connective Tissue Growth Factor (CTGF) Polyclonal Antibody (Pig), FITC |
4-PAA010Po01-FITC |
Cloud-Clone |
-
EUR 372.00
-
EUR 3409.20
-
EUR 960.00
-
EUR 470.40
-
EUR 241.20
|
- 100ul
- 10ml
- 1ml
- 200ul
- 20ul
|
|
Description: A Rabbit polyclonal antibody against Pig Connective Tissue Growth Factor (CTGF). This antibody is labeled with FITC. |
Connective Tissue Growth Factor (CTGF) Polyclonal Antibody (Pig), HRP |
4-PAA010Po01-HRP |
Cloud-Clone |
-
EUR 397.20
-
EUR 3687.60
-
EUR 1034.40
-
EUR 502.80
-
EUR 255.60
|
- 100ul
- 10ml
- 1ml
- 200ul
- 20ul
|
|
Description: A Rabbit polyclonal antibody against Pig Connective Tissue Growth Factor (CTGF). This antibody is labeled with HRP. |
Connective Tissue Growth Factor (CTGF) Polyclonal Antibody (Pig), PE |
4-PAA010Po01-PE |
Cloud-Clone |
-
EUR 372.00
-
EUR 3409.20
-
EUR 960.00
-
EUR 470.40
-
EUR 241.20
|
- 100ul
- 10ml
- 1ml
- 200ul
- 20ul
|
|
Description: A Rabbit polyclonal antibody against Pig Connective Tissue Growth Factor (CTGF). This antibody is labeled with PE. |
Human Connective Tissue Growth Factor (CTGF) Antibody |
35148-05111 |
AssayPro |
150 ug |
EUR 313.2 |
Cow Connective Tissue Growth Factor (CTGF) Protein |
20-abx168009 |
Abbexa |
-
EUR 878.40
-
EUR 343.20
-
EUR 2766.00
-
EUR 1062.00
-
EUR 627.60
|
- 100 ug
- 10 ug
- 1 mg
- 200 ug
- 50 ug
|
|
Human Connective Tissue Growth Factor (CTGF) Protein |
20-abx066091 |
Abbexa |
-
EUR 794.40
-
EUR 326.40
-
EUR 2448.00
-
EUR 944.40
-
EUR 577.20
|
- 100 ug
- 10 ug
- 1 mg
- 200 ug
- 50 ug
|
|
Mouse Connective Tissue Growth Factor (CTGF) Protein |
20-abx066092 |
Abbexa |
-
EUR 844.80
-
EUR 343.20
-
EUR 2598.00
-
EUR 994.80
-
EUR 594.00
|
- 100 ug
- 10 ug
- 1 mg
- 200 ug
- 50 ug
|
|
Rat Connective Tissue Growth Factor (CTGF) Protein |
20-abx066094 |
Abbexa |
-
EUR 693.60
-
EUR 309.60
-
EUR 2064.00
-
EUR 828.00
-
EUR 510.00
|
- 100 ug
- 10 ug
- 1 mg
- 200 ug
- 50 ug
|
|
Human Connective Tissue Growth Factor (CTGF) Protein |
20-abx066095 |
Abbexa |
-
EUR 794.40
-
EUR 326.40
-
EUR 2448.00
-
EUR 944.40
-
EUR 577.20
|
- 100 ug
- 10 ug
- 1 mg
- 200 ug
- 50 ug
|
|
Sheep Connective Tissue Growth Factor (CTGF) Protein |
20-abx066096 |
Abbexa |
-
EUR 927.60
-
EUR 360.00
-
EUR 2932.80
-
EUR 1111.20
-
EUR 661.20
|
- 100 ug
- 10 ug
- 1 mg
- 200 ug
- 50 ug
|
|
Connective tissue growth factor (CTGF) Antibody (Biotin) |
20-abx105285 |
Abbexa |
-
EUR 493.20
-
EUR 2214.00
-
EUR 718.80
-
EUR 218.40
-
EUR 360.00
|
- 100 ug
- 1 mg
- 200 ug
- 20 ug
- 50 ug
|
|
Connective tissue growth factor (CTGF) Antibody (HRP) |
20-abx108121 |
Abbexa |
-
EUR 493.20
-
EUR 2214.00
-
EUR 718.80
-
EUR 218.40
-
EUR 360.00
|
- 100 ug
- 1 mg
- 200 ug
- 20 ug
- 50 ug
|
|
Connective tissue growth factor (CTGF) Antibody (FITC) |
20-abx106704 |
Abbexa |
-
EUR 493.20
-
EUR 2214.00
-
EUR 718.80
-
EUR 218.40
-
EUR 360.00
|
- 100 ug
- 1 mg
- 200 ug
- 20 ug
- 50 ug
|
|
Connective Tissue Growth Factor (CTGF) Antibody Pair |
abx117594-1pair5x96wellplates |
Abbexa |
1 pair (5x96 well plates) |
EUR 1212 |
|
Connective Tissue Growth Factor (CTGF) Antibody (FITC) |
20-abx271026 |
Abbexa |
-
EUR 376.80
-
EUR 243.60
-
EUR 944.40
-
EUR 493.20
-
EUR 326.40
|
- 100 ug
- 10 ug
- 1 mg
- 200 ug
- 50 ug
|
|
Connective Tissue Growth Factor (CTGF) Antibody (Biotin) |
20-abx271044 |
Abbexa |
-
EUR 376.80
-
EUR 243.60
-
EUR 895.20
-
EUR 477.60
-
EUR 326.40
|
- 100 ug
- 10 ug
- 1 mg
- 200 ug
- 50 ug
|
|
Mecasermin protein saved stability at 4°C for as much as 2 years. The quantitative and qualitative management indicated that Mecasermin is expressed appropriately (with out the preliminary methionine by Mass spectrometry), pure (with out endotoxin and different protein impurities), appropriate folding (FTIR, RF-HPLC), monomer type (SEC-HPLC) and lively (bioactivity check). Additionally, the purification outcomes revealed that expression in low temperature outcomes the environment friendly purification of the over-produced Mecasermin with a excessive amount and high quality.
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