Recombinant production of active microbial transglutaminase in E. coli by using self-cleavable zymogen with mutated propeptide

Microbial transglutaminase from Streptomyces mobaraensis (MTG) has been extensively utilized in meals business and in addition in analysis and medical functions, since it may possibly site-specifically modify proteins by the cross-linking response of glutamine residue and the first amino group. The recombinant expression system of MTG in E. coli supplies higher accessibility for the researchers and thus can promote additional utilization of MTG. Herein, we report manufacturing of lively and soluble MTG in E. coli through the use of a chimeric protein of tobacco etch virus (TEV) protease and MTG zymogen.

A chimera of TEV protease and MTG zymogen with native propeptide resulted in lively MTG contaminated with cleaved propeptide because of the robust interplay between the propeptide and catalytic area of MTG. Introduction of mutations of K9R and Y11A to the propeptide facilitated dissociation of the cleaved propeptide from the catalytic area of MTG and lively MTG with none contamination of the propeptide was obtained. The particular exercise of the lively MTG was 22.7±2.6 U/mg. The profitable expression and purification of lively MTG through the use of the chimera protein of TEV protease and MTG zymogen with mutations within the propeptide can advance using MTG and the researches utilizing MTG mediated cross-linking reactions.

Technology of novel trimeric fragments of human SP-A and SP-D after recombinant soluble expression in E. coli

Surfactant therapy for neonatal respiratory misery syndrome has dramatically improved survival of preterm infants. Nonetheless, this has resulted in a markedly elevated incidence of sequelae corresponding to neonatal persistent inflammatory lung illness. The present surfactant preparations in medical use lack the pure lung defence proteins surfactant proteins (SP)-A and D. These are identified to have anti-inflammatory and anti-infective properties important for sustaining wholesome non-inflamed lungs.

Supplementation of at the moment accessible animal derived surfactant therapeutics with these anti-inflammatory proteins within the first few days of life may stop the event of inflammatory lung illness in untimely infants. Nonetheless, present methods for manufacturing of recombinant variations of SP-A and SP-D require a posh solubilisation and refolding protocol limiting expression at scale for drug improvement. Utilizing a novel solubility tag, we describe the expression and purification of recombinant fragments of human (rfh) SP-A and SP-D utilizing Escherichia coli with out the necessity for refolding.

We obtained a imply (± SD) of 23.3 (± 5.4) mg and 86 mg (± 3.5) per litre yield of rfhSP-A and rfhSP-D, respectively. rfhSP-D was trimeric and 68% sure to a ManNAc-affinity column, giving a closing yield of 57.5 mg/litre of extremely pure protein, considerably increased than the three.Three mg/litre obtained via the usual refolding protocol. Additional optimisation of this novel lab primarily based methodology may doubtlessly make rfhSP-A and rfhSP-D manufacturing extra commercially possible to allow improvement of novel therapeutics for the therapy of lung an infection and irritation.

Expression, solubilization, refolding and closing purification of recombinant proteins as expressed within the type of “classical inclusion our bodies” in E. coli

Escherichia coli has been most generally used for manufacturing of the recombinant proteins. Over-expression of the recombinant proteins is the mainspring of the inclusion our bodies formation. The refolding of those proteins into bioactive types is cumbersome and partly time-consuming. Within the current examine, we reviewed and mentioned most points concerning the restoration of "classical inclusion our bodies" by specializing in our earlier experiences.

Performing correct strategies of expression, solubilization, refolding and closing purification of those proteins, would make it attainable to get well increased quantities of pro-teins into the native type with applicable conformation. Usually, offering gentle circumstances and correct refolding buffers, would result in get well greater than 40% of inclusion our bodies into bioactive and native conformation.

Efficient Course of Growth for Excessive-level Manufacturing, Purification, Formulation, and Characterization of Recombinant Mecasermin in E. coli

Over-production recombinant Mecasermin was achieved by investigation impact of three components, temperature, inducer quantity and tradition media in three ranges in accordance with Taguchi statistical design in E. coli in a bench-scale bioreactor. In optimum circumstances (induction temperature 28°C, TB+G medium, with 0.1 mM IPTG as inducer) 0.84 g/L Mecasermin with expression ranges of 38% of whole protein and 4.13 g/L closing dry cell biomass was produced, that is likely one of the highest values recombinant protein has been reported within the batch system.

The cell disruption was executed by lysozyme pretreatment with sonication to the environment friendly purification of Mecasermin. The remoted and washed inclusion our bodies had been solubilized in Gdn-HCl at pH 5.Four and folded with glutathione and purified with gel filtration. The purified rhIGF-1 (Mecasermin) was formulated with arginine.

Porcine Connective Tissue Growth Factor (CTGF) ELISA Kit
DLR-CTGF-p-48T	DL Develop	48T	EUR 591.6
Description: A sandwich quantitative ELISA assay kit for detection of Porcine Connective Tissue Growth Factor (CTGF) in samples from serum, plasma, tissue homogenates or other biological fluids.

Porcine Connective Tissue Growth Factor (CTGF) ELISA Kit
DLR-CTGF-p-96T	DL Develop	96T	EUR 769.2
Description: A sandwich quantitative ELISA assay kit for detection of Porcine Connective Tissue Growth Factor (CTGF) in samples from serum, plasma, tissue homogenates or other biological fluids.

Rat Connective Tissue Growth Factor (CTGF) ELISA Kit
DLR-CTGF-Ra-48T	DL Develop	48T	EUR 544.8
Description: A sandwich quantitative ELISA assay kit for detection of Rat Connective Tissue Growth Factor (CTGF) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Rat Connective Tissue Growth Factor (CTGF) ELISA Kit
DLR-CTGF-Ra-96T	DL Develop	96T	EUR 704.4
Description: A sandwich quantitative ELISA assay kit for detection of Rat Connective Tissue Growth Factor (CTGF) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Rabbit Connective Tissue Growth Factor (CTGF) ELISA Kit
DLR-CTGF-Rb-48T	DL Develop	48T	EUR 544.8
Description: A sandwich quantitative ELISA assay kit for detection of Rabbit Connective Tissue Growth Factor (CTGF) in samples from serum, plasma or other biological fluids.

Rabbit Connective Tissue Growth Factor (CTGF) ELISA Kit
DLR-CTGF-Rb-96T	DL Develop	96T	EUR 704.4
Description: A sandwich quantitative ELISA assay kit for detection of Rabbit Connective Tissue Growth Factor (CTGF) in samples from serum, plasma or other biological fluids.

Bovine Connective Tissue Growth Factor (CTGF) ELISA Kit
RD-CTGF-b-48Tests	Reddot Biotech	48 Tests	EUR 592.8

Bovine Connective Tissue Growth Factor (CTGF) ELISA Kit
RD-CTGF-b-96Tests	Reddot Biotech	96 Tests	EUR 820.8

Human Connective Tissue Growth Factor (CTGF) ELISA Kit
RD-CTGF-Hu-48Tests	Reddot Biotech	48 Tests	EUR 450

Human Connective Tissue Growth Factor (CTGF) ELISA Kit
RD-CTGF-Hu-96Tests	Reddot Biotech	96 Tests	EUR 618

Mouse Connective Tissue Growth Factor (CTGF) ELISA Kit
RD-CTGF-Mu-48Tests	Reddot Biotech	48 Tests	EUR 514.8

Mouse Connective Tissue Growth Factor (CTGF) ELISA Kit
RD-CTGF-Mu-96Tests	Reddot Biotech	96 Tests	EUR 709.2

Porcine Connective Tissue Growth Factor (CTGF) ELISA Kit
RD-CTGF-p-48Tests	Reddot Biotech	48 Tests	EUR 592.8

Porcine Connective Tissue Growth Factor (CTGF) ELISA Kit
RD-CTGF-p-96Tests	Reddot Biotech	96 Tests	EUR 820.8

Rat Connective Tissue Growth Factor (CTGF) ELISA Kit
RD-CTGF-Ra-48Tests	Reddot Biotech	48 Tests	EUR 540

Rat Connective Tissue Growth Factor (CTGF) ELISA Kit
RD-CTGF-Ra-96Tests	Reddot Biotech	96 Tests	EUR 746.4

Rabbit Connective Tissue Growth Factor (CTGF) ELISA Kit
RD-CTGF-Rb-48Tests	Reddot Biotech	48 Tests	EUR 540

Rabbit Connective Tissue Growth Factor (CTGF) ELISA Kit
RD-CTGF-Rb-96Tests	Reddot Biotech	96 Tests	EUR 746.4

Bovine Connective Tissue Growth Factor (CTGF) ELISA Kit
RDR-CTGF-b-48Tests	Reddot Biotech	48 Tests	EUR 619.2

Bovine Connective Tissue Growth Factor (CTGF) ELISA Kit
RDR-CTGF-b-96Tests	Reddot Biotech	96 Tests	EUR 859.2

Chicken Connective Tissue Growth Factor (CTGF) ELISA Kit
RDR-CTGF-Ch-48Tests	Reddot Biotech	48 Tests	EUR 619.2

Chicken Connective Tissue Growth Factor (CTGF) ELISA Kit
RDR-CTGF-Ch-96Tests	Reddot Biotech	96 Tests	EUR 859.2

Human Connective Tissue Growth Factor (CTGF) ELISA Kit
RDR-CTGF-Hu-48Tests	Reddot Biotech	48 Tests	EUR 469.2

Human Connective Tissue Growth Factor (CTGF) ELISA Kit
RDR-CTGF-Hu-96Tests	Reddot Biotech	96 Tests	EUR 645.6

Mouse Connective Tissue Growth Factor (CTGF) ELISA Kit
RDR-CTGF-Mu-48Tests	Reddot Biotech	48 Tests	EUR 536.4

Mouse Connective Tissue Growth Factor (CTGF) ELISA Kit
RDR-CTGF-Mu-96Tests	Reddot Biotech	96 Tests	EUR 741.6

Porcine Connective Tissue Growth Factor (CTGF) ELISA Kit
RDR-CTGF-p-48Tests	Reddot Biotech	48 Tests	EUR 619.2

Porcine Connective Tissue Growth Factor (CTGF) ELISA Kit
RDR-CTGF-p-96Tests	Reddot Biotech	96 Tests	EUR 859.2

Rat Connective Tissue Growth Factor (CTGF) ELISA Kit
RDR-CTGF-Ra-48Tests	Reddot Biotech	48 Tests	EUR 564

Rat Connective Tissue Growth Factor (CTGF) ELISA Kit
RDR-CTGF-Ra-96Tests	Reddot Biotech	96 Tests	EUR 781.2

Rabbit Connective Tissue Growth Factor (CTGF) ELISA Kit
RDR-CTGF-Rb-48Tests	Reddot Biotech	48 Tests	EUR 564

Rabbit Connective Tissue Growth Factor (CTGF) ELISA Kit
RDR-CTGF-Rb-96Tests	Reddot Biotech	96 Tests	EUR 781.2

Pig Connective Tissue Growth Factor (CTGF) Protein
20-abx066093	Abbexa	EUR 878.40 EUR 343.20 EUR 2766.00 EUR 1062.00 EUR 627.60	100 ug 10 ug 1 mg 200 ug 50 ug

Connective Tissue Growth Factor (CTGF) Antibody
20-abx171909	Abbexa	EUR 1011.60 EUR 526.80	1 mg 200 ug

Connective Tissue Growth Factor (CTGF) Antibody
20-abx171910	Abbexa	EUR 1095.60 EUR 560.40	1 mg 200 ug

Connective Tissue Growth Factor (CTGF) Antibody
20-abx132193	Abbexa	EUR 510.00 EUR 159.60 EUR 1412.40 EUR 693.60 EUR 393.60	100 ug 10 ug 1 mg 200 ug 50 ug

Connective Tissue Growth Factor (CTGF) Antibody
20-abx132350	Abbexa	EUR 393.60 EUR 978.00 EUR 510.00 EUR 184.80 EUR 309.60	100 ug 1 mg 200 ug 20 ug 50 ug

Connective Tissue Growth Factor (CTGF) Antibody
20-abx132360	Abbexa	EUR 393.60 EUR 994.80 EUR 510.00 EUR 184.80 EUR 309.60	100 ug 1 mg 200 ug 20 ug 50 ug

Connective Tissue Growth Factor (CTGF) Antibody
20-abx104836	Abbexa	EUR 543.60 EUR 159.60 EUR 1545.60 EUR 744.00 EUR 410.40	100 ug 10 ug 1 mg 200 ug 50 ug

Connective Tissue Growth Factor (CTGF) Antibody
20-abx103225	Abbexa	EUR 427.20 EUR 159.60 EUR 1195.20 EUR 594.00 EUR 360.00	100 ug 10 ug 1 mg 200 ug 50 ug

Connective Tissue Growth Factor (CTGF) Antibody
20-abx103226	Abbexa	EUR 427.20 EUR 159.60 EUR 1195.20 EUR 594.00 EUR 360.00	100 ug 10 ug 1 mg 200 ug 50 ug

Connective Tissue Growth Factor (CTGF) Antibody
20-abx103227	Abbexa	EUR 360.00 EUR 861.60 EUR 460.80 EUR 184.80 EUR 292.80	100 ug 1 mg 200 ug 20 ug 50 ug

Connective Tissue Growth Factor (CTGF) Antibody
20-abx103228	Abbexa	EUR 493.20 EUR 159.60 EUR 1362.00 EUR 661.20 EUR 376.80	100 ug 10 ug 1 mg 200 ug 50 ug

Connective Tissue Growth Factor (CTGF) Antibody
20-abx103229	Abbexa	EUR 510.00 EUR 159.60 EUR 1412.40 EUR 693.60 EUR 393.60	100 ug 10 ug 1 mg 200 ug 50 ug

Connective Tissue Growth Factor (CTGF) Antibody
20-abx103230	Abbexa	EUR 543.60 EUR 159.60 EUR 1545.60 EUR 744.00 EUR 410.40	100 ug 10 ug 1 mg 200 ug 50 ug

Connective Tissue Growth Factor (CTGF) Antibody
20-abx103231	Abbexa	EUR 560.40 EUR 159.60 EUR 1646.40 EUR 777.60 EUR 427.20	100 ug 10 ug 1 mg 200 ug 50 ug

Connective Tissue Growth Factor (CTGF) Antibody
abx037800-100ug	Abbexa	100 ug	EUR 469.2

Connective Tissue Growth Factor (CTGF) Antibody
20-abx123700	Abbexa	EUR 493.20 EUR 710.40	100 ul 200 ul

Connective Tissue Growth Factor (CTGF) Antibody
20-abx109670	Abbexa	EUR 493.20 EUR 2214.00 EUR 718.80 EUR 218.40 EUR 360.00	100 ug 1 mg 200 ug 20 ug 50 ug

Connective Tissue Growth Factor (CTGF) Antibody
20-abx131768	Abbexa	EUR 393.60 EUR 1011.60 EUR 526.80 EUR 184.80 EUR 309.60	100 ug 1 mg 200 ug 20 ug 50 ug

Connective Tissue Growth Factor (CTGF) Antibody
20-abx125726	Abbexa	EUR 493.20 EUR 710.40 EUR 218.40 EUR 376.80	100 ul 200 ul 20 ul 50 ul

Connective Tissue Growth Factor (CTGF) Antibody
20-abx125727	Abbexa	EUR 493.20 EUR 710.40 EUR 218.40 EUR 376.80	100 ul 200 ul 20 ul 50 ul

Connective Tissue Growth Factor (CTGF) Antibody
20-abx125728	Abbexa	EUR 493.20 EUR 710.40 EUR 376.80	100 ul 200 ul 50 ul

Connective Tissue Growth Factor (CTGF) Antibody
abx033675-400ul	Abbexa	400 ul	EUR 627.6

Connective Tissue Growth Factor (CTGF) Antibody
abx033675-80l	Abbexa	80 µl	EUR 343.2

Connective Tissue Growth Factor (CTGF) Antibody
abx033676-400ul	Abbexa	400 ul	EUR 627.6

Connective Tissue Growth Factor (CTGF) Antibody
abx033676-80l	Abbexa	80 µl	EUR 343.2

Connective Tissue Growth Factor (CTGF) Antibody
abx027387-400ul	Abbexa	400 ul	EUR 627.6

Connective Tissue Growth Factor (CTGF) Antibody
abx027387-80l	Abbexa	80 µl	EUR 343.2

Connective Tissue Growth Factor (CTGF) Antibody
abx025473-100ul	Abbexa	100 ul	EUR 627.6

Connective Tissue Growth Factor (CTGF) Antibody
20-abx225128	Abbexa	EUR 444.00 EUR 727.20 EUR 376.80	100 ul 200 ul 50 ul

Connective Tissue Growth Factor (Ctgf) Antibody
20-abx319302	Abbexa	EUR 493.20 EUR 2214.00 EUR 718.80 EUR 218.40 EUR 360.00	100 ug 1 mg 200 ug 20 ug 50 ug

Connective Tissue Growth Factor (CTGF) Antibody
abx433502-200ul	Abbexa	200 ul	EUR 543.6

Connective Tissue Growth Factor (CTGF) Antibody
abx232053-100ug	Abbexa	100 ug	EUR 610.8

Connective Tissue Growth Factor (CTGF) Antibody
abx232054-100ug	Abbexa	100 ug	EUR 610.8

Connective Tissue Growth Factor (CTGF) Antibody
20-abx175995	Abbexa	EUR 1362.00 EUR 661.20	1 mg 200 ug

Connective Tissue Growth Factor (CTGF) Antibody
20-abx175996	Abbexa	EUR 1412.40 EUR 693.60	1 mg 200 ug

Connective Tissue Growth Factor (CTGF) Antibody
20-abx175997	Abbexa	EUR 1579.20 EUR 760.80	1 mg 200 ug

Connective tissue growth factor (CTGF) Antibody
20-abx214776	Abbexa	EUR 493.20 EUR 360.00	100 ul 50 ul

Active Connective Tissue Growth Factor (CTGF)
4-APA010Ra01	Cloud-Clone	EUR 797.38 EUR 337.20 EUR 2660.16 EUR 966.72 EUR 1813.44 EUR 609.60 EUR 6470.40	100 ug 10ug 1 mg 200 ug 500 ug 50ug 5 mg
Description: Recombinant Rat Connective Tissue Growth Factor expressed in: Available from E.coli, Yeast, Baculovirus and Mammalian cells

Human Connective tissue growth factor (CTGF)
1-CSB-EP006147HU	Cusabio	EUR 606.00 EUR 318.00 EUR 2192.40 EUR 919.20 EUR 1461.60 EUR 402.00	100ug 10ug 1MG 200ug 500ug 50ug
Description: Recombinant Human Connective tissue growth factor(CTGF) expressed in E.coli

Human Connective tissue growth factor (CTGF)
1-CSB-EP006147HU1	Cusabio	EUR 456.00 EUR 256.80 EUR 1570.80 EUR 672.00 EUR 1047.60 EUR 314.40	100ug 10ug 1MG 200ug 500ug 50ug
Description: Recombinant Human Connective tissue growth factor(CTGF),partial expressed in E.coli

Mouse Connective tissue growth factor (Ctgf)
1-CSB-EP006147MO	Cusabio	EUR 606.00 EUR 318.00 EUR 2192.40 EUR 919.20 EUR 1461.60 EUR 402.00	100ug 10ug 1MG 200ug 500ug 50ug
Description: Recombinant Mouse Connective tissue growth factor (Ctgf),partial expressed in E.coli

Mouse Connective tissue growth factor (Ctgf)
1-CSB-EP006147MOe0	Cusabio	EUR 606.00 EUR 318.00 EUR 2192.40 EUR 919.20 EUR 1461.60 EUR 402.00	100ug 10ug 1MG 200ug 500ug 50ug
Description: Recombinant Mouse Connective tissue growth factor(Ctgf),partial expressed in E.coli

Recombinant Connective Tissue Growth Factor (CTGF)
4-RPA010Bo01	Cloud-Clone	EUR 636.10 EUR 294.00 EUR 2055.36 EUR 765.12 EUR 1410.24 EUR 501.60 EUR 4958.40	100 ug 10ug 1 mg 200 ug 500 ug 50ug 5 mg
Description: Recombinant Bovine Connective Tissue Growth Factor expressed in: E.coli

Recombinant Connective Tissue Growth Factor (CTGF)
4-RPA010Hu01	Cloud-Clone	EUR 571.58 EUR 276.00 EUR 1813.44 EUR 684.48 EUR 1248.96 EUR 458.40 EUR 4353.60	100 ug 10ug 1 mg 200 ug 500 ug 50ug 5 mg
Description: Recombinant Human Connective Tissue Growth Factor expressed in: E.coli

Recombinant Connective Tissue Growth Factor (CTGF)
4-RPA010Hu02	Cloud-Clone	EUR 571.58 EUR 276.00 EUR 1813.44 EUR 684.48 EUR 1248.96 EUR 458.40 EUR 4353.60	100 ug 10ug 1 mg 200 ug 500 ug 50ug 5 mg
Description: Recombinant Human Connective Tissue Growth Factor expressed in: E.coli

Recombinant Connective Tissue Growth Factor (CTGF)
4-RPA010Mu01	Cloud-Clone	EUR 603.84 EUR 285.60 EUR 1934.40 EUR 724.80 EUR 1329.60 EUR 480.00 EUR 4656.00	100 ug 10ug 1 mg 200 ug 500 ug 50ug 5 mg
Description: Recombinant Mouse Connective Tissue Growth Factor expressed in: E.coli

Recombinant Connective Tissue Growth Factor (CTGF)
4-RPA010Ov01	Cloud-Clone	EUR 668.35 EUR 302.40 EUR 2176.32 EUR 805.44 EUR 1490.88 EUR 523.20 EUR 5260.80	100 ug 10ug 1 mg 200 ug 500 ug 50ug 5 mg
Description: Recombinant Sheep Connective Tissue Growth Factor expressed in: E.coli

Recombinant Connective Tissue Growth Factor (CTGF)
4-RPA010Po01	Cloud-Clone	EUR 636.10 EUR 294.00 EUR 2055.36 EUR 765.12 EUR 1410.24 EUR 501.60 EUR 4958.40	100 ug 10ug 1 mg 200 ug 500 ug 50ug 5 mg
Description: Recombinant Pig Connective Tissue Growth Factor expressed in: E.coli

Recombinant Connective Tissue Growth Factor (CTGF)
4-RPA010Ra01	Cloud-Clone	EUR 496.32 EUR 256.80 EUR 1531.20 EUR 590.40 EUR 1060.80 EUR 408.00 EUR 3648.00	100 ug 10ug 1 mg 200 ug 500 ug 50ug 5 mg
Description: Recombinant Rat Connective Tissue Growth Factor expressed in: E.coli

Pig Connective Tissue Growth Factor (CTGF) ELISA Kit
20-abx154906	Abbexa	EUR 9174.00 EUR 4888.80 EUR 1130.40	10 × 96 tests 5 × 96 tests 96 tests

Pig Connective Tissue Growth Factor (CTGF) ELISA Kit
abx573322-96tests	Abbexa	96 tests	EUR 990

Pig Connective Tissue Growth Factor (CTGF) CLIA Kit
20-abx190822	Abbexa	EUR 11090.40 EUR 5904.00 EUR 1356.00	10 × 96 tests 5 × 96 tests 96 tests

Connective Tissue Growth Factor (CTGF) Polyclonal Antibody (Pig)
4-PAA010Po01	Cloud-Clone	EUR 310.80 EUR 3232.80 EUR 800.40 EUR 391.20 EUR 261.60	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Pig Connective Tissue Growth Factor (CTGF)

Pig Connective Tissue Growth Factor (CTGF) ELISA Kit
SEA010Po-10x96wellstestplate	Cloud-Clone	10x96-wells test plate	EUR 6117.62
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Pig Connective Tissue Growth Factor (CTGF) in serum, plasma, tissue homogenates and other biological fluids.

Pig Connective Tissue Growth Factor (CTGF) ELISA Kit
SEA010Po-1x48wellstestplate	Cloud-Clone	1x48-wells test plate	EUR 608.98
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Pig Connective Tissue Growth Factor (CTGF) in serum, plasma, tissue homogenates and other biological fluids.

Pig Connective Tissue Growth Factor (CTGF) ELISA Kit
SEA010Po-1x96wellstestplate	Cloud-Clone	1x96-wells test plate	EUR 818.54
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Pig Connective Tissue Growth Factor (CTGF) in serum, plasma, tissue homogenates and other biological fluids.

Pig Connective Tissue Growth Factor (CTGF) ELISA Kit
SEA010Po-5x96wellstestplate	Cloud-Clone	5x96-wells test plate	EUR 3323.45
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Pig Connective Tissue Growth Factor (CTGF) in serum, plasma, tissue homogenates and other biological fluids.

Pig Connective Tissue Growth Factor (CTGF) ELISA Kit
4-SEA010Po	Cloud-Clone	EUR 6178.80 EUR 3264.00 EUR 819.60	10 plates of 96 wells 5 plates of 96 wells 1 plate of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Pig Connective Tissue Growth Factor (CTGF) in samples from Serum, plasma, tissue homogenates and other biological fluids. with no significant corss-reactivity with analogues from other species.

Pig Connective Tissue Growth Factor (CTGF)CLIA Kit
SCA010Po-10x96wellstestplate	Cloud-Clone	10x96-wells test plate	EUR 8096.83
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Pig Connective Tissue Growth Factor (CTGF) in serum, plasma and other biological fluids.

Pig Connective Tissue Growth Factor (CTGF)CLIA Kit
SCA010Po-1x48wellstestplate	Cloud-Clone	1x48-wells test plate	EUR 771.97
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Pig Connective Tissue Growth Factor (CTGF) in serum, plasma and other biological fluids.

Pig Connective Tissue Growth Factor (CTGF)CLIA Kit
SCA010Po-1x96wellstestplate	Cloud-Clone	1x96-wells test plate	EUR 1051.39
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Pig Connective Tissue Growth Factor (CTGF) in serum, plasma and other biological fluids.

Pig Connective Tissue Growth Factor (CTGF)CLIA Kit
SCA010Po-5x96wellstestplate	Cloud-Clone	5x96-wells test plate	EUR 4371.26
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Pig Connective Tissue Growth Factor (CTGF) in serum, plasma and other biological fluids.

Pig Connective Tissue Growth Factor (CTGF) CLIA Kit
4-SCA010Po	Cloud-Clone	EUR 8157.60 EUR 4371.60 EUR 1052.40	10 plates of 96 wells 5 plates of 96 wells 1 plate of 96 wells
Description: Double-antibody Sandwich chemiluminescent immunoassay for detection of Pig Connective Tissue Growth Factor (CTGF)Serum, plasma and other biological fluids

CLIA kit for Human CTGF (Connective Tissue Growth Factor)
E-CL-H0579	Elabscience Biotech	1 plate of 96 wells	EUR 700.8
Description: A sandwich CLIA kit for quantitative measurement of Human CTGF (Connective Tissue Growth Factor) in samples from Serum, Plasma, Cell supernatant

CLIA kit for Mouse CTGF (Connective Tissue Growth Factor)
E-CL-M0220	Elabscience Biotech	1 plate of 96 wells	EUR 700.8
Description: A sandwich CLIA kit for quantitative measurement of Mouse CTGF (Connective Tissue Growth Factor) in samples from Serum, Plasma, Cell supernatant

CLIA kit for Rat CTGF (Connective Tissue Growth Factor)
E-CL-R0173	Elabscience Biotech	1 plate of 96 wells	EUR 700.8
Description: A sandwich CLIA kit for quantitative measurement of Rat CTGF (Connective Tissue Growth Factor) in samples from Serum, Plasma, Cell supernatant

ELISA kit for Human CTGF (Connective Tissue Growth Factor)
E-EL-H0828	Elabscience Biotech	1 plate of 96 wells	EUR 640.8
Description: A sandwich ELISA kit for quantitative measurement of Human CTGF (Connective Tissue Growth Factor) in samples from Serum, Plasma, Cell supernatant

ELISA kit for Mouse CTGF (Connective Tissue Growth Factor)
E-EL-M0340	Elabscience Biotech	1 plate of 96 wells	EUR 640.8
Description: A sandwich ELISA kit for quantitative measurement of Mouse CTGF (Connective Tissue Growth Factor) in samples from Serum, Plasma, Cell supernatant

ELISA kit for Rat CTGF (Connective Tissue Growth Factor)
E-EL-R0259	Elabscience Biotech	1 plate of 96 wells	EUR 640.8
Description: A sandwich ELISA kit for quantitative measurement of Rat CTGF (Connective Tissue Growth Factor) in samples from Serum, Plasma, Cell supernatant

Guinea pig Connective Tissue Growth Factor (CTGF) ELISA Kit
abx358103-96tests	Abbexa	96 tests	EUR 990

ELISA kit for Pig CTGF (Connective Tissue Growth Factor)
ELK5960	ELK Biotech	1 plate of 96 wells	EUR 631.2
Description: A sandwich ELISA kit for detection of Connective Tissue Growth Factor from Pig in samples from blood, serum, plasma, cell culture fluid and other biological fluids.

Connective Tissue Growth Factor (CTGF) Polyclonal Antibody (Pig), APC
4-PAA010Po01-APC	Cloud-Clone	EUR 435.60 EUR 4232.40 EUR 1170.00 EUR 558.00 EUR 271.20	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Pig Connective Tissue Growth Factor (CTGF). This antibody is labeled with APC.

Connective Tissue Growth Factor (CTGF) Polyclonal Antibody (Pig), Biotinylated
4-PAA010Po01-Biotin	Cloud-Clone	EUR 388.80 EUR 3172.80 EUR 927.60 EUR 478.80 EUR 268.80	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Pig Connective Tissue Growth Factor (CTGF). This antibody is labeled with Biotin.

Connective Tissue Growth Factor (CTGF) Polyclonal Antibody (Pig), Cy3
4-PAA010Po01-Cy3	Cloud-Clone	EUR 531.60 EUR 5593.20 EUR 1510.80 EUR 693.60 EUR 312.00	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Pig Connective Tissue Growth Factor (CTGF). This antibody is labeled with Cy3.

Connective Tissue Growth Factor (CTGF) Polyclonal Antibody (Pig), FITC
4-PAA010Po01-FITC	Cloud-Clone	EUR 372.00 EUR 3409.20 EUR 960.00 EUR 470.40 EUR 241.20	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Pig Connective Tissue Growth Factor (CTGF). This antibody is labeled with FITC.

Connective Tissue Growth Factor (CTGF) Polyclonal Antibody (Pig), HRP
4-PAA010Po01-HRP	Cloud-Clone	EUR 397.20 EUR 3687.60 EUR 1034.40 EUR 502.80 EUR 255.60	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Pig Connective Tissue Growth Factor (CTGF). This antibody is labeled with HRP.

Connective Tissue Growth Factor (CTGF) Polyclonal Antibody (Pig), PE
4-PAA010Po01-PE	Cloud-Clone	EUR 372.00 EUR 3409.20 EUR 960.00 EUR 470.40 EUR 241.20	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Pig Connective Tissue Growth Factor (CTGF). This antibody is labeled with PE.

Human Connective Tissue Growth Factor (CTGF) Antibody
35148-05111	AssayPro	150 ug	EUR 313.2

Cow Connective Tissue Growth Factor (CTGF) Protein
20-abx168009	Abbexa	EUR 878.40 EUR 343.20 EUR 2766.00 EUR 1062.00 EUR 627.60	100 ug 10 ug 1 mg 200 ug 50 ug

Human Connective Tissue Growth Factor (CTGF) Protein
20-abx066091	Abbexa	EUR 794.40 EUR 326.40 EUR 2448.00 EUR 944.40 EUR 577.20	100 ug 10 ug 1 mg 200 ug 50 ug

Mouse Connective Tissue Growth Factor (CTGF) Protein
20-abx066092	Abbexa	EUR 844.80 EUR 343.20 EUR 2598.00 EUR 994.80 EUR 594.00	100 ug 10 ug 1 mg 200 ug 50 ug

Rat Connective Tissue Growth Factor (CTGF) Protein
20-abx066094	Abbexa	EUR 693.60 EUR 309.60 EUR 2064.00 EUR 828.00 EUR 510.00	100 ug 10 ug 1 mg 200 ug 50 ug

Human Connective Tissue Growth Factor (CTGF) Protein
20-abx066095	Abbexa	EUR 794.40 EUR 326.40 EUR 2448.00 EUR 944.40 EUR 577.20	100 ug 10 ug 1 mg 200 ug 50 ug

Sheep Connective Tissue Growth Factor (CTGF) Protein
20-abx066096	Abbexa	EUR 927.60 EUR 360.00 EUR 2932.80 EUR 1111.20 EUR 661.20	100 ug 10 ug 1 mg 200 ug 50 ug

Connective tissue growth factor (CTGF) Antibody (Biotin)
20-abx105285	Abbexa	EUR 493.20 EUR 2214.00 EUR 718.80 EUR 218.40 EUR 360.00	100 ug 1 mg 200 ug 20 ug 50 ug

Connective tissue growth factor (CTGF) Antibody (HRP)
20-abx108121	Abbexa	EUR 493.20 EUR 2214.00 EUR 718.80 EUR 218.40 EUR 360.00	100 ug 1 mg 200 ug 20 ug 50 ug

Connective tissue growth factor (CTGF) Antibody (FITC)
20-abx106704	Abbexa	EUR 493.20 EUR 2214.00 EUR 718.80 EUR 218.40 EUR 360.00	100 ug 1 mg 200 ug 20 ug 50 ug

Connective Tissue Growth Factor (CTGF) Antibody Pair
abx117594-1pair5x96wellplates	Abbexa	1 pair (5x96 well plates)	EUR 1212

Connective Tissue Growth Factor (CTGF) Antibody (FITC)
20-abx271026	Abbexa	EUR 376.80 EUR 243.60 EUR 944.40 EUR 493.20 EUR 326.40	100 ug 10 ug 1 mg 200 ug 50 ug

Connective Tissue Growth Factor (CTGF) Antibody (Biotin)
20-abx271044	Abbexa	EUR 376.80 EUR 243.60 EUR 895.20 EUR 477.60 EUR 326.40	100 ug 10 ug 1 mg 200 ug 50 ug

Mecasermin protein saved stability at 4°C for as much as 2 years. The quantitative and qualitative management indicated that Mecasermin is expressed appropriately (with out the preliminary methionine by Mass spectrometry), pure (with out endotoxin and different protein impurities), appropriate folding (FTIR, RF-HPLC), monomer type (SEC-HPLC) and lively (bioactivity check). Additionally, the purification outcomes revealed that expression in low temperature outcomes the environment friendly purification of the over-produced Mecasermin with a excessive amount and high quality.