Microbial transglutaminase from Streptomyces mobaraensis (MTG) has been extensively utilized in meals business and in addition in analysis and medical functions, since it may possibly site-specifically modify proteins by the cross-linking response of glutamine residue and the first amino group. The recombinant expression system of MTG in E. coli supplies higher accessibility for the researchers and thus can promote additional utilization of MTG. Herein, we report manufacturing of lively and soluble MTG in E. coli through the use of a chimeric protein of tobacco etch virus (TEV) protease and MTG zymogen.
A chimera of TEV protease and MTG zymogen with native propeptide resulted in lively MTG contaminated with cleaved propeptide because of the robust interplay between the propeptide and catalytic area of MTG. Introduction of mutations of K9R and Y11A to the propeptide facilitated dissociation of the cleaved propeptide from the catalytic area of MTG and lively MTG with none contamination of the propeptide was obtained. The particular exercise of the lively MTG was 22.7±2.6 U/mg. The profitable expression and purification of lively MTG through the use of the chimera protein of TEV protease and MTG zymogen with mutations within the propeptide can advance using MTG and the researches utilizing MTG mediated cross-linking reactions.
Technology of novel trimeric fragments of human SP-A and SP-D after recombinant soluble expression in E. coli
Surfactant therapy for neonatal respiratory misery syndrome has dramatically improved survival of preterm infants. Nonetheless, this has resulted in a markedly elevated incidence of sequelae corresponding to neonatal persistent inflammatory lung illness. The present surfactant preparations in medical use lack the pure lung defence proteins surfactant proteins (SP)-A and D. These are identified to have anti-inflammatory and anti-infective properties important for sustaining wholesome non-inflamed lungs.
Supplementation of at the moment accessible animal derived surfactant therapeutics with these anti-inflammatory proteins within the first few days of life may stop the event of inflammatory lung illness in untimely infants. Nonetheless, present methods for manufacturing of recombinant variations of SP-A and SP-D require a posh solubilisation and refolding protocol limiting expression at scale for drug improvement. Utilizing a novel solubility tag, we describe the expression and purification of recombinant fragments of human (rfh) SP-A and SP-D utilizing Escherichia coli with out the necessity for refolding.
We obtained a imply (± SD) of 23.3 (± 5.4) mg and 86 mg (± 3.5) per litre yield of rfhSP-A and rfhSP-D, respectively. rfhSP-D was trimeric and 68% sure to a ManNAc-affinity column, giving a closing yield of 57.5 mg/litre of extremely pure protein, considerably increased than the three.Three mg/litre obtained via the usual refolding protocol. Additional optimisation of this novel lab primarily based methodology may doubtlessly make rfhSP-A and rfhSP-D manufacturing extra commercially possible to allow improvement of novel therapeutics for the therapy of lung an infection and irritation.

Expression, solubilization, refolding and closing purification of recombinant proteins as expressed within the type of “classical inclusion our bodies” in E. coli
Escherichia coli has been most generally used for manufacturing of the recombinant proteins. Over-expression of the recombinant proteins is the mainspring of the inclusion our bodies formation. The refolding of those proteins into bioactive types is cumbersome and partly time-consuming. Within the current examine, we reviewed and mentioned most points concerning the restoration of "classical inclusion our bodies" by specializing in our earlier experiences.
Performing correct strategies of expression, solubilization, refolding and closing purification of those proteins, would make it attainable to get well increased quantities of pro-teins into the native type with applicable conformation. Usually, offering gentle circumstances and correct refolding buffers, would result in get well greater than 40% of inclusion our bodies into bioactive and native conformation.
Efficient Course of Growth for Excessive-level Manufacturing, Purification, Formulation, and Characterization of Recombinant Mecasermin in E. coli
Over-production recombinant Mecasermin was achieved by investigation impact of three components, temperature, inducer quantity and tradition media in three ranges in accordance with Taguchi statistical design in E. coli in a bench-scale bioreactor. In optimum circumstances (induction temperature 28°C, TB+G medium, with 0.1 mM IPTG as inducer) 0.84 g/L Mecasermin with expression ranges of 38% of whole protein and 4.13 g/L closing dry cell biomass was produced, that is likely one of the highest values recombinant protein has been reported within the batch system.
The cell disruption was executed by lysozyme pretreatment with sonication to the environment friendly purification of Mecasermin. The remoted and washed inclusion our bodies had been solubilized in Gdn-HCl at pH 5.Four and folded with glutathione and purified with gel filtration. The purified rhIGF-1 (Mecasermin) was formulated with arginine.
Porcine Connective Tissue Growth Factor (CTGF) ELISA Kit |
DLR-CTGF-p-48T |
DL Develop |
48T |
EUR 493 |
|
Description: A sandwich quantitative ELISA assay kit for detection of Porcine Connective Tissue Growth Factor (CTGF) in samples from serum, plasma, tissue homogenates or other biological fluids. |
Porcine Connective Tissue Growth Factor (CTGF) ELISA Kit |
DLR-CTGF-p-96T |
DL Develop |
96T |
EUR 641 |
|
Description: A sandwich quantitative ELISA assay kit for detection of Porcine Connective Tissue Growth Factor (CTGF) in samples from serum, plasma, tissue homogenates or other biological fluids. |
Rat Connective Tissue Growth Factor (CTGF) ELISA Kit |
DLR-CTGF-Ra-48T |
DL Develop |
48T |
EUR 454 |
|
Description: A sandwich quantitative ELISA assay kit for detection of Rat Connective Tissue Growth Factor (CTGF) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
Rat Connective Tissue Growth Factor (CTGF) ELISA Kit |
DLR-CTGF-Ra-96T |
DL Develop |
96T |
EUR 587 |
|
Description: A sandwich quantitative ELISA assay kit for detection of Rat Connective Tissue Growth Factor (CTGF) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
Rabbit Connective Tissue Growth Factor (CTGF) ELISA Kit |
DLR-CTGF-Rb-48T |
DL Develop |
48T |
EUR 454 |
|
Description: A sandwich quantitative ELISA assay kit for detection of Rabbit Connective Tissue Growth Factor (CTGF) in samples from serum, plasma or other biological fluids. |
Rabbit Connective Tissue Growth Factor (CTGF) ELISA Kit |
DLR-CTGF-Rb-96T |
DL Develop |
96T |
EUR 587 |
|
Description: A sandwich quantitative ELISA assay kit for detection of Rabbit Connective Tissue Growth Factor (CTGF) in samples from serum, plasma or other biological fluids. |
Bovine Connective Tissue Growth Factor (CTGF) ELISA Kit |
RD-CTGF-b-48Tests |
Reddot Biotech |
48 Tests |
EUR 494 |
Bovine Connective Tissue Growth Factor (CTGF) ELISA Kit |
RD-CTGF-b-96Tests |
Reddot Biotech |
96 Tests |
EUR 684 |
Human Connective Tissue Growth Factor (CTGF) ELISA Kit |
RD-CTGF-Hu-48Tests |
Reddot Biotech |
48 Tests |
EUR 375 |
Human Connective Tissue Growth Factor (CTGF) ELISA Kit |
RD-CTGF-Hu-96Tests |
Reddot Biotech |
96 Tests |
EUR 515 |
Mouse Connective Tissue Growth Factor (CTGF) ELISA Kit |
RD-CTGF-Mu-48Tests |
Reddot Biotech |
48 Tests |
EUR 429 |
Mouse Connective Tissue Growth Factor (CTGF) ELISA Kit |
RD-CTGF-Mu-96Tests |
Reddot Biotech |
96 Tests |
EUR 591 |
Porcine Connective Tissue Growth Factor (CTGF) ELISA Kit |
RD-CTGF-p-48Tests |
Reddot Biotech |
48 Tests |
EUR 494 |
Porcine Connective Tissue Growth Factor (CTGF) ELISA Kit |
RD-CTGF-p-96Tests |
Reddot Biotech |
96 Tests |
EUR 684 |
Rat Connective Tissue Growth Factor (CTGF) ELISA Kit |
RD-CTGF-Ra-48Tests |
Reddot Biotech |
48 Tests |
EUR 450 |
Rat Connective Tissue Growth Factor (CTGF) ELISA Kit |
RD-CTGF-Ra-96Tests |
Reddot Biotech |
96 Tests |
EUR 622 |
Rabbit Connective Tissue Growth Factor (CTGF) ELISA Kit |
RD-CTGF-Rb-48Tests |
Reddot Biotech |
48 Tests |
EUR 450 |
Rabbit Connective Tissue Growth Factor (CTGF) ELISA Kit |
RD-CTGF-Rb-96Tests |
Reddot Biotech |
96 Tests |
EUR 622 |
Bovine Connective Tissue Growth Factor (CTGF) ELISA Kit |
RDR-CTGF-b-48Tests |
Reddot Biotech |
48 Tests |
EUR 516 |
Bovine Connective Tissue Growth Factor (CTGF) ELISA Kit |
RDR-CTGF-b-96Tests |
Reddot Biotech |
96 Tests |
EUR 716 |
Chicken Connective Tissue Growth Factor (CTGF) ELISA Kit |
RDR-CTGF-Ch-48Tests |
Reddot Biotech |
48 Tests |
EUR 516 |
Chicken Connective Tissue Growth Factor (CTGF) ELISA Kit |
RDR-CTGF-Ch-96Tests |
Reddot Biotech |
96 Tests |
EUR 716 |
Human Connective Tissue Growth Factor (CTGF) ELISA Kit |
RDR-CTGF-Hu-48Tests |
Reddot Biotech |
48 Tests |
EUR 391 |
Human Connective Tissue Growth Factor (CTGF) ELISA Kit |
RDR-CTGF-Hu-96Tests |
Reddot Biotech |
96 Tests |
EUR 538 |
Mouse Connective Tissue Growth Factor (CTGF) ELISA Kit |
RDR-CTGF-Mu-48Tests |
Reddot Biotech |
48 Tests |
EUR 447 |
Mouse Connective Tissue Growth Factor (CTGF) ELISA Kit |
RDR-CTGF-Mu-96Tests |
Reddot Biotech |
96 Tests |
EUR 618 |
Porcine Connective Tissue Growth Factor (CTGF) ELISA Kit |
RDR-CTGF-p-48Tests |
Reddot Biotech |
48 Tests |
EUR 516 |
Porcine Connective Tissue Growth Factor (CTGF) ELISA Kit |
RDR-CTGF-p-96Tests |
Reddot Biotech |
96 Tests |
EUR 716 |
Rat Connective Tissue Growth Factor (CTGF) ELISA Kit |
RDR-CTGF-Ra-48Tests |
Reddot Biotech |
48 Tests |
EUR 470 |
Rat Connective Tissue Growth Factor (CTGF) ELISA Kit |
RDR-CTGF-Ra-96Tests |
Reddot Biotech |
96 Tests |
EUR 651 |
Rabbit Connective Tissue Growth Factor (CTGF) ELISA Kit |
RDR-CTGF-Rb-48Tests |
Reddot Biotech |
48 Tests |
EUR 470 |
Rabbit Connective Tissue Growth Factor (CTGF) ELISA Kit |
RDR-CTGF-Rb-96Tests |
Reddot Biotech |
96 Tests |
EUR 651 |
Pig Connective Tissue Growth Factor (CTGF) Protein |
20-abx066093 |
Abbexa |
-
EUR 732.00
-
EUR 286.00
-
EUR 2305.00
-
EUR 885.00
-
EUR 523.00
|
- 100 ug
- 10 ug
- 1 mg
- 200 ug
- 50 ug
|
|
Connective Tissue Growth Factor (CTGF) Antibody |
20-abx131768 |
Abbexa |
-
EUR 328.00
-
EUR 843.00
-
EUR 439.00
-
EUR 154.00
-
EUR 258.00
|
- 100 ug
- 1 mg
- 200 ug
- 20 ug
- 50 ug
|
|
Connective Tissue Growth Factor (CTGF) Antibody |
20-abx132193 |
Abbexa |
-
EUR 425.00
-
EUR 133.00
-
EUR 1177.00
-
EUR 578.00
-
EUR 328.00
|
- 100 ug
- 10 ug
- 1 mg
- 200 ug
- 50 ug
|
|
Connective Tissue Growth Factor (CTGF) Antibody |
20-abx132350 |
Abbexa |
-
EUR 328.00
-
EUR 815.00
-
EUR 425.00
-
EUR 154.00
-
EUR 258.00
|
- 100 ug
- 1 mg
- 200 ug
- 20 ug
- 50 ug
|
|
Connective Tissue Growth Factor (CTGF) Antibody |
20-abx132360 |
Abbexa |
-
EUR 328.00
-
EUR 829.00
-
EUR 425.00
-
EUR 154.00
-
EUR 258.00
|
- 100 ug
- 1 mg
- 200 ug
- 20 ug
- 50 ug
|
|
Connective Tissue Growth Factor (CTGF) Antibody |
20-abx125726 |
Abbexa |
-
EUR 411.00
-
EUR 592.00
-
EUR 182.00
-
EUR 314.00
|
- 100 ul
- 200 ul
- 20 ul
- 50 ul
|
|
Connective Tissue Growth Factor (CTGF) Antibody |
20-abx125727 |
Abbexa |
-
EUR 411.00
-
EUR 592.00
-
EUR 182.00
-
EUR 314.00
|
- 100 ul
- 200 ul
- 20 ul
- 50 ul
|
|
Connective Tissue Growth Factor (CTGF) Antibody |
20-abx125728 |
Abbexa |
-
EUR 411.00
-
EUR 592.00
-
EUR 314.00
|
|
|
Connective Tissue Growth Factor (CTGF) Antibody |
20-abx123700 |
Abbexa |
|
|
|
Connective Tissue Growth Factor (CTGF) Antibody |
20-abx104836 |
Abbexa |
-
EUR 453.00
-
EUR 133.00
-
EUR 1288.00
-
EUR 620.00
-
EUR 342.00
|
- 100 ug
- 10 ug
- 1 mg
- 200 ug
- 50 ug
|
|
Connective Tissue Growth Factor (CTGF) Antibody |
20-abx109670 |
Abbexa |
-
EUR 411.00
-
EUR 1845.00
-
EUR 599.00
-
EUR 182.00
-
EUR 300.00
|
- 100 ug
- 1 mg
- 200 ug
- 20 ug
- 50 ug
|
|
Connective Tissue Growth Factor (CTGF) Antibody |
20-abx103225 |
Abbexa |
-
EUR 356.00
-
EUR 133.00
-
EUR 996.00
-
EUR 495.00
-
EUR 300.00
|
- 100 ug
- 10 ug
- 1 mg
- 200 ug
- 50 ug
|
|
Connective Tissue Growth Factor (CTGF) Antibody |
20-abx103226 |
Abbexa |
-
EUR 356.00
-
EUR 133.00
-
EUR 996.00
-
EUR 495.00
-
EUR 300.00
|
- 100 ug
- 10 ug
- 1 mg
- 200 ug
- 50 ug
|
|
Connective Tissue Growth Factor (CTGF) Antibody |
20-abx103227 |
Abbexa |
-
EUR 300.00
-
EUR 718.00
-
EUR 384.00
-
EUR 154.00
-
EUR 244.00
|
- 100 ug
- 1 mg
- 200 ug
- 20 ug
- 50 ug
|
|
Connective Tissue Growth Factor (CTGF) Antibody |
20-abx103228 |
Abbexa |
-
EUR 411.00
-
EUR 133.00
-
EUR 1135.00
-
EUR 551.00
-
EUR 314.00
|
- 100 ug
- 10 ug
- 1 mg
- 200 ug
- 50 ug
|
|
Connective Tissue Growth Factor (CTGF) Antibody |
20-abx103229 |
Abbexa |
-
EUR 425.00
-
EUR 133.00
-
EUR 1177.00
-
EUR 578.00
-
EUR 328.00
|
- 100 ug
- 10 ug
- 1 mg
- 200 ug
- 50 ug
|
|
Connective Tissue Growth Factor (CTGF) Antibody |
20-abx103230 |
Abbexa |
-
EUR 453.00
-
EUR 133.00
-
EUR 1288.00
-
EUR 620.00
-
EUR 342.00
|
- 100 ug
- 10 ug
- 1 mg
- 200 ug
- 50 ug
|
|
Connective Tissue Growth Factor (CTGF) Antibody |
20-abx103231 |
Abbexa |
-
EUR 467.00
-
EUR 133.00
-
EUR 1372.00
-
EUR 648.00
-
EUR 356.00
|
- 100 ug
- 10 ug
- 1 mg
- 200 ug
- 50 ug
|
|
Connective Tissue Growth Factor (CTGF) Antibody |
abx037800-100ug |
Abbexa |
100 ug |
EUR 391 |
|
Connective Tissue Growth Factor (CTGF) Antibody |
abx025473-100ul |
Abbexa |
100 ul |
EUR 523 |
|
Connective Tissue Growth Factor (CTGF) Antibody |
abx027387-400ul |
Abbexa |
400 ul |
EUR 523 |
|
Connective Tissue Growth Factor (CTGF) Antibody |
abx027387-80l |
Abbexa |
80 µl |
EUR 286 |
|
Connective Tissue Growth Factor (CTGF) Antibody |
abx033675-400ul |
Abbexa |
400 ul |
EUR 523 |
|
Connective Tissue Growth Factor (CTGF) Antibody |
abx033675-80l |
Abbexa |
80 µl |
EUR 286 |
|
Connective Tissue Growth Factor (CTGF) Antibody |
abx033676-400ul |
Abbexa |
400 ul |
EUR 523 |
|
Connective Tissue Growth Factor (CTGF) Antibody |
abx033676-80l |
Abbexa |
80 µl |
EUR 286 |
|
Connective Tissue Growth Factor (CTGF) Antibody |
abx433502-200ul |
Abbexa |
200 ul |
EUR 453 |
|
Connective Tissue Growth Factor (CTGF) Antibody |
20-abx171909 |
Abbexa |
|
|
|
Connective Tissue Growth Factor (CTGF) Antibody |
20-abx171910 |
Abbexa |
|
|
|
Connective Tissue Growth Factor (Ctgf) Antibody |
20-abx319302 |
Abbexa |
-
EUR 411.00
-
EUR 1845.00
-
EUR 599.00
-
EUR 182.00
-
EUR 300.00
|
- 100 ug
- 1 mg
- 200 ug
- 20 ug
- 50 ug
|
|
Connective Tissue Growth Factor (CTGF) Antibody |
20-abx225128 |
Abbexa |
-
EUR 370.00
-
EUR 606.00
-
EUR 314.00
|
|
|
Connective tissue growth factor (CTGF) Antibody |
20-abx214776 |
Abbexa |
|
|
|
Connective Tissue Growth Factor (CTGF) Antibody |
abx232053-100ug |
Abbexa |
100 ug |
EUR 509 |
|
Connective Tissue Growth Factor (CTGF) Antibody |
abx232054-100ug |
Abbexa |
100 ug |
EUR 509 |
|
Connective Tissue Growth Factor (CTGF) Antibody |
20-abx175995 |
Abbexa |
|
|
|
Connective Tissue Growth Factor (CTGF) Antibody |
20-abx175996 |
Abbexa |
|
|
|
Connective Tissue Growth Factor (CTGF) Antibody |
20-abx175997 |
Abbexa |
|
|
|
Human Connective tissue growth factor (CTGF) |
1-CSB-EP006147HU |
Cusabio |
-
EUR 505.00
-
EUR 265.00
-
EUR 1827.00
-
EUR 766.00
-
EUR 1218.00
-
EUR 335.00
|
- 100ug
- 10ug
- 1MG
- 200ug
- 500ug
- 50ug
|
|
Description: Recombinant Human Connective tissue growth factor(CTGF) expressed in E.coli |
Human Connective tissue growth factor (CTGF) |
1-CSB-EP006147HU1 |
Cusabio |
-
EUR 380.00
-
EUR 214.00
-
EUR 1309.00
-
EUR 560.00
-
EUR 873.00
-
EUR 262.00
|
- 100ug
- 10ug
- 1MG
- 200ug
- 500ug
- 50ug
|
|
Description: Recombinant Human Connective tissue growth factor(CTGF),partial expressed in E.coli |
Mouse Connective tissue growth factor (Ctgf) |
1-CSB-EP006147MO |
Cusabio |
-
EUR 505.00
-
EUR 265.00
-
EUR 1827.00
-
EUR 766.00
-
EUR 1218.00
-
EUR 335.00
|
- 100ug
- 10ug
- 1MG
- 200ug
- 500ug
- 50ug
|
|
Description: Recombinant Mouse Connective tissue growth factor (Ctgf),partial expressed in E.coli |
Mouse Connective tissue growth factor (Ctgf) |
1-CSB-EP006147MOe0 |
Cusabio |
-
EUR 505.00
-
EUR 265.00
-
EUR 1827.00
-
EUR 766.00
-
EUR 1218.00
-
EUR 335.00
|
- 100ug
- 10ug
- 1MG
- 200ug
- 500ug
- 50ug
|
|
Description: Recombinant Mouse Connective tissue growth factor(Ctgf),partial expressed in E.coli |
Active Connective Tissue Growth Factor (CTGF) |
4-APA010Ra01 |
Cloud-Clone |
-
EUR 664.48
-
EUR 281.00
-
EUR 2216.80
-
EUR 805.60
-
EUR 1511.20
-
EUR 508.00
-
EUR 5392.00
|
- 100 ug
- 10ug
- 1 mg
- 200 ug
- 500 ug
- 50ug
- 5 mg
|
|
Description: Recombinant Rat Connective Tissue Growth Factor expressed in: Available from E.coli, Yeast, Baculovirus and Mammalian cells |
Recombinant Connective Tissue Growth Factor (CTGF) |
4-RPA010Bo01 |
Cloud-Clone |
-
EUR 530.08
-
EUR 245.00
-
EUR 1712.80
-
EUR 637.60
-
EUR 1175.20
-
EUR 418.00
-
EUR 4132.00
|
- 100 ug
- 10ug
- 1 mg
- 200 ug
- 500 ug
- 50ug
- 5 mg
|
|
Description: Recombinant Bovine Connective Tissue Growth Factor expressed in: E.coli |
Recombinant Connective Tissue Growth Factor (CTGF) |
4-RPA010Hu01 |
Cloud-Clone |
-
EUR 476.32
-
EUR 230.00
-
EUR 1511.20
-
EUR 570.40
-
EUR 1040.80
-
EUR 382.00
-
EUR 3628.00
|
- 100 ug
- 10ug
- 1 mg
- 200 ug
- 500 ug
- 50ug
- 5 mg
|
|
Description: Recombinant Human Connective Tissue Growth Factor expressed in: E.coli |
Recombinant Connective Tissue Growth Factor (CTGF) |
4-RPA010Hu02 |
Cloud-Clone |
-
EUR 476.32
-
EUR 230.00
-
EUR 1511.20
-
EUR 570.40
-
EUR 1040.80
-
EUR 382.00
-
EUR 3628.00
|
- 100 ug
- 10ug
- 1 mg
- 200 ug
- 500 ug
- 50ug
- 5 mg
|
|
Description: Recombinant Human Connective Tissue Growth Factor expressed in: E.coli |
Recombinant Connective Tissue Growth Factor (CTGF) |
4-RPA010Mu01 |
Cloud-Clone |
-
EUR 503.20
-
EUR 238.00
-
EUR 1612.00
-
EUR 604.00
-
EUR 1108.00
-
EUR 400.00
-
EUR 3880.00
|
- 100 ug
- 10ug
- 1 mg
- 200 ug
- 500 ug
- 50ug
- 5 mg
|
|
Description: Recombinant Mouse Connective Tissue Growth Factor expressed in: E.coli |
Recombinant Connective Tissue Growth Factor (CTGF) |
4-RPA010Ov01 |
Cloud-Clone |
-
EUR 556.96
-
EUR 252.00
-
EUR 1813.60
-
EUR 671.20
-
EUR 1242.40
-
EUR 436.00
-
EUR 4384.00
|
- 100 ug
- 10ug
- 1 mg
- 200 ug
- 500 ug
- 50ug
- 5 mg
|
|
Description: Recombinant Sheep Connective Tissue Growth Factor expressed in: E.coli |
Recombinant Connective Tissue Growth Factor (CTGF) |
4-RPA010Po01 |
Cloud-Clone |
-
EUR 530.08
-
EUR 245.00
-
EUR 1712.80
-
EUR 637.60
-
EUR 1175.20
-
EUR 418.00
-
EUR 4132.00
|
- 100 ug
- 10ug
- 1 mg
- 200 ug
- 500 ug
- 50ug
- 5 mg
|
|
Description: Recombinant Pig Connective Tissue Growth Factor expressed in: E.coli |
Recombinant Connective Tissue Growth Factor (CTGF) |
4-RPA010Ra01 |
Cloud-Clone |
-
EUR 413.60
-
EUR 214.00
-
EUR 1276.00
-
EUR 492.00
-
EUR 884.00
-
EUR 340.00
-
EUR 3040.00
|
- 100 ug
- 10ug
- 1 mg
- 200 ug
- 500 ug
- 50ug
- 5 mg
|
|
Description: Recombinant Rat Connective Tissue Growth Factor expressed in: E.coli |
Pig Connective Tissue Growth Factor (CTGF) ELISA Kit |
abx573322-96tests |
Abbexa |
96 tests |
EUR 825 |
|
Pig Connective Tissue Growth Factor (CTGF) CLIA Kit |
20-abx190822 |
Abbexa |
-
EUR 9242.00
-
EUR 4920.00
-
EUR 1130.00
|
- 10 × 96 tests
- 5 × 96 tests
- 96 tests
|
|
Pig Connective Tissue Growth Factor (CTGF) ELISA Kit |
20-abx154906 |
Abbexa |
-
EUR 7645.00
-
EUR 4074.00
-
EUR 942.00
|
- 10 × 96 tests
- 5 × 96 tests
- 96 tests
|
|
Connective Tissue Growth Factor (CTGF) Polyclonal Antibody (Pig) |
4-PAA010Po01 |
Cloud-Clone |
-
EUR 259.00
-
EUR 2694.00
-
EUR 667.00
-
EUR 326.00
-
EUR 218.00
|
- 100ul
- 10ml
- 1ml
- 200ul
- 20ul
|
|
Description: A Rabbit polyclonal antibody against Pig Connective Tissue Growth Factor (CTGF) |
Pig Connective Tissue Growth Factor (CTGF) ELISA Kit |
SEA010Po-10x96wellstestplate |
Cloud-Clone |
10x96-wells test plate |
EUR 5098.02 |
|
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Pig Connective Tissue Growth Factor (CTGF) in serum, plasma, tissue homogenates and other biological fluids. |
Pig Connective Tissue Growth Factor (CTGF) ELISA Kit |
SEA010Po-1x48wellstestplate |
Cloud-Clone |
1x48-wells test plate |
EUR 507.48 |
|
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Pig Connective Tissue Growth Factor (CTGF) in serum, plasma, tissue homogenates and other biological fluids. |
Pig Connective Tissue Growth Factor (CTGF) ELISA Kit |
SEA010Po-1x96wellstestplate |
Cloud-Clone |
1x96-wells test plate |
EUR 682.12 |
|
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Pig Connective Tissue Growth Factor (CTGF) in serum, plasma, tissue homogenates and other biological fluids. |
Pig Connective Tissue Growth Factor (CTGF) ELISA Kit |
SEA010Po-5x96wellstestplate |
Cloud-Clone |
5x96-wells test plate |
EUR 2769.54 |
|
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Pig Connective Tissue Growth Factor (CTGF) in serum, plasma, tissue homogenates and other biological fluids. |
Pig Connective Tissue Growth Factor (CTGF) ELISA Kit |
4-SEA010Po |
Cloud-Clone |
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EUR 5149.00
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EUR 2720.00
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EUR 683.00
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- 10 plates of 96 wells
- 5 plates of 96 wells
- 1 plate of 96 wells
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Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Pig Connective Tissue Growth Factor (CTGF) in samples from Serum, plasma, tissue homogenates and other biological fluids. with no significant corss-reactivity with analogues from other species. |
Pig Connective Tissue Growth Factor (CTGF)CLIA Kit |
SCA010Po-10x96wellstestplate |
Cloud-Clone |
10x96-wells test plate |
EUR 6747.36 |
|
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Pig Connective Tissue Growth Factor (CTGF) in serum, plasma and other biological fluids. |
Pig Connective Tissue Growth Factor (CTGF)CLIA Kit |
SCA010Po-1x48wellstestplate |
Cloud-Clone |
1x48-wells test plate |
EUR 643.31 |
|
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Pig Connective Tissue Growth Factor (CTGF) in serum, plasma and other biological fluids. |
Mecasermin protein saved stability at 4°C for as much as 2 years. The quantitative and qualitative management indicated that Mecasermin is expressed appropriately (with out the preliminary methionine by Mass spectrometry), pure (with out endotoxin and different protein impurities), appropriate folding (FTIR, RF-HPLC), monomer type (SEC-HPLC) and lively (bioactivity check). Additionally, the purification outcomes revealed that expression in low temperature outcomes the environment friendly purification of the over-produced Mecasermin with a excessive amount and high quality.
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