High-Yield Production of Active Recombinant S. simulans Lysostaphin Expressed in E. coli in a Laboratory Bioreactor

Staphylococcus aureus (S. aureus), which has developed multidrug resistance, results in many healthcare-associated infections leading to vital medical and financial losses. Due to this fact, the event of latest environment friendly methods to cope with these micro organism has been gaining significance. Lysostaphin is a peptidoglycan hydrolase that has appreciable potential as a bacteriocin.

Nonetheless, there have been few reported optimization and scale-up research of the lysostaphin bioproduction course of. Our preliminary outcomes have revealed that the composition of auto-induction media at 30 °C will increase the produced lysostaphin round 10-fold in shake flasks. On this examine, reaching greater yields for recombinant lysostaphin in E. coli at a laboratory scale has been the goal, via using auto-induction media.

Optimized medium composition and fermentation parameters have been transferred to a laboratory-scale bioreactor. The examined circumstances improved protein yields as much as 184 mg/L in a Three L stirred bioreactor and the productiveness was improved 2-fold compared to beforehand printed stories. Moreover, this examine additionally confirmed that lysostaphin is an efficient bacteriocin on each commercially accessible and remoted S. aureus strains. These outcomes will contribute to future larger-scale manufacturing of lysostaphin through the proposed fermentation circumstances.

Combinatorial ethanol remedy will increase the general productiveness of recombinant hG-CSF in E. coli: a comparative examine

Human granulocyte colony-stimulating issue (hG-CSF) is a cytokine that regulates the proliferation, maturation, and differentiation of precursor cells to neutrophils. Within the current examine, we report the feasibility of inducing recombinant hG-CSF expression (rhG-CSF) in a pET vector system by combinatorial induction utilizing low-concentration ethanol, IPTG, and lactose and auto-induction media (AIM). The coding sequence of hG-CSF transcript variant 2 was expressed in pET14 vector, and the impact of combinatorial induction was analyzed on inclusion physique (IB) formation, biomass, protein purification, and bioactivity.

Outcomes confirmed that there was an inverse relationship between the temperature and soluble expression of rhG-CSF. Three-step washing with Triton-X, 2 M, and 5 M urea resulted within the most restoration of IBs. Combinatorial single-spike induction with IPTG, ethanol, and lactose in a batch tradition led to a 3-fold enhance within the expression of rhG-CSF. It was additionally noticed that low focus of ethanol (1-3% v/v) may very well be utilized in lieu of IPTG for inducing the rhG-CSF protein expression with out adversely affecting biomass manufacturing. A 2.4-fold enhance in productiveness was obtained in LB-AIM media with combinatorial ethanol induction, and the general yield of two.eight g/L rhG-CSF was discovered.

The purified rhG-CSF was bioactive and elevated the mobile proliferation of umbilical wire blood-derived mesenchymal stem cells (U-MSC) by 29%. In conclusion, our examine reveals that mixed ethanol induction can improve the expression of rhG-CSF with three-step washing for restoration of the proteins from IBs and a single-step purification of rhG-CSF by affinity chromatography. KEY POINTS: • Low focus of ethanol (1-3%) may very well be utilized in lieu of IPTG for inducing rhG-CSF expression. • Combinatorial single-spike induction with IPTG, ethanol, and lactose improved rhG-CSF expression. • Purified rhG-CSF was bioactive and elevated the proliferation of U-MSC.

Inhibition of E. coli host RNA Polymerase Permits Efficient Extracellular Recombinant Protein Manufacturing by Enhancing Outer Membrane Leakiness

Recombinant proteins in Escherichia coli are often expressed contained in the cell. With the rising curiosity in steady cultivation, secretion of product to the medium just isn’t solely a profit however a necessity in future bioprocessing. On this examine, we present that induced decoupling of development and heterologous gene expression within the E. coli X-press pressure, a pressure derived from BL21(DE3), facilitates extracellular recombinant protein manufacturing.

We investigated the impact of the method parameters temperature and particular glucose consumption price (q_S ) on development, productiveness, lysis and leakiness, to seek out the parameter area permitting extracellular protein manufacturing. Two mannequin proteins have been used, Protein A and a VHH single-domain antibody, and efficiency was in comparison with the economic commonplace pressure BL21(DE3).

We present that inducible development repression within the X-press pressure tremendously mitigates the impact of metabolic burden underneath completely different course of circumstances. Moreover, temperature and q_S have been used to regulate productiveness and leakiness. Within the X-press pressure, extracellular Protein A and VHH titer reached as much as 349 mg/g and 19.6 mg/g, respectively, comprising as much as 90% of the entire soluble product, whereas retaining cell lysis at a minimal.

Recombinant manufacturing of energetic microbial transglutaminase in E. coli by utilizing self-cleavable zymogen with mutated propeptide

Microbial transglutaminase from Streptomyces mobaraensis (MTG) has been broadly utilized in meals business and likewise in analysis and medical functions, since it could site-specifically modify proteins by the cross-linking response of glutamine residue and the first amino group. The recombinant expression system of MTG in E. coli offers higher accessibility for the researchers and thus can promote additional utilization of MTG.

Herein, we report manufacturing of energetic and soluble MTG in E. coli by utilizing a chimeric protein of tobacco etch virus (TEV) protease and MTG zymogen. A chimera of TEV protease and MTG zymogen with native propeptide resulted in energetic MTG contaminated with cleaved propeptide as a result of sturdy interplay between the propeptide and catalytic area of MTG.

Tissue Factor Pathway Inhibitor (TFPI)
MBS6508350-5x01mg	MyBiosource	5x0.1mg	EUR 3725

Recombinant Mouse Tissue factor pathway inhibitor (Tfpi)
MBS1194710-002mgBaculovirus	MyBiosource	0.02mg(Baculovirus)	EUR 1160

Recombinant Mouse Tissue factor pathway inhibitor (Tfpi)
MBS1194710-002mgEColi	MyBiosource	0.02mg(E-Coli)	EUR 795

Recombinant Mouse Tissue factor pathway inhibitor (Tfpi)
MBS1194710-002mgYeast	MyBiosource	0.02mg(Yeast)	EUR 945

Recombinant Mouse Tissue factor pathway inhibitor (Tfpi)
MBS1194710-01mgEColi	MyBiosource	0.1mg(E-Coli)	EUR 955

Recombinant Mouse Tissue factor pathway inhibitor (Tfpi)
MBS1194710-01mgYeast	MyBiosource	0.1mg(Yeast)	EUR 1110

Mouse Tissue Factor Pathway Inhibitor (TFPI) CLIA Kit
20-abx190450	Abbexa	Ask for price Ask for price Ask for price	10 × 96 tests 5 × 96 tests 96 tests

Mouse Tissue Factor Pathway Inhibitor (TFPI) CLIA Kit
abx195578-96tests	Abbexa	96 tests	EUR 990

Mouse Tissue Factor Pathway Inhibitor (TFPI) CLIA Kit
EKU08868-48T	Biomatik Corporation	48T	EUR 685.72

Mouse Tissue Factor Pathway Inhibitor (TFPI) CLIA Kit
EKU08868-5x96T	Biomatik Corporation	5x96T	EUR 4653.1

Mouse Tissue Factor Pathway Inhibitor (TFPI) CLIA Kit
EKU08868-96T	Biomatik Corporation	96T	EUR 979.6

Mouse Tissue Factor Pathway Inhibitor (TFPI) CLIA Kit
EKN49979-48T	Biomatik Corporation	48T	EUR 426.72

Mouse Tissue Factor Pathway Inhibitor (TFPI) CLIA Kit
EKN49979-5x96T	Biomatik Corporation	5x96T	EUR 2895.6

Mouse Tissue Factor Pathway Inhibitor (TFPI) CLIA Kit
EKN49979-96T	Biomatik Corporation	96T	EUR 609.6

Mouse Tissue Factor Pathway Inhibitor (TFPI)CLIA Kit
SCA394Mu-10x96wellstestplate	Cloud-Clone	10x96-wells test plate	EUR 6965.86
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Mouse Tissue Factor Pathway Inhibitor (TFPI) in Plasma, urine, cell culture supernates and other biological fluids.

Mouse Tissue Factor Pathway Inhibitor (TFPI)CLIA Kit
SCA394Mu-1x48wellstestplate	Cloud-Clone	1x48-wells test plate	EUR 678.84
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Mouse Tissue Factor Pathway Inhibitor (TFPI) in Plasma, urine, cell culture supernates and other biological fluids.

Mouse Tissue Factor Pathway Inhibitor (TFPI)CLIA Kit
SCA394Mu-1x96wellstestplate	Cloud-Clone	1x96-wells test plate	EUR 918.34
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Mouse Tissue Factor Pathway Inhibitor (TFPI) in Plasma, urine, cell culture supernates and other biological fluids.

Mouse Tissue Factor Pathway Inhibitor (TFPI)CLIA Kit
SCA394Mu-5x96wellstestplate	Cloud-Clone	5x96-wells test plate	EUR 3772.51
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Mouse Tissue Factor Pathway Inhibitor (TFPI) in Plasma, urine, cell culture supernates and other biological fluids.

Mouse Tissue Factor Pathway Inhibitor (TFPI) CLIA Kit
4-SCA394Mu	Cloud-Clone	Ask for price Ask for price Ask for price	10 plates of 96 wells 5 plates of 96 wells 1 plate of 96 wells
Description: Double-antibody Sandwich chemiluminescent immunoassay for detection of Mouse Tissue Factor Pathway Inhibitor (TFPI)Plasma, urine, cell culture supernates and other biological fluids

Mouse Tissue Factor Pathway Inhibitor (TFPI) CLIA Kit
MBS2025453-10x96StripWells	MyBiosource	10x96-Strip-Wells	EUR 6315

Mouse Tissue Factor Pathway Inhibitor (TFPI) CLIA Kit
MBS2025453-24StripWells	MyBiosource	24-Strip-Wells	EUR 415

Mouse Tissue Factor Pathway Inhibitor (TFPI) CLIA Kit
MBS2025453-48StripWells	MyBiosource	48-Strip-Wells	EUR 640

Mouse Tissue Factor Pathway Inhibitor (TFPI) CLIA Kit
MBS2025453-5x96StripWells	MyBiosource	5x96-Strip-Wells	EUR 3480

Mouse Tissue Factor Pathway Inhibitor (TFPI) CLIA Kit
MBS2025453-96StripWells	MyBiosource	96-Strip-Wells	EUR 865

Mouse Tissue Factor Pathway Inhibitor (TFPI) CLIA Kit
MBS2708756-10x96StripWells	MyBiosource	10x96-Strip-Wells	EUR 3840

Mouse Tissue Factor Pathway Inhibitor (TFPI) CLIA Kit
MBS2708756-24StripWells	MyBiosource	24-Strip-Wells	EUR 280

Mouse Tissue Factor Pathway Inhibitor (TFPI) CLIA Kit
MBS2708756-48StripWells	MyBiosource	48-Strip-Wells	EUR 375

Mouse Tissue Factor Pathway Inhibitor (TFPI) CLIA Kit
MBS2708756-5x96StripWells	MyBiosource	5x96-Strip-Wells	EUR 1985

Mouse Tissue Factor Pathway Inhibitor (TFPI) CLIA Kit
MBS2708756-96StripWells	MyBiosource	96-Strip-Wells	EUR 490

Mouse Tissue Factor Pathway Inhibitor (TFPI) ELISA Kit
20-abx054160	Abbexa	Ask for price Ask for price Ask for price	10 × 96 tests 5 × 96 tests 96 tests

Mouse Tissue Factor Pathway Inhibitor (TFPI) ELISA Kit
abx254457-96tests	Abbexa	96 tests	EUR 801.6

Mouse Tissue Factor Pathway Inhibitor (TFPI) ELISA Kit
abx576269-96tests	Abbexa	96 tests	EUR 801.6

Mouse Tissue Factor Pathway Inhibitor (TFPI) ELISA Kit
20-abx154740	Abbexa	Ask for price Ask for price Ask for price	10 × 96 tests 5 × 96 tests 96 tests

Mouse Tissue Factor Pathway Inhibitor (TFPI) ELISA Kit
DLR-TFPI-Mu	DL Develop	96T	EUR 432
Description: plasma, urine, cell culture supernates or other biological fluids.

Mouse Tissue Factor Pathway Inhibitor (TFPI) ELISA Kit
DLR-TFPI-Mu-48T	DL Develop	48T	EUR 586.8
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Tissue Factor Pathway Inhibitor (TFPI) in samples from plasma, urine, cell culture supernates or other biological fluids.

Mouse Tissue Factor Pathway Inhibitor (TFPI) ELISA Kit
DLR-TFPI-Mu-96T	DL Develop	96T	EUR 762
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Tissue Factor Pathway Inhibitor (TFPI) in samples from plasma, urine, cell culture supernates or other biological fluids.

Mouse Tissue Factor Pathway Inhibitor (TFPI) ELISA Kit
DL-TFPI-Mu	DL Develop	96T	EUR 412
Description: plasma, urine, cell culture supernates or other biological fluids.

Mouse Tissue factor pathway inhibitor,TFPI Elisa Kit
EK730343	AFG Bioscience LLC	96 Wells	EUR 0.67

Mouse Tissue Factor Pathway Inhibitor (TFPI) ELISA Kit
EKN48813-48T	Biomatik Corporation	48T	EUR 350.42

Mouse Tissue Factor Pathway Inhibitor (TFPI) ELISA Kit
EKN48813-5x96T	Biomatik Corporation	5x96T	EUR 2377.85

Mouse Tissue Factor Pathway Inhibitor (TFPI) ELISA Kit
EKN48813-96T	Biomatik Corporation	96T	EUR 500.6

Mouse Tissue Factor Pathway Inhibitor (TFPI) ELISA Kit
EK16847	SAB	96Т	EUR 768

Mouse Tissue factor pathway inhibitor, TFPI ELISA Kit
CSB-E08262m-24T	Cusabio	1 plate of 24 wells	EUR 198
Description: Quantitativesandwich ELISA kit for measuring Mouse Tissue factor pathway inhibitor, TFPI in samples from serum, plasma, cell culture supernates, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Mouse Tissue factor pathway inhibitor, TFPI ELISA Kit
1-CSB-E08262m	Cusabio	Ask for price Ask for price Ask for price	1 plate of 96 wells 10 plates of 96 wells each 5 plates of 96 wells each
Description: Quantitativesandwich ELISA kit for measuring Mouse Tissue factor pathway inhibitor, TFPI in samples from serum, plasma, cell culture supernates, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Mouse Tissue factor pathway inhibitor,TFPI ELISA Kit
CN-02305M1	ChemNorm	96T	EUR 558

Mouse Tissue factor pathway inhibitor,TFPI ELISA Kit
CN-02305M2	ChemNorm	48T	EUR 378

Mouse Tissue Factor Pathway Inhibitor (TFPI) ELISA Kit
SEA394Mu-10x96wellstestplate	Cloud-Clone	10x96-wells test plate	EUR 5269.39
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Tissue Factor Pathway Inhibitor (TFPI) in Plasma, urine, cell culture supernates and other biological fluids.

Mouse Tissue Factor Pathway Inhibitor (TFPI) ELISA Kit
SEA394Mu-1x48wellstestplate	Cloud-Clone	1x48-wells test plate	EUR 539.12
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Tissue Factor Pathway Inhibitor (TFPI) in Plasma, urine, cell culture supernates and other biological fluids.

Mouse Tissue Factor Pathway Inhibitor (TFPI) ELISA Kit
SEA394Mu-1x96wellstestplate	Cloud-Clone	1x96-wells test plate	EUR 718.75
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Tissue Factor Pathway Inhibitor (TFPI) in Plasma, urine, cell culture supernates and other biological fluids.

Mouse Tissue Factor Pathway Inhibitor (TFPI) ELISA Kit
SEA394Mu-5x96wellstestplate	Cloud-Clone	5x96-wells test plate	EUR 2874.38
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Tissue Factor Pathway Inhibitor (TFPI) in Plasma, urine, cell culture supernates and other biological fluids.

Mouse Tissue Factor Pathway Inhibitor (TFPI) ELISA Kit
4-SEA394Mu	Cloud-Clone	Ask for price Ask for price Ask for price	10 plates of 96 wells 5 plates of 96 wells 1 plate of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Mouse Tissue Factor Pathway Inhibitor (TFPI) in samples from Plasma, urine, cell culture supernates and other biological fluids with no significant corss-reactivity with analogues from other species.

Mouse Tissue factor pathway inhibitor,TFPI ELISA Kit
YLA0745MO-48T	Shanghai YL Biotech	48T	Ask for price

Mouse Tissue factor pathway inhibitor,TFPI ELISA Kit
YLA0745MO-96T	Shanghai YL Biotech	96T	Ask for price

Mouse Tissue factor pathway inhibitor (TFPI) ELISA Kit
AE17137MO-48T	Abebio	48T	EUR 325
Description: Mouse (Mus musculus)

Mouse Tissue factor pathway inhibitor (TFPI) ELISA Kit
AE17137MO-96T	Abebio	96T	EUR 680
Description: Mouse (Mus musculus)

Mouse Tissue factor pathway inhibitor(TFPI)ELISA Kit
GA-E0281MS-48Tests	GenAsia Biotech	48 Tests	Ask for price

Mouse Tissue factor pathway inhibitor(TFPI)ELISA Kit
GA-E0281MS-96Tests	GenAsia Biotech	96 Tests	EUR 460

Mouse Tissue factor pathway inhibitor(TFPI)ELISA Kit
GA-E0281MS-48T	GenAsia Biotech	48T	EUR 403.2

Mouse Tissue factor pathway inhibitor(TFPI)ELISA Kit
GA-E0281MS-96T	GenAsia Biotech	96T	EUR 640.8

Mouse Tissue Factor Pathway Inhibitor (TFPI) ELISA Kit
EKU07702-48T	Biomatik Corporation	48T	EUR 514.08

Mouse Tissue Factor Pathway Inhibitor (TFPI) ELISA Kit
EKU07702-5x96T	Biomatik Corporation	5x96T	EUR 3488.4

Mouse Tissue Factor Pathway Inhibitor (TFPI) ELISA Kit
EKU07702-96T	Biomatik Corporation	96T	EUR 734.4

Mouse Tissue Factor Pathway Inhibitor (TFPI) ELISA Kit
IMSTFPIKT	Innovative research	each	EUR 702
Description: Mouse Tissue Factor Pathway Inhibitor (TFPI) ELISA Kit

Mouse Tissue factor pathway inhibitor(TFPI)ELISA Kit
QY-E21471	Qayee Biotechnology	96T	EUR 433.2

Mouse Tissue factor pathway inhibitor (TFPI) ELISA Kit
RK06726	Abclonal	96T	EUR 280

Mouse Tissue Factor Pathway Inhibitor (TFPI) ELISA Kit
RDR-TFPI-Mu-48T	Reddot Biotech	48T	EUR 443.42
Description: plasma, urine, cell culture supernatants and other biological fluids.

Mouse Tissue Factor Pathway Inhibitor (TFPI) ELISA Kit
RDR-TFPI-Mu-48Tests	Reddot Biotech	48 Tests	EUR 613.2

Mouse Tissue Factor Pathway Inhibitor (TFPI) ELISA Kit
RDR-TFPI-Mu-96T	Reddot Biotech	96T	EUR 633.47
Description: plasma, urine, cell culture supernatants and other biological fluids.

Mouse Tissue Factor Pathway Inhibitor (TFPI) ELISA Kit
RDR-TFPI-Mu-96Tests	Reddot Biotech	96 Tests	EUR 850.8

Mouse Tissue Factor Pathway Inhibitor (TFPI) ELISA Kit
RD-TFPI-Mu-48T	Reddot Biotech	48T	EUR 422.3
Description: plasma, urine, cell culture supernatants and other biological fluids.

Mouse Tissue Factor Pathway Inhibitor (TFPI) ELISA Kit
RD-TFPI-Mu-48Tests	Reddot Biotech	48 Tests	EUR 586.8

Mouse Tissue Factor Pathway Inhibitor (TFPI) ELISA Kit
RD-TFPI-Mu-96T	Reddot Biotech	96T	EUR 603.3
Description: plasma, urine, cell culture supernatants and other biological fluids.

Mouse Tissue Factor Pathway Inhibitor (TFPI) ELISA Kit
RD-TFPI-Mu-96Tests	Reddot Biotech	96 Tests	EUR 812.4

Mouse Tissue factor pathway inhibitor,TFPI ELISA Kit
SL0531Mo	Sunlong	-	EUR 528

Mouse Tissue Factor Pathway Inhibitor (TFPI) ELISA Kit
abx054160-100g	Abbexa	100 µg	EUR 2950

Mouse Tissue Factor Pathway Inhibitor (TFPI) ELISA Kit
abx054160-200g	Abbexa	200 µg	EUR 5575

Mouse Tissue Factor Pathway Inhibitor (TFPI) ELISA Kit
abx054160-50g	Abbexa	50 µg	EUR 618.75

Mouse Tissue factor pathway inhibitor,TFPI ELISA KIT
E0268Mo-1096T	Jiaxing Korain Biotech Ltd (BT Labs)	10*96T	EUR 4122

Mouse Tissue factor pathway inhibitor,TFPI ELISA KIT
E0268Mo-48wells	Jiaxing Korain Biotech Ltd (BT Labs)	48 wells	EUR 300

Mouse Tissue factor pathway inhibitor,TFPI ELISA KIT
E0268Mo-596T	Jiaxing Korain Biotech Ltd (BT Labs)	5*96T	EUR 2061

Mouse Tissue factor pathway inhibitor,TFPI ELISA KIT
E0268Mo-96wells	Jiaxing Korain Biotech Ltd (BT Labs)	96 wells	EUR 458

Mouse Tissue Factor Pathway Inhibitor (TFPI) ELISA Kit
MBS8420894-1Kit	MyBiosource	1Kit	EUR 755

Mouse Tissue Factor Pathway Inhibitor (TFPI) ELISA Kit
MBS8420894-5x1Kit	MyBiosource	5x1Kit	EUR 3455

Mouse Tissue factor pathway inhibitor, TFPI ELISA Kit
MBS162794-10x96StripWells	MyBiosource	10x96-Strip-Wells	EUR 3955

Mouse Tissue factor pathway inhibitor, TFPI ELISA Kit
MBS162794-48StripWells	MyBiosource	48-Strip-Wells	EUR 305

Mouse Tissue factor pathway inhibitor, TFPI ELISA Kit
MBS162794-5x96StripWells	MyBiosource	5x96-Strip-Wells	EUR 2005

Mouse Tissue factor pathway inhibitor, TFPI ELISA Kit
MBS162794-96StripWells	MyBiosource	96-Strip-Wells	EUR 475

Mouse Tissue Factor Pathway Inhibitor (TFPI) ELISA Kit
MBS2023841-10x96StripWells	MyBiosource	10x96-Strip-Wells	EUR 4995

Mouse Tissue Factor Pathway Inhibitor (TFPI) ELISA Kit
MBS2023841-24StripWells	MyBiosource	24-Strip-Wells	EUR 345

Mouse Tissue Factor Pathway Inhibitor (TFPI) ELISA Kit
MBS2023841-48StripWells	MyBiosource	48-Strip-Wells	EUR 510

Mouse Tissue Factor Pathway Inhibitor (TFPI) ELISA Kit
MBS2023841-5x96StripWells	MyBiosource	5x96-Strip-Wells	EUR 2735

Mouse Tissue Factor Pathway Inhibitor (TFPI) ELISA Kit
MBS2023841-96StripWells	MyBiosource	96-Strip-Wells	EUR 680

Mouse Tissue Factor Pathway Inhibitor (TFPI) ELISA Kit
MBS4502788-10x96Tests	MyBiosource	10x96Tests	EUR 4965

Mouse Tissue Factor Pathway Inhibitor (TFPI) ELISA Kit
MBS4502788-48Tests	MyBiosource	48Tests	EUR 435

Mouse Tissue Factor Pathway Inhibitor (TFPI) ELISA Kit
MBS4502788-5x96Tests	MyBiosource	5x96Tests	EUR 2550

Mouse Tissue Factor Pathway Inhibitor (TFPI) ELISA Kit
MBS4502788-96Test	MyBiosource	96Test	EUR 575

Mouse Tissue Factor Pathway Inhibitor (TFPI) ELISA Kit
MBS2701737-10x96StripWells	MyBiosource	10x96-Strip-Wells	EUR 2905

Mouse Tissue Factor Pathway Inhibitor (TFPI) ELISA Kit
MBS2701737-24StripWells	MyBiosource	24-Strip-Wells	EUR 240

Mouse Tissue Factor Pathway Inhibitor (TFPI) ELISA Kit
MBS2701737-48StripWells	MyBiosource	48-Strip-Wells	EUR 320

Mouse Tissue Factor Pathway Inhibitor (TFPI) ELISA Kit
MBS2701737-5x96StripWells	MyBiosource	5x96-Strip-Wells	EUR 1515

Mouse Tissue Factor Pathway Inhibitor (TFPI) ELISA Kit
MBS2701737-96StripWells	MyBiosource	96-Strip-Wells	EUR 390

Mouse Tissue Factor Pathway Inhibitor (TFPI) ELISA Kit
MBS452924-10x96StripWells	MyBiosource	10x96-Strip-Wells	EUR 4800

Mouse Tissue Factor Pathway Inhibitor (TFPI) ELISA Kit
MBS452924-48StripWells	MyBiosource	48-Strip-Wells	EUR 420

Mouse Tissue Factor Pathway Inhibitor (TFPI) ELISA Kit
MBS452924-5x96StripWells	MyBiosource	5x96-Strip-Wells	EUR 2465

Mouse Tissue Factor Pathway Inhibitor (TFPI) ELISA Kit
MBS452924-96StripWells	MyBiosource	96-Strip-Wells	EUR 550

Mouse Tissue Factor Pathway Inhibitor (TFPI) ELISA Kit
MBS266498-10x96StripWells	MyBiosource	10x96-Strip-Wells	EUR 3290

Mouse Tissue Factor Pathway Inhibitor (TFPI) ELISA Kit
MBS266498-48StripWells	MyBiosource	48-Strip-Wells	EUR 295

Mouse Tissue Factor Pathway Inhibitor (TFPI) ELISA Kit
MBS266498-5x96StripWells	MyBiosource	5x96-Strip-Wells	EUR 1800

Mouse Tissue Factor Pathway Inhibitor (TFPI) ELISA Kit
MBS266498-96StripWells	MyBiosource	96-Strip-Wells	EUR 450

Mouse Tissue Factor Pathway Inhibitor (TFPI)ELISA Kit
EIA06424m	Enlibio	each	EUR 480

Mouse Tissue Factor pathway Inhibitor, TFPI GENLISA ELISA
KLM0268	Krishgen	1 x 96 wells	EUR 341

Introduction of mutations of K9R and Y11A to the propeptide facilitated dissociation of the cleaved propeptide from the catalytic area of MTG and energetic MTG with none contamination of the propeptide was obtained. The precise exercise of the energetic MTG was 22.7±2.6 U/mg. The profitable expression and purification of energetic MTG by utilizing the chimera protein of TEV protease and MTG zymogen with mutations within the propeptide can advance using MTG and the researches utilizing MTG mediated cross-linking reactions.