High-Yield Production of Active Recombinant S. simulans Lysostaphin Expressed in E. coli in a Laboratory Bioreactor

Staphylococcus aureus (S. aureus), which has developed multidrug resistance, results in many healthcare-associated infections leading to vital medical and financial losses. Due to this fact, the event of latest environment friendly methods to cope with these micro organism has been gaining significance. Lysostaphin is a peptidoglycan hydrolase that has appreciable potential as a bacteriocin.

Nonetheless, there have been few reported optimization and scale-up research of the lysostaphin bioproduction course of. Our preliminary outcomes have revealed that the composition of auto-induction media at 30 °C will increase the produced lysostaphin round 10-fold in shake flasks. On this examine, reaching greater yields for recombinant lysostaphin in E. coli at a laboratory scale has been the goal, via using auto-induction media.

Optimized medium composition and fermentation parameters have been transferred to a laboratory-scale bioreactor. The examined circumstances improved protein yields as much as 184 mg/L in a Three L stirred bioreactor and the productiveness was improved 2-fold compared to beforehand printed stories. Moreover, this examine additionally confirmed that lysostaphin is an efficient bacteriocin on each commercially accessible and remoted S. aureus strains. These outcomes will contribute to future larger-scale manufacturing of lysostaphin through the proposed fermentation circumstances.

Combinatorial ethanol remedy will increase the general productiveness of recombinant hG-CSF in Ecoli: a comparative examine

Human granulocyte colony-stimulating issue (hG-CSF) is a cytokine that regulates the proliferation, maturation, and differentiation of precursor cells to neutrophils. Within the current examine, we report the feasibility of inducing recombinant hG-CSF expression (rhG-CSF) in a pET vector system by combinatorial induction utilizing low-concentration ethanol, IPTG, and lactose and auto-induction media (AIM). The coding sequence of hG-CSF transcript variant 2 was expressed in pET14 vector, and the impact of combinatorial induction was analyzed on inclusion physique (IB) formation, biomass, protein purification, and bioactivity.

Outcomes confirmed that there was an inverse relationship between the temperature and soluble expression of rhG-CSF. Three-step washing with Triton-X, 2 M, and 5 M urea resulted within the most restoration of IBs. Combinatorial single-spike induction with IPTG, ethanol, and lactose in a batch tradition led to a 3-fold enhance within the expression of rhG-CSF. It was additionally noticed that low focus of ethanol (1-3% v/v) may very well be utilized in lieu of IPTG for inducing the rhG-CSF protein expression with out adversely affecting biomass manufacturing. A 2.4-fold enhance in productiveness was obtained in LB-AIM media with combinatorial ethanol induction, and the general yield of two.eight g/L rhG-CSF was discovered.

The purified rhG-CSF was bioactive and elevated the mobile proliferation of umbilical wire blood-derived mesenchymal stem cells (U-MSC) by 29%. In conclusion, our examine reveals that mixed ethanol induction can improve the expression of rhG-CSF with three-step washing for restoration of the proteins from IBs and a single-step purification of rhG-CSF by affinity chromatography. KEY POINTS: • Low focus of ethanol (1-3%) may very well be utilized in lieu of IPTG for inducing rhG-CSF expression. • Combinatorial single-spike induction with IPTG, ethanol, and lactose improved rhG-CSF expression. • Purified rhG-CSF was bioactive and elevated the proliferation of U-MSC.

Inhibition of Ecoli host RNA Polymerase Permits Efficient Extracellular Recombinant Protein Manufacturing by Enhancing Outer Membrane Leakiness

Recombinant proteins in Escherichia coli are often expressed contained in the cell. With the rising curiosity in steady cultivation, secretion of product to the medium just isn’t solely a profit however a necessity in future bioprocessing. On this examine, we present that induced decoupling of development and heterologous gene expression within the E. coli X-press pressure, a pressure derived from BL21(DE3), facilitates extracellular recombinant protein manufacturing.

We investigated the impact of the method parameters temperature and particular glucose consumption price (qS ) on development, productiveness, lysis and leakiness, to seek out the parameter area permitting extracellular protein manufacturing. Two mannequin proteins have been used, Protein A and a VHH single-domain antibody, and efficiency was in comparison with the economic commonplace pressure BL21(DE3).

We present that inducible development repression within the X-press pressure tremendously mitigates the impact of metabolic burden underneath completely different course of circumstances. Moreover, temperature and qS have been used to regulate productiveness and leakiness. Within the X-press pressure, extracellular Protein A and VHH titer reached as much as 349 mg/g and 19.6 mg/g, respectively, comprising as much as 90% of the entire soluble product, whereas retaining cell lysis at a minimal.

High-Yield Production of Active Recombinant S. simulans Lysostaphin Expressed in E. coli in a Laboratory Bioreactor

Recombinant manufacturing of energetic microbial transglutaminase in Ecoli by utilizing self-cleavable zymogen with mutated propeptide

Microbial transglutaminase from Streptomyces mobaraensis (MTG) has been broadly utilized in meals business and likewise in analysis and medical functions, since it could site-specifically modify proteins by the cross-linking response of glutamine residue and the first amino group. The recombinant expression system of MTG in E. coli offers higher accessibility for the researchers and thus can promote additional utilization of MTG.

Herein, we report manufacturing of energetic and soluble MTG in E. coli by utilizing a chimeric protein of tobacco etch virus (TEV) protease and MTG zymogen. A chimera of TEV protease and MTG zymogen with native propeptide resulted in energetic MTG contaminated with cleaved propeptide as a result of sturdy interplay between the propeptide and catalytic area of MTG.

Mouse Tissue Factor Pathway Inhibitor (TFPI) CLIA Kit

EKN49979-48T 48T
EUR 426.72

Mouse Tissue Factor Pathway Inhibitor (TFPI) CLIA Kit

EKN49979-5x96T 5x96T
EUR 2895.6

Mouse Tissue Factor Pathway Inhibitor (TFPI) CLIA Kit

EKN49979-96T 96T
EUR 609.6

Mouse Tissue Factor Pathway Inhibitor (TFPI) CLIA Kit

20-abx190450
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Mouse Tissue Factor Pathway Inhibitor (TFPI) CLIA Kit

abx195578-96tests 96 tests
EUR 990

Mouse Tissue Factor Pathway Inhibitor (TFPI)CLIA Kit

SCA394Mu-10x96wellstestplate 10x96-wells test plate
EUR 6965.86
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Mouse Tissue Factor Pathway Inhibitor (TFPI) in Plasma, urine, cell culture supernates and other biological fluids.

Mouse Tissue Factor Pathway Inhibitor (TFPI)CLIA Kit

SCA394Mu-1x48wellstestplate 1x48-wells test plate
EUR 678.84
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Mouse Tissue Factor Pathway Inhibitor (TFPI) in Plasma, urine, cell culture supernates and other biological fluids.

Mouse Tissue Factor Pathway Inhibitor (TFPI)CLIA Kit

SCA394Mu-1x96wellstestplate 1x96-wells test plate
EUR 918.34
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Mouse Tissue Factor Pathway Inhibitor (TFPI) in Plasma, urine, cell culture supernates and other biological fluids.

Mouse Tissue Factor Pathway Inhibitor (TFPI)CLIA Kit

SCA394Mu-5x96wellstestplate 5x96-wells test plate
EUR 3772.51
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Mouse Tissue Factor Pathway Inhibitor (TFPI) in Plasma, urine, cell culture supernates and other biological fluids.

Mouse Tissue Factor Pathway Inhibitor (TFPI) CLIA Kit

4-SCA394Mu
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  • 10 plates of 96 wells
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Description: Double-antibody Sandwich chemiluminescent immunoassay for detection of Mouse Tissue Factor Pathway Inhibitor (TFPI)Plasma, urine, cell culture supernates and other biological fluids

Mouse Tissue Factor Pathway Inhibitor (TFPI) ELISA Kit

DLR-TFPI-Mu 96T
EUR 432
Description: plasma, urine, cell culture supernates or other biological fluids.

Mouse Tissue Factor Pathway Inhibitor (TFPI) ELISA Kit

DLR-TFPI-Mu-48T 48T
EUR 586.8
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Tissue Factor Pathway Inhibitor (TFPI) in samples from plasma, urine, cell culture supernates or other biological fluids.

Mouse Tissue Factor Pathway Inhibitor (TFPI) ELISA Kit

DLR-TFPI-Mu-96T 96T
EUR 762
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Tissue Factor Pathway Inhibitor (TFPI) in samples from plasma, urine, cell culture supernates or other biological fluids.

Mouse Tissue factor pathway inhibitor,TFPI Elisa Kit

EK730343 96 Wells
EUR 0.67

Mouse Tissue Factor Pathway Inhibitor (TFPI) ELISA Kit

EKU07702-48T 48T
EUR 514.08

Mouse Tissue Factor Pathway Inhibitor (TFPI) ELISA Kit

EKU07702-5x96T 5x96T
EUR 3488.4

Mouse Tissue Factor Pathway Inhibitor (TFPI) ELISA Kit

EKU07702-96T 96T
EUR 734.4

Mouse Tissue Factor Pathway Inhibitor (TFPI) ELISA Kit

EKN48813-48T 48T
EUR 350.42

Mouse Tissue Factor Pathway Inhibitor (TFPI) ELISA Kit

EKN48813-5x96T 5x96T
EUR 2377.85

Mouse Tissue Factor Pathway Inhibitor (TFPI) ELISA Kit

EKN48813-96T 96T
EUR 500.6

Mouse Tissue factor pathway inhibitor(TFPI)ELISA Kit

GA-E0281MS-48T 48T
EUR 403.2

Mouse Tissue factor pathway inhibitor(TFPI)ELISA Kit

GA-E0281MS-96T 96T
EUR 640.8

Mouse Tissue Factor Pathway Inhibitor (TFPI) ELISA Kit

DL-TFPI-Mu 96T
EUR 412
Description: plasma, urine, cell culture supernates or other biological fluids.

Mouse Tissue Factor Pathway Inhibitor (TFPI) ELISA Kit

EK16847 96Т
EUR 768

Mouse Tissue Factor Pathway Inhibitor (TFPI) ELISA Kit

abx576269-96tests 96 tests
EUR 801.6

Mouse Tissue factor pathway inhibitor, TFPI ELISA Kit

CSB-E08262m-24T 1 plate of 24 wells
EUR 198
Description: Quantitativesandwich ELISA kit for measuring Mouse Tissue factor pathway inhibitor, TFPI in samples from serum, plasma, cell culture supernates, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Mouse Tissue factor pathway inhibitor, TFPI ELISA Kit

1-CSB-E08262m
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  • 1 plate of 96 wells
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Description: Quantitativesandwich ELISA kit for measuring Mouse Tissue factor pathway inhibitor, TFPI in samples from serum, plasma, cell culture supernates, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Mouse Tissue Factor Pathway Inhibitor (TFPI) ELISA Kit

20-abx054160
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  • 10 × 96 tests
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Mouse Tissue factor pathway inhibitor,TFPI ELISA Kit

CN-02305M1 96T
EUR 558

Mouse Tissue factor pathway inhibitor,TFPI ELISA Kit

CN-02305M2 48T
EUR 378

Mouse Tissue Factor Pathway Inhibitor (TFPI) ELISA Kit

abx254457-96tests 96 tests
EUR 801.6

Mouse Tissue Factor Pathway Inhibitor (TFPI) ELISA Kit

20-abx154740
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  • 10 × 96 tests
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Mouse Tissue Factor Pathway Inhibitor (TFPI) ELISA Kit

RD-TFPI-Mu-48T 48T
EUR 422.3
Description: plasma, urine, cell culture supernatants and other biological fluids.

Mouse Tissue Factor Pathway Inhibitor (TFPI) ELISA Kit

RD-TFPI-Mu-48Tests 48 Tests
EUR 586.8

Mouse Tissue Factor Pathway Inhibitor (TFPI) ELISA Kit

RD-TFPI-Mu-96T 96T
EUR 603.3
Description: plasma, urine, cell culture supernatants and other biological fluids.

Mouse Tissue Factor Pathway Inhibitor (TFPI) ELISA Kit

RD-TFPI-Mu-96Tests 96 Tests
EUR 812.4

Mouse Tissue factor pathway inhibitor (TFPI) ELISA Kit

RK06726 96T
EUR 280

Mouse Tissue Factor Pathway Inhibitor (TFPI) ELISA Kit

IMSTFPIKT each
EUR 702
Description: Mouse Tissue Factor Pathway Inhibitor (TFPI) ELISA Kit

Mouse Tissue factor pathway inhibitor(TFPI)ELISA Kit

QY-E21471 96T
EUR 433.2

Mouse Tissue Factor Pathway Inhibitor (TFPI) ELISA Kit

SEA394Mu-10x96wellstestplate 10x96-wells test plate
EUR 5269.39
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Tissue Factor Pathway Inhibitor (TFPI) in Plasma, urine, cell culture supernates and other biological fluids.

Mouse Tissue Factor Pathway Inhibitor (TFPI) ELISA Kit

SEA394Mu-1x48wellstestplate 1x48-wells test plate
EUR 539.12
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Tissue Factor Pathway Inhibitor (TFPI) in Plasma, urine, cell culture supernates and other biological fluids.

Mouse Tissue Factor Pathway Inhibitor (TFPI) ELISA Kit

SEA394Mu-1x96wellstestplate 1x96-wells test plate
EUR 718.75
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Tissue Factor Pathway Inhibitor (TFPI) in Plasma, urine, cell culture supernates and other biological fluids.

Mouse Tissue Factor Pathway Inhibitor (TFPI) ELISA Kit

SEA394Mu-5x96wellstestplate 5x96-wells test plate
EUR 2874.38
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Tissue Factor Pathway Inhibitor (TFPI) in Plasma, urine, cell culture supernates and other biological fluids.

Mouse Tissue Factor Pathway Inhibitor (TFPI) ELISA Kit

4-SEA394Mu
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  • 10 plates of 96 wells
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Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Mouse Tissue Factor Pathway Inhibitor (TFPI) in samples from Plasma, urine, cell culture supernates and other biological fluids with no significant corss-reactivity with analogues from other species.

Mouse Tissue factor pathway inhibitor,TFPI ELISA Kit

SL0531Mo -
EUR 528

Mouse Tissue Factor Pathway Inhibitor (TFPI) ELISA Kit

RDR-TFPI-Mu-48T 48T
EUR 443.42
Description: plasma, urine, cell culture supernatants and other biological fluids.

Mouse Tissue Factor Pathway Inhibitor (TFPI) ELISA Kit

RDR-TFPI-Mu-48Tests 48 Tests
EUR 613.2

Mouse Tissue Factor Pathway Inhibitor (TFPI) ELISA Kit

RDR-TFPI-Mu-96T 96T
EUR 633.47
Description: plasma, urine, cell culture supernatants and other biological fluids.

Mouse Tissue Factor Pathway Inhibitor (TFPI) ELISA Kit

RDR-TFPI-Mu-96Tests 96 Tests
EUR 850.8

Mouse Tissue factor pathway inhibitor,TFPI ELISA Kit

YLA0745MO-48T 48T Ask for price

Mouse Tissue factor pathway inhibitor,TFPI ELISA Kit

YLA0745MO-96T 96T Ask for price

Mouse Tissue Factor Pathway Inhibitor (TFPI) ELISA Kit

abx054160-100g 100 µg
EUR 2950

Mouse Tissue Factor Pathway Inhibitor (TFPI) ELISA Kit

abx054160-200g 200 µg
EUR 5575

Mouse Tissue Factor Pathway Inhibitor (TFPI) ELISA Kit

abx054160-50g 50 µg
EUR 618.75

Human Tissue factor pathway inhibitor (TFPI)

1-CSB-EP023437HU
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  • 100ug
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Description: Recombinant Human Tissue factor pathway inhibitor(TFPI),partial expressed in E.coli

Rabbit Tissue factor pathway inhibitor (TFPI)

1-CSB-YP023437RB
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  • 100ug
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Description: Recombinant Rabbit Tissue factor pathway inhibitor(TFPI) expressed in Yeast

Rabbit Tissue factor pathway inhibitor (TFPI)

1-CSB-EP023437RB
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  • 100ug
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  • 1MG
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Description: Recombinant Rabbit Tissue factor pathway inhibitor(TFPI) expressed in E.coli

Rabbit Tissue factor pathway inhibitor (TFPI)

1-CSB-MP023437RB
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  • 100ug
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Description: Recombinant Rabbit Tissue factor pathway inhibitor(TFPI) expressed in Mammalian cell

Active Tissue Factor Pathway Inhibitor (TFPI)

APA394Hu61 10ug
EUR 360

Rabbit Tissue factor pathway inhibitor (TFPI)

1-CSB-BP023437RB
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  • 100ug
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Description: Recombinant Rabbit Tissue factor pathway inhibitor(TFPI) expressed in Baculovirus

Mouse Tissue Factor pathway Inhibitor, TFPI GENLISA ELISA

KLM0268 1 x 96 wells
EUR 341

Tissue Factor Pathway Inhibitor (TFPI) Protein

20-abx261170
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  • 1 mg
  • 20 ug
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Tissue Factor Pathway Inhibitor (TFPI) Protein

abx261170-10g 10 µg
EUR 325

Tissue Factor Pathway Inhibitor (TFPI) Protein

abx261170-2g 2 µg
EUR 225

Mouse TFPI -Tissue Factor Pathway Inhibitor- CLIA Kit

E-CL-M0650-24Tests 24 Tests
EUR 180
Description: Sandwich

Mouse TFPI -Tissue Factor Pathway Inhibitor- CLIA Kit

E-CL-M0650-48Tests 48 Tests
EUR 546
Description: Sandwich

Mouse TFPI -Tissue Factor Pathway Inhibitor- CLIA Kit

E-CL-M0650-96Tests 96 Tests
EUR 682
Description: Sandwich

Mouse TFPI -Tissue Factor Pathway Inhibitor- CLIA Kit

E-CL-M0650-96Tests10 96 Tests *10
EUR 6820
Description: Sandwich

Mouse TFPI -Tissue Factor Pathway Inhibitor- CLIA Kit

E-CL-M0650-96Tests5 96 Tests *5
EUR 3410
Description: Sandwich

Tissue Factor Pathway Inhibitor (TFPI) Antibody

20-abx323593
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  • 100 ug
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Tissue Factor Pathway Inhibitor (TFPI) Antibody

abx433382-200ul 200 ul
EUR 460.8

Tissue Factor Pathway Inhibitor (TFPI) Antibody

20-abx129517
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  • 100 ug
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Tissue Factor Pathway Inhibitor (TFPI) Antibody

20-abx132084
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  • 100 ug
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Tissue Factor Pathway Inhibitor (TFPI) Antibody

20-abx132085
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  • 100 ug
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Tissue Factor Pathway Inhibitor (TFPI) Antibody

20-abx132086
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  • 100 ug
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Tissue Factor Pathway Inhibitor (TFPI) Antibody

20-abx174804
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  • 1 mg
  • 200 ug

Tissue Factor Pathway Inhibitor (TFPI) Antibody

abx238634-100ug 100 ug
EUR 661.2

Tissue Factor Pathway Inhibitor (TFPI) Antibody

20-abx104947
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  • 100 ug
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Tissue Factor Pathway Inhibitor (TFPI) Antibody

20-abx116110
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  • 150 ul
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Tissue Factor Pathway Inhibitor (TFPI) Antibody

20-abx103325
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  • 100 ug
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  • 1 mg
  • 200 ug
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Tissue Factor Pathway Inhibitor (TFPI) Antibody

20-abx103326
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  • 100 ug
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  • 1 mg
  • 200 ug
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Tissue Factor Pathway Inhibitor (TFPI) Antibody

20-abx103328
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  • 100 ug
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Tissue Factor Pathway Inhibitor (TFPI) Antibody

20-abx103329
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  • 100 ug
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Tissue Factor Pathway Inhibitor (TFPI) Antibody

20-abx001368
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  • 100 ul
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Tissue Factor Pathway Inhibitor (TFPI) Antibody

abx001368-100l 100 µl
EUR 400

Tissue Factor Pathway Inhibitor (TFPI) Antibody

abx001368-20l 20 µl
EUR 175

Tissue Factor Pathway Inhibitor (TFPI) Antibody

abx001368-50l 50 µl
EUR 275

Tissue Factor Pathway Inhibitor (TFPI) Antibody

abx116110-100g 100 µg Ask for price

Tissue Factor Pathway Inhibitor (TFPI) Antibody

abx116110-10g 10 µg
EUR 612.5

Tissue Factor Pathway Inhibitor (TFPI) Antibody

abx116110-200g 200 µg Ask for price

Tissue Factor Pathway Inhibitor (TFPI) Antibody

abx129517-100l 100 µl
EUR 262.5

Tissue Factor Pathway Inhibitor (TFPI) Antibody

abx129517-1ml 1 ml
EUR 687.5

Tissue Factor Pathway Inhibitor (TFPI) Antibody

abx129517-200l 200 µl
EUR 325

Tissue Factor Pathway Inhibitor (TFPI) Antibody

abx104947-100g 100 µg
EUR 475

Tissue Factor Pathway Inhibitor (TFPI) Antibody

abx104947-20g 20 µg
EUR 212.5

Tissue Factor Pathway Inhibitor (TFPI) Antibody

abx104947-50g 50 µg
EUR 250

Tissue Factor Pathway Inhibitor (TFPI) Antibody

abx132084-100tests 100 tests
EUR 237.5

Tissue Factor Pathway Inhibitor (TFPI) Antibody

abx132085-01mg 0.1 mg
EUR 275

Tissue Factor Pathway Inhibitor (TFPI) Antibody

abx132086-01mg 0.1 mg
EUR 237.5

Tissue Factor Pathway Inhibitor (TFPI) Antibody

abx103325-100g 100 µg
EUR 475

Tissue Factor Pathway Inhibitor (TFPI) Antibody

abx103325-20g 20 µg
EUR 212.5

Tissue Factor Pathway Inhibitor (TFPI) Antibody

abx103325-50g 50 µg
EUR 250

Tissue Factor Pathway Inhibitor (TFPI) Antibody

abx103326-100g 100 µg
EUR 475

Tissue Factor Pathway Inhibitor (TFPI) Antibody

abx103326-20g 20 µg
EUR 212.5

Tissue Factor Pathway Inhibitor (TFPI) Antibody

abx103326-50g 50 µg
EUR 250

Tissue Factor Pathway Inhibitor (TFPI) Antibody

abx103328-100g 100 µg
EUR 725

Tissue Factor Pathway Inhibitor (TFPI) Antibody

abx103328-20g 20 µg
EUR 262.5

Tissue Factor Pathway Inhibitor (TFPI) Antibody

abx103328-50g 50 µg
EUR 337.5

Tissue Factor Pathway Inhibitor (TFPI) Antibody

abx103329-100g 100 µg
EUR 725

Tissue Factor Pathway Inhibitor (TFPI) Antibody

abx103329-20g 20 µg
EUR 262.5

Tissue Factor Pathway Inhibitor (TFPI) Antibody

abx103329-50g 50 µg
EUR 337.5

Introduction of mutations of K9R and Y11A to the propeptide facilitated dissociation of the cleaved propeptide from the catalytic area of MTG and energetic MTG with none contamination of the propeptide was obtained. The precise exercise of the energetic MTG was 22.7±2.6 U/mg. The profitable expression and purification of energetic MTG by utilizing the chimera protein of TEV protease and MTG zymogen with mutations within the propeptide can advance using MTG and the researches utilizing MTG mediated cross-linking reactions.

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