High-Yield Production of Active Recombinant S. simulans Lysostaphin Expressed in E. coli in a Laboratory Bioreactor

Staphylococcus aureus (S. aureus), which has developed multidrug resistance, results in many healthcare-associated infections leading to vital medical and financial losses. Due to this fact, the event of latest environment friendly methods to cope with these micro organism has been gaining significance. Lysostaphin is a peptidoglycan hydrolase that has appreciable potential as a bacteriocin.

Nonetheless, there have been few reported optimization and scale-up research of the lysostaphin bioproduction course of. Our preliminary outcomes have revealed that the composition of auto-induction media at 30 °C will increase the produced lysostaphin round 10-fold in shake flasks. On this examine, reaching greater yields for recombinant lysostaphin in E. coli at a laboratory scale has been the goal, via using auto-induction media.

Optimized medium composition and fermentation parameters have been transferred to a laboratory-scale bioreactor. The examined circumstances improved protein yields as much as 184 mg/L in a Three L stirred bioreactor and the productiveness was improved 2-fold compared to beforehand printed stories. Moreover, this examine additionally confirmed that lysostaphin is an efficient bacteriocin on each commercially accessible and remoted S. aureus strains. These outcomes will contribute to future larger-scale manufacturing of lysostaphin through the proposed fermentation circumstances.

Combinatorial ethanol remedy will increase the general productiveness of recombinant hG-CSF in E. coli: a comparative examine

Human granulocyte colony-stimulating issue (hG-CSF) is a cytokine that regulates the proliferation, maturation, and differentiation of precursor cells to neutrophils. Within the current examine, we report the feasibility of inducing recombinant hG-CSF expression (rhG-CSF) in a pET vector system by combinatorial induction utilizing low-concentration ethanol, IPTG, and lactose and auto-induction media (AIM). The coding sequence of hG-CSF transcript variant 2 was expressed in pET14 vector, and the impact of combinatorial induction was analyzed on inclusion physique (IB) formation, biomass, protein purification, and bioactivity.

Outcomes confirmed that there was an inverse relationship between the temperature and soluble expression of rhG-CSF. Three-step washing with Triton-X, 2 M, and 5 M urea resulted within the most restoration of IBs. Combinatorial single-spike induction with IPTG, ethanol, and lactose in a batch tradition led to a 3-fold enhance within the expression of rhG-CSF. It was additionally noticed that low focus of ethanol (1-3% v/v) may very well be utilized in lieu of IPTG for inducing the rhG-CSF protein expression with out adversely affecting biomass manufacturing. A 2.4-fold enhance in productiveness was obtained in LB-AIM media with combinatorial ethanol induction, and the general yield of two.eight g/L rhG-CSF was discovered.

The purified rhG-CSF was bioactive and elevated the mobile proliferation of umbilical wire blood-derived mesenchymal stem cells (U-MSC) by 29%. In conclusion, our examine reveals that mixed ethanol induction can improve the expression of rhG-CSF with three-step washing for restoration of the proteins from IBs and a single-step purification of rhG-CSF by affinity chromatography. KEY POINTS: • Low focus of ethanol (1-3%) may very well be utilized in lieu of IPTG for inducing rhG-CSF expression. • Combinatorial single-spike induction with IPTG, ethanol, and lactose improved rhG-CSF expression. • Purified rhG-CSF was bioactive and elevated the proliferation of U-MSC.

Inhibition of E. coli host RNA Polymerase Permits Efficient Extracellular Recombinant Protein Manufacturing by Enhancing Outer Membrane Leakiness

Recombinant proteins in Escherichia coli are often expressed contained in the cell. With the rising curiosity in steady cultivation, secretion of product to the medium just isn’t solely a profit however a necessity in future bioprocessing. On this examine, we present that induced decoupling of development and heterologous gene expression within the E. coli X-press pressure, a pressure derived from BL21(DE3), facilitates extracellular recombinant protein manufacturing.

We investigated the impact of the method parameters temperature and particular glucose consumption price (q_S ) on development, productiveness, lysis and leakiness, to seek out the parameter area permitting extracellular protein manufacturing. Two mannequin proteins have been used, Protein A and a VHH single-domain antibody, and efficiency was in comparison with the economic commonplace pressure BL21(DE3).

We present that inducible development repression within the X-press pressure tremendously mitigates the impact of metabolic burden underneath completely different course of circumstances. Moreover, temperature and q_S have been used to regulate productiveness and leakiness. Within the X-press pressure, extracellular Protein A and VHH titer reached as much as 349 mg/g and 19.6 mg/g, respectively, comprising as much as 90% of the entire soluble product, whereas retaining cell lysis at a minimal.

Recombinant manufacturing of energetic microbial transglutaminase in E. coli by utilizing self-cleavable zymogen with mutated propeptide

Microbial transglutaminase from Streptomyces mobaraensis (MTG) has been broadly utilized in meals business and likewise in analysis and medical functions, since it could site-specifically modify proteins by the cross-linking response of glutamine residue and the first amino group. The recombinant expression system of MTG in E. coli offers higher accessibility for the researchers and thus can promote additional utilization of MTG.

Herein, we report manufacturing of energetic and soluble MTG in E. coli by utilizing a chimeric protein of tobacco etch virus (TEV) protease and MTG zymogen. A chimera of TEV protease and MTG zymogen with native propeptide resulted in energetic MTG contaminated with cleaved propeptide as a result of sturdy interplay between the propeptide and catalytic area of MTG.

Rat Tissue Factor Pathway Inhibitor (TFPI) ELISA Kit
DLR-TFPI-Ra-48T	DL Develop	48T	EUR 609.6
Description: A sandwich quantitative ELISA assay kit for detection of Rat Tissue Factor Pathway Inhibitor (TFPI) in samples from plasma, urine, tissue homogenates, cell culture supernates or other biological fluids.

Rat Tissue Factor Pathway Inhibitor (TFPI) ELISA Kit
DLR-TFPI-Ra-96T	DL Develop	96T	EUR 793.2
Description: A sandwich quantitative ELISA assay kit for detection of Rat Tissue Factor Pathway Inhibitor (TFPI) in samples from plasma, urine, tissue homogenates, cell culture supernates or other biological fluids.

Rabbit Tissue Factor Pathway Inhibitor (TFPI) ELISA Kit
DLR-TFPI-Rb-48T	DL Develop	48T	EUR 609.6
Description: A sandwich quantitative ELISA assay kit for detection of Rabbit Tissue Factor Pathway Inhibitor (TFPI) in samples from plasma.

Rabbit Tissue Factor Pathway Inhibitor (TFPI) ELISA Kit
DLR-TFPI-Rb-96T	DL Develop	96T	EUR 793.2
Description: A sandwich quantitative ELISA assay kit for detection of Rabbit Tissue Factor Pathway Inhibitor (TFPI) in samples from plasma.

Human Tissue Factor Pathway Inhibitor (TFPI) ELISA Kit
RD-TFPI-Hu-48Tests	Reddot Biotech	48 Tests	EUR 440.4

Human Tissue Factor Pathway Inhibitor (TFPI) ELISA Kit
RD-TFPI-Hu-96Tests	Reddot Biotech	96 Tests	EUR 602.4

Rat Tissue Factor Pathway Inhibitor (TFPI) ELISA Kit
RD-TFPI-Ra-48Tests	Reddot Biotech	48 Tests	EUR 613.2

Rat Tissue Factor Pathway Inhibitor (TFPI) ELISA Kit
RD-TFPI-Ra-96Tests	Reddot Biotech	96 Tests	EUR 850.8

Rabbit Tissue Factor Pathway Inhibitor (TFPI) ELISA Kit
RD-TFPI-Rb-48Tests	Reddot Biotech	48 Tests	EUR 613.2

Rabbit Tissue Factor Pathway Inhibitor (TFPI) ELISA Kit
RD-TFPI-Rb-96Tests	Reddot Biotech	96 Tests	EUR 850.8

Human Tissue Factor Pathway Inhibitor (TFPI) ELISA Kit
RDR-TFPI-Hu-48Tests	Reddot Biotech	48 Tests	EUR 459.6

Human Tissue Factor Pathway Inhibitor (TFPI) ELISA Kit
RDR-TFPI-Hu-96Tests	Reddot Biotech	96 Tests	EUR 630

Rat Tissue Factor Pathway Inhibitor (TFPI) ELISA Kit
RDR-TFPI-Ra-48Tests	Reddot Biotech	48 Tests	EUR 640.8

Rat Tissue Factor Pathway Inhibitor (TFPI) ELISA Kit
RDR-TFPI-Ra-96Tests	Reddot Biotech	96 Tests	EUR 890.4

Rabbit Tissue Factor Pathway Inhibitor (TFPI) ELISA Kit
RDR-TFPI-Rb-48Tests	Reddot Biotech	48 Tests	EUR 640.8

Rabbit Tissue Factor Pathway Inhibitor (TFPI) ELISA Kit
RDR-TFPI-Rb-96Tests	Reddot Biotech	96 Tests	EUR 890.4

CLIA kit for Mouse TFPI (Tissue Factor Pathway Inhibitor)
E-CL-M0650	Elabscience Biotech	1 plate of 96 wells	EUR 700.8
Description: A sandwich CLIA kit for quantitative measurement of Mouse TFPI (Tissue Factor Pathway Inhibitor) in samples from Serum, Plasma, Cell supernatant

ELISA kit for Mouse TFPI (Tissue Factor Pathway Inhibitor)
E-EL-M1162	Elabscience Biotech	1 plate of 96 wells	EUR 640.8
Description: A sandwich ELISA kit for quantitative measurement of Mouse TFPI (Tissue Factor Pathway Inhibitor) in samples from Serum, Plasma, Cell supernatant

Tissue Factor Pathway Inhibitor (TFPI) Antibody
20-abx132084	Abbexa	EUR 360.00 EUR 844.80 EUR 427.20 EUR 184.80 EUR 292.80	100 ug 1 mg 200 ug 20 ug 50 ug

Tissue Factor Pathway Inhibitor (TFPI) Antibody
20-abx132085	Abbexa	EUR 393.60 EUR 1011.60 EUR 526.80 EUR 184.80 EUR 309.60	100 ug 1 mg 200 ug 20 ug 50 ug

Tissue Factor Pathway Inhibitor (TFPI) Antibody
20-abx132086	Abbexa	EUR 360.00 EUR 844.80 EUR 427.20 EUR 184.80 EUR 292.80	100 ug 1 mg 200 ug 20 ug 50 ug

Tissue Factor Pathway Inhibitor (TFPI) Antibody
20-abx104947	Abbexa	EUR 376.80 EUR 159.60 EUR 994.80 EUR 526.80 EUR 326.40	100 ug 10 ug 1 mg 200 ug 50 ug

Tissue Factor Pathway Inhibitor (TFPI) Antibody
20-abx103325	Abbexa	EUR 376.80 EUR 159.60 EUR 994.80 EUR 526.80 EUR 326.40	100 ug 10 ug 1 mg 200 ug 50 ug

Tissue Factor Pathway Inhibitor (TFPI) Antibody
20-abx103326	Abbexa	EUR 376.80 EUR 159.60 EUR 994.80 EUR 526.80 EUR 326.40	100 ug 10 ug 1 mg 200 ug 50 ug

Tissue Factor Pathway Inhibitor (TFPI) Antibody
20-abx103328	Abbexa	EUR 510.00 EUR 159.60 EUR 1412.40 EUR 693.60 EUR 393.60	100 ug 10 ug 1 mg 200 ug 50 ug

Tissue Factor Pathway Inhibitor (TFPI) Antibody
20-abx103329	Abbexa	EUR 510.00 EUR 159.60 EUR 1412.40 EUR 693.60 EUR 393.60	100 ug 10 ug 1 mg 200 ug 50 ug

Tissue Factor Pathway Inhibitor (TFPI) Antibody
20-abx001368	Abbexa	EUR 493.20 EUR 710.40 EUR 218.40 EUR 376.80	100 ul 200 ul 20 ul 50 ul

Tissue Factor Pathway Inhibitor (TFPI) Antibody
20-abx116110	Abbexa	EUR 878.40 EUR 477.60	150 ul 50 ul

Tissue Factor Pathway Inhibitor (TFPI) Antibody
20-abx129517	Abbexa	EUR 493.20 EUR 159.60 EUR 1362.00 EUR 661.20 EUR 376.80	100 ug 10 ug 1 mg 200 ug 50 ug

Tissue Factor Pathway Inhibitor (TFPI) Protein
20-abx261170	Abbexa	EUR 4101.60 EUR 393.60 EUR 276.00	1 mg 20 ug 5 ug

Tissue Factor Pathway Inhibitor (TFPI) Antibody
20-abx323593	Abbexa	EUR 376.80 EUR 292.80	100 ug 50 ug

Tissue Factor Pathway Inhibitor (TFPI) Antibody
abx433382-200ul	Abbexa	200 ul	EUR 460.8

Tissue Factor Pathway Inhibitor (TFPI) Antibody
20-abx174804	Abbexa	EUR 1011.60 EUR 526.80	1 mg 200 ug

Tissue Factor Pathway Inhibitor (TFPI) Antibody
abx238634-100ug	Abbexa	100 ug	EUR 661.2

Rabbit Tissue factor pathway inhibitor (TFPI)
1-CSB-MP023437RB	Cusabio	EUR 466.80 EUR 1672.80 EUR 675.60 EUR 1230.00	100ug 1MG 200ug 500ug
Description: Recombinant Rabbit Tissue factor pathway inhibitor(TFPI) expressed in Mammalian cell

Rabbit Tissue factor pathway inhibitor (TFPI)
1-CSB-BP023437RB	Cusabio	EUR 1008.00 EUR 398.40 EUR 2604.00 EUR 1362.00 EUR 1894.80 EUR 564.00	100ug 10ug 1MG 200ug 500ug 50ug
Description: Recombinant Rabbit Tissue factor pathway inhibitor(TFPI) expressed in Baculovirus

Human Tissue factor pathway inhibitor (TFPI)
1-CSB-EP023437HU	Cusabio	EUR 456.00 EUR 256.80 EUR 1570.80 EUR 672.00 EUR 1047.60 EUR 314.40	100ug 10ug 1MG 200ug 500ug 50ug
Description: Recombinant Human Tissue factor pathway inhibitor(TFPI),partial expressed in E.coli

Rabbit Tissue factor pathway inhibitor (TFPI)
1-CSB-EP023437RB	Cusabio	EUR 733.20 EUR 370.80 EUR 2192.40 EUR 1126.80 EUR 1461.60 EUR 476.40	100ug 10ug 1MG 200ug 500ug 50ug
Description: Recombinant Rabbit Tissue factor pathway inhibitor(TFPI) expressed in E.coli

Rabbit Tissue factor pathway inhibitor (TFPI)
1-CSB-YP023437RB	Cusabio	EUR 814.80 EUR 402.00 EUR 2606.40 EUR 1261.20 EUR 1730.40 EUR 522.00	100ug 10ug 1MG 200ug 500ug 50ug
Description: Recombinant Rabbit Tissue factor pathway inhibitor(TFPI) expressed in Yeast

Recombinant Tissue Factor Pathway Inhibitor (TFPI)
4-RPA394Hu01	Cloud-Clone	EUR 571.58 EUR 276.00 EUR 1813.44 EUR 684.48 EUR 1248.96 EUR 458.40 EUR 4353.60	100 ug 10ug 1 mg 200 ug 500 ug 50ug 5 mg
Description: Recombinant Human Tissue Factor Pathway Inhibitor expressed in: E.coli

Recombinant Tissue Factor Pathway Inhibitor (TFPI)
4-RPA394Hu02	Cloud-Clone	EUR 571.58 EUR 276.00 EUR 1813.44 EUR 684.48 EUR 1248.96 EUR 458.40 EUR 4353.60	100 ug 10ug 1 mg 200 ug 500 ug 50ug 5 mg
Description: Recombinant Human Tissue Factor Pathway Inhibitor expressed in: E.coli

Recombinant Tissue Factor Pathway Inhibitor (TFPI)
4-RPA394Hu03	Cloud-Clone	EUR 571.58 EUR 276.00 EUR 1813.44 EUR 684.48 EUR 1248.96 EUR 458.40 EUR 4353.60	100 ug 10ug 1 mg 200 ug 500 ug 50ug 5 mg
Description: Recombinant Human Tissue Factor Pathway Inhibitor expressed in: E.coli

Recombinant Tissue Factor Pathway Inhibitor (TFPI)
4-RPA394Mu01	Cloud-Clone	EUR 496.32 EUR 256.80 EUR 1531.20 EUR 590.40 EUR 1060.80 EUR 408.00 EUR 3648.00	100 ug 10ug 1 mg 200 ug 500 ug 50ug 5 mg
Description: Recombinant Mouse Tissue Factor Pathway Inhibitor expressed in: E.coli

Recombinant Tissue Factor Pathway Inhibitor (TFPI)
4-RPA394Ra01	Cloud-Clone	EUR 421.06 EUR 236.40 EUR 1248.96 EUR 496.32 EUR 872.64 EUR 357.60 EUR 2942.40	100 ug 10ug 1 mg 200 ug 500 ug 50ug 5 mg
Description: Recombinant Rat Tissue Factor Pathway Inhibitor expressed in: E.coli

Recombinant Tissue Factor Pathway Inhibitor (TFPI)
4-RPA394Rb01	Cloud-Clone	EUR 614.59 EUR 288.00 EUR 1974.72 EUR 738.24 EUR 1356.48 EUR 487.20 EUR 4756.80	100 ug 10ug 1 mg 200 ug 500 ug 50ug 5 mg
Description: Recombinant Rabbit Tissue Factor Pathway Inhibitor expressed in: E.coli

Mouse Tissue Factor Pathway Inhibitor (TFPI) Protein
20-abx167190	Abbexa	EUR 693.60 EUR 309.60 EUR 2064.00 EUR 828.00 EUR 510.00	100 ug 10 ug 1 mg 200 ug 50 ug

CLIA kit for Human TFPI (Tissue Factor Pathway Inhibitor)
E-CL-H0152	Elabscience Biotech	1 plate of 96 wells	EUR 700.8
Description: A sandwich CLIA kit for quantitative measurement of Human TFPI (Tissue Factor Pathway Inhibitor) in samples from Serum, Plasma, Cell supernatant

CLIA kit for Rat TFPI (Tissue Factor Pathway Inhibitor)
E-CL-R0651	Elabscience Biotech	1 plate of 96 wells	EUR 700.8
Description: A sandwich CLIA kit for quantitative measurement of Rat TFPI (Tissue Factor Pathway Inhibitor) in samples from Serum, Plasma, Cell supernatant

ELISA kit for Human TFPI (Tissue Factor Pathway Inhibitor)
E-EL-H0165	Elabscience Biotech	1 plate of 96 wells	EUR 640.8
Description: A sandwich ELISA kit for quantitative measurement of Human TFPI (Tissue Factor Pathway Inhibitor) in samples from Serum, Plasma, Cell supernatant

ELISA kit for Rat TFPI (Tissue Factor Pathway Inhibitor)
E-EL-R0983	Elabscience Biotech	1 plate of 96 wells	EUR 640.8
Description: A sandwich ELISA kit for quantitative measurement of Rat TFPI (Tissue Factor Pathway Inhibitor) in samples from Serum, Plasma, Cell supernatant

ELISA kit for Monkey TFPI (Tissue Factor Pathway Inhibitor)
E-EL-MK0964	Elabscience Biotech	1 plate of 96 wells	EUR 700.8
Description: A sandwich ELISA kit for quantitative measurement of Monkey TFPI (Tissue Factor Pathway Inhibitor) in samples from Serum, Plasma, Cell supernatant

Mouse Tissue Factor Pathway Inhibitor (TFPI) ELISA Kit
20-abx154740	Abbexa	EUR 7970.40 EUR 4250.40 EUR 990.00	10 × 96 tests 5 × 96 tests 96 tests

Mouse Tissue Factor Pathway Inhibitor (TFPI) ELISA Kit
20-abx054160	Abbexa	EUR 7970.40 EUR 4250.40 EUR 990.00	10 × 96 tests 5 × 96 tests 96 tests

Mouse Tissue Factor Pathway Inhibitor (TFPI) CLIA Kit
abx195578-96tests	Abbexa	96 tests	EUR 990

Mouse Tissue Factor Pathway Inhibitor (TFPI) ELISA Kit
abx254457-96tests	Abbexa	96 tests	EUR 801.6

Mouse Tissue Factor Pathway Inhibitor (TFPI) ELISA Kit
abx576269-96tests	Abbexa	96 tests	EUR 801.6

Mouse Tissue Factor Pathway Inhibitor (TFPI) CLIA Kit
20-abx190450	Abbexa	EUR 9567.60 EUR 5095.20 EUR 1177.20	10 × 96 tests 5 × 96 tests 96 tests

Mouse Tissue factor pathway inhibitor, TFPI ELISA Kit
CSB-E08262m-24T	Cusabio	1 plate of 24 wells	EUR 198
Description: Quantitativesandwich ELISA kit for measuring Mouse Tissue factor pathway inhibitor, TFPI in samples from serum, plasma, cell culture supernates, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Mouse Tissue factor pathway inhibitor, TFPI ELISA Kit
1-CSB-E08262m	Cusabio	EUR 843.60 EUR 5811.60 EUR 3084.00	1 plate of 96 wells 10 plates of 96 wells each 5 plates of 96 wells each
Description: Quantitativesandwich ELISA kit for measuring Mouse Tissue factor pathway inhibitor, TFPI in samples from serum, plasma, cell culture supernates, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Mouse Tissue factor pathway inhibitor,TFPI ELISA Kit
CN-02305M1	ChemNorm	96T	EUR 558

Mouse Tissue factor pathway inhibitor,TFPI ELISA Kit
CN-02305M2	ChemNorm	48T	EUR 378

Mouse TFPI(Tissue Factor Pathway Inhibitor) ELISA Kit
EM1398	FN Test	96T	EUR 628.92
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Mus ;Sensitivity: 46.875pg/ml

Mouse Tissue factor pathway inhibitor(TFPI)ELISA Kit
GA-E0281MS-48T	GenAsia Biotech	48T	EUR 403.2

Mouse Tissue factor pathway inhibitor(TFPI)ELISA Kit
GA-E0281MS-96T	GenAsia Biotech	96T	EUR 640.8

Tissue Factor Pathway Inhibitor (TFPI) Polyclonal Antibody (Mouse)
4-PAA394Mu01	Cloud-Clone	EUR 283.20 EUR 2805.60 EUR 703.20 EUR 352.80 EUR 250.80	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Mouse Tissue Factor Pathway Inhibitor (TFPI)

Mouse Tissue Factor Pathway Inhibitor (TFPI) ELISA Kit
SEA394Mu-10x96wellstestplate	Cloud-Clone	10x96-wells test plate	EUR 5269.39
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Tissue Factor Pathway Inhibitor (TFPI) in Plasma, urine, cell culture supernates and other biological fluids.

Mouse Tissue Factor Pathway Inhibitor (TFPI) ELISA Kit
SEA394Mu-1x48wellstestplate	Cloud-Clone	1x48-wells test plate	EUR 539.12
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Tissue Factor Pathway Inhibitor (TFPI) in Plasma, urine, cell culture supernates and other biological fluids.

Mouse Tissue Factor Pathway Inhibitor (TFPI) ELISA Kit
SEA394Mu-1x96wellstestplate	Cloud-Clone	1x96-wells test plate	EUR 718.75
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Tissue Factor Pathway Inhibitor (TFPI) in Plasma, urine, cell culture supernates and other biological fluids.

Mouse Tissue Factor Pathway Inhibitor (TFPI) ELISA Kit
SEA394Mu-5x96wellstestplate	Cloud-Clone	5x96-wells test plate	EUR 2874.38
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Tissue Factor Pathway Inhibitor (TFPI) in Plasma, urine, cell culture supernates and other biological fluids.

Mouse Tissue Factor Pathway Inhibitor (TFPI) ELISA Kit
4-SEA394Mu	Cloud-Clone	EUR 5330.40 EUR 2815.20 EUR 718.80	10 plates of 96 wells 5 plates of 96 wells 1 plate of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Mouse Tissue Factor Pathway Inhibitor (TFPI) in samples from Plasma, urine, cell culture supernates and other biological fluids with no significant corss-reactivity with analogues from other species.

Mouse Tissue Factor Pathway Inhibitor (TFPI)CLIA Kit
SCA394Mu-10x96wellstestplate	Cloud-Clone	10x96-wells test plate	EUR 6965.86
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Mouse Tissue Factor Pathway Inhibitor (TFPI) in Plasma, urine, cell culture supernates and other biological fluids.

Mouse Tissue Factor Pathway Inhibitor (TFPI)CLIA Kit
SCA394Mu-1x48wellstestplate	Cloud-Clone	1x48-wells test plate	EUR 678.84
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Mouse Tissue Factor Pathway Inhibitor (TFPI) in Plasma, urine, cell culture supernates and other biological fluids.

Mouse Tissue Factor Pathway Inhibitor (TFPI)CLIA Kit
SCA394Mu-1x96wellstestplate	Cloud-Clone	1x96-wells test plate	EUR 918.34
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Mouse Tissue Factor Pathway Inhibitor (TFPI) in Plasma, urine, cell culture supernates and other biological fluids.

Mouse Tissue Factor Pathway Inhibitor (TFPI)CLIA Kit
SCA394Mu-5x96wellstestplate	Cloud-Clone	5x96-wells test plate	EUR 3772.51
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Mouse Tissue Factor Pathway Inhibitor (TFPI) in Plasma, urine, cell culture supernates and other biological fluids.

Mouse Tissue Factor Pathway Inhibitor (TFPI) CLIA Kit
4-SCA394Mu	Cloud-Clone	EUR 7026.00 EUR 3772.80 EUR 919.20	10 plates of 96 wells 5 plates of 96 wells 1 plate of 96 wells
Description: Double-antibody Sandwich chemiluminescent immunoassay for detection of Mouse Tissue Factor Pathway Inhibitor (TFPI)Plasma, urine, cell culture supernates and other biological fluids

Mouse Tissue factor pathway inhibitor(TFPI)ELISA Kit
QY-E21471	Qayee Biotechnology	96T	EUR 433.2

Mouse Tissue factor pathway inhibitor,TFPI ELISA Kit
YLA0745MO-48T	Shanghai YL Biotech	48T	EUR 465

Mouse Tissue factor pathway inhibitor,TFPI ELISA Kit
YLA0745MO-96T	Shanghai YL Biotech	96T	EUR 600

Human Tissue Factor Pathway Inhibitor (TFPI) Antibody
11621-05011	AssayPro	150 ug	EUR 260.4

Human Tissue Factor Pathway Inhibitor (TFPI) Protein
20-abx069318	Abbexa	EUR 794.40 EUR 326.40 EUR 2448.00 EUR 944.40 EUR 577.20	100 ug 10 ug 1 mg 200 ug 50 ug

Rat Tissue Factor Pathway Inhibitor (TFPI) Protein
20-abx069319	Abbexa	EUR 594.00 EUR 292.80 EUR 1696.80 EUR 693.60 EUR 427.20	100 ug 10 ug 1 mg 200 ug 50 ug

Rabbit Tissue Factor Pathway Inhibitor (TFPI) Protein
20-abx069320	Abbexa	EUR 861.60 EUR 343.20 EUR 2665.20 EUR 1028.40 EUR 610.80	100 ug 10 ug 1 mg 200 ug 50 ug

Human Tissue Factor Pathway Inhibitor (TFPI) Protein
20-abx069321	Abbexa	EUR 794.40 EUR 326.40 EUR 2448.00 EUR 944.40 EUR 577.20	100 ug 10 ug 1 mg 200 ug 50 ug

Human Tissue Factor Pathway Inhibitor (TFPI) Protein
20-abx069322	Abbexa	EUR 794.40 EUR 326.40 EUR 2448.00 EUR 944.40 EUR 577.20	100 ug 10 ug 1 mg 200 ug 50 ug

Tissue Factor Pathway Inhibitor (TFPI) Antibody Pair
20-abx370061	Abbexa	EUR 2047.20 EUR 1312.80	10 × 96 tests 5 × 96 tests

Tissue Factor Pathway Inhibitor (TFPI) Antibody Pair
20-abx370161	Abbexa	EUR 1946.40 EUR 1245.60	10 × 96 tests 5 × 96 tests

Tissue Factor Pathway Inhibitor (TFPI) Antibody Pair
20-abx370357	Abbexa	EUR 1412.40 EUR 927.60	10 × 96 tests 5 × 96 tests

Tissue Factor Pathway Inhibitor (TFPI) Antibody Pair
20-abx370374	Abbexa	EUR 2047.20 EUR 1312.80	10 × 96 tests 5 × 96 tests

Tissue Factor Pathway Inhibitor (TFPI) Antibody (FITC)
20-abx274012	Abbexa	EUR 543.60 EUR 292.80 EUR 1579.20 EUR 760.80 EUR 410.40	100 ug 10 ug 1 mg 200 ug 50 ug

Tissue Factor Pathway Inhibitor (TFPI) Antibody (FITC)
20-abx274146	Abbexa	EUR 560.40 EUR 292.80 EUR 1663.20 EUR 777.60 EUR 427.20	100 ug 10 ug 1 mg 200 ug 50 ug

Tissue Factor Pathway Inhibitor (TFPI) Antibody (Biotin)
20-abx274501	Abbexa	EUR 376.80 EUR 243.60 EUR 910.80 EUR 493.20 EUR 326.40	100 ug 10 ug 1 mg 200 ug 50 ug

Tissue Factor Pathway Inhibitor (TFPI) Antibody (Biotin)
20-abx272693	Abbexa	EUR 510.00 EUR 276.00 EUR 1462.80 EUR 710.40 EUR 393.60	100 ug 10 ug 1 mg 200 ug 50 ug

Tissue Factor Pathway Inhibitor (TFPI) Antibody (Biotin)
20-abx273169	Abbexa	EUR 543.60 EUR 292.80 EUR 1545.60 EUR 744.00 EUR 410.40	100 ug 10 ug 1 mg 200 ug 50 ug

Tissue Factor Pathway Inhibitor (TFPI) Antibody (Biotin)
20-abx271976	Abbexa	EUR 393.60 EUR 260.40 EUR 1078.80 EUR 543.60 EUR 343.20	100 ug 10 ug 1 mg 200 ug 50 ug

Mouse tissue factor pathway inhibitor (TFPI) Control/blocking peptide
AB-23005-P	Alpha Diagnostics	100ug	EUR 196.8

ELISA kit for Mouse TFPI (Tissue Factor Pathway Inhibitor)
ELK1474	ELK Biotech	1 plate of 96 wells	EUR 518.4
Description: A sandwich ELISA kit for detection of Tissue Factor Pathway Inhibitor from Mouse in samples from blood, serum, plasma, cell culture fluid and other biological fluids.

Tissue Factor Pathway Inhibitor (TFPI) Polyclonal Antibody (Mouse), APC
4-PAA394Mu01-APC	Cloud-Clone	EUR 394.80 EUR 3649.20 EUR 1024.80 EUR 499.20 EUR 254.40	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Mouse Tissue Factor Pathway Inhibitor (TFPI). This antibody is labeled with APC.

Tissue Factor Pathway Inhibitor (TFPI) Polyclonal Antibody (Mouse), Biotinylated
4-PAA394Mu01-Biotin	Cloud-Clone	EUR 358.80 EUR 2745.60 EUR 820.80 EUR 435.60 EUR 256.80	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Mouse Tissue Factor Pathway Inhibitor (TFPI). This antibody is labeled with Biotin.

Tissue Factor Pathway Inhibitor (TFPI) Polyclonal Antibody (Mouse), Cy3
4-PAA394Mu01-Cy3	Cloud-Clone	EUR 476.40 EUR 4815.60 EUR 1316.40 EUR 615.60 EUR 289.20	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Mouse Tissue Factor Pathway Inhibitor (TFPI). This antibody is labeled with Cy3.

Tissue Factor Pathway Inhibitor (TFPI) Polyclonal Antibody (Mouse), FITC
4-PAA394Mu01-FITC	Cloud-Clone	EUR 339.60 EUR 2942.40 EUR 843.60 EUR 423.60 EUR 226.80	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Mouse Tissue Factor Pathway Inhibitor (TFPI). This antibody is labeled with FITC.

Tissue Factor Pathway Inhibitor (TFPI) Polyclonal Antibody (Mouse), HRP
4-PAA394Mu01-HRP	Cloud-Clone	EUR 362.40 EUR 3182.40 EUR 907.20 EUR 452.40 EUR 240.00	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Mouse Tissue Factor Pathway Inhibitor (TFPI). This antibody is labeled with HRP.

Tissue Factor Pathway Inhibitor (TFPI) Polyclonal Antibody (Mouse), PE
4-PAA394Mu01-PE	Cloud-Clone	EUR 339.60 EUR 2942.40 EUR 843.60 EUR 423.60 EUR 226.80	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Mouse Tissue Factor Pathway Inhibitor (TFPI). This antibody is labeled with PE.

Rabbit Tissue Factor Pathway Inhibitor (TFPI) ELISA Kit
20-abx155111	Abbexa	EUR 8684.40 EUR 4626.00 EUR 1074.00	10 × 96 tests 5 × 96 tests 96 tests

Rat Tissue Factor Pathway Inhibitor (TFPI) ELISA Kit
20-abx156138	Abbexa	EUR 8684.40 EUR 4626.00 EUR 1074.00	10 × 96 tests 5 × 96 tests 96 tests

Human Tissue Factor Pathway Inhibitor (TFPI) ELISA Kit
20-abx153262	Abbexa	EUR 6373.20 EUR 3404.40 EUR 801.60	10 × 96 tests 5 × 96 tests 96 tests

Human Tissue Factor Pathway Inhibitor (TFPI) CLIA Kit
abx195577-96tests	Abbexa	96 tests	EUR 990

Rat Tissue Factor Pathway Inhibitor (TFPI) CLIA Kit
abx195579-96tests	Abbexa	96 tests	EUR 990

Chicken Tissue Factor Pathway Inhibitor (TFPI) ELISA Kit
abx356987-96tests	Abbexa	96 tests	EUR 990

Pig Tissue Factor Pathway Inhibitor (TFPI) ELISA Kit
abx360618-96tests	Abbexa	96 tests	EUR 990

Sheep Tissue Factor Pathway Inhibitor (TFPI) ELISA Kit
abx364322-96tests	Abbexa	96 tests	EUR 1111.2

Human Tissue Factor Pathway Inhibitor (TFPI) ELISA Kit
abx252213-96tests	Abbexa	96 tests	EUR 764.4

Introduction of mutations of K9R and Y11A to the propeptide facilitated dissociation of the cleaved propeptide from the catalytic area of MTG and energetic MTG with none contamination of the propeptide was obtained. The precise exercise of the energetic MTG was 22.7±2.6 U/mg. The profitable expression and purification of energetic MTG by utilizing the chimera protein of TEV protease and MTG zymogen with mutations within the propeptide can advance using MTG and the researches utilizing MTG mediated cross-linking reactions.