Generation of cell-permeant recombinant human transcription factor GATA4 from E. coli

Transcription issue GATA4 is expressed throughout early embryogenesis and is significant for correct improvement. As well as, it’s a essential reprogramming issue for deriving purposeful cardiomyocytes and was not too long ago recognized as a tumor suppressor protein in varied cancers. To generate a protected and efficient molecular instrument that may doubtlessly be utilized in a cell reprogramming course of and as an anti-cancer agent, now we have recognized optimum expression parameters to acquire soluble expression of human GATA4 in E. coli and purified the identical to homogeneity underneath native circumstances utilizing immobilized steel ion affinity chromatography.

The id of GATA4 protein was confirmed utilizing western blotting and mass spectrometry. Utilizing round dichroism spectroscopy, it was demonstrated that the purified recombinant protein has maintained its secondary construction, primarily comprising of random coils and α-helices. Subsequently, this purified recombinant protein was utilized to human cells and was discovered that it was non-toxic and capable of enter the cells in addition to translocate to the nucleus. Prospectively, this cell- and nuclear-permeant molecular instrument is appropriate for cell reprogramming experiments and generally is a protected and efficient therapeutic agent for most cancers remedy.

Immobilization of Recombinant Ecoli Cells in a Bacterial Cellulose-Silk Composite Matrix To Protect Organic Operate

Methods for the encapsulation of cells for the design of cell-based sensors require environment friendly immobilization procedures whereas preserving organic exercise of the reporter cells. Right here, we introduce an immobilization method that depends upon the symbiotic relationship between two bacterial strains: cellulose-producing Gluconacetobacter xylinus cells; and recombinant Escherichia coli cells harboring recombinase-based dual-color artificial riboswitch (RS), as a mannequin for cell-based sensor.

Following sequential coculturing of recombinant cells within the cellulose matrix, last immobilization of E. coli cells was accomplished after reconstituted silk fibroin (SF) protein was added to a “residing membrane” producing the composite bacterial cellulose-silk fibroin (BC-SF) scaffold. By controlling incubation parameters for each forms of cells, in addition to the conformations in SF secondary construction, a wide range of strong composite scaffolds have been ready starting from opaque to clear.

The properties of the scaffolds have been in contrast by way of porosity, water capability, distribution of recombinant cells inside the scaffolds matrix, onset of cells activation, and talent to guard recombinant operate of cells towards UV irradiation. The closer-fitted microstructure of clear BC-SF scaffolds resulted in leakage-free encapsulation of recombinant cells with preserved RS operate due to a mix of a number of parameters that intently matched properties of a biofilm surroundings.

Together with correct elasticity, positive porosity, capability to retain the water, and talent of SF to soak up UV gentle, the composite hydrogel materials supplied mandatory circumstances to type confined cell colonies that changed cell metabolism and enhanced cell resilience to the stresses induced by encapsulation.

Purification of recombinant human chemokine CCL2 in Ecoli and its operate in ovarian most cancers

Chemokine (CC-motif) ligand 2 (CCL2) is an inflammatory cytokine that regulates the infiltration and migration of monocytes. It’s extremely expressed by each tumor and stromal cells and has been related to tumorigenesis. Nonetheless, the impact of the exogenous administration of CCL2 on ovarian most cancers stays largely unknown. On this report, we tried to ascertain an expression system in Escherichia coli to provide recombinant hCCL2.

The recombinant plasmid containing the hCCL2 cDNA was ready utilizing the prokaryotic-expression plasmid pGEX-5X-Three and remodeled into E. coli BL21. GST-hCCL2 was efficiently induced by 0.1 mmol/L IPTG at 20 °C for six h, and the recombinant protein was purified utilizing affinity chromatography. The purified protein was recognized by SDS-PAGE and Western Blot.

Generation of cell-permeant recombinant human transcription factor GATA4 from E. coli

In vitro experiments revealed that rhCCL2 promoted the proliferation of ovarian most cancers cells and elevated the degrees of phosphorylation of MEK and ERK1/2, and the degrees of JUN, RELB and NF-κB2 mRNA. Moreover, inhibition of ERK signaling by remedy with PD98059 decreased ovarian most cancers cell proliferation and ranges of JUN, RELB, and NF-κB2 mRNA, indicating that exogenous rhCCL2 elevated the proliferation of ovarian most cancers cells, partially by activating the MAPK/ERK pathway, and by concentrating on JUN, RELB, and NF-κB2. Our research uncovered a selling function of exogenous CCL2 on ovarian most cancers cell proliferation by way of the MAPK/ERK signaling pathway, which can facilitate the invention of extra potential roles of CCL2 in ovarian most cancers.

Ethanol tolerance evaluation in recombinant Ecoli of ethanol responsive genes from Lactobacillus buchneri NRRL B-30929

We beforehand recognized particular proteins related to ethanol stress response in a Lactobacillus buchneri pressure able to rising in 10% ethanol. Within the present research, the distinctive roles of ethanol responsive genes are examined to find out if they’ll improve ethanol tolerance in E. coli host cells. The recombinant strains carrying ethanol responsive genes have been subjected to progress analyses in media with and with out 4% ethanol.

Among the many expression of those genes and progress analyses of the recombinant strains in ethanol, six genes Lbuc_0522 (NADPH-dependent methylglyoxal reductase), Lbuc_0354 (succinate semialdehyde dehydrogenase), Lbuc_1211(threonyl_tRNA synthetase), Lbuc_2051 (nitroreductase), Lbuc_0707 (branched chain amino acid aminotransferase) and Lbuc_1852 (proline-specific peptidase) conferred host cells tolerance to 4% ethanol.

Six genes Lbuc_1523 (phage main capsid protein, HK 97 household), Lbuc_1319 (phosphoglycerate kinase), Lbuc_0787 encoding fumarylacetoacetate hydrolase, Lbuc_1219 encoding UDP-N-acetylmuramate-L-alanine ligase, Lbuc_0466 encoding ornithine carbamoyltransferase and Lbuc_0858 encoding glycine hydroxymethyltransferase confirmed no affect on progress in media with 4% ethanol with IPTG induction when put next with E. coli carrying management pET28b plasmid.

Bovine Connective Tissue Growth Factor (CTGF) ELISA Kit

RDR-CTGF-b-48Tests 48 Tests
EUR 619.2

Bovine Connective Tissue Growth Factor (CTGF) ELISA Kit

RDR-CTGF-b-96T 96T
EUR 640.5
Description: serum, plasma, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids.

Bovine Connective Tissue Growth Factor (CTGF) ELISA Kit

RDR-CTGF-b-96Tests 96 Tests
EUR 859.2

Bovine Connective Tissue Growth Factor (CTGF) ELISA Kit

RD-CTGF-b-48T 48T
EUR 427
Description: serum, plasma, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids.

Bovine Connective Tissue Growth Factor (CTGF) ELISA Kit

RD-CTGF-b-48Tests 48 Tests
EUR 592.8

Bovine Connective Tissue Growth Factor (CTGF) ELISA Kit

RD-CTGF-b-96T 96T
EUR 610
Description: serum, plasma, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids.

Bovine Connective Tissue Growth Factor (CTGF) ELISA Kit

RD-CTGF-b-96Tests 96 Tests
EUR 820.8

Bovine Connective tissue growth factor (CTGF) ELISA Kit

RK06036 96T
EUR 280

Bovine Connective Tissue Growth Factor(CTGF) ELISA Kit

NSL0134Bo 96 T
EUR 528

Bovine CTGF/Connective tissue growth factor ELISA Kit

EK19167 96Т
EUR 650

Connective Tissue Growth Factor (CTGF) Polyclonal Antibody (Bovine)

4-PAA010Bo01
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Description: A Rabbit polyclonal antibody against Bovine Connective Tissue Growth Factor (CTGF)

Connective Tissue Growth Factor (CTGF) Polyclonal Antibody (Bovine), APC

4-PAA010Bo01-APC
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Description: A Rabbit polyclonal antibody against Bovine Connective Tissue Growth Factor (CTGF). This antibody is labeled with APC.

Connective Tissue Growth Factor (CTGF) Polyclonal Antibody (Bovine), Cy3

4-PAA010Bo01-Cy3
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Description: A Rabbit polyclonal antibody against Bovine Connective Tissue Growth Factor (CTGF). This antibody is labeled with Cy3.

Connective Tissue Growth Factor (CTGF) Polyclonal Antibody (Bovine), HRP

4-PAA010Bo01-HRP
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Description: A Rabbit polyclonal antibody against Bovine Connective Tissue Growth Factor (CTGF). This antibody is labeled with HRP.

Connective Tissue Growth Factor (CTGF) Polyclonal Antibody (Bovine), PE

4-PAA010Bo01-PE
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Description: A Rabbit polyclonal antibody against Bovine Connective Tissue Growth Factor (CTGF). This antibody is labeled with PE.

Connective Tissue Growth Factor (CTGF) Polyclonal Antibody (Bovine), FITC

4-PAA010Bo01-FITC
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Description: A Rabbit polyclonal antibody against Bovine Connective Tissue Growth Factor (CTGF). This antibody is labeled with FITC.

Connective Tissue Growth Factor (CTGF)

GWB-B2A544 0.02 mg Ask for price

Connective Tissue Growth Factor (CTGF) Polyclonal Antibody (Bovine), APC-Cy7

4-PAA010Bo01-APC-Cy7
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Description: A Rabbit polyclonal antibody against Bovine Connective Tissue Growth Factor (CTGF). This antibody is labeled with APC-Cy7.

Connective Tissue Growth Factor (CTGF) Polyclonal Antibody (Bovine), Biotinylated

4-PAA010Bo01-Biotin
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Description: A Rabbit polyclonal antibody against Bovine Connective Tissue Growth Factor (CTGF). This antibody is labeled with Biotin.

Human Connective tissue growth factor (CTGF)

1-CSB-EP006147HU
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Description: Recombinant Human Connective tissue growth factor(CTGF) expressed in E.coli

Human Connective tissue growth factor (CTGF)

1-CSB-EP006147HU1
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Description: Recombinant Human Connective tissue growth factor(CTGF),partial expressed in E.coli

Mouse Connective tissue growth factor (Ctgf)

1-CSB-EP006147MO
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Description: Recombinant Mouse Connective tissue growth factor (Ctgf),partial expressed in E.coli

Mouse Connective tissue growth factor (Ctgf)

1-CSB-EP006147MOe0
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Description: Recombinant Mouse Connective tissue growth factor(Ctgf),partial expressed in E.coli

Active Connective Tissue Growth Factor (CTGF)

APA010Ra01 10ug
EUR 182

Active Connective Tissue Growth Factor (CTGF)

4-APA010Ra01
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Description: Recombinant Rat Connective Tissue Growth Factor expressed in: Available from E.coli, Yeast, Baculovirus and Mammalian cells

Active Connective Tissue Growth Factor (CTGF)

RPU55435-100ug 100ug
EUR 562.1

Active Connective Tissue Growth Factor (CTGF)

RPU55435-1mg 1mg
EUR 2489.5

Active Connective Tissue Growth Factor (CTGF)

RPU55435-50ug 50ug
EUR 451

Connective Tissue Growth Factor (CTGF) Antibody

abx433502-200ul 200 ul
EUR 543.6

Connective Tissue Growth Factor (Ctgf) Antibody

20-abx319302
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  • 100 ug
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Connective Tissue Growth Factor (CTGF) Antibody

abx037800-100ug 100 ug
EUR 469.2

Connective Tissue Growth Factor (CTGF) Antibody

20-abx131768
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Connective Tissue Growth Factor (CTGF) Antibody

20-abx132193
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  • 100 ug
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Connective Tissue Growth Factor (CTGF) Antibody

20-abx132350
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  • 100 ug
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Connective Tissue Growth Factor (CTGF) Antibody

20-abx175995
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  • 1 mg
  • 200 ug

Connective Tissue Growth Factor (CTGF) Antibody

20-abx175996
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  • 1 mg
  • 200 ug

Connective Tissue Growth Factor (CTGF) Antibody

20-abx175997
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  • 1 mg
  • 200 ug

Connective Tissue Growth Factor (CTGF) Antibody

20-abx171909
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  • 1 mg
  • 200 ug

Connective Tissue Growth Factor (CTGF) Antibody

20-abx171910
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  • 1 mg
  • 200 ug

Connective tissue growth factor (CTGF) Antibody

20-abx214776
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  • 100 ul
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Connective Tissue Growth Factor (CTGF) Antibody

20-abx123700
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  • 100 ul
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Connective Tissue Growth Factor (CTGF) Antibody

20-abx125726
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Connective Tissue Growth Factor (CTGF) Antibody

20-abx125727
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Connective Tissue Growth Factor (CTGF) Antibody

20-abx125728
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Connective Tissue Growth Factor (CTGF) Antibody

abx232053-100ug 100 ug
EUR 610.8

Connective Tissue Growth Factor (CTGF) Antibody

abx232054-100ug 100 ug
EUR 610.8

Connective Tissue Growth Factor (CTGF) Antibody

20-abx225128
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Connective Tissue Growth Factor (CTGF) Antibody

20-abx109670
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  • 100 ug
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Connective Tissue Growth Factor (CTGF) Antibody

20-abx104836
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  • 100 ug
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Connective Tissue Growth Factor (CTGF) Antibody

20-abx103225
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  • 100 ug
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Connective Tissue Growth Factor (CTGF) Antibody

20-abx103226
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  • 100 ug
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Connective Tissue Growth Factor (CTGF) Antibody

20-abx103227
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  • 100 ug
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Connective Tissue Growth Factor (CTGF) Antibody

20-abx103228
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  • 100 ug
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Connective Tissue Growth Factor (CTGF) Antibody

20-abx103229
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  • 100 ug
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Connective Tissue Growth Factor (CTGF) Antibody

20-abx103230
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  • 100 ug
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Connective Tissue Growth Factor (CTGF) Antibody

20-abx103231
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Connective Tissue Growth Factor (CTGF) Antibody

20-abx132360
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Connective Tissue Growth Factor (CTGF) Antibody

abx027387-400ul 400 ul
EUR 627.6

Connective Tissue Growth Factor (CTGF) Antibody

abx027387-80l 80 µl
EUR 343.2

Connective Tissue Growth Factor (CTGF) Antibody

abx033675-400ul 400 ul
EUR 627.6

Connective Tissue Growth Factor (CTGF) Antibody

abx033675-80l 80 µl
EUR 343.2

Connective Tissue Growth Factor (CTGF) Antibody

abx033676-400ul 400 ul
EUR 627.6

Connective Tissue Growth Factor (CTGF) Antibody

abx033676-80l 80 µl
EUR 343.2

Connective Tissue Growth Factor (CTGF) Antibody

abx025473-100ul 100 ul
EUR 627.6

CTGF (untagged)-Human connective tissue growth factor (CTGF)

SC118973 10 µg Ask for price

Recombinant Connective Tissue Growth Factor (CTGF)

RPU40981-100ug 100ug
EUR 517

Recombinant Connective Tissue Growth Factor (CTGF)

RPU40981-1mg 1mg
EUR 2293.2

Recombinant Connective Tissue Growth Factor (CTGF)

RPU40981-50ug 50ug
EUR 415.8

Recombinant Connective Tissue Growth Factor (CTGF)

RPU40982-100ug 100ug
EUR 554.4

Recombinant Connective Tissue Growth Factor (CTGF)

RPU40982-1mg 1mg
EUR 2457

Recombinant Connective Tissue Growth Factor (CTGF)

RPU40982-50ug 50ug
EUR 445.5

Recombinant Connective Tissue Growth Factor (CTGF)

RPU40983-100ug 100ug
EUR 591.8

Recombinant Connective Tissue Growth Factor (CTGF)

RPU40983-1mg 1mg
EUR 2620.8

Recombinant Connective Tissue Growth Factor (CTGF)

RPU40983-50ug 50ug
EUR 475.2

Recombinant Connective Tissue Growth Factor (CTGF)

RPU40984-100ug 100ug
EUR 431.2

Recombinant Connective Tissue Growth Factor (CTGF)

RPU40984-1mg 1mg
EUR 1911

Recombinant Connective Tissue Growth Factor (CTGF)

RPU40984-50ug 50ug
EUR 346.5

Recombinant Connective Tissue Growth Factor (CTGF)

RPU40985-100ug 100ug
EUR 517

Recombinant Connective Tissue Growth Factor (CTGF)

RPU40985-1mg 1mg
EUR 2293.2

Recombinant Connective Tissue Growth Factor (CTGF)

RPU40985-50ug 50ug
EUR 415.8

Recombinant Connective Tissue Growth Factor (CTGF)

RPU40986-100ug 100ug
EUR 628.1

Recombinant Connective Tissue Growth Factor (CTGF)

RPU40986-1mg 1mg
EUR 2784.6

Recombinant Connective Tissue Growth Factor (CTGF)

RPU40986-50ug 50ug
EUR 504.9

Recombinant Connective Tissue Growth Factor (CTGF)

RPU54283-100ug 100ug
EUR 591.8

Recombinant Connective Tissue Growth Factor (CTGF)

RPU54283-1mg 1mg
EUR 2620.8

Recombinant Connective Tissue Growth Factor (CTGF)

RPU54283-50ug 50ug
EUR 475.2

Recombinant Connective Tissue Growth Factor (CTGF)

RPU59406-100ug 100ug
EUR 578

Recombinant Connective Tissue Growth Factor (CTGF)

RPU59406-1mg 1mg
EUR 2515.5

Recombinant Connective Tissue Growth Factor (CTGF)

RPU59406-50ug 50ug
EUR 412.8

Recombinant Connective Tissue Growth Factor (CTGF)

RPU57939-100ug 100ug
EUR 535.1

Recombinant Connective Tissue Growth Factor (CTGF)

RPU57939-1mg 1mg
EUR 2483.5

Recombinant Connective Tissue Growth Factor (CTGF)

RPU57939-50ug 50ug
EUR 415.9

Recombinant Connective Tissue Growth Factor (CTGF)

RPU58764-100ug 100ug
EUR 658.6

Recombinant Connective Tissue Growth Factor (CTGF)

RPU58764-1mg 1mg
EUR 3057.6

Recombinant Connective Tissue Growth Factor (CTGF)

RPU58764-50ug 50ug
EUR 485.1

Recombinant Connective Tissue Growth Factor (CTGF)

RPU58387-100ug 100ug
EUR 551.1

Recombinant Connective Tissue Growth Factor (CTGF)

RPU58387-1mg 1mg
EUR 2558.4

Recombinant Connective Tissue Growth Factor (CTGF)

RPU58387-50ug 50ug
EUR 428.5

Recombinant Connective Tissue Growth Factor (CTGF)

RPU56456-100ug 100ug
EUR 414.2

Recombinant Connective Tissue Growth Factor (CTGF)

RPU56456-1mg 1mg
EUR 1922

Recombinant Connective Tissue Growth Factor (CTGF)

RPU56456-50ug 50ug
EUR 295.7

Recombinant Connective Tissue Growth Factor (CTGF)

RPA010Bo01 10ug
EUR 192

Recombinant Connective Tissue Growth Factor (CTGF)

4-RPA010Bo01
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Description: Recombinant Bovine Connective Tissue Growth Factor expressed in: E.coli

Recombinant Connective Tissue Growth Factor (CTGF)

RPA010Cp01 10ug
EUR 159

Recombinant Connective Tissue Growth Factor (CTGF)

RPA010Ga01 10ug
EUR 164

Recombinant Connective Tissue Growth Factor (CTGF)

RPA010Hu01 10ug
EUR 168

Recombinant Connective Tissue Growth Factor (CTGF)

4-RPA010Hu01
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  • 100 ug
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  • 5 mg
Description: Recombinant Human Connective Tissue Growth Factor expressed in: E.coli

Recombinant Connective Tissue Growth Factor (CTGF)

RPA010Hu02 10ug
EUR 168

Recombinant Connective Tissue Growth Factor (CTGF)

4-RPA010Hu02
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  • 100 ug
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  • 5 mg
Description: Recombinant Human Connective Tissue Growth Factor expressed in: E.coli

Recombinant Connective Tissue Growth Factor (CTGF)

RPA010Hu03 10ug
EUR 172

Recombinant Connective Tissue Growth Factor (CTGF)

RPA010Hu04 10ug
EUR 170

Recombinant Connective Tissue Growth Factor (CTGF)

RPA010Mu01 10ug
EUR 180

Recombinant Connective Tissue Growth Factor (CTGF)

4-RPA010Mu01
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  • 100 ug
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  • 50ug
  • 5 mg
Description: Recombinant Mouse Connective Tissue Growth Factor expressed in: E.coli

Recombinant Connective Tissue Growth Factor (CTGF)

RPA010Ov01 10ug
EUR 204

Recombinant Connective Tissue Growth Factor (CTGF)

4-RPA010Ov01
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  • 100 ug
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  • 5 mg
Description: Recombinant Sheep Connective Tissue Growth Factor expressed in: E.coli

Recombinant Connective Tissue Growth Factor (CTGF)

RPA010Po01 10ug
EUR 192

The expression of two genes Lbuc_1557 (S-layer glycoprotein) and Lbuc_2157 (6-phosphogluconate dehydrogenase) resulted ethanol sensitivity phenotype.

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